Strain Name: |
129S6-Tg(Prnp-GFP/cre)1Blw/J |
|---|---|
Stock Number: |
003960 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
129S6-Tg(Prnp-GFP/Cre)1Blwd (Changed: 15-DEC-04
) |
|
98030B (Changed: 15-DEC-04
) | |
|
Tg(Prnp-GFP/Cre)98030Blw (Changed: 15-DEC-04
) | |
| Genes & Alleles | GFP; Prnp; cre; Tg(Prnp-GFP/cre)1Blw; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Carrolee Barlow, Brain Cells Inc Generation F?+2 Strain Description
Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice express the GFP/Cre fusion protein in widespread fashion. All tissues examined displayed Cre activity at an early (4-8 cell) embryonic stage. Germline expression is observed. Expression of GFP is less robust, being detectable in kidney tissue.Strain Development
A transgenic construct containing a GFP/cre fusion gene driven by a mouse prion protein promoter was injected into fertilized 129S6/SvEv mouse eggs. A founder animal was obtained (98030B) and bred to wildtype 129S6/SvEv mice.
Gene & Allele Details
| Allele Symbol | Tg(Prnp-GFP/cre)1Blw | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Carrolee Barlow | ||
| Common Name(s) | 98030B; 98030B Prion.GFPCre; Cre(+); Prion-Cre; Tg(Prnp-GFP/cre)98030Blw; | ||
| Mutation Made By | Carrolee Barlow, Brain Cells Inc | ||
| Strain of Origin | 129S6/SvEvTac | ||
| Site of Expression | widespread expression (including germline) as early as the 4-8 cell embryonic stage; GFP expression is less robust | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Prnp, prion protein, mouse, laboratory | ||
| General Note | This transgene is line 98030B of the laboratory in which the transgene originated. Homozygous transgenic mice are fertile and appear phenotypically normal. | ||
| Molecular Note | Expression of an in-frame fusion of cre to GFP was driven by a modified prion promoter. The resulting transgene expresses Cre recombinase in all tissues during embryogenesis including the germline. [J:90777] | ||
Genotyping Protocols
Tg(balancer2)3Cgn(Cre), Tg(Sycp1-Cre)4Min, Tg(AlbCre)21Mgn, Tg(Ins2Cre)25Mgn, Tg(Mx1-Cre)1Cgn
Colony Maintenance
| Breeding & Husbandry | These mice originated and are maintained on a 129S6/SvEv background. |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX Notes, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
New 129 Nomenclature Bulletin
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox-System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesScheel JR; Garrett LJ; Allen DM; Carter TA; Randolph-Moore L; Gambello MJ; Gage FH; Wynshaw-Boris A; Barlow C. 2003. An inbred 129SvEv GFPCre transgenic mouse that deletes loxP-flanked genes in all tissues. Nucleic Acids Res 31(10):e57. [PubMed: 12736323] [J:90777]
Price and Supply Information
| Strain Name: | 129S6-Tg(Prnp-GFP/cre)1Blw/J |
| Stock Number: | 003960 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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