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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N3
Generation DefinitionsDonating Investigator Dr. Jurrien Dean, NIH Description
Mice that are homozygous null for the Zp3 gene are viable, normal in size and do not display any gross physical or behavioral abnormalities. No Zp3 gene product (mRNA or protein) is detected. Homozygous females are not fertile; they lack a zona pellucida and exhibit a disorganized corona radiata. Heterozygous females are fertile. Homozygous males appear normal in all respects and are fertile.Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the Zp3 exon 1. The construct was transfected into 129S4/SvJae-derived J1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6N blastocysts. The resulting chimeric animals were backcrossed to C57BL/6N mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Zp3
003394 B6.FVB-Tg(Zp3-cre)3Mrt/J 006888 C57BL/6-Tg(Zp3-cre)1Gwh/J 003651 C57BL/6-Tg(Zp3-cre)93Knw/J 003650 C57BL/6J-Tg(Zp3-cre)82Knw/KnwJ 003377 FVB/N-Tg(Zp3-cre)3Mrt/J 006129 STOCK Tg(Zp3-EGFP)1Dean/J View Strains carrying other alleles of Zp3 (6 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Zp3tm1Dean/Zp3tm1Dean
involves: 129S4/SvJae * C57BL/6JThis mouse can be used to support research in many areas including:Zp3tm1Dean related
Developmental Biology Research
Reproductive Biology Research
Fertility Defects
females only
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Zp3tm1Dean | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jurrien Dean | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Zp3tm1Tra; | ||
| Mutation Made By | Dr. Jurrien Dean, NIH | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Zp3, zona pellucida glycoprotein 3 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | ZP3A; ZP3B; ZPC; Zp-3; | ||
| Molecular Note | A neomycin cassette was inserted into exon 1. In situ hybridization experiments demonstrated an absence of mRNA in oocytes and the protein was undetectable in immunofluorescence experiments on mutant ovaries and oocytes. [MGI Ref ID J:36772] | ||
Genotyping
Genotyping Information
Genotyping Protocols
NEOTD (Generic Neo), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Rankin T; Familari M; Lee E; Ginsberg A; Dwyer N; Blanchette-Mackie J; Drago J; Westphal H; Dean J. 1996. Mice homozygous for an insertional mutation in the Zp3 gene lack a zona pellucida and are infertile. Development 122(9):2903-10. [PubMed: 8787763] [MGI Ref ID J:36772]
Additional References
Zp3tm1Dean relatedChoi Y; Rajkovic A. 2006. Genetics of early mammalian folliculogenesis. Cell Mol Life Sci 63(5):579-90. [PubMed: 16416028] [MGI Ref ID J:108336]
Gahlay G; Gauthier L; Baibakov B; Epifano O; Dean J. 2010. Gamete recognition in mice depends on the cleavage status of an egg's zona pellucida protein. Science 329(5988):216-9. [PubMed: 20616279] [MGI Ref ID J:161507]
Hoodbhoy T; Aviles M; Baibakov B; Epifano O; Jimenez-Movilla M; Gauthier L; Dean J. 2006. ZP2 and ZP3 traffic independently within oocytes prior to assembly into the extracellular zona pellucida. Mol Cell Biol 26(21):7991-8. [PubMed: 17047254] [MGI Ref ID J:114998]
Naz RK; Rajesh C. 2005. Gene knockouts that cause female infertility: search for novel contraceptive targets Front Biosci 10:2447-2459. [PubMed: 15970507] [MGI Ref ID J:103183]
Rankin TL; Coleman JS; Epifano O; Hoodbhoy T; Turner SG; Castle PE; Lee E; Gore-Langton R; Dean J. 2003. Fertility and taxon-specific sperm binding persist after replacement of mouse sperm receptors with human homologs. Dev Cell 5(1):33-43. [PubMed: 12852850] [MGI Ref ID J:93482]
Rankin TL; Tong ZB; Castle PE; Lee E; Gore-Langton R; Nelson LM; Dean J. 1998. Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125(13):2415-24. [PubMed: 9609824] [MGI Ref ID J:77459]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129 background and has been backcrossed to C57BL/6N for at least ten generations. It is maintained by breeding homozygote males to wildtype females. Coat color expected from breeding:Black
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3000.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.