Description

Strain Information

Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote         (Female x Male)   01-MAR-06 Species laboratory mouse Generation N10F?+F1N18 (26-JAN-09) Generation Definitions   Donating Investigator Xian-cheng Jiang,   Columbia University

Description
Mice carrying the human CETP minigene under the control of its own promoter show a marked increase in CETP mRNA in liver when mice are fed a high fat, high cholesterol diet. Less dramatic increases are observed in spleen, small intestine and adipose tissues. Elevated plasma CETP protein levels are also observed. Mice carrying human CETP show reduced levels of plasma high density lipoprotein. This strain has been a useful model in studies related to cholesterol metabolism.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of CETP

001929

C57BL/6-Tg(CETP)UCTP20Pnu/J

View Strains carrying other alleles of CETP     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(CETP)5203Tall/0

        involves: C57BL/6 * CBA/J

• homeostasis/metabolism phenotype
• abnormal lipid level
  • hepatic cholesteryl ester level is lower than in Apoc1-defcient mice   (MGI Ref ID J:117482)
• abnormal phospholipid level
  • biliary level is decreased compared to Apoc1-deficient mice   (MGI Ref ID J:117482)
• decreased cholesterol level
  • biliary level is decreased compared to Apoc1-deficient mice   (MGI Ref ID J:117482)
• decreased liver triglyceride level
  • hepatic levels are significantly lower than in Apoc1-deficient mice   (MGI Ref ID J:117482)
• liver/biliary system phenotype
• decreased liver triglyceride level
  • hepatic levels are significantly lower than in Apoc1-deficient mice   (MGI Ref ID J:117482)

Tg(CETP)5203Tall/0

        involves: C57BL/6J * CBA/J

• homeostasis/metabolism phenotype
• *normal* homeostasis/metabolism phenotype
  • despite being fed an atherogenic diet, mice exhibit no change in total cholesterol, triglyceride and HDL-cholesterol levels compared to similarly treated wild-type mice   (MGI Ref ID J:128254)
• decreased circulating HDL cholesterol level
  • compared to in Apobec1^tm1Chan homozygotes and wild-type mice   (MGI Ref ID J:48202)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(CETP)5203Tall
Allele Name		transgene insertion 5203, Alan Tall
Allele Type		Transgenic (random, expressed)
Common Name(s)		CETP; NFR-CETP;
Mutation Made By		Xian-cheng Jiang,   Columbia University
Strain of Origin		(C57BL/6J x CBA/J)F1
Expressed Gene		CETP, cholesteryl ester transfer protein, plasma, human
Promoter		CETP, cholesteryl ester transfer protein, plasma, human
General Note		Transgenic mice on a genetic background that involves C57BL/6J and CBA/J show a marked increase in CETP mRNA in liver when mice are fed a high fat, high cholesterol diet. Less dramatic increases are observed in spleen, small intestine and adipose tissues. Elevated plasma CETP protein levels are also observed.
Molecular Note		The transgene contains the human CETP mini gene under the control of its own promoter. The mini gene consists of 3.2 Kb of 5' flanking sequence, exons 1-2 and 13-16, and 2.0 Kb of 3' flanking sequence. [MGI Ref ID J:93800]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(CETP), Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Jiang XC; Agellon LB; Walsh A; Breslow JL; Tall A. 1992. Dietary cholesterol increases transcription of the human cholesteryl ester transfer protein gene in transgenic mice. Dependence on natural flanking sequences. J Clin Invest 90(4):1290-5. [PubMed: 1401066]  [MGI Ref ID J:69181]

Additional References

Masucci-Magoulas L; Moulin P; Jiang XC; Richardson H; Walsh A; Breslow JL; Tall A. 1995. Decreased cholesteryl ester transfer protein (CETP) mRNA and protein and increased high density lipoprotein following lipopolysaccharide administration in human CETP transgenic mice. J Clin Invest 95(4):1587-94. [PubMed: 7706465]  [MGI Ref ID J:93801]

Tg(CETP)5203Tall related

Agellon LB; Walsh A; Hayek T; Moulin P; Jiang XC; Shelanski SA; Breslow JL; Tall AR. 1991. Reduced high density lipoprotein cholesterol in human cholesteryl ester transfer protein transgenic mice. J Biol Chem 266(17):10796-801. [PubMed: 2040599]  [MGI Ref ID J:93800]

Berti JA; Amaral ME; Boschero AC; Nunes VS; Harada LM; Castilho LN; Oliveira HC. 2001. Thyroid hormone increases plasma cholesteryl ester transfer protein activity and plasma high-density lipoprotein removal rate in transgenic mice. Metabolism 50(5):530-6. [PubMed: 11319713]  [MGI Ref ID J:69090]

Berti JA; de Faria EC; Oliveira HC. 2005. Atherosclerosis in aged mice over-expressing the reverse cholesterol transport genes. Braz J Med Biol Res 38(3):391-8. [PubMed: 15761619]  [MGI Ref ID J:128254]

Casquero AC; Berti JA; Salerno AG; Bighetti EJ; Cazita PM; Ketelhuth DF; Gidlund M; Oliveira HC. 2006. Atherosclerosis is enhanced by testosterone deficiency and attenuated by CETP expression in transgenic mice. J Lipid Res 47(7):1526-34. [PubMed: 16603720]  [MGI Ref ID J:112057]

Cazita PM; Berti JA; Aoki C; Gidlund M; Harada LM; Nunes VS; Quintao EC; Oliveira HC. 2003. Cholesteryl ester transfer protein expression attenuates atherosclerosis in ovariectomized mice. J Lipid Res 44(1):33-40. [PubMed: 12518020]  [MGI Ref ID J:120685]

Cheema SK; Agarwal-Mawal A; Murray CM; Tucker S. 2005. Lack of stimulation of cholesteryl ester transfer protein by cholesterol in the presence of a high-fat diet. J Lipid Res 46(11):2356-66. [PubMed: 16106052]  [MGI Ref ID J:104777]

Cheema SK; Rashid-Kolvear F. 2003. Streptozotocin-induced increase in cholesterol ester transfer protein (CETP) and its reversal by insulin in transgenic mice expressing human CETP. Can J Physiol Pharmacol 81(10):997-1004. [PubMed: 14608418]  [MGI Ref ID J:136412]

Francone OL; Royer L; Haghpassand M. 1996. Increased prebeta-HDL levels, cholesterol efflux, and LCAT-mediated esterification in mice expressing the human cholesteryl ester transfer protein (CETP) and human apolipoprotein A-I (apoA-I) transgenes. J Lipid Res 37(6):1268-77. [PubMed: 8808761]  [MGI Ref ID J:111816]

Gautier T; Masson D; Jong MC; Pais de Barros JP; Duverneuil L; Le Guern N; Deckert V; Dumont L; Bataille A; Zak Z; Jiang XC; Havekes LM; Lagrost L. 2005. Apolipoprotein CI overexpression is not a relevant strategy to block cholesteryl ester transfer protein (CETP) activity in CETP transgenic mice. Biochem J 385(Pt 1):189-95. [PubMed: 15339254]  [MGI Ref ID J:117536]

Gautier T; Tietge UJ; Boverhof R; Perton FG; Le Guern N; Masson D; Rensen PC; Havekes LM; Lagrost L; Kuipers F. 2007. Hepatic lipid accumulation in apolipoprotein C-I-deficient mice is potentiated by cholesteryl ester transfer protein. J Lipid Res 48(1):30-40. [PubMed: 17053273]  [MGI Ref ID J:117482]

Hayek T; Chajek-Shaul T; Walsh A; Agellon LB; Moulin P; Tall AR; Breslow JL. 1992. An interaction between the human cholesteryl ester transfer protein (CETP) and apolipoprotein A-I genes in transgenic mice results in a profound CETP-mediated depression of high density lipoprotein cholesterol levels. J Clin Invest 90(2):505-10. [PubMed: 1644921]  [MGI Ref ID J:111567]

Hernandez M; Wright SD; Cai TQ. 2007. Critical role of cholesterol ester transfer protein in nicotinic acid-mediated HDL elevation in mice. Biochem Biophys Res Commun 355(4):1075-80. [PubMed: 17335774]  [MGI Ref ID J:120331]

Hime NJ; Black AS; Bulgrien JJ; Curtiss LK. 2008. Leukocyte-derived hepatic lipase increases HDL and decreases en face aortic atherosclerosis in LDLr-/- mice expressing CETP. J Lipid Res 49(10):2113-23. [PubMed: 18599739]  [MGI Ref ID J:140426]

Hoekstra M; Ye D; Hildebrand RB; Zhao Y; Lammers B; Stitzinger M; Kuiper J; Van Berkel TJ; Van Eck M. 2009. Scavenger receptor class B type I-mediated uptake of serum cholesterol is essential for optimal adrenal glucocorticoid production. J Lipid Res 50(6):1039-46. [PubMed: 19179307]  [MGI Ref ID J:149859]

Jiang XC; Tall AR; Qin S; Lin M; Schneider M; Lalanne F; Deckert V; Desrumaux C; Athias A; Witztum JL; Lagrost L. 2002. Phospholipid transfer protein deficiency protects circulating lipoproteins from oxidation due to the enhanced accumulation of vitamin E. J Biol Chem 277(35):31850-6. [PubMed: 12105225]  [MGI Ref ID J:120468]

Kawano K; Qin SC; Lin M; Tall AR; Jiang Xc. 2000. Cholesteryl ester transfer protein and phospholipid transfer protein have nonoverlapping functions in vivo J Biol Chem 275(38):29477-81. [PubMed: 10893412]  [MGI Ref ID J:64848]

Lie J; Moerland M; van Gent T; van Haperen R; Scheek L; Sadeghi-Niaraki F; de Crom R; van Tol A. 2006. Sex differences in atherosclerosis in mice with elevated phospholipid transfer protein activity are related to decreased plasma high density lipoproteins and not to increased production of triglycerides. Biochim Biophys Acta 1761(9):1070-7. [PubMed: 16935026]  [MGI Ref ID J:116584]

MacLean PS; Bower JF; Vadlamudi S; Osborne JN; Bradfield JF; Burden HW; Bensch WH; Kauffman RF; Barakat HA. 2003. Cholesteryl ester transfer protein expression prevents diet-induced atherosclerotic lesions in male db/db mice. Arterioscler Thromb Vasc Biol 23(8):1412-5. [PubMed: 12791674]  [MGI Ref ID J:109846]

Masucci-Magoulas L; Goldberg IJ; Bisgaier CL; Serajuddin H; Francone OL ; Breslow JL ; Tall AR. 1997. A mouse model with features of familial combined hyperlipidemia. Science 275(5298):391-4. [PubMed: 8994037]  [MGI Ref ID J:37861]

Masucci-Magoulas L; Moulin P; Jiang XC; Richardson H; Walsh A; Breslow JL; Tall A. 1995. Decreased cholesteryl ester transfer protein (CETP) mRNA and protein and increased high density lipoprotein following lipopolysaccharide administration in human CETP transgenic mice. J Clin Invest 95(4):1587-94. [PubMed: 7706465]  [MGI Ref ID J:93801]

Masucci-Magoulas L; Plump A; Jiang XC; Walsh A; Breslow JL; Tall AR. 1996. Profound induction of hepatic cholesteryl ester transfer protein transgene expression in apolipoprotein E and low density lipoprotein receptor gene knockout mice. A novel mechanism signals changes in plasma cholesterol levels. J Clin Invest 97(1):154-61. [PubMed: 8550828]  [MGI Ref ID J:111394]

Nakamuta M; Taniguchi S; Ishida BY; Kobayashi K; Chan L. 1998. Phenotype interaction of apobec-1 and CETP, LDLR, and apoE gene expression in mice: role of apoB mRNA editing in lipoprotein phenotype expression. Arterioscler Thromb Vasc Biol 18(5):747-55. [PubMed: 9598833]  [MGI Ref ID J:48202]

Plump AS; Azrolan N; Odaka H; Wu L; Jiang X; Tall A; Eisenberg S ; Breslow JL. 1997. ApoA-I knockout mice: characterization of HDL metabolism in homozygotes and identification of a post-RNA mechanism of apoA-I up-regulation in heterozygotes. J Lipid Res 38(5):1033-47. [PubMed: 9186920]  [MGI Ref ID J:40819]

Plump AS; Masucci-Magoulas L; Bruce C; Bisgaier CL; Breslow JL; Tall AR. 1999. Increased atherosclerosis in ApoE and LDL receptor gene knock-out mice as a result of human cholesteryl ester transfer protein transgene expression. Arterioscler Thromb Vasc Biol 19(4):1105-10. [PubMed: 10195942]  [MGI Ref ID J:55980]

Salerno AG; Patricio PR; Berti JA; Oliveira HC. 2009. Cholesteryl ester transfer protein (CETP) increases postprandial triglyceridaemia and delays triacylglycerol plasma clearance in transgenic mice. Biochem J 419(3):629-34. [PubMed: 19191759]  [MGI Ref ID J:149787]

Salerno AG; Silva TR; Amaral ME; Alberici LC; Bonfleur ML; Patricio PR; Francesconi EP; Grassi-Kassisse DM; Vercesi AE; Boschero AC; Oliveira HC. 2007. Overexpression of apolipoprotein CIII increases and CETP reverses diet-induced obesity in transgenic mice. Int J Obes (Lond) 31(10):1586-95. [PubMed: 17471296]  [MGI Ref ID J:151293]

Van Eck M; Ye D; Hildebrand RB; Kar Kruijt J; de Haan W; Hoekstra M; Rensen PC; Ehnholm C; Jauhiainen M; Van Berkel TJ. 2007. Important role for bone marrow-derived cholesteryl ester transfer protein in lipoprotein cholesterol redistribution and atherosclerotic lesion development in LDL receptor knockout mice. Circ Res 100(5):678-85. [PubMed: 17293475]  [MGI Ref ID J:133704]

Westerterp M; van der Hoogt CC; de Haan W; Offerman EH; Dallinga-Thie GM; Jukema JW; Havekes LM; Rensen PC. 2006. Cholesteryl ester transfer protein decreases high-density lipoprotein and severely aggravates atherosclerosis in APOE*3-Leiden mice. Arterioscler Thromb Vasc Biol 26(11):2552-9. [PubMed: 16946130]  [MGI Ref ID J:128047]

Zhou H; Li Z; Silver DL; Jiang XC. 2006. Cholesteryl ester transfer protein (CETP) expression enhances HDL cholesteryl ester liver delivery, which is independent of scavenger receptor BI, LDL receptor related protein and possibly LDL receptor. Biochim Biophys Acta 1761(12):1482-8. [PubMed: 17055779]  [MGI Ref ID J:118151]

de Haan W; de Vries-van der Weij J; Mol IM; Hoekstra M; Romijn JA; Jukema JW; Havekes LM; Princen HM; Rensen PC. 2009. PXR agonism decreases plasma HDL levels in ApoE3-Leiden.CETP mice. Biochim Biophys Acta 1791(3):191-7. [PubMed: 19150509]  [MGI Ref ID J:148609]

van der Hoogt CC; de Haan W; Westerterp M; Hoekstra M; Dallinga-Thie GM; Romijn JA; Princen HM; Jukema JW; Havekes LM; Rensen PC. 2007. Fenofibrate increases HDL-cholesterol by reducing cholesteryl ester transfer protein expression. J Lipid Res 48(8):1763-71. [PubMed: 17525476]  [MGI Ref ID J:123774]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	This strain arose on a (C57BL/6J x CBA/J)F1 background and has been backcrossed to C57BL/6 for at least ten generations (1/01)Coat color expected from breeding:Black
Mating System	Inbred x Hemizygote         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.
• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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