Strain Name:

B6.129S4-Itgamtm1Myd/J

Stock Number:

003991

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Availability:

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These mice lack integrin alpha M expression and exhibit diminished neutrophil activation during inflammation.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
GenerationN10+N2F9 (24-JAN-14)
Generation Definitions
 
Donating Investigator Tanya Mayadas,   Brigham and Women's Hosp/Harvard Med Sch

Description
Mice that are homozygous for the Itgamtm1Myd targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No Itgam protein is detected in homozygous mutant neutrophils. Homozygous null animals have a diminished ability to clear thioglycollate-induced neutrophils, have reduced mast cell numbers in the dorsal skin and peritoneal wall/cavity, and are less susceptible to cerebral ischemia/reperfusion injury. Neutrophils from these animals are deficient at spreading, phagocytosing complement-opsonized particles, and in several Fc-mediated functions. They also exhibit impaired oxidative burst and a diminished responsiveness in LPS- and taxol-mediated gene expression.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Itgam gene encoding the translational initiation codon and 15 amino acids of the signal peptide. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into 3.5 day C57BL/6 blastocysts and chimeric animals obtained.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Systemic Lupus Erythematosus, Susceptibility to, 6; SLEB6   (ITGAM)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Itgamtm1Myd/Itgamtm1Myd

        B6.129S4-Itgamtm1Myd
  • immune system phenotype
  • abnormal inflammatory response
    • following exposure to LPS then challenge with TNF at the same site to initiate a local Shwartman response, mice fail to exhibit hemorrhage and have fewer thrombus-occluded vessels and reduced fibrin compared to the thrombohemorrhagic vasculitis observed in wild-type mice   (MGI Ref ID J:113463)
    • however, neutrophil accumulation at the site of the Shwartman response is normal and adoptive transfer of neutrophils into a wild-type mouse elicits a normal local Shwartman response   (MGI Ref ID J:113463)
  • abnormal neutrophil morphology
    • neutrophils that accumulate at the site of a local Shwartman response are round instead of flattened as in wild-type mice   (MGI Ref ID J:113463)
  • abnormal neutrophil physiology
    • neutrophil degranulation after adhesion is decreased compared to in wild-type mice   (MGI Ref ID J:113463)
  • homeostasis/metabolism phenotype
  • abnormal blood homeostasis
    • mouse serum fails to support neutrophil adhesion unlike serum from wild-type mice   (MGI Ref ID J:113463)
  • hematopoietic system phenotype
  • abnormal neutrophil morphology
    • neutrophils that accumulate at the site of a local Shwartman response are round instead of flattened as in wild-type mice   (MGI Ref ID J:113463)
  • abnormal neutrophil physiology
    • neutrophil degranulation after adhesion is decreased compared to in wild-type mice   (MGI Ref ID J:113463)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Itgamtm1Myd/Itgamtm1Myd

        involves: 129S4/SvJae * BALB/c * C57BL/6
  • cellular phenotype
  • abnormal leukocyte adhesion
    • neutrophils from null animals are significantly impaired with respect to their ability to adhere to the endothelium in response to leukotriene B4   (MGI Ref ID J:38052)
  • decreased apoptosis
    • in cultured total leukocyte populations from harvested peritoneal exudates, extravasated null neutrophils show a 56% and 45% reduction in apoptosis after 6 and 8 hours in culture respectively, compared to wild-type neutrophils   (MGI Ref ID J:38052)
  • hematopoietic system phenotype
  • abnormal leukocyte adhesion
    • neutrophils from null animals are significantly impaired with respect to their ability to adhere to the endothelium in response to leukotriene B4   (MGI Ref ID J:38052)
  • impaired neutrophil phagocytosis
    • null neurophils isolated from peritoneal lavage of thioglycollate-treated mice are unable to phagocytose complement-opsonized particles; neutrophil spreading on glass is attenuated in null neutrophils; these neutrophils also show a 60% reduction in the oxidative burst associated with phagocytosis   (MGI Ref ID J:38052)
  • increased eosinophil cell number
    • mutants show increased eosinophil accumulation at 10.5 hours, 48 hours and 5 days in the model of peritonitis   (MGI Ref ID J:38052)
  • increased neutrophil cell number
    • null animals have 2-fold more neutrophils in the peritoneal exudates after 2 hours in an experimental model of peritonitis than did wild-type animals   (MGI Ref ID J:38052)
  • immune system phenotype
  • abnormal leukocyte adhesion
    • neutrophils from null animals are significantly impaired with respect to their ability to adhere to the endothelium in response to leukotriene B4   (MGI Ref ID J:38052)
  • impaired neutrophil phagocytosis
    • null neurophils isolated from peritoneal lavage of thioglycollate-treated mice are unable to phagocytose complement-opsonized particles; neutrophil spreading on glass is attenuated in null neutrophils; these neutrophils also show a 60% reduction in the oxidative burst associated with phagocytosis   (MGI Ref ID J:38052)
  • increased eosinophil cell number
    • mutants show increased eosinophil accumulation at 10.5 hours, 48 hours and 5 days in the model of peritonitis   (MGI Ref ID J:38052)
  • increased neutrophil cell number
    • null animals have 2-fold more neutrophils in the peritoneal exudates after 2 hours in an experimental model of peritonitis than did wild-type animals   (MGI Ref ID J:38052)

Itgamtm1Myd/Itgamtm1Myd

        involves: 129S4/SvJae * C57BL/6
  • nervous system phenotype
  • decreased cerebral infarction size
    • after 3 hours of focal ischemia and 21 hours of reperfusion, infarct volume in wild-type mice is 79.4 cubic mm versus 58.9 cubic mm in homozygous animals (26% reduction in tissue injury)   (MGI Ref ID J:103873)
  • hematopoietic system phenotype
  • decreased neutrophil cell number
    • reduction in infarct size is accompanied by a 50% reduction in neutrophils in the periphery of the infarct area, although this is not statistically significant   (MGI Ref ID J:103873)
  • immune system phenotype
  • decreased neutrophil cell number
    • reduction in infarct size is accompanied by a 50% reduction in neutrophils in the periphery of the infarct area, although this is not statistically significant   (MGI Ref ID J:103873)
  • homeostasis/metabolism phenotype
  • decreased cerebral infarction size
    • after 3 hours of focal ischemia and 21 hours of reperfusion, infarct volume in wild-type mice is 79.4 cubic mm versus 58.9 cubic mm in homozygous animals (26% reduction in tissue injury)   (MGI Ref ID J:103873)

Itgamtm1Myd/Itgamtm1Myd

        involves: 129S4/SvJae
  • immune system phenotype
  • abnormal leukocyte tethering or rolling
    • absence of rolling following needle puncture of mesenteric veins   (MGI Ref ID J:198197)
  • impaired neutrophil phagocytosis   (MGI Ref ID J:155479)
  • cellular phenotype
  • abnormal leukocyte tethering or rolling
    • absence of rolling following needle puncture of mesenteric veins   (MGI Ref ID J:198197)
  • hematopoietic system phenotype
  • abnormal leukocyte tethering or rolling
    • absence of rolling following needle puncture of mesenteric veins   (MGI Ref ID J:198197)
  • impaired neutrophil phagocytosis   (MGI Ref ID J:155479)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Itgamtm1Myd related

Cell Biology Research
Defects in Cell Adhesion Molecules

Diabetes and Obesity Research
Obesity Without Diabetes
      adult onset

Immunology, Inflammation and Autoimmunity Research
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Itgamtm1Myd
Allele Name targeted mutation 1, Tanya N Mayadas
Allele Type Targeted (Null/Knockout)
Common Name(s) CD11b KO; CD11b/CD18 deficiency; CR3-; Cd11b-; Itgamtm1Tmn; Mac-1-;
Mutation Made By Tanya Mayadas,   Brigham and Women's Hosp/Harvard Med Sch
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Itgam, integrin alpha M
Chromosome 7
Gene Common Name(s) CD11B; CD11B (p170); CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; MAC-1; MAC1A; MO1A; Mac-1 alpha; Mac-1a; RIKEN cDNA F730045J24 gene; SLEB6; complement component receptor 3 alpha; complement receptor type 3; lymphocyte antigen 40; macrophage antigen alpha chain (integrin);
Molecular Note Replacement of the exon encoding the translational initiation codon and 15 amino acids of the signal peptide with a neomycin cassette. [MGI Ref ID J:38052]

Genotyping

Genotyping Information

Genotyping Protocols

Itgamtm1Myd, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Coxon A; Rieu P; Barkalow FJ; Askari S; Sharpe AH; von Andrian UH; Arnaout MA; Mayadas TN. 1996. A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation. Immunity 5(6):653-66. [PubMed: 8986723]  [MGI Ref ID J:38052]

Additional References

Dong ZM; Gutierrez-Ramos JC; Coxon A; Mayadas TN; Wagner DD. 1997. A new class of obesity genes encodes leukocyte adhesion receptors. Proc Natl Acad Sci U S A 94(14):7526-30. [PubMed: 9207125]  [MGI Ref ID J:41751]

Hu C; Mayadas-Norton T; Tanaka K; Chan J; Salgame P. 2000. Mycobacterium tuberculosis infection in complement receptor 3-deficient mice. J Immunol 165(5):2596-602. [PubMed: 10946287]  [MGI Ref ID J:64055]

Perera PY; Mayadas TN; Takeuchi O; Akira S; Zaks-Zilberman M; Goyert SM; Vogel SN. 2001. CD11b/CD18 acts in concert with CD14 and toll-like receptor (TLR) 4 to elicit full lipopolysaccharide and taxol-inducible gene expression J Immunol 166(1):574-81. [PubMed: 11123339]  [MGI Ref ID J:66435]

Tang T; Rosenkranz A; Assmann KJM; Goodman MJ; Gutierrez-Ramos JC ; Carroll MC ; Cotran RS ; Mayadas TN. 1997. A role for Mac-1 (CDIIb/CD18) in immune complex-stimulated neutrophil function in vivo: Mac-1 deficiency abrogates sustained Fcgamma receptor-dependent neutrophil adhesion and complement-dependent proteinuria in acute glomerulonephritis. J Exp Med 186(11):1853-63. [PubMed: 9382884]  [MGI Ref ID J:44403]

van Spriel AB; Leusen JH; van Egmond M; Dijkman HB; Assmann KJ; Mayadas TN; van de Winkel JG. 2001. Mac-1 (CD11b/CD18) is essential for Fc receptor-mediated neutrophil cytotoxicity and immunologic synapse formation. Blood 97(8):2478-86. [PubMed: 11290613]  [MGI Ref ID J:68805]

Itgamtm1Myd related

Ahn GO; Tseng D; Liao CH; Dorie MJ; Czechowicz A; Brown JM. 2010. Inhibition of Mac-1 (CD11b/CD18) enhances tumor response to radiation by reducing myeloid cell recruitment. Proc Natl Acad Sci U S A 107(18):8363-8. [PubMed: 20404138]  [MGI Ref ID J:160334]

Bai T; Chen CC; Lau LF. 2010. Matricellular protein CCN1 activates a proinflammatory genetic program in murine macrophages. J Immunol 184(6):3223-32. [PubMed: 20164416]  [MGI Ref ID J:160113]

Bai Y; Qian C; Qian L; Ma F; Hou J; Chen Y; Wang Q; Cao X. 2012. Integrin CD11b negatively regulates TLR9-triggered dendritic cell cross-priming by upregulating microRNA-146a. J Immunol 188(11):5293-302. [PubMed: 22551553]  [MGI Ref ID J:188719]

Baudino L; Sardini A; Ruseva MM; Fossati-Jimack L; Cook HT; Scott D; Simpson E; Botto M. 2014. C3 opsonization regulates endocytic handling of apoptotic cells resulting in enhanced T-cell responses to cargo-derived antigens. Proc Natl Acad Sci U S A 111(4):1503-8. [PubMed: 24474777]  [MGI Ref ID J:206648]

Bhat N; Perera PY; Carboni JM; Blanco J; Golenbock DT; Mayadas TN; Vogel SN. 1999. Use of a photoactivatable taxol analogue to identify unique cellular targets in murine macrophages: identification of murine CD18 as a major taxol-binding protein and a role for Mac-1 in taxol-induced gene expression. J Immunol 162(12):7335-42. [PubMed: 10358184]  [MGI Ref ID J:55525]

Bose TO; Pham QM; Jellison ER; Mouries J; Ballantyne CM; Lefrancois L. 2013. CD11a Regulates Effector CD8 T Cell Differentiation and Central Memory Development in Response to Infection with Listeria monocytogenes. Infect Immun 81(4):1140-51. [PubMed: 23357382]  [MGI Ref ID J:194055]

Bourgeois C; Majer O; Frohner IE; Lesiak-Markowicz I; Hildering KS; Glaser W; Stockinger S; Decker T; Akira S; Muller M; Kuchler K. 2011. Conventional Dendritic Cells Mount a Type I IFN Response against Candida spp. Requiring Novel Phagosomal TLR7-Mediated IFN-{beta} Signaling. J Immunol 186(5):3104-12. [PubMed: 21282509]  [MGI Ref ID J:169378]

Brandhorst TT; Wuthrich M; Finkel-Jimenez B; Warner T; Klein BS. 2004. Exploiting type 3 complement receptor for TNF-alpha suppression, immune evasion, and progressive pulmonary fungal infection. J Immunol 173(12):7444-53. [PubMed: 15585870]  [MGI Ref ID J:94857]

Bullard DC; Hu X; Schoeb TR; Axtell RC; Raman C; Barnum SR. 2005. Critical requirement of CD11b (Mac-1) on T cells and accessory cells for development of experimental autoimmune encephalomyelitis. J Immunol 175(10):6327-33. [PubMed: 16272284]  [MGI Ref ID J:119354]

Carter CR; Whitcomb JP; Campbell JA; Mukbel RM; McDowell MA. 2009. Complement receptor 3 deficiency influences lesion progression during Leishmania major infection in BALB/c mice. Infect Immun 77(12):5668-75. [PubMed: 19797068]  [MGI Ref ID J:155481]

Coxon A; Tang T; Mayadas TN. 1999. Cytokine-activated endothelial cells delay neutrophil apoptosis in vitro and in vivo. A role for granulocyte/macrophage colony-stimulating factor. J Exp Med 190(7):923-34. [PubMed: 10510082]  [MGI Ref ID J:115089]

Cramer DE; Allendorf DJ; Baran JT; Hansen R; Marroquin J; Li B; Ratajczak J; Ratajczak MZ; Yan J. 2006. Beta-glucan enhances complement-mediated hematopoietic recovery after bone marrow injury. Blood 107(2):835-40. [PubMed: 16179370]  [MGI Ref ID J:126628]

Dong ZM; Gutierrez-Ramos JC; Coxon A; Mayadas TN; Wagner DD. 1997. A new class of obesity genes encodes leukocyte adhesion receptors. Proc Natl Acad Sci U S A 94(14):7526-30. [PubMed: 9207125]  [MGI Ref ID J:41751]

Evangelista V; Pamuklar Z; Piccoli A; Manarini S; Dell'elba G; Pecce R; Martelli N; Federico L; Rojas M; Berton G; Lowell CA; Totani L; Smyth SS. 2007. Src family kinases mediate neutrophil adhesion to adherent platelets. Blood 109(6):2461-9. [PubMed: 17095622]  [MGI Ref ID J:145358]

Friedrichs K; Adam M; Remane L; Mollenhauer M; Rudolph V; Rudolph TK; Andrie RP; Stockigt F; Schrickel JW; Ravekes T; Deuschl F; Nickenig G; Willems S; Baldus S; Klinke A. 2014. Induction of atrial fibrillation by neutrophils critically depends on CD11b/CD18 integrins. PLoS One 9(2):e89307. [PubMed: 24558493]  [MGI Ref ID J:213347]

Fu H; Liu B; Frost JL; Hong S; Jin M; Ostaszewski B; Shankar GM; Costantino IM; Carroll MC; Mayadas TN; Lemere CA. 2012. Complement component C3 and complement receptor type 3 contribute to the phagocytosis and clearance of fibrillar Abeta by microglia. Glia 60(6):993-1003. [PubMed: 22438044]  [MGI Ref ID J:183868]

Gao HM; Zhou H; Zhang F; Wilson BC; Kam W; Hong JS. 2011. HMGB1 acts on microglia Mac1 to mediate chronic neuroinflammation that drives progressive neurodegeneration. J Neurosci 31(3):1081-92. [PubMed: 21248133]  [MGI Ref ID J:168559]

Geier H; Celli J. 2011. Phagocytic Receptors Dictate Phagosomal Escape and Intracellular Proliferation of Francisella tularensis. Infect Immun 79(6):2204-14. [PubMed: 21422184]  [MGI Ref ID J:171926]

Ghasemzadeh M; Kaplan ZS; Alwis I; Schoenwaelder SM; Ashworth KJ; Westein E; Hosseini E; Salem HH; Slattery R; McColl SR; Hickey MJ; Ruggeri ZM; Yuan Y; Jackson SP. 2013. The CXCR1/2 ligand NAP-2 promotes directed intravascular leukocyte migration through platelet thrombi. Blood 121(22):4555-66. [PubMed: 23550035]  [MGI Ref ID J:198197]

Gil-Bernabe AM; Ferjancic S; Tlalka M; Zhao L; Allen PD; Im JH; Watson K; Hill SA; Amirkhosravi A; Francis JL; Pollard JW; Ruf W; Muschel RJ. 2012. Recruitment of monocytes/macrophages by tissue factor-mediated coagulation is essential for metastatic cell survival and premetastatic niche establishment in mice. Blood 119(13):3164-75. [PubMed: 22327225]  [MGI Ref ID J:182514]

Gorina R; Lyck R; Vestweber D; Engelhardt B. 2014. beta2 integrin-mediated crawling on endothelial ICAM-1 and ICAM-2 is a prerequisite for transcellular neutrophil diapedesis across the inflamed blood-brain barrier. J Immunol 192(1):324-37. [PubMed: 24259506]  [MGI Ref ID J:207099]

Gu C; Jenkins SA; Xue Q; Xu Y. 2012. Activation of the classical complement pathway by Bacillus anthracis is the primary mechanism for spore phagocytosis and involves the spore surface protein BclA. J Immunol 188(9):4421-31. [PubMed: 22442442]  [MGI Ref ID J:188397]

Guerau-de-Arellano M; Alroy J; Bullard D; Huber BT. 2005. Aggravated Lyme carditis in CD11a-/- and CD11c-/- mice. Infect Immun 73(11):7637-43. [PubMed: 16239568]  [MGI Ref ID J:104290]

Guimaraes AJ; Frases S; Pontes B; de Cerqueira MD; Rodrigues ML; Viana NB; Nimrichter L; Nosanchuk JD. 2011. Agglutination of Histoplasma capsulatum by IgG monoclonal antibodies against Hsp60 impacts macrophage effector functions. Infect Immun 79(2):918-27. [PubMed: 21134968]  [MGI Ref ID J:168690]

Hajishengallis G; Shakhatreh MA; Wang M; Liang S. 2007. Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo. J Immunol 179(4):2359-67. [PubMed: 17675497]  [MGI Ref ID J:151219]

Hajishengallis G; Wang M; Bagby GJ; Nelson S. 2008. Importance of TLR2 in early innate immune response to acute pulmonary infection with Porphyromonas gingivalis in mice. J Immunol 181(6):4141-9. [PubMed: 18768871]  [MGI Ref ID J:139089]

Han C; Jin J; Xu S; Liu H; Li N; Cao X. 2010. Integrin CD11b negatively regulates TLR-triggered inflammatory responses by activating Syk and promoting degradation of MyD88 and TRIF via Cbl-b. Nat Immunol 11(8):734-42. [PubMed: 20639876]  [MGI Ref ID J:162535]

Hawley KL; Olson CM Jr; Iglesias-Pedraz JM; Navasa N; Cervantes JL; Caimano MJ; Izadi H; Ingalls RR; Pal U; Salazar JC; Radolf JD; Anguita J. 2012. CD14 cooperates with complement receptor 3 to mediate MyD88-independent phagocytosis of Borrelia burgdorferi. Proc Natl Acad Sci U S A :. [PubMed: 22232682]  [MGI Ref ID J:179916]

Henneke P; Takeuchi O; Malley R; Lien E; Ingalls RR; Freeman MW; Mayadas T; Nizet V; Akira S; Kasper DL; Golenbock DT. 2002. Cellular activation, phagocytosis, and bactericidal activity against group B streptococcus involve parallel myeloid differentiation factor 88-dependent and independent signaling pathways. J Immunol 169(7):3970-7. [PubMed: 12244198]  [MGI Ref ID J:120215]

Hidalgo A; Chang J; Jang JE; Peired AJ; Chiang EY; Frenette PS. 2009. Heterotypic interactions enabled by polarized neutrophil microdomains mediate thromboinflammatory injury. Nat Med 15(4):384-91. [PubMed: 19305412]  [MGI Ref ID J:149370]

Hirahashi J; Hishikawa K; Kaname S; Tsuboi N; Wang Y; Simon DI; Stavrakis G; Shimosawa T; Xiao L; Nagahama Y; Suzuki K; Fujita T; Mayadas TN. 2009. Mac-1 (CD11b/CD18) links inflammation and thrombosis after glomerular injury. Circulation 120(13):1255-65. [PubMed: 19752320]  [MGI Ref ID J:167501]

Hirahashi J; Mekala D; Van Ziffle J; Xiao L; Saffaripour S; Wagner DD; Shapiro SD; Lowell C; Mayadas TN. 2006. Mac-1 signaling via Src-family and Syk kinases results in elastase-dependent thrombohemorrhagic vasculopathy. Immunity 25(2):271-83. [PubMed: 16872848]  [MGI Ref ID J:113463]

Hoffmeister KM; Felbinger TW; Falet H; Denis CV; Bergmeier W; Mayadas TN; von Andrian UH; Wagner DD; Stossel TP; Hartwig JH. 2003. The clearance mechanism of chilled blood platelets. Cell 112(1):87-97. [PubMed: 12526796]  [MGI Ref ID J:107707]

Hong F; Hansen RD; Yan J; Allendorf DJ; Baran JT; Ostroff GR; Ross GD. 2003. Beta-glucan functions as an adjuvant for monoclonal antibody immunotherapy by recruiting tumoricidal granulocytes as killer cells. Cancer Res 63(24):9023-31. [PubMed: 14695221]  [MGI Ref ID J:87069]

Hu C; Mayadas-Norton T; Tanaka K; Chan J; Salgame P. 2000. Mycobacterium tuberculosis infection in complement receptor 3-deficient mice. J Immunol 165(5):2596-602. [PubMed: 10946287]  [MGI Ref ID J:64055]

Hu X; Zhang D; Pang H; Caudle WM; Li Y; Gao H; Liu Y; Qian L; Wilson B; Di Monte DA; Ali SF; Zhang J; Block ML; Hong JS. 2008. Macrophage antigen complex-1 mediates reactive microgliosis and progressive dopaminergic neurodegeneration in the MPTP model of Parkinson's disease. J Immunol 181(10):7194-204. [PubMed: 18981141]  [MGI Ref ID J:140934]

Imai M; Landen C; Ohta R; Cheung NK; Tomlinson S. 2005. Complement-mediated mechanisms in anti-GD2 monoclonal antibody therapy of murine metastatic cancer. Cancer Res 65(22):10562-8. [PubMed: 16288049]  [MGI Ref ID J:103401]

Ip WK; Takahashi K; Moore KJ; Stuart LM; Ezekowitz RA. 2008. Mannose-binding lectin enhances Toll-like receptors 2 and 6 signaling from the phagosome. J Exp Med 205(1):169-81. [PubMed: 18180310]  [MGI Ref ID J:131122]

Ji H; Ohmura K; Mahmood U; Lee DM; Hofhuis FM; Boackle SA; Takahashi K; Holers VM; Walport M; Gerard C; Ezekowitz A; Carroll MC; Brenner M; Weissleder R; Verbeek JS; Duchatelle V; Degott C; Benoist C; Mathis D. 2002. Arthritis critically dependent on innate immune system players. Immunity 16(2):157-68. [PubMed: 11869678]  [MGI Ref ID J:74720]

Kadioglu A; De Filippo K; Bangert M; Fernandes VE; Richards L; Jones K; Andrew PW; Hogg N. 2011. The integrins Mac-1 and alpha4beta1 perform crucial roles in neutrophil and T cell recruitment to lungs during Streptococcus pneumoniae infection. J Immunol 186(10):5907-15. [PubMed: 21460207]  [MGI Ref ID J:173106]

Kim DD; Miwa T; Kimura Y; Schwendener RA; van Lookeren Campagne M; Song WC. 2008. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood 112(4):1109-19. [PubMed: 18524992]  [MGI Ref ID J:138410]

Kirimanjeswara GS; Mann PB; Pilione M; Kennett MJ; Harvill ET. 2005. The complex mechanism of antibody-mediated clearance of Bordetella from the lungs requires TLR4. J Immunol 175(11):7504-11. [PubMed: 16301658]  [MGI Ref ID J:122156]

Kumar S; Xu J; Perkins C; Guo F; Snapper S; Finkelman FD; Zheng Y; Filippi MD. 2012. Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules. Blood 120(17):3563-74. [PubMed: 22932798]  [MGI Ref ID J:192929]

Lammermann T; Afonso PV; Angermann BR; Wang JM; Kastenmuller W; Parent CA; Germain RN. 2013. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature 498(7454):371-5. [PubMed: 23708969]  [MGI Ref ID J:198733]

Li J; Kim K; Hahm E; Molokie R; Hay N; Gordeuk VR; Du X; Cho J. 2014. Neutrophil AKT2 regulates heterotypic cell-cell interactions during vascular inflammation. J Clin Invest 124(4):1483-96. [PubMed: 24642468]  [MGI Ref ID J:209590]

Liu Z; Zhao M; Li N; Diaz LA; Mayadas TN. 2006. Differential roles for beta2 integrins in experimental autoimmune bullous pemphigoid. Blood 107(3):1063-9. [PubMed: 16234355]  [MGI Ref ID J:127587]

Moriyama M; Fukuhara T; Britschgi M; He Y; Narasimhan R; Villeda S; Molina H; Huber BT; Holers M; Wyss-Coray T. 2011. Complement Receptor 2 Is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis. J Neurosci 31(11):3981-3989. [PubMed: 21411641]  [MGI Ref ID J:170456]

Morrison TE; Simmons JD; Heise MT. 2008. Complement receptor 3 promotes severe ross river virus-induced disease. J Virol 82(22):11263-72. [PubMed: 18787004]  [MGI Ref ID J:153394]

Nakazawa T; Nakazawa C; Matsubara A; Noda K; Hisatomi T; She H; Michaud N; Hafezi-Moghadam A; Miller JW; Benowitz LI. 2006. Tumor necrosis factor-alpha mediates oligodendrocyte death and delayed retinal ganglion cell loss in a mouse model of glaucoma. J Neurosci 26(49):12633-41. [PubMed: 17151265]  [MGI Ref ID J:116761]

Paixao-Cavalcante D; Hanson S; Botto M; Cook HT; Pickering MC. 2009. Factor H facilitates the clearance of GBM bound iC3b by controlling C3 activation in fluid phase. Mol Immunol 46(10):1942-50. [PubMed: 19411110]  [MGI Ref ID J:149558]

Paul R; Obermaier B; Van Ziffle J; Angele B; Pfister HW; Lowell CA; Koedel U. 2008. Myeloid Src kinases regulate phagocytosis and oxidative burst in pneumococcal meningitis by activating NADPH oxidase. J Leukoc Biol 84(4):1141-50. [PubMed: 18625913]  [MGI Ref ID J:140230]

Pei Z; Pang H; Qian L; Yang S; Wang T; Zhang W; Wu X; Dallas S; Wilson B; Reece JM; Miller DS; Hong JS; Block ML. 2007. MAC1 mediates LPS-induced production of superoxide by microglia: the role of pattern recognition receptors in dopaminergic neurotoxicity. Glia 55(13):1362-73. [PubMed: 17654704]  [MGI Ref ID J:156303]

Perera PY; Mayadas TN; Takeuchi O; Akira S; Zaks-Zilberman M; Goyert SM; Vogel SN. 2001. CD11b/CD18 acts in concert with CD14 and toll-like receptor (TLR) 4 to elicit full lipopolysaccharide and taxol-inducible gene expression J Immunol 166(1):574-81. [PubMed: 11123339]  [MGI Ref ID J:66435]

Pilione MR; Agosto LM; Kennett MJ; Harvill ET. 2006. CD11b is required for the resolution of inflammation induced by Bordetella bronchiseptica respiratory infection. Cell Microbiol 8(5):758-68. [PubMed: 16611225]  [MGI Ref ID J:135740]

Qing X; Koo GC; Salmon JE. 2012. Complement regulates conventional DC-mediated NK-cell activation by inducing TGF-beta1 in Gr-1+ myeloid cells. Eur J Immunol 42(7):1723-34. [PubMed: 22535677]  [MGI Ref ID J:187776]

Rooyakkers AW; Stokes RW. 2005. Absence of complement receptor 3 results in reduced binding and ingestion of Mycobacterium tuberculosis but has no significant effect on the induction of reactive oxygen and nitrogen intermediates or on the survival of the bacteria in resident and interferon-gamma activated macrophages. Microb Pathog 39(3):57-67. [PubMed: 16084683]  [MGI Ref ID J:106398]

Rosenkranz AR; Coxon A; Maurer M; Gurish MF; Austen KF; Friend DS; Galli SJ; Mayadas TN. 1998. Impaired mast cell development and innate immunity in Mac-1 (CD11b/CD18, CR3)-deficient mice. J Immunol 161(12):6463-7. [PubMed: 9862668]  [MGI Ref ID J:52128]

Rosetti F; Tsuboi N; Chen K; Nishi H; Ernandez T; Sethi S; Croce K; Stavrakis G; Alcocer-Varela J; Gomez-Martin D; van Rooijen N; Kyttaris VC; Lichtman AH; Tsokos GC; Mayadas TN. 2012. Human lupus serum induces neutrophil-mediated organ damage in mice that is enabled by Mac-1 deficiency. J Immunol 189(7):3714-23. [PubMed: 22933624]  [MGI Ref ID J:190352]

Scott MJ; Billiar TR. 2008. Beta2-integrin-induced p38 MAPK activation is a key mediator in the CD14/TLR4/MD2-dependent uptake of lipopolysaccharide by hepatocytes. J Biol Chem 283(43):29433-46. [PubMed: 18701460]  [MGI Ref ID J:142561]

Segura E; Guerin C; Hogg N; Amigorena S; Thery C. 2007. CD8+ dendritic cells use LFA-1 to capture MHC-peptide complexes from exosomes in vivo. J Immunol 179(3):1489-96. [PubMed: 17641014]  [MGI Ref ID J:149952]

Sisco M; Chao JD; Kim I; Mogford JE; Mayadas TN; Mustoe TA. 2007. Delayed wound healing in Mac-1-deficient mice is associated with normal monocyte recruitment. Wound Repair Regen 15(4):566-71. [PubMed: 17650101]  [MGI Ref ID J:147686]

Soriano SG; Coxon A; Wang YF; Frosch MP; Lipton SA; Hickey PR; Mayadas TN. 1999. Mice deficient in Mac-1 (CD11b/CD18) are less susceptible to cerebral ischemia/reperfusion injury. Stroke 30(1):134-9. [PubMed: 9880401]  [MGI Ref ID J:103873]

Szczur K; Zheng Y; Filippi MD. 2009. The small Rho GTPase Cdc42 regulates neutrophil polarity via CD11b integrin signaling. Blood 114(20):4527-37. [PubMed: 19752396]  [MGI Ref ID J:154928]

Tang T; Rosenkranz A; Assmann KJM; Goodman MJ; Gutierrez-Ramos JC ; Carroll MC ; Cotran RS ; Mayadas TN. 1997. A role for Mac-1 (CDIIb/CD18) in immune complex-stimulated neutrophil function in vivo: Mac-1 deficiency abrogates sustained Fcgamma receptor-dependent neutrophil adhesion and complement-dependent proteinuria in acute glomerulonephritis. J Exp Med 186(11):1853-63. [PubMed: 9382884]  [MGI Ref ID J:44403]

Taylor PR; Roy S; Leal SM Jr; Sun Y; Howell SJ; Cobb BA; Li X; Pearlman E. 2014. Activation of neutrophils by autocrine IL-17A-IL-17RC interactions during fungal infection is regulated by IL-6, IL-23, RORgammat and dectin-2. Nat Immunol 15(2):143-51. [PubMed: 24362892]  [MGI Ref ID J:209279]

Utomo A; Hirahashi J; Mekala D; Asano K; Glogauer M; Cullere X; Mayadas TN. 2008. Requirement for Vav proteins in post-recruitment neutrophil cytotoxicity in IgG but not complement C3-dependent injury. J Immunol 180(9):6279-87. [PubMed: 18424751]  [MGI Ref ID J:134524]

Van Ziffle JA; Lowell CA. 2009. Neutrophil-specific deletion of Syk kinase results in reduced host defense to bacterial infection. Blood 114(23):4871-82. [PubMed: 19797524]  [MGI Ref ID J:155479]

Vander Lugt B; Beck ZT; Fuhlbrigge RC; Hacohen N; Campbell JJ; Boes M. 2011. TGF-beta Suppresses beta-Catenin-Dependent Tolerogenic Activation Program in Dendritic Cells. PLoS One 6(5):e20099. [PubMed: 21625453]  [MGI Ref ID J:172583]

Wakselman S; Bechade C; Roumier A; Bernard D; Triller A; Bessis A. 2008. Developmental neuronal death in hippocampus requires the microglial CD11b integrin and DAP12 immunoreceptor. J Neurosci 28(32):8138-43. [PubMed: 18685038]  [MGI Ref ID J:140167]

Wolfe DN; Kirimanjeswara GS; Harvill ET. 2005. Clearance of Bordetella parapertussis from the lower respiratory tract requires humoral and cellular immunity. Infect Immun 73(10):6508-13. [PubMed: 16177324]  [MGI Ref ID J:104212]

Yamamoto S; Nava RG; Zhu J; Huang HJ; Ibrahim M; Mohanakumar T; Miller MJ; Krupnick AS; Kreisel D; Gelman AE. 2012. Cutting edge: Pseudomonas aeruginosa abolishes established lung transplant tolerance by stimulating B7 expression on neutrophils. J Immunol 189(9):4221-5. [PubMed: 23018463]  [MGI Ref ID J:190610]

Yan J; Vetvicka V; Xia Y; Coxon A; Carroll MC; Mayadas TN; Ross GD. 1999. Beta-glucan, a 'specific' biologic response modifier that uses antibodies to target tumors for cytotoxic recognition by leukocyte complement receptor type 3 (CD11b/CD18). J Immunol 163(6):3045-52. [PubMed: 10477568]  [MGI Ref ID J:57602]

Zaltzman R; Alexandrovich A; Trembovler V; Shohami E; Gozes I. 2005. The influence of the peptide NAP on Mac-1-deficient mice following closed head injury. Peptides 26(8):1520-7. [PubMed: 16042992]  [MGI Ref ID J:106672]

Zhang B; Hirahashi J; Cullere X; Mayadas TN. 2003. Elucidation of molecular events leading to neutrophil apoptosis following phagocytosis: cross-talk between caspase 8, reactive oxygen species, and MAPK/ERK activation. J Biol Chem 278(31):28443-54. [PubMed: 12736263]  [MGI Ref ID J:120441]

Zhang W; Dallas S; Zhang D; Guo JP; Pang H; Wilson B; Miller DS; Chen B; Zhang W; McGeer PL; Hong JS; Zhang J. 2007. Microglial PHOX and Mac-1 are essential to the enhanced dopaminergic neurodegeneration elicited by A30P and A53T mutant alpha-synuclein. Glia 55(11):1178-88. [PubMed: 17600340]  [MGI Ref ID J:156306]

Zhou H; Liao J; Aloor J; Nie H; Wilson BC; Fessler MB; Gao HM; Hong JS. 2013. CD11b/CD18 (Mac-1) Is a Novel Surface Receptor for Extracellular Double-Stranded RNA To Mediate Cellular Inflammatory Responses. J Immunol 190(1):115-25. [PubMed: 23209319]  [MGI Ref ID J:190826]

van Spriel AB; Leusen JH; van Egmond M; Dijkman HB; Assmann KJ; Mayadas TN; van de Winkel JG. 2001. Mac-1 (CD11b/CD18) is essential for Fc receptor-mediated neutrophil cytotoxicity and immunologic synapse formation. Blood 97(8):2478-86. [PubMed: 11290613]  [MGI Ref ID J:68805]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129S4 background and has been backcrossed to C57BL/6 for at least ten generations. When maintaining a homozygous colony, the donating investigator advises replacing breeders every six months with the offspring from heterozygous matings, avoiding sibling matings. Coat color expected from breeding:Black
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K20

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Itgamtm1Myd  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Itgamtm1Myd x Homozygous for Itgamtm1Myd  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Itgamtm1Myd  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Itgamtm1Myd x Homozygous for Itgamtm1Myd  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use

Terms of Use


General Terms and Conditions


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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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