Strain Name:

DBA/1-Abca1tm1Jdm/J

Stock Number:

003897

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;D1-Abca1tm1Jdm    (Changed: 15-DEC-04 )
Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator John D. McNeish,   Pfizer Central Research

Description
Mice that are homozygous for the Abca1-null allele are at greater risk of perinatal lethality. Autopsied pups exhibit perivisceral hemorrhaging. Pups that survive beyond birth have no detectable Abca1 gene transcript in the tissues of the liver. Homozygous females suffer from impaired placental development and are unable to produce litters. Both plasma lipids and lipoproteins are markedly reduced. Plasma cholesterol is decreased by approximately 70%. HDL-C and apoAI are decreased by greater than 99%. LDL-C and apoB are also reduced (70 % and 20%, respectively). Other characteristics observed are an increase in intestinal absorption of dietary cholesterol, an impaired ability for macrophages to engulf apoptotic cells and an accumulation of lipid rich macrophages and type II pneumocytes the lungs. These mice display pathophysiologic hallmarks similar to those associated with Tangier disease and provide a tool suitable for use in studies examining membrane lipid homeostasis.

Development
A targeting vector containing a neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 17 and 22 of the Abca1 gene. This portion of the gene encodes the the entire N-terminal ATP-binding cassette. The construct was electroporated into DBA/1LacJ-derived 252 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts to generate chimeric animals.

Control Information

  Control
   Wild-type from the colony
   001140 DBA/1LacJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Abca1
021067   B6.Cg-Abca1tm1Jp Abcg1tm1Tall/J
008596   B6.Cg-Tg(Prnp-Abca1)EHol/J
View Strains carrying other alleles of Abca1     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Tangier Disease; TGD
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
ATP-Binding Cassette, Subfamily A, Member 1; ABCA1   (ABCA1)
Hypercholesterolemia, Familial   (ABCA1)
Hypoalphalipoproteinemia, Primary   (ABCA1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Abca1tm1Jdm/Abca1+

        DBA/1LacJ-Abca1tm1Jdm
  • homeostasis/metabolism phenotype
  • abnormal lipid homeostasis
    • low apolipoprotein A-I level, reduced relative to wild-type on chow and Western-type diets   (MGI Ref ID J:61679)
    • low apolipoprotein B level, 20% reduction on chow diet   (MGI Ref ID J:61679)
    • abnormal circulating phospholipid level   (MGI Ref ID J:61679)
    • decreased circulating cholesterol level   (MGI Ref ID J:61679)
      • decreased circulating HDL cholesterol level
        • reduced relative to wild-type on chow and Western-type diets   (MGI Ref ID J:61679)
      • decreased circulating LDL cholesterol level
        • 20% reduction on chow diet   (MGI Ref ID J:61679)
  • respiratory system phenotype
  • abnormal lung morphology
    • microscopic, but not macroscopic, pale foci observed   (MGI Ref ID J:61679)
    • foci consisted of type II pneumocytes, lipid-laden macrophages, and cholesterol clefts   (MGI Ref ID J:61679)

Abca1tm1Jdm/Abca1tm1Jdm

        DBA/1LacJ-Abca1tm1Jdm
  • mortality/aging
  • partial perinatal lethality
    • incomplete penetrance   (MGI Ref ID J:61679)
    • at weaning, numbers of homozygotes recovered is reduced by about 50% from expected suggesting significant perinatal/postnatal lethality   (MGI Ref ID J:61679)
  • partial postnatal lethality
    • numbers of homozygous offspring recovered at weaning is significantly reduced from expected   (MGI Ref ID J:63265)
  • cardiovascular system phenotype
  • hemorrhage
    • perivisceral   (MGI Ref ID J:63265)
  • digestive/alimentary phenotype
  • increased intestinal cholesterol absorption
    • more cholesterol absorbed, relative to wild-type, on both chow and Western diets   (MGI Ref ID J:61679)
  • embryogenesis phenotype
  • abnormal placenta development   (MGI Ref ID J:63265)
  • endocrine/exocrine gland phenotype
  • enlarged adrenal glands   (MGI Ref ID J:60210)
  • growth/size/body phenotype
  • decreased body weight   (MGI Ref ID J:60210)
  • hematopoietic system phenotype
  • decreased platelet cell number   (MGI Ref ID J:60210)
  • enlarged spleen   (MGI Ref ID J:60210)
  • impaired macrophage phagocytosis
    • of apoptotic cells   (MGI Ref ID J:63265)
  • homeostasis/metabolism phenotype
  • abnormal fat-soluble vitamin level
    • decreased levels of fat-soluble vitamins in plasma   (MGI Ref ID J:60210)
  • abnormal lipid homeostasis
    • low apolipoprotein A-I level, 99.8% reduction relative to wild-type on chow diet and undetectable on Western-type diet   (MGI Ref ID J:61679)
    • low apolipoprotein B level, 70% reduction relative to wild-type on chow diet   (MGI Ref ID J:61679)
    • abnormal circulating phospholipid level   (MGI Ref ID J:61679)
    • decreased circulating cholesterol level   (MGI Ref ID J:60210)
      • ~70% reduction to wild-type on chow diet   (MGI Ref ID J:61679)
      • reduced relative to wild-type on Western-type diet   (MGI Ref ID J:61679)
      • decreased circulating HDL cholesterol level   (MGI Ref ID J:60210)
        • 99.5% reduction relative to wild-type on chow diet   (MGI Ref ID J:61679)
        • undetectable on Western-type diet   (MGI Ref ID J:61679)
      • decreased circulating LDL cholesterol level
        • 70% reduction relative to wild-type on chow diet   (MGI Ref ID J:61679)
    • increased intestinal cholesterol absorption
      • more cholesterol absorbed, relative to wild-type, on both chow and Western diets   (MGI Ref ID J:61679)
  • immune system phenotype
  • enlarged spleen   (MGI Ref ID J:60210)
  • impaired macrophage phagocytosis
    • of apoptotic cells   (MGI Ref ID J:63265)
  • reproductive system phenotype
  • female infertility
    • due to abnormal placental development   (MGI Ref ID J:63265)
  • respiratory system phenotype
  • abnormal lung morphology
    • macroscopic and microscopic pale foci in 5-10% of parenchyma in mice 7 months or older, increasingly prevalent with age   (MGI Ref ID J:61679)
    • foci consisted of type II pneumocytes, lipid-laden macrophages, and cholesterol clefts   (MGI Ref ID J:61679)
    • abnormal pulmonary alveolus morphology
      • abnormal architecture; alveolar septae are focally expanded by mild type II pneumocyte hypertrophy, macrophages, and aggregates of lymphocytes and plasma cells   (MGI Ref ID J:61679)
      • lipid accumulation within alveolar cells   (MGI Ref ID J:61679)

Abca1tm1Jdm/Abca1tm1Jdm

        DBA/1-Abca1tm1Jdm/J
  • cellular phenotype
  • increased cholesterol efflux   (MGI Ref ID J:130777)
  • homeostasis/metabolism phenotype
  • increased cholesterol efflux   (MGI Ref ID J:130777)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Abca1tm1Jdm/Abca1tm1Jdm

        involves: DBA/1LacJ
  • homeostasis/metabolism phenotype
  • abnormal circulating plant sterol level
    • strong reduction of plasma beta-sitosterol and campesterol levels   (MGI Ref ID J:87209)

Abca1tm1Jdm/Abca1tm1Jdm

        involves: C57BL/6 * DBA/1LacJ
  • mortality/aging
  • partial perinatal lethality
    • numbers of homozygous offspring recovered at weaning is significantly reduced from expected   (MGI Ref ID J:132254)
  • hematopoietic system phenotype
  • abnormal thymus morphology
    • massive thymic involution induced by dexamethasone treatment results in significant decrease in thymic weight and cellularity compared to wild-type, and similar to single null mice   (MGI Ref ID J:132254)
  • impaired macrophage phagocytosis
    • isolated peritoneal macrophages exposed to apoptotic thymocytes internalize similar numbers of cells (1-2 cells) as wild-type after 15-30 minutes, but this number does not increase with time as with wild-type which internalize 4-5 cells at 60-120 minutes   (MGI Ref ID J:132254)
    • after massive thymic involution induced by dexamethasone treatment, macrophages display reduced phagocytosis ability compared to wild-type, and similar to single-null mice   (MGI Ref ID J:132254)
  • immune system phenotype
  • abnormal thymus morphology
    • massive thymic involution induced by dexamethasone treatment results in significant decrease in thymic weight and cellularity compared to wild-type, and similar to single null mice   (MGI Ref ID J:132254)
  • impaired macrophage phagocytosis
    • isolated peritoneal macrophages exposed to apoptotic thymocytes internalize similar numbers of cells (1-2 cells) as wild-type after 15-30 minutes, but this number does not increase with time as with wild-type which internalize 4-5 cells at 60-120 minutes   (MGI Ref ID J:132254)
    • after massive thymic involution induced by dexamethasone treatment, macrophages display reduced phagocytosis ability compared to wild-type, and similar to single-null mice   (MGI Ref ID J:132254)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • circulating triglyceride levels are normal in fasting mice   (MGI Ref ID J:132254)
    • decreased circulating cholesterol level
      • levels are reduced compared to adult wild-type or Tgm2-null mice   (MGI Ref ID J:132254)
      • decreased circulating HDL cholesterol level
        • adult mice show nearly complete loss of HDL cholesterol in fasting mice   (MGI Ref ID J:132254)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Abca1tm1Jdm related

Cardiovascular Research
Hypocholesterolemia

Metabolism Research
Lipid Metabolism

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Abca1tm1Jdm
Allele Name targeted mutation 1, John D McNeish
Allele Type Targeted (knock-out)
Common Name(s) Abc1-; Abca1-; Abca1-;
Mutation Made By John McNeish,   Pfizer Central Research
Strain of OriginDBA/1LacJ
Gene Symbol and Name Abca1, ATP-binding cassette, sub-family A (ABC1), member 1
Chromosome 4
Gene Common Name(s) ABC-1; ABC1; ATP-binding cassette 1; Abc1; CERP; HDLDT1; TGD;
General Note When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777)
Molecular Note A 5.7 kb genomic fragment containing exons 17-22 was deleted and replaced with a neomycin selection cassette. These sequences correspond to amino acids 788 to 1093 of the encoded protein. RT-PCR analysis on RNA derived from liver demonstrated that no detectable transcript was produced from this allele in homozygous mice. [MGI Ref ID J:61679]

Genotyping

Genotyping Information

Genotyping Protocols

Abca1tm1Jdm, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hamon Y; Broccardo C; Chambenoit O; Luciani MF; Toti F; Chaslin S; Freyssinet JM; Devaux PF; McNeish J; Marguet D; Chimini G. 2000. ABC1 promotes engulfment of apoptotic cells and transbilayer redistribution of phosphatidylserine. Nat Cell Biol 2(7):399-406. [PubMed: 10878804]  [MGI Ref ID J:63265]

Additional References

Wahrle SE; Jiang H; Parsadanian M; Legleiter J; Han X; Fryer JD; Kowalewski T; Holtzman DM. 2004. ABCA1 is required for normal central nervous system ApoE levels and for lipidation of astrocyte-secreted apoE. J Biol Chem 279(39):40987-93. [PubMed: 15269217]  [MGI Ref ID J:93336]

Abca1tm1Jdm related

Adorni MP; Zimetti F; Billheimer JT; Wang N; Rader DJ; Phillips MC; Rothblat GH. 2007. The roles of different pathways in the release of cholesterol from macrophages. J Lipid Res 48(11):2453-62. [PubMed: 17761631]  [MGI Ref ID J:127358]

Bates SR; Tao JQ; Collins HL; Francone OL; Rothblat GH. 2005. Pulmonary abnormalities due to ABCA1 deficiency in mice. Am J Physiol Lung Cell Mol Physiol 289(6):L980-9. [PubMed: 16055479]  [MGI Ref ID J:105011]

Bravo I; Amigo L; Cohen DE; Nervi F; Rigotti A; Francone O; Zanlungo S. 2007. Role of plasma and liver cholesterol- and lipoprotein-metabolism determinants in LpX formation in the mouse. Biochim Biophys Acta 1770(6):979-88. [PubMed: 17399905]  [MGI Ref ID J:121757]

Brunham LR; Singaraja RR; Duong M; Timmins JM; Fievet C; Bissada N; Kang MH; Samra A; Fruchart JC; McManus B; Staels B; Parks JS; Hayden MR. 2009. Tissue-specific roles of ABCA1 influence susceptibility to atherosclerosis. Arterioscler Thromb Vasc Biol 29(4):548-54. [PubMed: 19201688]  [MGI Ref ID J:159775]

Burns MP; Vardanian L; Pajoohesh-Ganji A; Wang L; Cooper M; Harris DC; Duff K; Rebeck GW. 2006. The effects of ABCA1 on cholesterol efflux and Abeta levels in vitro and in vivo. J Neurochem 98(3):792-800. [PubMed: 16771834]  [MGI Ref ID J:119294]

Calpe-Berdiel L; Rotllan N; Palomer X; Ribas V; Blanco-Vaca F; Escola-Gil JC. 2005. Direct evidence in vivo of impaired macrophage-specific reverse cholesterol transport in ATP-binding cassette transporter A1-deficient mice. Biochim Biophys Acta 1738(1-3):6-9. [PubMed: 16413225]  [MGI Ref ID J:107452]

Combes V; Coltel N; Alibert M; van Eck M; Raymond C; Juhan-Vague I; Grau GE; Chimini G. 2005. ABCA1 gene deletion protects against cerebral malaria: potential pathogenic role of microparticles in neuropathology. Am J Pathol 166(1):295-302. [PubMed: 15632021]  [MGI Ref ID J:95235]

Coutinho JM; Singaraja RR; Kang M; Arenillas DJ; Bertram LN; Bissada N; Staels B; Fruchart JC; Fievet C; Joseph-George AM; Wasserman WW; Hayden MR. 2005. Complete functional rescue of the ABCA1-/- mouse by human BAC transgenesis. J Lipid Res 46(6):1113-23. [PubMed: 15772424]  [MGI Ref ID J:99808]

Cuchel M; Lund-Katz S; de la Llera-Moya M; Millar JS; Chang D; Fuki I; Rothblat GH; Phillips MC; Rader DJ. 2010. Pathways by which reconstituted high-density lipoprotein mobilizes free cholesterol from whole body and from macrophages. Arterioscler Thromb Vasc Biol 30(3):526-32. [PubMed: 20018934]  [MGI Ref ID J:172105]

Duka A; Fotakis P; Georgiadou D; Kateifides A; Tzavlaki K; von Eckardstein L; Stratikos E; Kardassis D; Zannis VI. 2013. ApoA-IV promotes the biogenesis of apoA-IV-containing HDL particles with the participation of ABCA1 and LCAT. J Lipid Res 54(1):107-15. [PubMed: 23132909]  [MGI Ref ID J:192256]

Fan J; Stukas S; Wong C; Chan J; May S; Devalle N; Hirsch-Reinshagen V; Wilkinson A; Oda MN; Wellington CL. 2011. An ABCA1-independent pathway for recycling a poorly lipidated 8.1 nm apolipoprotein E particle from glia. J Lipid Res 52(9):1605-16. [PubMed: 21705806]  [MGI Ref ID J:175557]

Fitz NF; Cronican AA; Saleem M; Fauq AH; Chapman R; Lefterov I; Koldamova R. 2012. Abca1 Deficiency Affects Alzheimer's Disease-Like Phenotype in Human ApoE4 But Not in ApoE3-Targeted Replacement Mice. J Neurosci 32(38):13125-36. [PubMed: 22993429]  [MGI Ref ID J:188282]

Francone OL; Royer L; Boucher G; Haghpassand M; Freeman A; Brees D; Aiello RJ. 2005. Increased cholesterol deposition, expression of scavenger receptors, and response to chemotactic factors in Abca1-deficient macrophages. Arterioscler Thromb Vasc Biol 25(6):1198-205. [PubMed: 15831807]  [MGI Ref ID J:114227]

Francone OL; Subbaiah PV; van Tol A; Royer L; Haghpassand M. 2003. Abnormal phospholipid composition impairs HDL biogenesis and maturation in mice lacking Abca1. Biochemistry 42(28):8569-78. [PubMed: 12859204]  [MGI Ref ID J:84572]

Gautier EL; Westerterp M; Bhagwat N; Cremers S; Shih A; Abdel-Wahab O; Lutjohann D; Randolph GJ; Levine RL; Tall AR; Yvan-Charvet L. 2013. HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders. J Exp Med 210(2):339-53. [PubMed: 23319699]  [MGI Ref ID J:195890]

Groen AK; Bloks VW; Bandsma RH; Ottenhoff R; Chimini G; Kuipers F. 2001. Hepatobiliary cholesterol transport is not impaired in Abca1-null mice lacking HDL. J Clin Invest 108(6):843-50. [PubMed: 11560953]  [MGI Ref ID J:71689]

Hirsch-Reinshagen V; Maia LF; Burgess BL; Blain JF; Naus KE; McIsaac SA; Parkinson PF; Chan JY; Tansley GH; Hayden MR; Poirier J; Van Nostrand W; Wellington CL. 2005. The absence of ABCA1 decreases soluble ApoE levels but does not diminish amyloid deposition in two murine models of Alzheimer disease. J Biol Chem 280(52):43243-56. [PubMed: 16207707]  [MGI Ref ID J:105900]

Hirsch-Reinshagen V; Zhou S; Burgess BL; Bernier L; McIsaac SA; Chan JY; Tansley GH; Cohn JS; Hayden MR; Wellington CL. 2004. Deficiency of ABCA1 impairs apolipoprotein E metabolism in brain. J Biol Chem 279(39):41197-207. [PubMed: 15269218]  [MGI Ref ID J:93331]

Hossain MA; Tsujita M; Akita N; Kobayashi F; Yokoyama S. 2009. Cholesterol homeostasis in ABCA1/LCAT double-deficient mouse. Biochim Biophys Acta 1791(12):1197-205. [PubMed: 19729076]  [MGI Ref ID J:164846]

Hu W; Abe-Dohmae S; Tsujita M; Iwamoto N; Ogikubo O; Otsuka T; Kumon Y; Yokoyama S. 2008. Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J Lipid Res 49(2):386-93. [PubMed: 18033752]  [MGI Ref ID J:131875]

Huang ZH; Fitzgerald ML; Mazzone T. 2006. Distinct cellular loci for the ABCA1-dependent and ABCA1-independent lipid efflux mediated by endogenous apolipoprotein E expression. Arterioscler Thromb Vasc Biol 26(1):157-62. [PubMed: 16254198]  [MGI Ref ID J:127981]

Iadevaia V; Rinaldi A; Falasca L; Pucillo LP; Alonzi T; Chimini G; Piacentini M. 2006. ATP-binding cassette transporter 1 and transglutaminase 2 act on the same genetic pathway in the apoptotic cell clearance. Cell Death Differ 13(11):1998-2001. [PubMed: 16691213]  [MGI Ref ID J:132254]

Iwamoto N; Abe-Dohmae S; Sato R; Yokoyama S. 2006. ABCA7 expression is regulated by cellular cholesterol through the SREBP2 pathway and associated with phagocytosis. J Lipid Res 47(9):1915-27. [PubMed: 16788211]  [MGI Ref ID J:114490]

Koldamova R; Staufenbiel M; Lefterov I. 2005. Lack of ABCA1 considerably decreases brain ApoE level and increases amyloid deposition in APP23 mice. J Biol Chem 280(52):43224-35. [PubMed: 16207713]  [MGI Ref ID J:105902]

Koseki M; Hirano K; Masuda D; Ikegami C; Tanaka M; Ota A; Sandoval JC; Nakagawa-Toyama Y; Sato SB; Kobayashi T; Shimada Y; Ohno-Iwashita Y; Matsuura F; Shimomura I; Yamashita S. 2007. Increased lipid rafts and accelerated lipopolysaccharide-induced tumor necrosis factor-alpha secretion in Abca1-deficient macrophages. J Lipid Res 48(2):299-306. [PubMed: 17079792]  [MGI Ref ID J:118227]

Kypreos KE. 2008. ABCA1 promotes the de novo biogenesis of apolipoprotein CIII-containing HDL particles in vivo and modulates the severity of apolipoprotein CIII-induced hypertriglyceridemia. Biochemistry 47(39):10491-502. [PubMed: 18767813]  [MGI Ref ID J:141173]

Kypreos KE; Zannis VI. 2007. Pathway of biogenesis of apolipoprotein E-containing HDL in vivo with the participation of ABCA1 and LCAT. Biochem J 403(2):359-67. [PubMed: 17206937]  [MGI Ref ID J:121679]

Lammers B; Zhao Y; Foks AC; Hildebrand RB; Kuiper J; Van Berkel TJ; Van Eck M. 2012. Leukocyte ABCA1 remains atheroprotective in splenectomized LDL receptor knockout mice. PLoS One 7(10):e48080. [PubMed: 23133551]  [MGI Ref ID J:192253]

Lammers B; Zhao Y; Hoekstra M; Hildebrand RB; Ye D; Meurs I; Van Berkel TJ; Van Eck M. 2011. Augmented atherogenesis in LDL receptor deficient mice lacking both macrophage ABCA1 and ApoE. PLoS One 6(10):e26095. [PubMed: 22022523]  [MGI Ref ID J:178098]

Lefterov I; Fitz NF; Cronican AA; Fogg A; Lefterov P; Kodali R; Wetzel R; Koldamova R. 2010. Apolipoprotein A-I deficiency increases cerebral amyloid angiopathy and cognitive deficits in APP/PS1DeltaE9 mice. J Biol Chem 285(47):36945-57. [PubMed: 20739292]  [MGI Ref ID J:166877]

Lindegaard ML; Wassif CA; Vaisman B; Amar M; Wasmuth EV; Shamburek R; Nielsen LB; Remaley AT; Porter FD. 2008. Characterization of placental cholesterol transport: ABCA1 is a potential target for in utero therapy of Smith-Lemli-Opitz syndrome. Hum Mol Genet 17(23):3806-13. [PubMed: 18775956]  [MGI Ref ID J:141147]

McNeish J; Aiello RJ; Guyot D; Turi T; Gabel C; Aldinger C; Hoppe KL; Roach ML; Royer LJ; de Wet J; Broccardo C; Chimini G; Francone OL. 2000. High density lipoprotein deficiency and foam cell accumulation in mice with targeted disruption of ATP-binding cassette transporter-1. Proc Natl Acad Sci U S A 97(8):4245-50. [PubMed: 10760292]  [MGI Ref ID J:61679]

Murphy AJ; Akhtari M; Tolani S; Pagler T; Bijl N; Kuo CL; Wang M; Sanson M; Abramowicz S; Welch C; Bochem AE; Kuivenhoven JA; Yvan-Charvet L; Tall AR. 2011. ApoE regulates hematopoietic stem cell proliferation, monocytosis, and monocyte accumulation in atherosclerotic lesions in mice. J Clin Invest 121(10):4138-49. [PubMed: 21968112]  [MGI Ref ID J:178453]

Murphy AJ; Bijl N; Yvan-Charvet L; Welch CB; Bhagwat N; Reheman A; Wang Y; Shaw JA; Levine RL; Ni H; Tall AR; Wang N. 2013. Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis. Nat Med 19(5):586-94. [PubMed: 23584088]  [MGI Ref ID J:198517]

Orso E; Broccardo C; Kaminski WE; Bottcher A; Liebisch G; Drobnik W; Gotz A; Chambenoit O; Diederich W; Langmann T; Spruss T; Luciani MF; Rothe G; Lackner KJ; Chimini G; Schmitz G. 2000. Transport of lipids from golgi to plasma membrane is defective in tangier disease patients and Abc1-deficient mice. Nat Genet 24(2):192-6. [PubMed: 10655069]  [MGI Ref ID J:60210]

Out R; Jessup W; Le Goff W; Hoekstra M; Gelissen IC; Zhao Y; Kritharides L; Chimini G; Kuiper J; Chapman MJ; Huby T; Van Berkel TJ; Van Eck M. 2008. Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporters A1 and G1. Circ Res 102(1):113-20. [PubMed: 17967783]  [MGI Ref ID J:141462]

Pagler TA; Wang M; Mondal M; Murphy AJ; Westerterp M; Moore KJ; Maxfield FR; Tall AR. 2011. Deletion of ABCA1 and ABCG1 impairs macrophage migration because of increased Rac1 signaling. Circ Res 108(2):194-200. [PubMed: 21148432]  [MGI Ref ID J:183506]

Pascual-Garcia M; Carbo JM; Leon T; Matalonga J; Out R; Van Berkel T; Sarrias MR; Lozano F; Celada A; Valledor AF. 2011. Liver X receptors inhibit macrophage proliferation through downregulation of cyclins D1 and B1 and cyclin-dependent kinases 2 and 4. J Immunol 186(8):4656-67. [PubMed: 21398609]  [MGI Ref ID J:172455]

Plosch T; Bloks VW; Terasawa Y; Berdy S; Siegler K; Van Der Sluijs F; Kema IP; Groen AK; Shan B; Kuipers F; Schwartz M. 2004. Sitosterolemia in ABC-Transporter G5-deficient mice is aggravated on activation of the liver-X receptor. Gastroenterology 126(1):290-300. [PubMed: 14699507]  [MGI Ref ID J:87209]

Plosch T; Kok T; Bloks VW; Smit MJ; Havinga R; Chimini G; Groen AK; Kuipers F. 2002. Increased hepatobiliary and fecal cholesterol excretion upon activation of the liver X receptor is independent of ABCA1. J Biol Chem 277(37):33870-7. [PubMed: 12105210]  [MGI Ref ID J:78949]

Pradel LC; Mitchell AJ; Zarubica A; Dufort L; Chasson L; Naquet P; Broccardo C; Chimini G. 2009. ATP-binding cassette transporter hallmarks tissue macrophages and modulates cytokine-triggered polarization programs. Eur J Immunol 39(8):2270-80. [PubMed: 19609977]  [MGI Ref ID J:151985]

Reboul E; Trompier D; Moussa M; Klein A; Landrier JF; Chimini G; Borel P. 2009. ATP-binding cassette transporter A1 is significantly involved in the intestinal absorption of alpha- and gamma-tocopherol but not in that of retinyl palmitate in mice. Am J Clin Nutr 89(1):177-84. [PubMed: 19056557]  [MGI Ref ID J:163801]

Samyn H; Moerland M; van Gent T; van Haperen R; van Tol A; de Crom R. 2009. Reduction of HDL levels lowers plasma PLTP and affects its distribution among lipoproteins in mice. Biochim Biophys Acta 1791(8):790-6. [PubMed: 19422933]  [MGI Ref ID J:153479]

Selva DM; Hirsch-Reinshagen V; Burgess B; Zhou S; Chan J; McIsaac S; Hayden MR; Hammond GL; Vogl AW; Wellington CL. 2004. The ATP-binding cassette transporter 1 mediates lipid efflux from Sertoli cells and influences male fertility. J Lipid Res 45(6):1040-50. [PubMed: 15026428]  [MGI Ref ID J:89906]

Singaraja RR; Stahmer B; Brundert M; Merkel M; Heeren J; Bissada N; Kang M; Timmins JM; Ramakrishnan R; Parks JS; Hayden MR; Rinninger F. 2006. Hepatic ATP-binding cassette transporter A1 is a key molecule in high-density lipoprotein cholesteryl ester metabolism in mice. Arterioscler Thromb Vasc Biol 26(8):1821-7. [PubMed: 16728652]  [MGI Ref ID J:127977]

Singaraja RR; Van Eck M; Bissada N; Zimetti F; Collins HL; Hildebrand RB; Hayden A; Brunham LR; Kang MH; Fruchart JC; Van Berkel TJ; Parks JS; Staels B; Rothblat GH; Fievet C; Hayden MR. 2006. Both hepatic and extrahepatic ABCA1 have discrete and essential functions in the maintenance of plasma high-density lipoprotein cholesterol levels in vivo. Circulation 114(12):1301-9. [PubMed: 16940190]  [MGI Ref ID J:124718]

Stukas S; May S; Wilkinson A; Chan J; Donkin J; Wellington CL. 2012. The LXR agonist GW3965 increases apoA-I protein levels in the central nervous system independent of ABCA1. Biochim Biophys Acta 1821(3):536-46. [PubMed: 21889608]  [MGI Ref ID J:182371]

Tang C; Liu Y; Kessler PS; Vaughan AM; Oram JF. 2009. The macrophage cholesterol exporter ABCA1 functions as an anti-inflammatory receptor. J Biol Chem 284(47):32336-43. [PubMed: 19783654]  [MGI Ref ID J:156325]

Taylor SR; Gonzalez-Begne M; Dewhurst S; Chimini G; Higgins CF; Melvin JE; Elliott JI. 2008. Sequential shrinkage and swelling underlie P2X7-stimulated lymphocyte phosphatidylserine exposure and death. J Immunol 180(1):300-8. [PubMed: 18097031]  [MGI Ref ID J:130933]

Terasaka N; Westerterp M; Koetsveld J; Fernandez-Hernando C; Yvan-Charvet L; Wang N; Sessa WC; Tall AR. 2010. ATP-binding cassette transporter G1 and high-density lipoprotein promote endothelial NO synthesis through a decrease in the interaction of caveolin-1 and endothelial NO synthase. Arterioscler Thromb Vasc Biol 30(11):2219-25. [PubMed: 20798376]  [MGI Ref ID J:183208]

Terasaka N; Yu S; Yvan-Charvet L; Wang N; Mzhavia N; Langlois R; Pagler T; Li R; Welch CL; Goldberg IJ; Tall AR. 2008. ABCG1 and HDL protect against endothelial dysfunction in mice fed a high-cholesterol diet. J Clin Invest 118(11):3701-13. [PubMed: 18924609]  [MGI Ref ID J:144580]

Tsujita M; Wu CA; Abe-Dohmae S; Usui S; Okazaki M; Yokoyama S. 2005. On the hepatic mechanism of HDL assembly by the ABCA1/apoA-I pathway. J Lipid Res 46(1):154-62. [PubMed: 15520446]  [MGI Ref ID J:94964]

Vrins CL; Ottenhoff R; van den Oever K; de Waart DR; Kruyt JK; Zhao Y; van Berkel TJ; Havekes LM; Aerts JM; van Eck M; Rensen PC; Groen AK. 2012. Trans-intestinal cholesterol efflux is not mediated through high density lipoprotein. J Lipid Res 53(10):2017-23. [PubMed: 22802462]  [MGI Ref ID J:188045]

Wahrle SE; Jiang H; Parsadanian M; Hartman RE; Bales KR; Paul SM; Holtzman DM. 2005. Deletion of Abca1 increases Abeta deposition in the PDAPP transgenic mouse model of Alzheimer disease. J Biol Chem 280(52):43236-42. [PubMed: 16207708]  [MGI Ref ID J:105901]

Wahrle SE; Jiang H; Parsadanian M; Kim J; Li A; Knoten A; Jain S; Hirsch-Reinshagen V; Wellington CL; Bales KR; Paul SM; Holtzman DM. 2008. Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease. J Clin Invest 118(2):671-82. [PubMed: 18202749]  [MGI Ref ID J:131400]

Wahrle SE; Jiang H; Parsadanian M; Legleiter J; Han X; Fryer JD; Kowalewski T; Holtzman DM. 2004. ABCA1 is required for normal central nervous system ApoE levels and for lipidation of astrocyte-secreted apoE. J Biol Chem 279(39):40987-93. [PubMed: 15269217]  [MGI Ref ID J:93336]

Wang X; Collins HL; Ranalletta M; Fuki IV; Billheimer JT; Rothblat GH; Tall AR; Rader DJ. 2007. Macrophage ABCA1 and ABCG1, but not SR-BI, promote macrophage reverse cholesterol transport in vivo. J Clin Invest 117(8):2216-24. [PubMed: 17657311]  [MGI Ref ID J:123959]

Westerterp M; Gourion-Arsiquaud S; Murphy AJ; Shih A; Cremers S; Levine RL; Tall AR; Yvan-Charvet L. 2012. Regulation of hematopoietic stem and progenitor cell mobilization by cholesterol efflux pathways. Cell Stem Cell 11(2):195-206. [PubMed: 22862945]  [MGI Ref ID J:190966]

Williamson P; Halleck MS; Malowitz J; Ng S; Fan X; Krahling S; Remaley AT; Schlegel RA. 2007. Transbilayer phospholipid movements in ABCA1-deficient cells. PLoS ONE 2(1):e729. [PubMed: 17710129]  [MGI Ref ID J:129392]

Yamamoto S; Tanigawa H; Li X; Komaru Y; Billheimer JT; Rader DJ. 2011. Pharmacologic suppression of hepatic ATP-binding cassette transporter 1 activity in mice reduces high-density lipoprotein cholesterol levels but promotes reverse cholesterol transport. Circulation 124(12):1382-90. [PubMed: 21859969]  [MGI Ref ID J:189383]

Yvan-Charvet L; Pagler T; Gautier EL; Avagyan S; Siry RL; Han S; Welch CL; Wang N; Randolph GJ; Snoeck HW; Tall AR. 2010. ATP-binding cassette transporters and HDL suppress hematopoietic stem cell proliferation. Science 328(5986):1689-93. [PubMed: 20488992]  [MGI Ref ID J:161310]

Yvan-Charvet L; Pagler TA; Seimon TA; Thorp E; Welch CL; Witztum JL; Tabas I; Tall AR. 2010. ABCA1 and ABCG1 protect against oxidative stress-induced macrophage apoptosis during efferocytosis. Circ Res 106(12):1861-9. [PubMed: 20431058]  [MGI Ref ID J:175055]

Yvan-Charvet L; Ranalletta M; Wang N; Han S; Terasaka N; Li R; Welch C; Tall AR. 2007. Combined deficiency of ABCA1 and ABCG1 promotes foam cell accumulation and accelerates atherosclerosis in mice. J Clin Invest 117(12):3900-8. [PubMed: 17992262]  [MGI Ref ID J:130777]

Yvan-Charvet L; Welch C; Pagler TA; Ranalletta M; Lamkanfi M; Han S; Ishibashi M; Li R; Wang N; Tall AR. 2008. Increased inflammatory gene expression in ABC transporter-deficient macrophages: free cholesterol accumulation, increased signaling via toll-like receptors, and neutrophil infiltration of atherosclerotic lesions. Circulation 118(18):1837-47. [PubMed: 18852364]  [MGI Ref ID J:165622]

Zhao Y; Pennings M; Hildebrand RB; Ye D; Calpe-Berdiel L; Out R; Kjerrulf M; Hurt-Camejo E; Groen AK; Hoekstra M; Jessup W; Chimini G; Van Berkel TJ; Van Eck M. 2010. Enhanced foam cell formation, atherosclerotic lesion development, and inflammation by combined deletion of ABCA1 and SR-BI in Bone marrow-derived cells in LDL receptor knockout mice on western-type diet. Circ Res 107(12):e20-31. [PubMed: 21071707]  [MGI Ref ID J:178508]

van der Veen JN; Kruit JK; Havinga R; Baller JF; Chimini G; Lestavel S; Staels B; Groot PH; Groen AK; Kuipers F. 2005. Reduced cholesterol absorption upon PPAR{delta} activation coincides with decreased intestinal expression of NPC1L1. J Lipid Res 46(3):526-34. [PubMed: 15604518]  [MGI Ref ID J:96527]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryChimeric animals were mated with DBA/1J mice for an undetermined number of generations. Upon arrival at The Jackson Laboratory, mice were rederived and while held in a live colony, were maintained as heterozygotes by matings with wildtype DBA/1LacJ. Coat color expected from breeding:Dilute Brown

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   001140 DBA/1LacJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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