| |||||||||||||||
| FLPeR mice harbor the FLPe knock-in allele with widespread expression of the FLPe variant of the Saccharomyces cerevisiae FLP1 recombinase gene driven by the Gt(ROSA)26Sor promoter. When bred with mice containing a frt-flanked sequence of interest, FLP-mediated recombination will result in deletion of the frt-flanked sequence(s) in the offspring. This FLP deleter strain may be useful for generating frt-FLP conditional mutations and serves as a source of FLP1-expressing primary cell lines. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation ?+F18 (19-NOV-08) Donating Investigator Susan Dymecki, Harvard Medical School Description
Homozygous FLPeR mice are viable and fertile, with widespread expression of the FLPe variant of the Saccharomyces cerevisiae FLP1 recombinase gene driven by the Gt(ROSA)26Sor promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wild type FLP at 37C and 40C, respectively. The Gt(ROSA)26Sor promoter drives expression from preimplantation onward and extensive target gene recombination can be achieved in most tissue types, including cells of the developing germ line. When bred with mice containing a frt-flanked sequence of interest, FLP-mediated recombination will result in deletion of the frt-flanked sequence(s) in the offspring. This FLP deleter strain may be useful for generating frt-FLP conditional mutations and serves as a source of FLP1-expressing primary cell lines.Development
A knock-in construct (R26Fki) was built by inserting a FLPe cassette into a ROSA26 knock-in vector. This construct includes a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter. The construct was electroporated into 129S4/S4JaeSor-derived AK7 embryonic stem (ES) cells, thereby disrupting the Gt(ROSA)26Sor locus. Correctly targeted ES cells were utilized to generate chimeras.
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm1(FLP1)Dym allele
009086 B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ View Strains carrying Gt(ROSA)26Sortm1(FLP1)Dym (1 strain)
Additional Web Information
FLP-FRT Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
FLP-FRT System
FLP Recombinase Expression: Germline/Embryonic Expression
Genetics Research
Mutagenesis and Transgenesis: FLP recombinase
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(FLP1)Dym | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Susan Dymecki | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | CAGGS-Flpe; FLP; FLP deleter; FLPe; FLPeR; R26Fki; ROSA26::FLPe knock in; flipper; | ||
| Mutation Made By | Susan Dymecki, Harvard Medical School | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | constitutive expression of FLP1 recombinase from preimplantation onward and in most tissue types (including cells of the developing germ line) | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Driver Note | Gt(ROSA)26Sor | ||
| Molecular Note | An enhanced flp cassette containing a splice acceptor site was ligated to a downstream PGK-neo cassette and inserted at the endogenous locus. Efficient frt-specific recombination in transgenic mice carrying a reporter gene was reported to reflect constituitive expression of the Gt(ROSA)26Sor from preimplantation onward. [MGI Ref ID J:66893] [MGI Ref ID J:92442] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(FLP1)Dym, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Farley FW; Soriano P; Steffen LS; Dymecki SM. 2000. Widespread recombinase expression using FLPeR (Flipper) mice Genesis 28(3-4):106-10. [PubMed: 11105051] [MGI Ref ID J:66893]
Additional References
Gt(ROSA)26Sortm1(FLP1)Dym relatedAffar el B; Gay F; Shi Y; Liu H; Huarte M; Wu S; Collins T; Li E; Shi Y. 2006. Essential dosage-dependent functions of the transcription factor yin yang 1 in late embryonic development and cell cycle progression. Mol Cell Biol 26(9):3565-81. [PubMed: 16611997] [MGI Ref ID J:108369]
Amleh A; Nair SJ; Sun J; Sutherland A; Hasty P; Li R. 2009. Mouse cofactor of BRCA1 (Cobra1) is required for early embryogenesis. PLoS ONE 4(4):e5034. [PubMed: 19340312] [MGI Ref ID J:148508]
Anderson MP; Mochizuki T; Xie J; Fischler W; Manger JP; Talley EM; Scammell TE; Tonegawa S. 2005. Thalamic Cav3.1 T-type Ca2+ channel plays a crucial role in stabilizing sleep. Proc Natl Acad Sci U S A 102(5):1743-8. [PubMed: 15677322] [MGI Ref ID J:96099]
Astapova I; Lee LJ; Morales C; Tauber S; Bilban M; Hollenberg AN. 2008. The nuclear corepressor, NCoR, regulates thyroid hormone action in vivo. Proc Natl Acad Sci U S A 105(49):19544-9. [PubMed: 19052228] [MGI Ref ID J:142045]
Awatramani R; Soriano P; Mai JJ; Dymecki S. 2001. An Flp indicator mouse expressing alkaline phosphatase from the ROSA26 locus. Nat Genet 29(3):257-9. [PubMed: 11687793] [MGI Ref ID J:72406]
Awatramani R; Soriano P; Rodriguez C; Mai JJ; Dymecki SM. 2003. Cryptic boundaries in roof plate and choroid plexus identified by intersectional gene activation. Nat Genet 35(1):70-5. [PubMed: 12923530] [MGI Ref ID J:128980]
Backman CM; Malik N; Zhang Y; Shan L; Grinberg A; Hoffer BJ; Westphal H; Tomac AC. 2006. Characterization of a mouse strain expressing Cre recombinase from the 3' untranslated region of the dopamine transporter locus. Genesis 44(8):383-90. [PubMed: 16865686] [MGI Ref ID J:114466]
Bush JO; Soriano P. 2009. Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism. Genes Dev 23(13):1586-99. [PubMed: 19515977] [MGI Ref ID J:150043]
Chen H; McCaffery JM; Chan DC. 2007. Mitochondrial fusion protects against neurodegeneration in the cerebellum. Cell 130(3):548-62. [PubMed: 17693261] [MGI Ref ID J:132329]
Chen WV; Delrow J; Corrin PD; Frazier JP; Soriano P. 2004. Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis. Nat Genet 36(3):304-12. [PubMed: 14981515] [MGI Ref ID J:95727]
Dakic A; Metcalf D; Di Rago L; Mifsud S; Wu L; Nutt SL. 2005. PU.1 regulates the commitment of adult hematopoietic progenitors and restricts granulopoiesis. J Exp Med 201(9):1487-502. [PubMed: 15867096] [MGI Ref ID J:98188]
Dong Y; Du X; Ye J; Han M; Xu T; Zhuang Y; Tao W. 2009. A cell-intrinsic role for Mst1 in regulating thymocyte egress. J Immunol 183(6):3865-72. [PubMed: 19692642] [MGI Ref ID J:152323]
Dzhagalov I; St John A; He YW. 2007. The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages. Blood 109(4):1620-6. [PubMed: 17062731] [MGI Ref ID J:137502]
Emery B; Agalliu D; Cahoy JD; Watkins TA; Dugas JC; Mulinyawe SB; Ibrahim A; Ligon KL; Rowitch DH; Barres BA. 2009. Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination. Cell 138(1):172-85. [PubMed: 19596243] [MGI Ref ID J:150563]
Essalmani R; Zaid A; Marcinkiewicz J; Chamberland A; Pasquato A; Seidah NG; Prat A. 2008. In vivo functions of the proprotein convertase PC5/6 during mouse development: Gdf11 is a likely substrate. Proc Natl Acad Sci U S A 105(15):5750-5. [PubMed: 18378898] [MGI Ref ID J:134264]
Fan Y; Menon RK; Cohen P; Hwang D; Clemens T; DiGirolamo DJ; Kopchick JJ; Le Roith D; Trucco M; Sperling MA. 2009. Liver-specific deletion of the growth hormone receptor reveals essential role of growth hormone signaling in hepatic lipid metabolism. J Biol Chem 284(30):19937-44. [PubMed: 19460757] [MGI Ref ID J:152627]
Fan Y; Rudert WA; Grupillo M; He J; Sisino G; Trucco M. 2009. Thymus-specific deletion of insulin induces autoimmune diabetes. EMBO J 28(18):2812-24. [PubMed: 19680229] [MGI Ref ID J:152798]
Farago AF; Awatramani RB; Dymecki SM. 2006. Assembly of the brainstem cochlear nuclear complex is revealed by intersectional and subtractive genetic fate maps. Neuron 50(2):205-18. [PubMed: 16630833] [MGI Ref ID J:133039]
Feng JQ; Scott G; Guo D; Jiang B; Harris M; Ward T; Ray M; Bonewald LF; Harris SE; Mishina Y. 2008. Generation of a conditional null allele for Dmp1 in mouse. Genesis 46(2):87-91. [PubMed: 18257058] [MGI Ref ID J:135314]
Foulkes T; Nassar MA; Lane T; Matthews EA; Baker MD; Gerke V; Okuse K; Dickenson AH; Wood JN. 2006. Deletion of annexin 2 light chain p11 in nociceptors causes deficits in somatosensory coding and pain behavior. J Neurosci 26(41):10499-507. [PubMed: 17035534] [MGI Ref ID J:113256]
Grier JD; Xiong S; Elizondo-Fraire AC; Parant JM; Lozano G. 2006. Tissue-Specific Differences of p53 Inhibition by Mdm2 and Mdm4. Mol Cell Biol 26(1):192-8. [PubMed: 16354690] [MGI Ref ID J:104114]
Hertveldt V; Louryan S; van Reeth T; Dreze P; van Vooren P; Szpirer J; Szpirer C. 2008. The development of several organs and appendages is impaired in mice lacking Sp6. Dev Dyn 237(4):883-92. [PubMed: 18297738] [MGI Ref ID J:132771]
Hirata T; Li P; Lanuza GM; Cocas LA; Huntsman MM; Corbin JG. 2009. Identification of distinct telencephalic progenitor pools for neuronal diversity in the amygdala. Nat Neurosci 12(2):141-9. [PubMed: 19136974] [MGI Ref ID J:146178]
Hoch RV; Soriano P. 2006. Context-specific requirements for Fgfr1 signaling through Frs2 and Frs3 during mouse development. Development 133(4):663-73. [PubMed: 16421190] [MGI Ref ID J:105920]
Hockemeyer D; Daniels JP; Takai H; de Lange T. 2006. Recent expansion of the telomeric complex in rodents: Two distinct POT1 proteins protect mouse telomeres. Cell 126(1):63-77. [PubMed: 16839877] [MGI Ref ID J:112185]
Holmes R; Chang Y; Soloway PD. 2006. Timing and sequence requirements defined for embryonic maintenance of imprinted DNA methylation at Rasgrf1. Mol Cell Biol 26(24):9564-70. [PubMed: 17030618] [MGI Ref ID J:117666]
Huang QQ; Hossain MM; Wu K; Parai K; Pope RM; Jin JP. 2008. Role of H2-calponin in regulating macrophage motility and phagocytosis. J Biol Chem 283(38):25887-99. [PubMed: 18617524] [MGI Ref ID J:141867]
Inagaki M; Komatsu Y; Scott G; Yamada G; Ray M; Ninomiya-Tsuji J; Mishina Y. 2008. Generation of a conditional mutant allele for Tab1 in mouse. Genesis 46(8):431-9. [PubMed: 18693278] [MGI Ref ID J:140317]
Iwawaki T; Akai R; Yamanaka S; Kohno K. 2009. Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability. Proc Natl Acad Sci U S A 106(39):16657-62. [PubMed: 19805353] [MGI Ref ID J:153222]
Jaworski A; Smith CL; Burden SJ. 2007. GA-Binding Protein Is Dispensable for Neuromuscular Synapse Formation and Synapse-Specific Gene Expression. Mol Cell Biol 27(13):5040-6. [PubMed: 17485447] [MGI Ref ID J:121823]
Jonassen JA; San Agustin J; Follit JA; Pazour GJ. 2008. Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease. J Cell Biol 183(3):377-84. [PubMed: 18981227] [MGI Ref ID J:141071]
Kallies A; Xin A; Belz GT; Nutt SL. 2009. Blimp-1 transcription factor is required for the differentiation of effector CD8(+) T cells and memory responses. Immunity 31(2):283-95. [PubMed: 19664942] [MGI Ref ID J:151858]
Kim IJ; Zhang Y; Yamagata M; Meister M; Sanes JR. 2008. Molecular identification of a retinal cell type that responds to upward motion. Nature 452(7186):478-82. [PubMed: 18368118] [MGI Ref ID J:134167]
Klein U; Casola S; Cattoretti G; Shen Q; Lia M; Mo T; Ludwig T; Rajewsky K; Dalla-Favera R. 2006. Transcription factor IRF4 controls plasma cell differentiation and class-switch recombination. Nat Immunol 7(7):773-82. [PubMed: 16767092] [MGI Ref ID J:112643]
Kruse SE; Watt WC; Marcinek DJ; Kapur RP; Schenkman KA; Palmiter RD. 2008. Mice with mitochondrial complex I deficiency develop a fatal encephalomyopathy. Cell Metab 7(4):312-20. [PubMed: 18396137] [MGI Ref ID J:134334]
Lan Y; Wang Q; Ovitt CE; Jiang R. 2007. A unique mouse strain expressing Cre recombinase for tissue-specific analysis of gene function in palate and kidney development. Genesis 45(10):618-24. [PubMed: 17941042] [MGI Ref ID J:128736]
Lee YK; Schmidt DR; Cummins CL; Choi M; Peng L; Zhang Y; Goodwin B; Hammer RE; Mangelsdorf DJ; Kliewer SA. 2008. Liver receptor homolog-1 regulates bile Acid homeostasis but is not essential for feedback regulation of bile Acid synthesis. Mol Endocrinol 22(6):1345-56. [PubMed: 18323469] [MGI Ref ID J:135822]
Levitt PS; Liu H; Manning C; Weiss RS. 2005. Conditional inactivation of the mouse Hus1 cell cycle checkpoint gene. Genomics 86(2):212-24. [PubMed: 15919177] [MGI Ref ID J:99339]
Li H; Cho SN; Evans CM; Dickey BF; Jeong JW; DeMayo FJ. 2008. Cre-mediated recombination in mouse Clara cells. Genesis 46(6):300-7. [PubMed: 18543320] [MGI Ref ID J:136676]
Lin Y; Liu G; Wang F. 2006. Generation of an Fgf9 conditional null allele. Genesis 44(3):150-4. [PubMed: 16496342] [MGI Ref ID J:106766]
Lin Y; Zhang J; Zhang Y; Wang F. 2007. Generation of an Frs2alpha conditional null allele. Genesis 45(9):554-9. [PubMed: 17868091] [MGI Ref ID J:128712]
Lu LF; Gavin MA; Rasmussen JP; Rudensky AY. 2007. G protein-coupled receptor 83 is dispensable for the development and function of regulatory T cells. Mol Cell Biol 27(23):8065-72. [PubMed: 17893329] [MGI Ref ID J:127791]
Ma XM; Kiraly DD; Gaier ED; Wang Y; Kim EJ; Levine ES; Eipper BA; Mains RE. 2008. Kalirin-7 is required for synaptic structure and function. J Neurosci 28(47):12368-82. [PubMed: 19020030] [MGI Ref ID J:142364]
Mao CA; Kiyama T; Pan P; Furuta Y; Hadjantonakis AK; Klein WH. 2008. Eomesodermin, a target gene of Pou4f2, is required for retinal ganglion cell and optic nerve development in the mouse. Development 135(2):271-80. [PubMed: 18077589] [MGI Ref ID J:130570]
Matkovich SJ; Diwan A; Klanke JL; Hammer DJ; Marreez Y; Odley AM; Brunskill EW; Koch WJ; Schwartz RJ; Dorn GW nd. 2006. Cardiac-specific ablation of G-protein receptor kinase 2 redefines its roles in heart development and beta-adrenergic signaling. Circ Res 99(9):996-1003. [PubMed: 17008600] [MGI Ref ID J:126501]
McCright B; Lozier J; Gridley T. 2006. Generation of new Notch2 mutant alleles. Genesis 44(1):29-33. [PubMed: 16397869] [MGI Ref ID J:105278]
Morikawa Y; D'Autreaux F; Gershon MD; Cserjesi P. 2007. Hand2 determines the noradrenergic phenotype in the mouse sympathetic nervous system. Dev Biol 307(1):114-26. [PubMed: 17531968] [MGI Ref ID J:122604]
Mu X; Fu X; Beremand PD; Thomas TL; Klein WH. 2008. Gene regulation logic in retinal ganglion cell development: Isl1 defines a critical branch distinct from but overlapping with Pou4f2. Proc Natl Acad Sci U S A 105(19):6942-7. [PubMed: 18460603] [MGI Ref ID J:146349]
Mukhopadhyay A; Jarrett J; Chlon T; Kessler JA. 2009. HeyL regulates the number of TrkC neurons in dorsal root ganglia. Dev Biol 334(1):142-51. [PubMed: 19631204] [MGI Ref ID J:153555]
Murchison ND; Price BA; Conner DA; Keene DR; Olson EN; Tabin CJ; Schweitzer R. 2007. Regulation of tendon differentiation by scleraxis distinguishes force-transmitting tendons from muscle-anchoring tendons. Development 134(14):2697-708. [PubMed: 17567668] [MGI Ref ID J:122742]
Murray SA; Carver EA; Gridley T. 2006. Generation of a Snail1 (Snai1) conditional null allele. Genesis 44(1):7-11. [PubMed: 16397867] [MGI Ref ID J:105294]
Nassar MA; Stirling LC; Forlani G; Baker MD; Matthews EA; Dickenson AH; Wood JN. 2004. Nociceptor-specific gene deletion reveals a major role for Nav1.7 (PN1) in acute and inflammatory pain. Proc Natl Acad Sci U S A 101(34):12706-11. [PubMed: 15314237] [MGI Ref ID J:92442]
Nukaya M; Moran S; Bradfield CA. 2009. The role of the dioxin-responsive element cluster between the Cyp1a1 and Cyp1a2 loci in aryl hydrocarbon receptor biology. Proc Natl Acad Sci U S A 106(12):4923-8. [PubMed: 19261855] [MGI Ref ID J:147150]
Olsen O; Funke L; Long JF; Fukata M; Kazuta T; Trinidad JC; Moore KA; Misawa H; Welling PA; Burlingame AL; Zhang M; Bredt DS. 2007. Renal defects associated with improper polarization of the CRB and DLG polarity complexes in MALS-3 knockout mice. J Cell Biol 179(1):151-64. [PubMed: 17923534] [MGI Ref ID J:134806]
Pan L; Deng M; Xie X; Gan L. 2008. ISL1 and BRN3B co-regulate the differentiation of murine retinal ganglion cells. Development 135(11):1981-90. [PubMed: 18434421] [MGI Ref ID J:134978]
Paul E; Cronan R; Weston PJ; Boekelheide K; Sedivy JM; Lee SY; Wiest DL; Resnick MB; Klysik JE. 2009. Disruption of Supv3L1 damages the skin and causes sarcopenia, loss of fat, and death. Mamm Genome 20(2):92-108. [PubMed: 19145458] [MGI Ref ID J:144991]
Prosser HM; Rzadzinska AK; Steel KP; Bradley A. 2008. Mosaic complementation demonstrates a regulatory role for myosin VIIa in actin dynamics of stereocilia. Mol Cell Biol 28(5):1702-12. [PubMed: 18160714] [MGI Ref ID J:132633]
Rawlins EL; Okubo T; Xue Y; Brass DM; Auten RL; Hasegawa H; Wang F; Hogan BL. 2009. The role of Scgb1a1+ Clara cells in the long-term maintenance and repair of lung airway, but not alveolar, epithelium. Cell Stem Cell 4(6):525-34. [PubMed: 19497281] [MGI Ref ID J:149822]
Rongvaux A; Galli M; Denanglaire S; Van Gool F; Dreze PL; Szpirer C; Bureau F; Andris F; Leo O. 2008. Nicotinamide phosphoribosyl transferase/pre-B cell colony-enhancing factor/visfatin is required for lymphocyte development and cellular resistance to genotoxic stress. J Immunol 181(7):4685-95. [PubMed: 18802071] [MGI Ref ID J:141448]
Rowan S; Cepko CL. 2004. Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter. Dev Biol 271(2):388-402. [PubMed: 15223342] [MGI Ref ID J:91498]
Schmahl J; Rizzolo K; Soriano P. 2008. The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev 22(23):3255-67. [PubMed: 19056881] [MGI Ref ID J:142042]
Skidmore JM; Cramer JD; Martin JF; Martin DM. 2008. Cre fate mapping reveals lineage specific defects in neuronal migration with loss of Pitx2 function in the developing mouse hypothalamus and subthalamic nucleus. Mol Cell Neurosci 37(4):696-707. [PubMed: 18206388] [MGI Ref ID J:135239]
Tabaries S; Lemieux M; Aubin J; Jeannotte L. 2007. Comparative analysis of Hoxa5 allelic series. Genesis 45(4):218-28. [PubMed: 17417799] [MGI Ref ID J:125040]
Tong Q; Ye C; McCrimmon RJ; Dhillon H; Choi B; Kramer MD; Yu J; Yang Z; Christiansen LM; Lee CE; Choi CS; Zigman JM; Shulman GI; Sherwin RS; Elmquist JK; Lowell BB. 2007. Synaptic glutamate release by ventromedial hypothalamic neurons is part of the neurocircuitry that prevents hypoglycemia. Cell Metab 5(5):383-93. [PubMed: 17488640] [MGI Ref ID J:129860]
Tong Q; Ye CP; Jones JE; Elmquist JK; Lowell BB. 2008. Synaptic release of GABA by AgRP neurons is required for normal regulation of energy balance. Nat Neurosci :. [PubMed: 18690230] [MGI Ref ID J:140858]
Wang P; Bowl MR; Bender S; Peng J; Farber L; Chen J; Ali A; Zhang Z; Alberts AS; Thakker RV; Shilatifard A; Williams BO; Teh BT. 2008. Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice. Mol Cell Biol 28(9):2930-40. [PubMed: 18212049] [MGI Ref ID J:135255]
Wang X; Li Y; Zhang X; Goodrich DW. 2007. An allelic series for studying the mouse Thoc1 gene. Genesis 45(1):32-7. [PubMed: 17211872] [MGI Ref ID J:117869]
Yamamoto M; Shook NA; Kanisicak O; Yamamoto S; Wosczyna MN; Camp JR; Goldhamer DJ. 2009. A multifunctional reporter mouse line for Cre- and FLP-dependent lineage analysis. Genesis 47(2):107-14. [PubMed: 19165827] [MGI Ref ID J:147304]
Yokoi F; Dang MT; Mitsui S; Li J; Li Y. 2008. Motor Deficits and Hyperactivity in Cerebral Cortex-specific Dyt1 Conditional Knockout Mice. J Biochem 143(1):39-47. [PubMed: 17956903] [MGI Ref ID J:130483]
Yokoi F; Dang MT; Mitsui S; Li Y. 2005. Exclusive paternal expression and novel alternatively spliced variants of epsilon-sarcoglycan mRNA in mouse brain. FEBS Lett 579(21):4822-8. [PubMed: 16099459] [MGI Ref ID J:101332]
Yoshioka J; Imahashi K; Gabel SA; Chutkow WA; Burds AA; Gannon J; Schulze PC; MacGillivray C; London RE; Murphy E; Lee RT. 2007. Targeted deletion of thioredoxin-interacting protein regulates cardiac dysfunction in response to pressure overload. Circ Res 101(12):1328-38. [PubMed: 17916779] [MGI Ref ID J:141487]
Zhang N; He YW. 2005. An essential role for c-FLIP in the efficient development of mature T lymphocytes. J Exp Med 202(3):395-404. [PubMed: 16043517] [MGI Ref ID J:100558]
Zhang N; He YW. 2005. The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol 174(11):6967-73. [PubMed: 15905539] [MGI Ref ID J:99012]
Zhang Y; Singh MK; Degenhardt KR; Lu MM; Bennett J; Yoshida Y; Epstein JA. 2009. Tie2Cre-mediated inactivation of plexinD1 results in congenital heart, vascular and skeletal defects. Dev Biol 325(1):82-93. [PubMed: 18992737] [MGI Ref ID J:143763]
Zhao J; Nassar MA; Gavazzi I; Wood JN. 2006. Tamoxifen-inducible NaV1.8-CreERT2 recombinase activity in nociceptive neurons of dorsal root ganglia. Genesis 44(8):364-71. [PubMed: 16850455] [MGI Ref ID J:114488]
Zhao T; Zhou X; Szabo N; Leitges M; Alvarez-Bolado G. 2007. Foxb1-driven Cre expression in somites and the neuroepithelium of diencephalon, brainstem, and spinal cord. Genesis 45(12):781-7. [PubMed: 18064677] [MGI Ref ID J:130491]
Zhu L; Gibson P; Currle DS; Tong Y; Richardson RJ; Bayazitov IT; Poppleton H; Zakharenko S; Ellison DW; Gilbertson RJ. 2009. Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature 457(7229):603-7. [PubMed: 19092805] [MGI Ref ID J:144215]
Zhu L; Peng JL; Harutyunyan KG; Garcia MD; Justice MJ; Belmont JW. 2007. Craniofacial, skeletal, and cardiac defects associated with altered embryonic murine Zic3 expression following targeted insertion of a PGK-NEO cassette. Front Biosci 12:1680-90. [PubMed: 17127413] [MGI Ref ID J:117748]
Zouvelou V; Passa O; Segklia K; Tsalavos S; Valenzuela DM; Economides AN; Graf D. 2009. Generation and functional characterization of mice with a conditional BMP7 allele. Int J Dev Biol 53(4):597-603. [PubMed: 19247966] [MGI Ref ID J:149134]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated and is mainatained on a 129S4/SvJaeSor background. Homozygotes are viable. Coat color expected from breeding:Agouti Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $112.90 Female or Male Homozygous for Gt(ROSA)26Sortm1(FLP1)Dym
Pairs /Price (US dollars $) Pair Genotype $225.80 Homozygous for Gt(ROSA)26Sortm1(FLP1)Dym x Homozygous for Gt(ROSA)26Sortm1(FLP1)Dym
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $146.80 Female or Male Homozygous for Gt(ROSA)26Sortm1(FLP1)Dym
Pairs /Price (US dollars $) Pair Genotype $293.60 Homozygous for Gt(ROSA)26Sortm1(FLP1)Dym x Homozygous for Gt(ROSA)26Sortm1(FLP1)Dym
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.