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Strain Name:

129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J

Stock Number:

003946

Availability:

Repository- Live


General Terms and Conditions

Genes & Alleles   Gt(ROSA)26Sor;   Gt(ROSA)26Sortm1(FLP1)Dym;


Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Susan Dymecki,   Harvard Medical School
Generation?+F16 (06-DEC-07)

Strain Description
This knock-in strain expresses a variant of the Saccharomyces cerevisiae FLP1 recombinase gene under the direction of the Gt(ROSA)26Sor promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wild type FLP at 37C and 40C, respectively. Homozygous mice for the knock-in allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Gt(ROSA)26Sor promoter drives expression in a constitutive fashion from preimplantation onward. Extensive target gene recombination can be achieved in most tissue types, including cells of the developing germ line. This mutant strain is suitable for use as a deleter strain and serves as a source of FLP1-expressing primary cell lines.

Strain Development
A knock-in construct (R26Fki) was built by inserting a FLPe cassette into a ROSA26 knock-in vector. This construct includes a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter. The construct was electroporated into 129S4/S4JaeSor-derived AK7 embryonic stem (ES) cells, thereby disrupting the Gt(ROSA)26Sor locus. Correctly targeted ES cells were utilized to generate chimeras.

Gene & Allele Details

Allele Symbol Gt(ROSA)26Sortm1(FLP1)Dym
Allele Name targeted mutation 1, Susan Dymecki
Common Name(s) CAGGS-Flpe; FLP; FLP deleter; FLPe; FLPeR; R26Fki; ROSA26::FLPe knock in; flipper;
Mutation Made By Susan Dymecki,   Harvard Medical School
Strain of Origin129S4/SvJaeSor
ES Cell Line NameAK7
ES Cell Line Strain129S4/SvJaeSor
Site of Expressionconstitutive expression of FLP1 recombinase from preimplantation onward and in most tissue types (including cells of the developing germ line)
Gene Symbol and Name Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Chromosome 6
Gene Common Name(s) AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26;
Molecular Note An enhanced flp cassette containing a splice acceptor site was ligated to a downstream PGK-neo cassette and inserted at the endogenous locus. Efficient frt-specific recombination in transgenic mice carrying a reporter gene was reported to reflect constituitive expression of the Gt(ROSA)26Sor from preimplantation onward. [MGI Ref ID J:66893] [MGI Ref ID J:92442]

Control Information

  Control
   002448 129S1/SvImJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Gt(ROSA)26Sortm1(FLP1)Dym

Colony Maintenance

Breeding & HusbandryThis strain originated and is mainatained on a 129S4/SvJaeSor background. Homozygotes are viable. Coat color expected from breeding:Agouti
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Gt(ROSA)26Sor
002292   129-Gt(ROSA)26Sor/J
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
003310   129S-Gt(ROSA)26Sortm1Sor/J
007689   129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
007708   B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
003474   B6.129S4-Gt(ROSA)26Sortm1Sor/J
002192   B6.129S7-Gt(ROSA)26Sor/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
003504   B6;129-Gt(ROSA)26Sortm1Sho/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
002073   B6;129S-Gt(ROSA)26Sor/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004598   B6;129S4-Gt(ROSA)26Sortm2Dym/J
007670   B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
002955   C.129S7-Gt(ROSA)26Sor/J
007900   C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J
005420   C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm1(HIF1A/luc)Kael/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
008159   STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
007577   STOCK Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
View Strains carrying other alleles of Gt(ROSA)26Sor     (37 strains)

Additional Web Information

Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
FLP-FRT System (FLP Recombinase Expression: Germline/Embryonic Expression)
Genetics Research (Mutagenesis and Transgenesis: FLP recombinase)

References

Selected Reference(s)

Farley FW; Soriano P; Steffen LS; Dymecki SM. 2000. Widespread recombinase expression using FLPeR (Flipper) mice Genesis 28(3-4):106-10. [PubMed: 11105051]  [MGI Ref ID J:66893]

Additional References

Price and Supply Information

Strain Name: 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J
Stock Number: 003946

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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