Strain Name: |
B6.129S4-Zp3tm1Dean/J |
|---|---|
Stock Number: |
004038 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Zp3; Zp3tm1Dean; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Jurrien Dean, NIH Generation N3 Strain Description
Mice that are homozygous null for the Zp3 gene are viable, normal in size and do not display any gross physical or behavioral abnormalities. No Zp3 gene product (mRNA or protein) is detected. Homozygous females are not fertile; they lack a zona pellucida and exhibit a disorganized corona radiata. Heterozygous females are fertile. Homozygous males appear normal in all respects and are fertile.Strain Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the Zp3 exon 1. The construct was transfected into 129S4/SvJae-derived J1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6N blastocysts. The resulting chimeric animals were backcrossed to C57BL/6N mice.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Zp3tm1Dean | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jurrien Dean | ||
| Common Name(s) | Zp3tm1Tra; | ||
| Mutation Made By | Jurrien Dean, NIH | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Zp3, zona pellucida glycoprotein 3 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | ZP3A; ZP3B; ZPC; Zp-3; | ||
| Molecular Note | A neomycin cassette was inserted into exon 1. In situ hybridization experiments demonstrated an absence of mRNA in oocytes and the protein was undetectable in immunofluorescence experiments on mutant ovaries and oocytes. [MGI Ref ID J:36772] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Zp3tm1Dean | Wild-type from the colony | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
NEOTD (Generic Neo)
Colony Maintenance
| Breeding & Husbandry | This strain originated on a B6;129 background and has been backcrossed to C57BL/6N for at least ten generations. It is maintained by breeding homozygote males to wildtype females. Coat color expected from breeding:Black |
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Related Strains
Strains carrying other alleles of Zp3
006888 C57BL/6-Tg(Zp3-cre)1Gwh/J 003394 C57BL/6-Tg(Zp3-cre)3Mrt/J 003651 C57BL/6-Tg(Zp3-cre)93Knw/J 003377 FVB/N-Tg(Zp3-cre)3Mrt/J 006129 STOCK Tg(Zp3-EGFP)1Dean/J View Strains carrying other alleles of Zp3 (5 strains)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:Zp3tm1Dean related
Developmental Biology Research
Reproductive Biology Research
Fertility Defects (females only)
References
Selected Reference(s)
Additional ReferencesRankin T; Familari M; Lee E; Ginsberg A; Dwyer N; Blanchette-Mackie J; Drago J; Westphal H; Dean J. 1996. Mice homozygous for an insertional mutation in the Zp3 gene lack a zona pellucida and are infertile. Development 122(9):2903-10. [PubMed: 8787763] [MGI Ref ID J:36772]
Price and Supply Information
| Strain Name: | B6.129S4-Zp3tm1Dean/J |
| Stock Number: | 004038 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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