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Former Names B6.Cg-Tg(Syn1-Cre)671Jxm (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Jamey Marth, University of California at San Diego Description
These transgenic mice express Cre recombinase under the direction of a synapsin promoter. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is detected in neuronal cells by embryonic day 12.5.Development
A transgenic construct containing a Cre recombinase gene under the direction of a synapsin promoter was injected into fertilized B6;CBAF1 mouse eggs. Founder animals were bred to wildtype C57BL/6NHsd mice.
| Control | ||
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| Noncarrier | ||
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Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
Introduction to Cre-lox technology
JAX® NOTES, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.
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Research Applications
This mouse can be used to support research in many areas including:
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Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
| Allele Symbol | Tg(Syn1-cre)671Jxm | ||
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| Allele Name | transgene insertion 671, Jamey Marth | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Syn-cre; SynI-Cre; | ||
| Mutation Made By | Jamey Marth, University of California at San Diego | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | neuronal cells by embryonic day 12.5 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Syn1, synapsin I, rat | ||
| Gene Symbol and Name | Tg(Syn1-cre)671Jxm, transgene insertion 671, Jamey Marth | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Syn-cre; SynI-Cre; | ||
| Driver Note | Syn1 | ||
| General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a rat synapsin I promoter. Cre recombinase activity is detected in neuronal cells, including brain, spinal cord and DRGs, as early as E12.5, as well as in neurons in adult. [MGI Ref ID J:68558] | ||
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
Zhu Y; Romero MI; Ghosh P; Ye Z; Charnay P; Rushing EJ; Marth JD; Parada LF. 2001. Ablation of NF1 function in neurons induces abnormal development of cerebral cortex and reactive gliosis in the brain. Genes Dev 15(7):859-76. [PubMed: 11297510] [MGI Ref ID J:68558]
Mori H; Hanada R; Hanada T; Aki D; Mashima R; Nishinakamura H; Torisu T; Chien KR; Yasukawa H; Yoshimura A. 2004. Socs3 deficiency in the brain elevates leptin sensitivity and confers resistance to diet-induced obesity. Nat Med 10(7):739-43. [PubMed: 15208705] [MGI Ref ID J:91796]
Tg(Syn1-cre)671Jxm relatedCai J; Chen Y; Cai WH; Hurlock EC; Wu H; Kernie SG; Parada LF; Lu QR. 2007. A crucial role for Olig2 in white matter astrocyte development. Development 134(10):1887-99. [PubMed: 17428828] [MGI Ref ID J:121422]
Cohen P; Zhao C; Cai X; Montez JM; Rohani SC; Feinstein P; Mombaerts P; Friedman JM. 2001. Selective deletion of leptin receptor in neurons leads to obesity. J Clin Invest 108(8):1113-21. [PubMed: 11602618] [MGI Ref ID J:72241]
Corbetta S; Gualdoni S; Ciceri G; Monari M; Zuccaro E; Tybulewicz VL; de Curtis I. 2009. Essential role of Rac1 and Rac3 GTPases in neuronal development. FASEB J 23(5):1347-57. [PubMed: 19126596] [MGI Ref ID J:148225]
Cui Y; Costa RM; Murphy GG; Elgersma Y; Zhu Y; Gutmann DH; Parada LF; Mody I; Silva AJ. 2008. Neurofibromin regulation of ERK signaling modulates GABA release and learning. Cell 135(3):549-60. [PubMed: 18984165] [MGI Ref ID J:147614]
DeFalco J; Tomishima M; Liu H; Zhao C; Cai X; Marth JD; Enquist L; Friedman JM. 2001. Virus-assisted mapping of neural inputs to a feeding center in the hypothalamus. Science 291(5513):2608-13. [PubMed: 11283374] [MGI Ref ID J:68414]
Di Nardo A; Kramvis I; Cho N; Sadowski A; Meikle L; Kwiatkowski DJ; Sahin M. 2009. Tuberous sclerosis complex activity is required to control neuronal stress responses in an mTOR-dependent manner. J Neurosci 29(18):5926-37. [PubMed: 19420259] [MGI Ref ID J:148479]
Ferguson C; Hardy SL; Werner DF; Hileman SM; Delorey TM; Homanics GE. 2007. New insight into the role of the beta3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout. BMC Neurosci 8:85. [PubMed: 17927825] [MGI Ref ID J:127877]
Hasue F; Kuwaki T; Kisanuki YY; Yanagisawa M; Moriya H; Fukuda Y; Shimoyama M. 2005. Increased sensitivity to acute and persistent pain in neuron-specific endothelin-1 knockout mice. Neuroscience 130(2):349-58. [PubMed: 15664691] [MGI Ref ID J:95930]
He XP; Kotloski R; Nef S; Luikart BW; Parada LF; McNamara JO. 2004. Conditional deletion of TrkB but not BDNF prevents epileptogenesis in the kindling model. Neuron 43(1):31-42. [PubMed: 15233915] [MGI Ref ID J:91467]
Hinoi E; Gao N; Jung DY; Yadav V; Yoshizawa T; Myers MG Jr; Chua SC Jr; Kim JK; Kaestner KH; Karsenty G. 2008. The sympathetic tone mediates leptin's inhibition of insulin secretion by modulating osteocalcin bioactivity. J Cell Biol 183(7):1235-42. [PubMed: 19103808] [MGI Ref ID J:145998]
Huang YZ; Pan E; Xiong ZQ; McNamara JO. 2008. Zinc-mediated transactivation of TrkB potentiates the hippocampal mossy fiber-CA3 pyramid synapse. Neuron 57(4):546-58. [PubMed: 18304484] [MGI Ref ID J:135687]
Kleinridders A; Pogoda HM; Irlenbusch S; Smyth N; Koncz C; Hammerschmidt M; Bruning JC. 2009. PLRG1 is an essential regulator of cell proliferation and apoptosis during vertebrate development and tissue homeostasis. Mol Cell Biol 29(11):3173-85. [PubMed: 19307306] [MGI Ref ID J:149153]
Li Y; Lim S; Hoffman D; Aspenstrom P; Federoff HJ; Rempe DA. 2009. HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport. J Cell Biol 185(6):1065-81. [PubMed: 19528298] [MGI Ref ID J:150420]
Li Y; Luikart BW; Birnbaum S; Chen J; Kwon CH; Kernie SG; Bassel-Duby R; Parada LF. 2008. TrkB regulates hippocampal neurogenesis and governs sensitivity to antidepressive treatment. Neuron 59(3):399-412. [PubMed: 18701066] [MGI Ref ID J:147252]
Ma L; Reis G; Parada LF; Schuman EM. 1999. Neuronal NT-3 is not required for synaptic transmission or long-term potentiation in area CA1 of the adult rat hippocampus. Learn Mem 6(3):267-75. [PubMed: 10492008] [MGI Ref ID J:82354]
Mastronardi C; Smiley GG; Raber J; Kusakabe T; Kawaguchi A; Matagne V; Dietzel A; Heger S; Mungenast AE; Cabrera R; Kimura S; Ojeda SR. 2006. Deletion of the Ttf1 gene in differentiated neurons disrupts female reproduction without impairing basal ganglia function. J Neurosci 26(51):13167-79. [PubMed: 17182767] [MGI Ref ID J:116686]
May P; Rohlmann A; Bock HH; Zurhove K; Marth JD; Schomburg ED; Noebels JL; Beffert U; Sweatt JD; Weeber EJ; Herz J. 2004. Neuronal LRP1 Functionally Associates with Postsynaptic Proteins and Is Required for Normal Motor Function in Mice. Mol Cell Biol 24(20):8872-83. [PubMed: 15456862] [MGI Ref ID J:93329]
Meikle L; Pollizzi K; Egnor A; Kramvis I; Lane H; Sahin M; Kwiatkowski DJ. 2008. Response of a neuronal model of tuberous sclerosis to mammalian target of rapamycin (mTOR) inhibitors: effects on mTORC1 and Akt signaling lead to improved survival and function. J Neurosci 28(21):5422-32. [PubMed: 18495876] [MGI Ref ID J:136366]
Meikle L; Talos DM; Onda H; Pollizzi K; Rotenberg A; Sahin M; Jensen FE; Kwiatkowski DJ. 2007. A mouse model of tuberous sclerosis: neuronal loss of Tsc1 causes dysplastic and ectopic neurons, reduced myelination, seizure activity, and limited survival. J Neurosci 27(21):5546-58. [PubMed: 17522300] [MGI Ref ID J:121858]
Mori H; Hanada R; Hanada T; Aki D; Mashima R; Nishinakamura H; Torisu T; Chien KR; Yasukawa H; Yoshimura A. 2004. Socs3 deficiency in the brain elevates leptin sensitivity and confers resistance to diet-induced obesity. Nat Med 10(7):739-43. [PubMed: 15208705] [MGI Ref ID J:91796]
O'Donnell N; Zachara NE; Hart GW; Marth JD. 2004. Ogt-dependent X-chromosome-linked protein glycosylation is a requisite modification in somatic cell function and embryo viability. Mol Cell Biol 24(4):1680-90. [PubMed: 14749383] [MGI Ref ID J:93112]
Ramanan N; Shen Y; Sarsfield S; Lemberger T; Schutz G; Linden DJ; Ginty DD. 2005. SRF mediates activity-induced gene expression and synaptic plasticity but not neuronal viability. Nat Neurosci 8(6):759-67. [PubMed: 15880109] [MGI Ref ID J:99232]
Rempe D; Vangeison G; Hamilton J; Li Y; Jepson M; Federoff HJ. 2006. Synapsin I Cre transgene expression in male mice produces germline recombination in progeny. Genesis 44(1):44-9. [PubMed: 16419044] [MGI Ref ID J:105271]
Romero MI; Lin L; Lush ME; Lei L; Parada LF; Zhu Y. 2007. Deletion of Nf1 in neurons induces increased axon collateral branching after dorsal root injury. J Neurosci 27(8):2124-34. [PubMed: 17314307] [MGI Ref ID J:118374]
Ruzankina Y; Pinzon-Guzman C; Asare A; Ong T; Pontano L; Cotsarelis G; Zediak VP; Velez M; Bhandoola A; Brown EJ. 2007. Deletion of the developmentally essential gene ATR in adult mice leads to age-related phenotypes and stem cell loss. Cell Stem Cell 1(1):113-26. [PubMed: 18371340] [MGI Ref ID J:123200]
Sahay A; Molliver ME; Ginty DD; Kolodkin AL. 2003. Semaphorin 3F is critical for development of limbic system circuitry and is required in neurons for selective CNS axon guidance events. J Neurosci 23(17):6671-80. [PubMed: 12890759] [MGI Ref ID J:84687]
Shi Y; Yadav VK; Suda N; Liu XS; Guo XE; Myers MG Jr; Karsenty G. 2008. Dissociation of the neuronal regulation of bone mass and energy metabolism by leptin in vivo. Proc Natl Acad Sci U S A 105(51):20529-33. [PubMed: 19074282] [MGI Ref ID J:142673]
Smith KM; Ohkubo Y; Maragnoli ME; Rasin MR; Schwartz ML; Sestan N; Vaccarino FM. 2006. Midline radial glia translocation and corpus callosum formation require FGF signaling. Nat Neurosci 9(6):787-97. [PubMed: 16715082] [MGI Ref ID J:110263]
Tamai K; Toyoshima M; Tanaka N; Yamamoto N; Owada Y; Kiyonari H; Murata K; Ueno Y; Ono M; Shimosegawa T; Yaegashi N; Watanabe M; Sugamura K. 2008. Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration. Am J Pathol 173(6):1806-17. [PubMed: 19008375] [MGI Ref ID J:141391]
Xia CH; Roberts EA; Her LS; Liu X; Williams DS; Cleveland DW; Goldstein LS. 2003. Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A. J Cell Biol 161(1):55-66. [PubMed: 12682084] [MGI Ref ID J:82911]
Yang X; Klein R; Tian X; Cheng HT; Kopan R; Shen J. 2004. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev Biol 269(1):81-94. [PubMed: 15081359] [MGI Ref ID J:90392]
Ye Z; Marth JD. 2004. N-glycan branching requirement in neuronal and postnatal viability. Glycobiology 14(6):547-58. [PubMed: 15044398] [MGI Ref ID J:94770]
Zhu Y; Harada T; Liu L; Lush ME; Guignard F; Harada C; Burns DK; Bajenaru ML; Gutmann DH; Parada LF. 2005. Inactivation of NF1 in CNS causes increased glial progenitor proliferation and optic glioma formation. Development 132(24):5577-88. [PubMed: 16314489] [MGI Ref ID J:104342]
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;CBAF1 background and has been backcrossed to wildtype C57BL/6NHsd mice for at least 5 generations. Coat color expected from breeding:Black
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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