Strain Name: |
B6;129S-Mttptm2Sgy/J |
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Stock Number: |
003902 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Mttp; Mttptm2Sgy; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Stephen Young, Gladstone Institutes UCSF Generation N1F6+2N1p Strain Description
These mice possess loxP sites located 2.5 kb 5' of exon 1 and flanking a neomycin resistance gene inserted into intron 1 of the Mttp gene. Mice that are homozygous for this floxed Mttp allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
When bred to a strain expressing interferon inducible Cre recombinase in liver tissue (see Stock No. 003556 for example), this mutant mouse strain may be useful in lipoprotein research.Strain Development
A targeting vector containing Mttp sequence, neomycin resistance and herpes simplex virus thymidine kinase genes was utilized in the construction of this mutant. Similarly oriented loxP sites were placed 2.5 kb upstream of exon 1 and flanking the neomycin resistance gene (located in intron 1). The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Mice harboring the mutant allele were obtained and bred to homozygosity.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Mttptm2Sgy | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Steven G Young | ||
| Mutation Made By | Stephen Young, Gladstone Institutes UCSF | ||
| Strain of Origin | 129S4/SvJae | ||
| Site of Expression | cre mutation deletes floxed Mttp gene, reversing hypercholesterolemia phenotype | ||
| Gene Symbol and Name | Mttp, microsomal triglyceride transfer protein | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | ABL; MGC149819; MGC149820; MTP; | ||
| Molecular Note | A neomycin resistance cassette flanked by loxP sites was inserted into intron 1. An additional loxP site was inserted 2.5 kb upstream of the promoter and exon 1. [MGI Ref ID J:54612] | ||
Colony Maintenance
| Breeding & Husbandry | This strain originated and is maintained as a homozygote on a B6;129S background. |
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Related Strains
Strains carrying Mttptm2Sgy allele
004192 STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J View Strains carrying Mttptm2Sgy (1 strain)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
Mttptm2Sgy relatedResearch Tools
Cre-lox System (loxP-flanked Sequences)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Metabolism Research
Lipid Metabolism
References
Selected Reference(s)
Additional ReferencesRaabe M; Veniant MM; Sullivan MA; Zlot CH; Bjorkegren J; Nielsen LB; Wong JS; Hamilton RL; Young SG. 1999. Analysis of the role of microsomal triglyceride transfer protein in the liver of tissue-specific knockout mice. J Clin Invest 103(9):1287-98. [PubMed: 10225972] [MGI Ref ID J:54612]
Price and Supply Information
| Strain Name: | B6;129S-Mttptm2Sgy/J |
| Stock Number: | 003902 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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