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Strain Name:

B6.129S4-Zp3tm1Dean/J

Stock Number:

004038

Availability:

Repository-Cryopreserved


General Terms and Conditions

Genes & Alleles   Zp3;   Zp3tm1Dean;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Donating Investigator Jurrien Dean,   NIH
GenerationN3

Strain Description
Mice that are homozygous null for the Zp3 gene are viable, normal in size and do not display any gross physical or behavioral abnormalities. No Zp3 gene product (mRNA or protein) is detected. Homozygous females are not fertile; they lack a zona pellucida and exhibit a disorganized corona radiata. Heterozygous females are fertile. Homozygous males appear normal in all respects and are fertile.

Strain Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the Zp3 exon 1. The construct was transfected into 129S4/SvJae-derived J1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6N blastocysts. The resulting chimeric animals were backcrossed to C57BL/6N mice.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Zp3tm1Dean/Zp3tm1Dean

        involves: 129S4/SvJae * C57BL/6J
  • endocrine/exocrine gland phenotype
  • abnormal ovarian folliculogenesis (MGI Ref ID J:36772)
    • disorganized corona radiata
    • abnormal cumulus-oocyte complex allowing oocyte to dissociate from the cumulus mass
  • reproductive system phenotype
  • abnormal ovarian folliculogenesis (MGI Ref ID J:36772)
    • disorganized corona radiata
    • abnormal cumulus-oocyte complex allowing oocyte to dissociate from the cumulus mass
  • abnormal ovulation (MGI Ref ID J:36772)
    • few eggs were recovered after induced ovulation, relative to wild type
    • recovered eggs were unfertilized
  • absent zona pellucida (MGI Ref ID J:36772)
    • oocytes lacked the zona pellucida matrix
  • female infertility (MGI Ref ID J:36772)

Gene & Allele Details

Allele Symbol Zp3tm1Dean
Allele Name targeted mutation 1, Jurrien Dean
Common Name(s) Zp3tm1Tra;
Mutation Made By Jurrien Dean,   NIH
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Zp3, zona pellucida glycoprotein 3
Chromosome 5
Gene Common Name(s) ZP3A; ZP3B; ZPC; Zp-3;
Molecular Note A neomycin cassette was inserted into exon 1. In situ hybridization experiments demonstrated an absence of mRNA in oocytes and the protein was undetectable in immunofluorescence experiments on mutant ovaries and oocytes. [MGI Ref ID J:36772]

Control Information

  Allele   Control
 Zp3tm1Dean  Wild-type from the colony
 
  Considerations for Choosing Controls

Genotyping Protocols

NEOTD (Generic Neo)

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129 background and has been backcrossed to C57BL/6N for at least ten generations. It is maintained by breeding homozygote males to wildtype females. Coat color expected from breeding:Black

Related Strains

Strains carrying other alleles of Zp3
006888   C57BL/6-Tg(Zp3-cre)1Gwh/J
003394   C57BL/6-Tg(Zp3-cre)3Mrt/J
003651   C57BL/6-Tg(Zp3-cre)93Knw/J
003377   FVB/N-Tg(Zp3-cre)3Mrt/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
View Strains carrying other alleles of Zp3     (5 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Zp3tm1Dean related

Developmental Biology Research

Reproductive Biology Research
Fertility Defects (females only)

References

Selected Reference(s)

Rankin T; Familari M; Lee E; Ginsberg A; Dwyer N; Blanchette-Mackie J; Drago J; Westphal H; Dean J. 1996. Mice homozygous for an insertional mutation in the Zp3 gene lack a zona pellucida and are infertile. Development 122(9):2903-10. [PubMed: 8787763]  [MGI Ref ID J:36772]

Additional References

Price and Supply Information

Strain Name: B6.129S4-Zp3tm1Dean/J
Stock Number: 004038

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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