Strain Name: |
STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J |
|---|---|
Stock Number: |
003919 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
STOCK Tg(ACTB-Bgeo/ALPP)1Lbe/J (Changed: 08-MAY-08
) |
| Genes & Alleles | ALPP; Actb; Bgeo; Tg(CAG-Bgeo/ALPP)1Lbe; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Corrinne Lobe, Sunnybrook & Women's College HSC Generation N11F2pN1 (24-JUL-05) Strain Description
These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being erythrocytes, chondrocytes and adipocytes. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess cre excision activity at the individual cell level.Strain Development
A transgenic construct containing the CMV enhancer/chicken beta-actin promoter, a loxP-flanked Bgeo reporter/SV40 polyadenylation signal segment, and the human placental alkaline phosphatase gene followed by a rabbit beta-globin polyadenylation sequence, was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells expressing lacZ were selected and utilized in ES cell/diploid embryo aggregation in conjunction with CD1 outbred embryos resulting in chimeric animals.
Gene & Allele Details
| Allele Symbol | Tg(CAG-Bgeo/ALPP)1Lbe | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Corrinne Lobe | ||
| Common Name(s) | Tg(ACTB-Bgeo/ALPP)1Lbe; Z/AP; ZAP; | ||
| Mutation Made By | Corrinne Lobe, Sunnybrook & Women's College HSC | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being erythrocytes, chondrocytes and adipocytes; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with alkaline phosphatase expression | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental (Regan isozyme), human | ||
| Promoter | Actb, actin, beta, cytoplasmic, mouse, laboratory | ||
| General Note | Cre excision of lacZ and the neomycin resistance gene results in expression of the ALPP reporter instead. | ||
| Molecular Note | The transgene contains the CMV enhancer/chicken beta-actin promoter, a floxed Beta-geo reporter/SV40 polyadenylation signal segment, and the human placental alkaline phosphatase gene followed by a rabbit Hbb polyadenylation sequence. R1 embryonic stem (ES) cells were used to introduce the transgene into the mouse genome. In transgenic mice expression of lacZ is widespread, with notable exceptions being erythrocytes, chondrocytes and adipocytes. This expression is observed throughout all embryonic and adult stages. [J:93700] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(ACTB-Bgeo/ALPP)1Lbe
Colony Maintenance
| Breeding & Husbandry | This strain arose on the 129X1/SvJ x 129S1/Sv (R1 ES cell line) background and was maintained by the donating investigator as a hemizygote by crosses to wildtype outbred CD1 mice. It is unknown if homozygotes are viable. Coat color expected from breeding:Agouti |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
007066 B6.SJL-Tg(Crx-GFP,-ALPP)1Clc/J 006882 STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J 006613 STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J 005105 STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
Bgeo relatedResearch Tools
Cre-lox-System (loxP-flanked Sequences: Test/Reporter)
Genetics Research (Tissue/Cell Markers: Cre-lox system)
Genetics Research (Tissue/Cell Markers: multiple)
lacZ Expression
Research Tools
lacZ Expression
References
Selected Reference(s)
Additional ReferencesLobe CG; Koop KE; Kreppner W; Lomeli H; Gertsenstein M; Nagy A. 1999. Z/AP, a double reporter for cre-mediated recombination. Dev Biol 208(2):281-92. [PubMed: 10191045] [J:93700]
Price and Supply Information
| Strain Name: | STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J |
| Stock Number: | 003919 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at the Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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