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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names 129S6-Tg(Prnp-GFP/Cre)1Blwd (Changed: 15-DEC-04 ) 98030B (Changed: 15-DEC-04 ) Tg(Prnp-GFP/Cre)98030Blw (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?+2 Donating Investigator Carrolee Barlow, Brain Cells Inc Description
Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice express the GFP/Cre fusion protein in widespread fashion. All tissues examined displayed Cre activity at an early (4-8 cell) embryonic stage. Germline expression is observed. Expression of GFP is less robust, being detectable in kidney tissue.Development
A transgenic construct containing a GFP/cre fusion gene driven by a mouse prion protein promoter was injected into fertilized 129S6/SvEv mouse eggs. A founder animal was obtained (98030B) and bred to wildtype 129S6/SvEv mice.
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
New 129 Nomenclature Bulletin
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Prnp-GFP/cre)1Blw | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Carrolee Barlow | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | 98030B; 98030B Prion.GFPCre; Cre(+); Prion-Cre; Tg(Prnp-GFP/cre)98030Blw; | ||
| Mutation Made By | Carrolee Barlow, Brain Cells Inc | ||
| Strain of Origin | 129S6/SvEvTac | ||
| Site of Expression | widespread expression (including germline) as early as the 4-8 cell embryonic stage; GFP expression is less robust | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Prnp, prion protein, mouse, laboratory | ||
| Driver Note | Prnp | ||
| General Note | This transgene is line 98030B of the laboratory in which the transgene originated. Homozygous transgenic mice are fertile and appear phenotypically normal. | ||
| Molecular Note | Expression of an in-frame fusion of cre to GFP was driven by a modified prion promoter. The resulting transgene expresses Cre recombinase in all tissues during embryogenesis including the germline. [MGI Ref ID J:90777] | ||
| Gene Symbol and Name | Tg(Prnp-GFP/cre)1Blw, transgene insertion 1, Carrolee Barlow | ||
| Chromosome | UN | ||
| Gene Common Name(s) | 98030B; 98030B Prion.GFPCre; Cre(+); Prion-Cre; Tg(Prnp-GFP/cre)98030Blw; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Scheel JR; Garrett LJ; Allen DM; Carter TA; Randolph-Moore L; Gambello MJ; Gage FH; Wynshaw-Boris A; Barlow C. 2003. An inbred 129SvEv GFPCre transgenic mouse that deletes loxP-flanked genes in all tissues. Nucleic Acids Res 31(10):e57. [PubMed: 12736323] [MGI Ref ID J:90777]
Additional References
Tg(Prnp-GFP/cre)1Blw relatedGuo X; Day TF; Jiang X; Garrett-Beal L; Topol L; Yang Y. 2004. Wnt/beta-catenin signaling is sufficient and necessary for synovial joint formation. Genes Dev 18(19):2404-17. [PubMed: 15371327] [MGI Ref ID J:93028]
Samardzija M; von Lintig J; Tanimoto N; Oberhauser V; Thiersch M; Reme CE; Seeliger M; Grimm C; Wenzel A. 2008. R91W mutation in Rpe65 leads to milder early-onset retinal dystrophy due to the generation of low levels of 11-cis-retinal. Hum Mol Genet 17(2):281-92. [PubMed: 17933883] [MGI Ref ID J:130076]
Stefanovic M; Markham NO; Parry EM; Garrett-Beal LJ; Cline AP; Gallagher PG; Low PS; Bodine DM. 2007. An 11-amino acid beta-hairpin loop in the cytoplasmic domain of band 3 is responsible for ankyrin binding in mouse erythrocytes. Proc Natl Acad Sci U S A 104(35):13972-7. [PubMed: 17715300] [MGI Ref ID J:145243]
Toyo-oka K; Hirotsune S; Gambello MJ; Zhou ZQ; Olson L; Rosenfeld MG; Eisenman R; Hurlin P; Wynshaw-Boris A. 2004. Loss of the Max-interacting protein Mnt in mice results in decreased viability, defective embryonic growth and craniofacial defects: relevance to Miller-Dieker syndrome. Hum Mol Genet 13(10):1057-67. [PubMed: 15028671] [MGI Ref ID J:90397]
de Guzman Strong C; Wertz PW; Wang C; Yang F; Meltzer PS; Andl T; Millar SE; Ho IC; Pai SY; Segre JA. 2006. Lipid defect underlies selective skin barrier impairment of an epidermal-specific deletion of Gata-3. J Cell Biol 175(4):661-70. [PubMed: 17116754] [MGI Ref ID J:119006]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry These mice originated and are maintained on a 129S6/SvEv background.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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