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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names NOD/Lt-Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+ (Changed: 23-FEB-07 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse H2 Haplotype g7 Generation F1 (18-DEC-06) Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcDevelopment
This strain is an obligate double hemizygous F1 made by mating NOD/Lt-Tg(TCRaAI4)1Dvs (Stock #004334) and NOD/Lt-Tg(TCRbAI4)1Dvs (Stock #004335). This strain must be generated in this manner because mice carrying both the alpha and beta transgenes rapidly develop diabetes. (Graser et al., 2000.)
Related Strains
Strains carrying Tg(TcraAI4)1Dvs allele
006436 NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcraAI4)1Dvs/DvsJ 004259 NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+ 004347 NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/DvsJ 004334 NOD/ShiLt-Tg(TcraAI4)1Dvs View Strains carrying Tg(TcraAI4)1Dvs (4 strains)
006437 NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcrbAI4)1Dvs/DvsJ 004259 NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+ 004348 NOD.Cg-Rag1tm1Mom Tg(TcrbAI4)1Dvs/DvsJ 004335 NOD/ShiLt-Tg(TcrbAI4)1Dvs
Phenotype
Phenotype Information
assigned by genotype
Tg(TcraAI4)1Dvs/0 Tg(TcrbAI4)1Dvs/0
NOD-Tg(TcraAI4)1Dvs Tg(TcrbAI4)1Dvs
- immune system phenotype
- abnormal CD4-positive T cell morphology (MGI Ref ID J:93553)
- T cells expressing the transgenes comprise ~35% of the total peripheral blood lymphocyte population, a significant fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage
- abnormal CD8-positive T cell morphology (MGI Ref ID J:94192)
- only transgene expressing CD8+ T cells from primary splenocytes expand when cultured in presence of islets from NOD-scid donors
- abnormal cytotoxic T cell physiology (MGI Ref ID J:94192)
- transgenic CTLs generated in culture on islets exhibit cytotoxicity against a NOD-derived beta cell adenoma cell line and this is augmented when adenoma cells are pretreated with IFNG
- increased susceptibility to autoimmune diabetes (MGI Ref ID J:94192)
- female NOD mice carrying both transgenes develop IDDM at a greatly increased rate compared to nontransgenic controls with disease appearing as early as 3 weeks of age
- hematopoietic system phenotype
- abnormal CD4-positive T cell morphology (MGI Ref ID J:93553)
- T cells expressing the transgenes comprise ~35% of the total peripheral blood lymphocyte population, a significant fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage
- abnormal CD8-positive T cell morphology (MGI Ref ID J:94192)
- only transgene expressing CD8+ T cells from primary splenocytes expand when cultured in presence of islets from NOD-scid donors
This mouse can be used to support research in many areas including:
Tcra relatedResearch Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TcraAI4)1Dvs | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, David V Serreze | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | AI4aTg; | ||
| Mutation Made By | David Serreze, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| General Note |
In conjunction with Rag1tm1Mom
| ||
| Molecular Note | The rearranged Tcra transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgene contains a Tcra-V8/Tcra-J cDNA segment. [MGI Ref ID J:93553] | ||
| Allele Symbol | Tg(TcrbAI4)1Dvs | ||
| Allele Name | transgene insertion 1, David V Serreze | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | AI4bTg; | ||
| Mutation Made By | David Serreze, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| General Note |
In conjunction with Rag1tm1Mom
| ||
| Molecular Note | The rearranged Tcrb transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgenic construct contains a Tcrb-V2/Tcrb-D/Tcrb-J cDNA segment. [MGI Ref ID J:93553] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TcrbAI4)1Dvs, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754] [MGI Ref ID J:93553]
Tg(TcraAI4)1Dvs relatedTg(TcrbAI4)1Dvs relatedChen YG; Chen J; Osborne MA; Chapman HD; Besra GS; Porcelli SA; Leiter EH; Wilson SB; Serreze DV. 2006. CD38 is required for the peripheral survival of immunotolerogenic CD4+ invariant NK T cells in nonobese diabetic mice. J Immunol 177(5):2939-47. [PubMed: 16920929] [MGI Ref ID J:112615]
Chen YG; Silveira PA; Osborne MA; Chapman HD; Serreze DV. 2007. Cellular expression requirements for inhibition of type 1 diabetes by a dominantly protective major histocompatibility complex haplotype. Diabetes 56(2):424-30. [PubMed: 17259387] [MGI Ref ID J:121957]
DiLorenzo TP; Lieberman SM; Takaki T; Honda S; Chapman HD; Santamaria P; Serreze DV; Nathenson SG. 2002. During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities. Clin Immunol 105(3):332-41. [PubMed: 12498815] [MGI Ref ID J:94192]
Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754] [MGI Ref ID J:93553]
Leiter EH; Reifsnyder P; Driver J; Kamdar S; Choisy-Rossi C; Serreze DV; Hara M; Chervonsky A. 2007. Unexpected functional consequences of xenogeneic transgene expression in beta-cells of NOD mice. Diabetes Obes Metab 9 Suppl 2:14-22. [PubMed: 17919174] [MGI Ref ID J:127015]
Serreze DV; Choisy-Rossi CM; Grier AE; Holl TM; Chapman HD; Gahagan JR; Osborne MA; Zhang W; King BL; Brown A; Roopenian D; Marron MP. 2008. Through Regulation of TCR Expression Levels, an Idd7 Region Gene(s) Interactively Contributes to the Impaired Thymic Deletion of Autoreactive Diabetogenic CD8+ T Cells in Nonobese Diabetic Mice. J Immunol 180(5):3250-9. [PubMed: 18292549] [MGI Ref ID J:131557]
Tsui H; Chan Y; Tang L; Winer S; Cheung RK; Paltser G; Selvanantham T; Elford AR; Ellis JR; Becker DJ; Ohashi PS; Dosch HM. 2008. Targeting of pancreatic glia in type 1 diabetes. Diabetes 57(4):918-28. [PubMed: 18198358] [MGI Ref ID J:135214]
Chen YG; Chen J; Osborne MA; Chapman HD; Besra GS; Porcelli SA; Leiter EH; Wilson SB; Serreze DV. 2006. CD38 is required for the peripheral survival of immunotolerogenic CD4+ invariant NK T cells in nonobese diabetic mice. J Immunol 177(5):2939-47. [PubMed: 16920929] [MGI Ref ID J:112615]
DiLorenzo TP; Lieberman SM; Takaki T; Honda S; Chapman HD; Santamaria P; Serreze DV; Nathenson SG. 2002. During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities. Clin Immunol 105(3):332-41. [PubMed: 12498815] [MGI Ref ID J:94192]
Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754] [MGI Ref ID J:93553]
Leiter EH; Reifsnyder P; Driver J; Kamdar S; Choisy-Rossi C; Serreze DV; Hara M; Chervonsky A. 2007. Unexpected functional consequences of xenogeneic transgene expression in beta-cells of NOD mice. Diabetes Obes Metab 9 Suppl 2:14-22. [PubMed: 17919174] [MGI Ref ID J:127015]
Serreze DV; Choisy-Rossi CM; Grier AE; Holl TM; Chapman HD; Gahagan JR; Osborne MA; Zhang W; King BL; Brown A; Roopenian D; Marron MP. 2008. Through Regulation of TCR Expression Levels, an Idd7 Region Gene(s) Interactively Contributes to the Impaired Thymic Deletion of Autoreactive Diabetogenic CD8+ T Cells in Nonobese Diabetic Mice. J Immunol 180(5):3250-9. [PubMed: 18292549] [MGI Ref ID J:131557]
Tsui H; Chan Y; Tang L; Winer S; Cheung RK; Paltser G; Selvanantham T; Elford AR; Ellis JR; Becker DJ; Ohashi PS; Dosch HM. 2008. Targeting of pancreatic glia in type 1 diabetes. Diabetes 57(4):918-28. [PubMed: 18198358] [MGI Ref ID J:135214]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $.00 Female or Male Hemizygous for Tg(TcraAI4)1Dvs, Hemizygous for Tg(TcrbAI4)1Dvs
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $.00 Female or Male Hemizygous for Tg(TcraAI4)1Dvs, Hemizygous for Tg(TcrbAI4)1Dvs
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain. |
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General Terms and Conditions
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