Strain Name:

B6.129P2-P2rx3tm1Ckn/J

Stock Number:

003951

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.P2-P2rx3tm1Ckn/J    (Changed: 23-OCT-13 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Debra A Cockayne,   Roche Bioscience

Description
Mice that are homozygous null for the P2rx3 gene are viable, fertile, normal in size and do not display any gross physical abnormalities. A significantly decreased nociceptive response (hindpaw lifting, licking, biting) is observed in response to injection of ATP, and to a lesser extent formalin. Responses to noxious thermal and mechanical stimuli are similar to that seen in wild-type mice. Aged mice may develop cutaneous hypersensitivity. Mice also exhibit urinary bladder hyporeflexia, characterized by decreased micturition (urination) frequencies and increased bladder capacities. Whole-cell patch-clamp analysis indicates that homozygote null mice lose the rapidly desensitizing ATP-induced currents in dorsal root ganglion. This strain is suitable for use in studies examining afferent sensory pathways.

Development
A targeting vector containing loxP flanked neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the P2rx3 gene encoding intron 1. The construct was injected into 129P2/OlaHsd-derived E14-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. A chimeric animal was obtained. Mutant mice were then backcrossed to C57BL/6J mice for five generations using a speed congenic protocol.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

P2rx3tm1Ckn/P2rx3tm1Ckn

        involves: 129P2/OlaHsd * C57BL/6
  • respiratory system phenotype
  • *normal* respiratory system phenotype
    • adult homozygotes (4-6 months of age) display normal resting ventilation during normoxia as well as normal ventilatory responses to varying levels of hypoxia (15%, 10%, and 7.5% O2) relative to wild-type controls   (MGI Ref ID J:86890)
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • adult homozygotes display normal carotid body function, as shown by a similar hypoxia-induced increase in sinus nerve activity in carotid body-sinus nerve preparations from homozygous mutant and wild-type mice   (MGI Ref ID J:86890)
    • no significant differences in the single-unit response to hypoxia are observed between mutant and wild-type carotid body-sinus nerve preparations (7.73 +/- 0.69 spikes/sec versus 8.22 +/- 0.74 spikes/sec, respectively)   (MGI Ref ID J:86890)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

P2rx3tm1Ckn related

Sensorineural Research
Nociception

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol P2rx3tm1Ckn
Allele Name targeted mutation 1, Debra A Cockayne
Allele Type Targeted (Null/Knockout)
Common Name(s) P2X3-;
Mutation Made By Debra Cockayne,   Roche Bioscience
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name P2rx3, purinergic receptor P2X, ligand-gated ion channel, 3
Chromosome 2
Gene Common Name(s) 4930513E20Rik; MGC:28163; P2X3; RIKEN cDNA 4930513E20 gene;
Molecular Note Exon 1 was replaced with a neomycin resistance gene via homologous recombination, thus deleting the translation initiation codon. Protein product was absent from dorsal root ganglion, spinal cord, and peripheral tissues of homozygous mutant animals as shown by immunohistochemistry. [MGI Ref ID J:65557]

Genotyping

Genotyping Information

Genotyping Protocols

P2rx3tm1Cknalternate1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cockayne DA; Hamilton SG; Zhu QM; Dunn PM; Zhong Y; Novakovic S; Malmberg AB; Cain G; Berson A; Kassotakis L; Hedley L; Lachnit WG; Burnstock G; McMahon SB; Ford AP. 2000. Urinary bladder hyporeflexia and reduced pain-related behaviour in P2X3-deficient mice Nature 407(6807):1011-5. [PubMed: 11069181]  [MGI Ref ID J:65557]

Additional References

P2rx3tm1Ckn related

Bian X; Ren J; DeVries M; Schnegelsberg B; Cockayne DA; Ford AP; Galligan JJ. 2003. Peristalsis is impaired in the small intestine of mice lacking the P2X3 subunit. J Physiol 551(Pt 1):309-22. [PubMed: 12813150]  [MGI Ref ID J:105400]

Cockayne DA; Dunn PM; Zhong Y; Rong W; Hamilton SG; Knight GE; Ruan HZ; Ma B; Yip P; Nunn P; McMahon SB; Burnstock G; Ford AP. 2005. P2X2 knockout mice and P2X2/P2X3 double knockout mice reveal a role for the P2X2 receptor subunit in mediating multiple sensory effects of ATP. J Physiol 567(Pt 2):621-39. [PubMed: 15961431]  [MGI Ref ID J:112498]

Finger TE; Danilova V; Barrows J; Bartel DL; Vigers AJ; Stone L; Hellekant G; Kinnamon SC. 2005. ATP signaling is crucial for communication from taste buds to gustatory nerves. Science 310(5753):1495-9. [PubMed: 16322458]  [MGI Ref ID J:103231]

Huang YA; Stone LM; Pereira E; Yang R; Kinnamon JC; Dvoryanchikov G; Chaudhari N; Finger TE; Kinnamon SC; Roper SD. 2011. Knocking out P2X receptors reduces transmitter secretion in taste buds. J Neurosci 31(38):13654-61. [PubMed: 21940456]  [MGI Ref ID J:177120]

Kiyatkin ME; Feng B; Schwartz ES; Gebhart GF. 2013. Combined genetic and pharmacological inhibition of TRPV1 and P2X3 attenuates colorectal hypersensitivity and afferent sensitization. Am J Physiol Gastrointest Liver Physiol 305(9):G638-48. [PubMed: 23989007]  [MGI Ref ID J:210567]

Rong W; Gourine AV; Cockayne DA; Xiang Z; Ford AP; Spyer KM; Burnstock G. 2003. Pivotal role of nucleotide P2X2 receptor subunit of the ATP-gated ion channel mediating ventilatory responses to hypoxia. J Neurosci 23(36):11315-21. [PubMed: 14672995]  [MGI Ref ID J:86890]

Shimizu I; Iida T; Guan Y; Zhao C; Raja SN; Jarvis MF; Cockayne DA; Caterina MJ. 2005. Enhanced thermal avoidance in mice lacking the ATP receptor P2X3. Pain 116(1-2):96-108. [PubMed: 15927378]  [MGI Ref ID J:103789]

Stratford JM; Finger TE. 2011. Central representation of postingestive chemosensory cues in mice that lack the ability to taste. J Neurosci 31(25):9101-10. [PubMed: 21697361]  [MGI Ref ID J:173522]

Vlaskovska M; Kasakov L; Rong W; Bodin P; Bardini M; Cockayne DA; Ford AP; Burnstock G. 2001. P2X3 knock-out mice reveal a major sensory role for urothelially released ATP. J Neurosci 21(15):5670-7. [PubMed: 11466438]  [MGI Ref ID J:111371]

Wang EC; Lee JM; Ruiz WG; Balestreire EM; von Bodungen M; Barrick S; Cockayne DA; Birder LA; Apodaca G. 2005. ATP and purinergic receptor-dependent membrane traffic in bladder umbrella cells. J Clin Invest 115(9):2412-22. [PubMed: 16110327]  [MGI Ref ID J:101204]

Wang Y; Mackes J; Chan S; Haughey NJ; Guo Z; Ouyang X; Furukawa K; Ingram DK; Mattson MP. 2006. Impaired long-term depression in P2X3 deficient mice is not associated with a spatial learning deficit. J Neurochem 99(5):1425-34. [PubMed: 17074061]  [MGI Ref ID J:119952]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together. The coat color expected from breeding is Black.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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