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- Description
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Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation F?+17 (21-JAN-08) Donating Investigator Stuart Orkin, Harvard Medical School Description
These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that the EGFP expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications.Development
A targeting vector containing the mouse phosphoglycerate kinase promoter driving cytosine deaminase and puromycin genes, a STOP fragment, an EGFP cDNA and a Bgeo gene (not expressed), was inserted into the Gt(ROSA)26Sor locus. Located immediately 5' of the puromycin gene, the stop fragment, and the EGFP cDNA are loxP sites. A herpes simplex viral thymidine kinase cassette was used as a negative selection marker. The construct was electroporated into ROSA26 ES cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
Related Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm2Sho | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Stuart Orkin | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | ROSA-GFP; ROSA26-EGFP; ROSA26-EGFTf; Rosa26R-eGFP; RosaC; | ||
| Mutation Made By | Xiaohong Mao, Novartis | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, Enhanced Green Fluorescent Protein expression is observed in the cre-expressing tissues | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in Cre-expressing tissues. | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm2Sho, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Mao X; Fujiwara Y; Chapdelaine A; Yang H; Orkin SH. 2001. Activation of EGFP expression by cre-mediated excision in a new ROSA26 reporter mouse strain Blood 97(1):324-6. [PubMed: 11133778] [MGI Ref ID J:66742]
Additional References
Ohyama T; Groves AK. 2004. Generation of Pax2-Cre mice by modification of a Pax2 bacterial artificial chromosome. Genesis 38(4):195-9. [PubMed: 15083520] [MGI Ref ID J:89784]
Gt(ROSA)26Sortm2Sho relatedBogunovic M; Ginhoux F; Helft J; Shang L; Hashimoto D; Greter M; Liu K; Jakubzick C; Ingersoll MA; Leboeuf M; Stanley ER; Nussenzweig M; Lira SA; Randolph GJ; Merad M. 2009. Origin of the lamina propria dendritic cell network. Immunity 31(3):513-25. [PubMed: 19733489] [MGI Ref ID J:152688]
Charles MA; Mortensen AH; Potok MA; Camper SA. 2008. Pitx2 deletion in pituitary gonadotropes is compatible with gonadal development, puberty, and fertility. Genesis 46(10):507-14. [PubMed: 18802953] [MGI Ref ID J:142726]
Duggan CD; Demaria S; Baudhuin A; Stafford D; Ngai J. 2008. Foxg1 is required for development of the vertebrate olfactory system. J Neurosci 28(20):5229-39. [PubMed: 18480279] [MGI Ref ID J:136319]
Eberl G; Littman DR. 2004. Thymic origin of intestinal alphabeta T Cells Revealed by Fate Mapping of RORgammat+ Cells. Science 305(5681):248-51. [PubMed: 15247480] [MGI Ref ID J:91566]
Fogarty M; Grist M; Gelman D; Marin O; Pachnis V; Kessaris N. 2007. Spatial genetic patterning of the embryonic neuroepithelium generates GABAergic interneuron diversity in the adult cortex. J Neurosci 27(41):10935-46. [PubMed: 17928435] [MGI Ref ID J:125700]
Ghiaur G; Ferkowicz MJ; Milsom MD; Bailey J; Witte D; Cancelas JA; Yoder MC; Williams DA. 2008. Rac1 is essential for intraembryonic hematopoiesis and for the initial seeding of fetal liver with definitive hematopoietic progenitor cells. Blood 111(7):3313-21. [PubMed: 18083846] [MGI Ref ID J:133470]
Gong L; Yao F; Hockman K; Heng HH; Morton GJ; Takeda K; Akira S; Low MJ; Rubinstein M; MacKenzie RG. 2008. Signal transducer and activator of transcription-3 is required in hypothalamic agouti-related protein/neuropeptide Y neurons for normal energy homeostasis. Endocrinology 149(7):3346-54. [PubMed: 18403487] [MGI Ref ID J:145370]
High FA; Zhang M; Proweller A; Tu L; Parmacek MS; Pear WS; Epstein JA. 2007. An essential role for Notch in neural crest during cardiovascular development and smooth muscle differentiation. J Clin Invest 117(2):353-363. [PubMed: 17273555] [MGI Ref ID J:118039]
Hill JW; Williams KW; Ye C; Luo J; Balthasar N; Coppari R; Cowley MA; Cantley LC; Lowell BB; Elmquist JK. 2008. Acute effects of leptin require PI3K signaling in hypothalamic proopiomelanocortin neurons in mice. J Clin Invest 118(5):1796-805. [PubMed: 18382766] [MGI Ref ID J:136147]
Jakubzick C; Bogunovic M; Bonito AJ; Kuan EL; Merad M; Randolph GJ. 2008. Lymph-migrating, tissue-derived dendritic cells are minor constituents within steady-state lymph nodes. J Exp Med 205(12):2839-50. [PubMed: 18981237] [MGI Ref ID J:143220]
Kaplan DH; Li MO; Jenison MC; Shlomchik WD; Flavell RA; Shlomchik MJ. 2007. Autocrine/paracrine TGF{beta}1 is required for the development of epidermal Langerhans cells. J Exp Med 204(11):2545-52. [PubMed: 17938236] [MGI Ref ID J:126147]
Kessaris N; Fogarty M; Iannarelli P; Grist M; Wegner M; Richardson WD. 2006. Competing waves of oligodendrocytes in the forebrain and postnatal elimination of an embryonic lineage. Nat Neurosci 9(2):173-9. [PubMed: 16388308] [MGI Ref ID J:105334]
Leinninger GM; Jo YH; Leshan RL; Louis GW; Yang H; Barrera JG; Wilson H; Opland DM; Faouzi MA; Gong Y; Jones JC; Rhodes CJ; Chua S Jr; Diano S; Horvath TL; Seeley RJ; Becker JB; Munzberg H; Myers MG Jr. 2009. Leptin acts via leptin receptor-expressing lateral hypothalamic neurons to modulate the mesolimbic dopamine system and suppress feeding. Cell Metab 10(2):89-98. [PubMed: 19656487] [MGI Ref ID J:151971]
Leshan RL; Louis GW; Jo YH; Rhodes CJ; Munzberg H; Myers MG Jr. 2009. Direct innervation of GnRH neurons by metabolic- and sexual odorant-sensing leptin receptor neurons in the hypothalamic ventral premammillary nucleus. J Neurosci 29(10):3138-47. [PubMed: 19279251] [MGI Ref ID J:147070]
Liao CP; Zhong C; Saribekyan G; Bading J; Park R; Conti PS; Moats R; Berns A; Shi W; Zhou Z; Nikitin AY; Roy-Burman P. 2007. Mouse models of prostate adenocarcinoma with the capacity to monitor spontaneous carcinogenesis by bioluminescence or fluorescence. Cancer Res 67(15):7525-33. [PubMed: 17671224] [MGI Ref ID J:123911]
Luche H; Weber O; Nageswara Rao T; Blum C; Fehling HJ. 2007. Faithful activation of an extra-bright red fluorescent protein in 'knock-in' Cre-reporter mice ideally suited for lineage tracing studies. Eur J Immunol 37(1):43-53. [PubMed: 17171761] [MGI Ref ID J:117041]
Matsuki T; Nomiyama M; Takahira H; Hirashima N; Kunita S; Takahashi S; Yagami K; Kilduff TS; Bettler B; Yanagisawa M; Sakurai T. 2009. Selective loss of GABA(B) receptors in orexin-producing neurons results in disrupted sleep/wakefulness architecture. Proc Natl Acad Sci U S A 106(11):4459-64. [PubMed: 19246384] [MGI Ref ID J:146778]
Murray SA; Oram KF; Gridley T. 2007. Multiple functions of Snail family genes during palate development in mice. Development 134(9):1789-97. [PubMed: 17376812] [MGI Ref ID J:121243]
Opavsky R; Wang SH; Trikha P; Raval A; Huang Y; Wu YZ; Rodriguez B; Keller B; Liyanarachchi S; Wei G; Davuluri RV; Weinstein M; Felsher D; Ostrowski M; Leone G; Plass C. 2007. CpG island methylation in a mouse model of lymphoma is driven by the genetic configuration of tumor cells. PLoS Genet 3(9):1757-69. [PubMed: 17907813] [MGI Ref ID J:130029]
Schaefer A; O'Carroll D; Tan CL; Hillman D; Sugimori M; Llinas R; Greengard P. 2007. Cerebellar neurodegeneration in the absence of microRNAs. J Exp Med 204(7):1553-8. [PubMed: 17606634] [MGI Ref ID J:125878]
Tavazoie SF; Alvarez VA; Ridenour DA; Kwiatkowski DJ; Sabatini BL. 2005. Regulation of neuronal morphology and function by the tumor suppressors Tsc1 and Tsc2. Nat Neurosci 8(12):1727-34. [PubMed: 16286931] [MGI Ref ID J:103933]
Vidali L; Chen F; Cicchetti G; Ohta Y; Kwiatkowski DJ. 2006. Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor. Mol Biol Cell 17(5):2377-90. [PubMed: 16525021] [MGI Ref ID J:112526]
Yang ZJ; Ellis T; Markant SL; Read TA; Kessler JD; Bourboulas M; Schuller U; Machold R; Fishell G; Rowitch DH; Wainwright BJ; Wechsler-Reya RJ. 2008. Medulloblastoma can be initiated by deletion of Patched in lineage-restricted progenitors or stem cells. Cancer Cell 14(2):135-45. [PubMed: 18691548] [MGI Ref ID J:139573]
Yi L; Domyan ET; Lewandoski M; Sun X. 2008. Fibroblast growth factor 9 signaling inhibits airway smooth muscle differentiation in mouse lung. Dev Dyn 238(1):123-137. [PubMed: 19097117] [MGI Ref ID J:142646]
Yuan TL; Choi HS; Matsui A; Benes C; Lifshits E; Luo J; Frangioni JV; Cantley LC. 2008. Class 1A PI3K regulates vessel integrity during development and tumorigenesis. Proc Natl Acad Sci U S A 105(28):9739-44. [PubMed: 18621722] [MGI Ref ID J:137878]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB27
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129 background and can be maintained as a hemizygote or homozygote. Coat color expected from breeding:Agouti,Black and Dark Agouti Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $97.10 Female or Male Homozygous for Gt(ROSA)26Sortm2Sho
Pairs /Price (US dollars $) Pair Genotype $194.20 Homozygous for Gt(ROSA)26Sortm2Sho x Homozygous for Gt(ROSA)26Sortm2Sho
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $126.30 Female or Male Homozygous for Gt(ROSA)26Sortm2Sho
Pairs /Price (US dollars $) Pair Genotype $252.50 Homozygous for Gt(ROSA)26Sortm2Sho x Homozygous for Gt(ROSA)26Sortm2Sho
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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