Strain Name: |
129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J |
|---|---|
Stock Number: |
003946 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Gt(ROSA)26Sor; Gt(ROSA)26Sortm1(FLP1)Dym; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Susan Dymecki, Harvard Medical School Generation ?+F16 (06-DEC-07) Strain Description
This knock-in strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the Gt(ROSA)26Sor promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wild type FLP at 37C and 40C, respectively. Homozygous mice for the knock-in allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Gt(ROSA)26Sor promoter drives expression in a constitutive fashion from preimplantation onward. Extensive target gene recombination can be achieved in most tissue types, including cells of the developing germ line. This mutant strain is suitable for use as a deleter strain and serves as a source of FLP1-expressing primary cell lines.Strain Development
A knock-in construct (R26Fki) was built by inserting a FLPe cassette into a ROSA26 knock-in vector. This construct includes a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter. The construct was electroporated into 129S4/S4JaeSor-derived AK7 embryonic stem (ES) cells, thereby disrupting the Gt(ROSA)26Sor locus. Correctly targeted ES cells were utilized to generate chimeras.
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sortm1(FLP1)Dym | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Susan Dymecki | ||
| Common Name(s) | CAGGS-Flpe; FLP; FLP deleter; FLPe; FLPeR; R26Fki; ROSA26::FLPe knock in; flipper; | ||
| Mutation Made By | Susan Dymecki, Harvard Medical School | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | constitutive expression of FLP1 recombinase from preimplantation onward and in most tissue types (including cells of the developing germ line) | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | An enhanced flp cassette containing a splice acceptor site was ligated to a downstream PGK-neo cassette and inserted at the endogenous locus. Efficient frt-specific recombination in transgenic mice carrying a reporter gene was reported to reflect constituitive expression of the Gt(ROSA)26Sor from preimplantation onward. [MGI Ref ID J:66893] [MGI Ref ID J:92442] | ||
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gt(ROSA)26Sortm1(FLP1)Dym
Colony Maintenance
| Breeding & Husbandry | This strain originated and is mainatained on a 129S4/SvJaeSor background. Homozygotes are viable. Coat color expected from breeding:Agouti |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (37 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
FLP-FRT System (FLP Recombinase Expression: Germline/Embryonic Expression)
Genetics Research (Mutagenesis and Transgenesis: FLP recombinase)
References
Selected Reference(s)
Additional ReferencesFarley FW; Soriano P; Steffen LS; Dymecki SM. 2000. Widespread recombinase expression using FLPeR (Flipper) mice Genesis 28(3-4):106-10. [PubMed: 11105051] [MGI Ref ID J:66893]
Price and Supply Information
| Strain Name: | 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J |
| Stock Number: | 003946 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form