Description

Strain Information

Former Names FVB;129S-Men1tm1Ctre    (Changed: 15-DEC-04 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Francis Collins,   NHGRI/NIH

Description
Mice that are homozygous null for the Men1 gene die in utero at embryonic days 10.5-11.5, exhibiting delayed development often (20%) with defects in cranial/facial formation. At birth, heterozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. At nine months, ~80% of the heterozygous-null mice develop abnormalities in pancreatic islet cells, the severity of which ranges from hyperplasia to insulin-producing tumors. Parathyroid adenomas are also observed at this age. Tumor incidence is progressive, with occurrences in multiple endocrine tissues (pancreatic islets, parathyroids, thyroid, adrenal cortex, pituitary) by sixteen months of age.

Development
A targeting vector containing a loxP-bracketed neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter was inserted into intron 2 of the Men1 gene. Another loxP site was positioned within intron 8. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were bred to 129S6/SvEvTacFBR females. The progeny were heterozygous for the floxed allele (TSM/+). These heterozygous mice were bred with a ubiquitously expressing Cre mouse line (EIIa-Cre) on an FVB background. The resulting mice were heterozygous for an allele that lacked the PGK-Neo cassette and Men1 exons 3-8.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Men1

005109

129S(FVB)-Men1tm1.2Ctre/J

View Strains carrying other alleles of Men1     (1 strain)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Men1^tm1.1Ctre related

Cancer Research
Increased Tumor Incidence (Adenomas: pancreatic b cells)
Increased Tumor Incidence (Other Tissues/Organs: pituitary)
Tumor Suppressor Genes

Diabetes and Obesity Research
Pancreatic B Cell Adenomas

Mouse/Human Gene Homologs
multiple endocrine neoplasia, type I

Research Tools
Developmental Biology Research (Cre-lox System)
Endocrine Deficiency Research

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol		Men1tm1.1Ctre
Allele Name		targeted mutation 1.1, Judy S Crabtree
Allele Type		Targeted (knock-out)
Common Name(s)		Men1-; Men1deltaN3-8; Men1deltaN3-8;
Mutation Made By		Judy Crabtree,   Wyeth Pharmaceuticals
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		TC-1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Men1, multiple endocrine neoplasia 1
Chromosome		19
Gene Common Name(s)		AW045611; MEAI; MGC114329; SCG2; expressed sequence AW045611; menin;
Molecular Note		This allele is a derivative of Men1tm1Ctre in which the neomycin cassette and exons 3-8 of the gene were removed by Cre mediated recombination by crossing mice carrying the Mentm1Ctre allele to mice expressing Cre recombinase under the control of a ubiquitous promoter. [MGI Ref ID J:67128]

Genotyping

Genotyping Information

Genotyping Protocols

Men1^tm1.1Ctre, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Crabtree JS; Scacheri PC; Ward JM; Garrett-Beal L; Emmert-Buck MR; Edgemon KA; Lorang D; Libutti SK; Chandrasekharappa SC; Marx SJ; Spiegel AM; Collins FS. 2001. A mouse model of multiple endocrine neoplasia, type 1, develops multiple endocrine tumors. Proc Natl Acad Sci U S A 98(3):1118-23. [PubMed: 11158604]  [MGI Ref ID J:67128]

Additional References

Men1^tm1.1Ctre related

Bai F; Pei XH; Nishikawa T; Smith MD; Xiong Y. 2007. p18Ink4c, but not p27Kip1, collaborates with Men1 to suppress neuroendocrine organ tumors. Mol Cell Biol 27(4):1495-504. [PubMed: 17145768]  [MGI Ref ID J:118265]

Donangelo I; Melmed S. 2005. Pathophysiology of pituitary adenomas. J Endocrinol Invest 28(11 Suppl):100-5. [PubMed: 16625857]  [MGI Ref ID J:116408]

La P; Schnepp RW; D Petersen C; C Silva A; Hua X. 2004. Tumor suppressor menin regulates expression of insulin-like growth factor binding protein 2. Endocrinology 145(7):3443-50. [PubMed: 15044367]  [MGI Ref ID J:105625]

Matoso A; Zhou Z; Hayama R; Flesken-Nikitin A; Nikitin AY. 2008. Cell lineage-specific interactions between Men1 and Rb in neuroendocrine neoplasia. Carcinogenesis 29(3):620-8. [PubMed: 17893233]  [MGI Ref ID J:133299]

Pei XH; Bai F; Smith MD; Xiong Y. 2007. p18Ink4c collaborates with Men1 to constrain lung stem cell expansion and suppress non-small-cell lung cancers. Cancer Res 67(7):3162-70. [PubMed: 17409423]  [MGI Ref ID J:120835]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Use restrictions apply, see Policy on Licensing and Use Restrictions.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
OncoMouse^® requires a license from DuPont, see Licenses for Strains with OncoMouse^® Technology.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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