Strain Name: |
C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ |
|---|---|
Stock Number: |
003927 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
C57BL/6J-Tg(Sry-EGFP)92Ei (Changed: 11-APR-07
) |
| Genes & Alleles | GFP; Sry; Tg(Sry-EGFP)92Ei; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Eva Eicher, The Jackson Laboratory Generation F23+F1 (23-NOV-07) Strain Description
Tg(Sry-EGFP)92Ei has been shown to drive expression of GFP in pre-Sertoli and pre-granulosa cells of the genital ridge, with expression stronger in homozygotes than hemizygotes. Gonadal expression is not found in germ cells or vascular endothelial cells, but is restricted to a subset of somatic cells. Expression in the genital ridge begins in the center and moves out to the poles, with a bias toward the caudal pole. GFP expression is also found in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males, but not females, with lower levels of expression observed sparsely throughout the cortex. Strongest expression in the male brain is in the pars compacta and, at the cellular level, both cytoplasmic and nuclear localization are found.Strain Development
This transgene has a 7.7 kb Sry promoter driving expression of enhanced green fluorescing protein. Tg(Sry-EGFP)92Ei was generated by microinjection into the pronucleus of C57BL/6J fertilized eggs and the strain has been maintained on this background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Sry-EGFP)92Ei | ||
|---|---|---|---|
| Allele Name | transgene insertion 92, Eva M Eicher | ||
| Common Name(s) | Tg92Ei; | ||
| Strain of Origin | C57BL/6J | ||
| Site of Expression | Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Sry, sex determining region of Chr Y, mouse, laboratory | ||
| Molecular Note | The transgenic vector was composed of the Sry promoter region from bp 542-8304 inserted upstream of the EGFP-1 coding region. The construct contains two SV40 early mRNA polyadenylation signal in place of the endogenous Sry 3' UTR. Expression of EGFP indicates that the spatial expression of EGFP mRNA and protein is similar to endogenous Sry . [MGI Ref ID J:73280] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Tg(GFP) QPCR
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:Tg(Sry-EGFP)92Ei related
Developmental Biology Research
Neurobiology Research
Research Tools
Fluorescent Proteins
Reproductive Biology Research
References
Selected Reference(s)
Albrecht KH; Eicher EM. 2001. Evidence that sry is expressed in pre-sertoli cells and sertoli and granulosa cells have a common precursor. Dev Biol 240(1):92-107. [PubMed: 11784049] [MGI Ref ID J:73280]
Dewing P; Chiang CW; Sinchak K; Sim H; Fernagut PO; Kelly S; Chesselet MF; Micevych PE; Albrecht KH; Harley VR; Vilain E. 2006. Direct regulation of adult brain function by the male-specific factor SRY. Curr Biol 16(4):415-20. [PubMed: 16488877] [MGI Ref ID J:123875]
Price and Supply Information
| Strain Name: | C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ |
| Stock Number: | 003927 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $203.80 | Homozygous for Tg(Sry-EGFP)92Ei | |||
| Pair | $407.60 | Homozygous for Tg(Sry-EGFP)92Ei x Homozygous for Tg(Sry-EGFP)92Ei | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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