Strain Name: |
C3Bir.129P2(B6)-Il10tm1Cgn/LtJ |
|---|---|
Stock Number: |
003968 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
C3Bir.129P2(B6)-Il10tm1Cgn/J (Changed: 14-DEC-05
) |
| Genes & Alleles | Il10; Il10tm1Cgn; Pde6b; Pde6brd1; Tlr4; Tlr4Lps-d; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Edward Leiter, The Jackson Laboratory Generation N16p (30-MAY-04) Strain Description
Mice homozygous for the Il10tm1Cgn targeted mutation are viable and fertile when housed under specific pathogen free (SPF) conditions. Under conventional housing conditions, Il10-deficiency is associated with altered lymphocyte and myeloid profiles, elevated serum amyloid A levels, altered responses to inflammatory or autoimmune stimuli (both endogenous and induced), increased prevalence of colorectal adenocarcinoma (especially on 129/Sv and, to a lesser extent, BALB/c genetic background), and spontaneous development of chronic enterocolitis (see below). As The Jackson Laboratory Repository maintains these mice at high health status conditions (high SPF), the observed or experimentally-induced Il10-deficient phenotype may vary from that previously published using mice from conventional mouse rooms. These IL-10 mutant mice may be useful studying inflammatory bowel disease (IBD) (Crohn's disease (CD) and/or colitis), cancer, innate and adaptive immunity, and many other areas of inflammatory or autoimmunity research.The onset and severity of both spontaneous and experimentally-induced inflammatory phenotype of Il10-deficient mice is strongly influenced by the genetic background and the husbandry conditions (specific health status/commensal flora) of the vivaria in which mice are maintained.
For example, inflammatory bowel disease (IBD; colitis and Crohn's disease) severity in mouse models is dependent upon interactions between specific genetic background and environmental factors (an as yet undefined component of the enteric flora of which Helicobacter spp. appear to be associated, but not specifically the environmental trigger). Both spontaneous and induced models of IBD demonstrate that susceptibility to intestinal inflammation varies markedly among inbred strains of mice. Generally, for Il10-deficient models on defined genetic backgrounds, the severity of colitis-related characteristics is most severe on C3H/HeJBir (Stock No. 004326 and Stock No. 003968) or 129/Sv (Stock No. 004368), intermediate on BALB/cJ (Stock No. 004333) or NOD/Lt (Stock No. 004266), and least severe on C57BL/10 (Stock No. 002250) or C57BL/6J (Stock No. 002251). Furthermore, the husbandry conditions (specific health status/commensal flora) of the vivaria in which mice are maintained significantly alter the onset and severity of spontaneous IBD; higher SPF conditions are associated with attenuated colitis. Il10-deficient mice on both the C3H/HeJBir and C57BL/6J genetic backgrounds exhibit a significant increase in peripheral blood granulocyte populations upon lesion development and this metric may be used as a robust non-lethal assessment of Il10-deficiency induced colitis onset and severity. Other indications of Il10-deficiency induced colitic lesion onset may include perianal ulceration (C3H/HeJBir background) or rectal prolapse (C57BL/6J background).
For a more detailed description please refer to the JAX Notes Fall 1997 article.
Strain Development
The Il10tm1Cgn mutation was created by in the Laboratory of Dr. Werner Muller at the University of Cologne. Briefly, a targeting vector was designed to replace codons 5-55 of exon 1 of the targeted gene with a 24 bp linker (providing a termination codon) and a neo expression cassette, as well as introduce a termination codon into exon 3. The construct was electroporated into 129P2/OlaHsd-derived E14-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females to establish the mutant colony on a mixed B6;129P2 genetic background. Subsequently, mutant mice were backcrossed to the C3H/HeJBir genetic background for at least 12 generations to generate this strain.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Il10tm1Cgn | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, University of Cologne | ||
| Common Name(s) | IL-10 KO; IL-10-; IL-10KO; IL-10KO; Il10-; | ||
| Mutation Made By | Ralf Kuhn, University of Cologne | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14.1 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Il10, interleukin 10 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | CSIF; IL-10; IL10A; Il-10; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; | ||
| Molecular Note | A 500 bp genomic fragment containing codons 5-55 was replaced with a linker containing a termination codon followed by a neomycin cassette. A termination codon was also introduced into exon 3. No IL10 activity was detectable in ELISA assays in supernatants of in vitro splenic T cells derived from homozygous mice. | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Common Name(s) | rd; rd-1; rd1; rodless retina; | ||
| Allele Symbol | Tlr4Lps-d | ||
| Allele Name | defective lipopolysaccharide response | ||
| Common Name(s) | TLR4-Mu; Tlr4-; Tlr4d; TlrLps-d; lpsd; | ||
| Strain of Origin | C3H/HeJ | ||
| Gene Symbol and Name | Tlr4, toll-like receptor 4 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | ARMD10; CD284; Lps; RAS-like, family 2, locus 8; Rasl2-8; TOLL; hToll; lipopolysaccharide response; | ||
| General Note |
C3H/HeJ mice carry this allele. Various combinations of Lps-associated traits have been followed in crosses between C3H/HeJ and other C3H substrains, and the traits have in all cases segregated together (J:30692, J:5557, J:5593, J:5938). Some of the traits show dominance of the Tlr4lps-n allele; others, including Tlr4Lps-d, show codominance. Genbank ID for this allele: AF095353 | ||
| Molecular Note | This allele corresponds to a mutation in the third exon of the gene. A C to A substitution at nucleotide position 2342 results in an amino acid substitution that replaces proline with histidine at position 712. [MGI Ref ID J:51522] [MGI Ref ID J:53519] [MGI Ref ID J:57938] | ||
| Allele | Control | |
|---|---|---|
| Il10tm1Cgn | None Available | |
| Considerations for Choosing Controls | ||
Il10tm1Cgn
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred together. As homozygous mice are more susceptible to pathogenic bacteria, high specific pathogen-free (SPF) conditions are recommended for optimal breeding. However, the onset and severity of both the spontaneous and experimentally-induced inflammatory phenotype of Il10-deficient mice is strongly influenced by the genetic background and the husbandry conditions (specific health status/commensal flora) of the vivaria in which mice are maintained and such high SPF conditions may attenuate the desired Il10-deficient phenotype. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying Il10tm1Cgn allele
004368 129(B6)-Il10tm1Cgn/J 002250 B10.129P2(B6)-Il10tm1Cgn/J 002251 B6.129P2-Il10tm1Cgn/J 005912 B6.Cg-Il10tm1Cgn (D3Mit49-D3Mit348)/Lt 004333 C.129P2(B6)-Il10tm1Cgn/J 004326 C3Bir.129P2(B6)-Il10tm1Cgn/Lt 005346 NOD.Cg-Il10tm1Cgn Casp1tm1Sesh/LtJ 004228 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/Dvs 004291 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ 004266 NOD.Cg-Il10tm1Cgn/DvsJ View Strains carrying Il10tm1Cgn (10 strains)
Strains carrying Pde6brd1 allele
View Strains carrying Pde6brd1 (74 strains)
Strains carrying Tlr4Lps-d allele
002930 C.C3-Tlr4Lps-d/J 005973 C3Bir.129P2(B6)-Il10C3Bir/LtJ 004326 C3Bir.129P2(B6)-Il10tm1Cgn/Lt 000659 C3H/HeJ 005972 C3H/HeJBirLtJ View Strains carrying Tlr4Lps-d (5 strains)
Strains carrying other alleles of Pde6b
004297 B6.CXB1-Pde6brd10/J 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J View Strains carrying other alleles of Pde6b (8 strains)
Strains carrying other alleles of Tlr4
007227 B6.B10ScN-Tlr4lps-del/JthJ 003752 C57BL/10ScNJ View Strains carrying other alleles of Tlr4 (2 strains)
Congenic Nomenclature
Il10tm1Cgn related
Pde6brd1 relatedCancer Research
Growth Factors/Receptors/Cytokines
Hematological Research
Anemia, Iron Deficiency and Transport Defects (hemolytic)
Immunological Defects
Immunology and Inflammation Research
Autoimmunity
Growth Factors/Receptors/Cytokines
Immunodeficiency
Inflammation (Inflammatory bowel disease)
Tlr4Lps-d relatedMouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (Tlr deficiency)
Immunodeficiency (Tlr deficiency)
Inflammation (Tlr deficiency)
Selected Reference(s)
Additional ReferencesBristol IJ; Farmer MA; Cong Y; Zheng XX; Strom TB; Elson CO; Sundberg JP; Leiter EH. 2000. Heritable susceptibility for colitis in mice induced by IL-10 deficiency. Inflamm Bowel Dis 6(4):290-302. [PubMed: 11149562] [MGI Ref ID J:109882]
| Strain Name: | C3Bir.129P2(B6)-Il10tm1Cgn/LtJ |
| Stock Number: | 003968 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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