Strain Name: |
B6;129-Gt(ROSA)26Sortm2Sho/J |
|---|---|
Stock Number: |
004077 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; Gt(ROSA)26Sor; Gt(ROSA)26Sortm2Sho; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Stuart Orkin, Harvard Medical School Generation F?+17 (21-JAN-08) Strain Description
These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that the EGFP expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications.Strain Development
A targeting vector containing the mouse phosphoglycerate kinase promoter driving cytosine deaminase and puromycin genes, a STOP fragment, an EGFP cDNA and a Bgeo gene (not expressed), was inserted into the Gt(ROSA)26Sor locus. Located immediately 5' of the puromycin gene, the stop fragment, and the EGFP cDNA are loxP sites. A herpes simplex viral thymidine kinase cassette was used as a negative selection marker. The construct was electroporated into ROSA26 ES cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sortm2Sho | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Stuart Orkin | ||
| Common Name(s) | ROSA-GFP; ROSA26-EGFP; ROSA26-EGFTf; Rosa26R-eGFP; RosaC; | ||
| Mutation Made By | Xiaohong Mao, Novartis | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, Enhanced Green Fluorescent Protein expression is observed in the cre-expressing tissues | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in Cre-expressing tissues. | ||
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gt(ROSA)26Sortm2Sho
Colony Maintenance
| Breeding & Husbandry | This strain originated on a B6;129 background and can be maintained as a hemizygote or homozygote. Coat color expected from breeding:Agouti,Black and Dark Agouti |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX Notes, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Animal Health Reports
Room Number FGB27
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox-System (loxP-flanked Sequences: Test/Reporter)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Genetics Research (Tissue/Cell Markers: Cre-lox system)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesMao X; Fujiwara Y; Chapdelaine A; Yang H; Orkin SH. 2001. Activation of EGFP expression by cre-mediated excision in a new ROSA26 reporter mouse strain Blood 97(1):324-6. [PubMed: 11133778] [J:66742]
Price and Supply Information
| Strain Name: | B6;129-Gt(ROSA)26Sortm2Sho/J |
| Stock Number: | 004077 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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