| |||||||||||||||
| These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. The expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation F?+27 (05-MAR-13)
Generation DefinitionsDonating Investigator Dr. Stuart Orkin, Harvard Medical School Description
These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in cre-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator indicates that the EGFP expression level in this reporter strain is suitable for applications involving FACS but is too low for histological applications.Development
A targeting vector containing the mouse phosphoglycerate kinase promoter driving cytosine deaminase and puromycin genes, a STOP fragment, an EGFP cDNA and a Bgeo gene (not expressed), was inserted into the Gt(ROSA)26Sor locus. Located immediately 5' of the puromycin gene, the stop fragment, and the EGFP cDNA are loxP sites. A herpes simplex viral thymidine kinase cassette was used as a negative selection marker. The construct was electroporated into ROSA26 ES cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm2Sho | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Stuart Orkin | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | R26-GFP; R26floxGFP; ROSA-GFP; ROSA26-EGFP; ROSA26-EGFTf; ROSA26-flox-STOP-flox-GFP; Rosa26LOXPEGFP; Rosa26R-eGFP; RosaC; | ||
| Mutation Made By | Dr. Xiaohong Mao, Novartis | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, Enhanced Green Fluorescent Protein expression is observed in the cre-expressing tissues | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | These mice contain an Enhanced Green Fluorescent Protein (EGFP) gene inserted into the Gt(ROSA)26Sor locus. Expression of the EGFP gene is blocked by a loxP-flanked STOP fragment placed between the EGFP sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by EGFP expression in Cre-expressing tissues. [MGI Ref ID J:66742] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm2Sho alt1, Separated PCR
Gt(ROSA)26Sortm2Sho, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Mao X; Fujiwara Y; Chapdelaine A; Yang H; Orkin SH. 2001. Activation of EGFP expression by cre-mediated excision in a new ROSA26 reporter mouse strain Blood 97(1):324-6. [PubMed: 11133778] [MGI Ref ID J:66742]
Additional References
Ohyama T; Groves AK. 2004. Generation of Pax2-Cre mice by modification of a Pax2 bacterial artificial chromosome. Genesis 38(4):195-9. [PubMed: 15083520] [MGI Ref ID J:89784]
Gt(ROSA)26Sortm2Sho relatedAlva JA; Zovein AC; Monvoisin A; Murphy T; Salazar A; Harvey NL; Carmeliet P; Iruela-Arispe ML. 2006. VE-Cadherin-Cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells. Dev Dyn 235(3):759-67. [PubMed: 16450386] [MGI Ref ID J:106155]
Arenkiel BR; Gaufo GO; Capecchi MR. 2003. Hoxb1 neural crest preferentially form glia of the PNS. Dev Dyn 227(3):379-86. [PubMed: 12815623] [MGI Ref ID J:84448]
Balthasar N; Dalgaard LT; Lee CE; Yu J; Funahashi H; Williams T; Ferreira M; Tang V; McGovern RA; Kenny CD; Christiansen LM; Edelstein E; Choi B; Boss O; Aschkenasi C; Zhang CY; Mountjoy K; Kishi T; Elmquist JK; Lowell BB. 2005. Divergence of melanocortin pathways in the control of food intake and energy expenditure. Cell 123(3):493-505. [PubMed: 16269339] [MGI Ref ID J:115192]
Bogunovic M; Ginhoux F; Helft J; Shang L; Hashimoto D; Greter M; Liu K; Jakubzick C; Ingersoll MA; Leboeuf M; Stanley ER; Nussenzweig M; Lira SA; Randolph GJ; Merad M. 2009. Origin of the lamina propria dendritic cell network. Immunity 31(3):513-25. [PubMed: 19733489] [MGI Ref ID J:152688]
Brzezinski JA 4th; Prasov L; Glaser T. 2012. Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle. Dev Biol 365(2):395-413. [PubMed: 22445509] [MGI Ref ID J:184871]
Campbell AL; Shih HP; Xu J; Gross MK; Kioussi C. 2012. Regulation of motility of myogenic cells in filling limb muscle anlagen by Pitx2. PLoS One 7(4):e35822. [PubMed: 22558231] [MGI Ref ID J:187280]
Carhuatanta KA; Demuro G; Tschop MH; Pfluger PT; Benoit SC; Obici S. 2011. Voluntary exercise improves high-fat diet-induced leptin resistance independent of adiposity. Endocrinology 152(7):2655-64. [PubMed: 21586558] [MGI Ref ID J:174884]
Castrop H; Oppermann M; Weiss Y; Huang Y; Mizel D; Lu H; Germain S; Schweda F; Theilig F; Bachmann S; Briggs J; Kurtz A; Schnermann J. 2006. Reporter gene recombination in juxtaglomerular granular and collecting duct cells by human renin promoter-Cre recombinase transgene. Physiol Genomics 25(2):277-85. [PubMed: 16418317] [MGI Ref ID J:144009]
Charles MA; Mortensen AH; Potok MA; Camper SA. 2008. Pitx2 deletion in pituitary gonadotropes is compatible with gonadal development, puberty, and fertility. Genesis 46(10):507-14. [PubMed: 18802953] [MGI Ref ID J:142726]
Chee MJ; Price CJ; Statnick MA; Colmers WF. 2011. Nociceptin/orphanin FQ suppresses the excitability of neurons in the ventromedial nucleus of the hypothalamus. J Physiol 589(Pt 13):3103-14. [PubMed: 21502286] [MGI Ref ID J:189408]
Dessaud E; Ribes V; Balaskas N; Yang LL; Pierani A; Kicheva A; Novitch BG; Briscoe J; Sasai N. 2010. Dynamic assignment and maintenance of positional identity in the ventral neural tube by the morphogen sonic hedgehog. PLoS Biol 8(6):e1000382. [PubMed: 20532235] [MGI Ref ID J:161598]
Duggan CD; Demaria S; Baudhuin A; Stafford D; Ngai J. 2008. Foxg1 is required for development of the vertebrate olfactory system. J Neurosci 28(20):5229-39. [PubMed: 18480279] [MGI Ref ID J:136319]
Eberl G; Littman DR. 2004. Thymic origin of intestinal alphabeta T Cells Revealed by Fate Mapping of RORgammat+ Cells. Science 305(5681):248-51. [PubMed: 15247480] [MGI Ref ID J:91566]
Fogarty M; Grist M; Gelman D; Marin O; Pachnis V; Kessaris N. 2007. Spatial genetic patterning of the embryonic neuroepithelium generates GABAergic interneuron diversity in the adult cortex. J Neurosci 27(41):10935-46. [PubMed: 17928435] [MGI Ref ID J:125700]
Fogarty M; Richardson WD; Kessaris N. 2005. A subset of oligodendrocytes generated from radial glia in the dorsal spinal cord. Development 132(8):1951-9. [PubMed: 15790969] [MGI Ref ID J:101712]
Fogarty MH. 2006. Fate-mapping the mouse neural tube by Cre-loxP transgenesis Doctoral Thesis, University of London :. [MGI Ref ID J:186011]
Frazao R; Cravo RM; Donato J Jr; Ratra DV; Clegg DJ; Elmquist JK; Zigman JM; Williams KW; Elias CF. 2013. Shift in Kiss1 cell activity requires estrogen receptor alpha. J Neurosci 33(7):2807-20. [PubMed: 23407940] [MGI Ref ID J:194265]
Garfield AS; Patterson C; Skora S; Gribble FM; Reimann F; Evans ML; Myers MG Jr; Heisler LK. 2012. Neurochemical characterization of body weight-regulating leptin receptor neurons in the nucleus of the solitary tract. Endocrinology 153(10):4600-7. [PubMed: 22869346] [MGI Ref ID J:191291]
Ghiaur G; Ferkowicz MJ; Milsom MD; Bailey J; Witte D; Cancelas JA; Yoder MC; Williams DA. 2008. Rac1 is essential for intraembryonic hematopoiesis and for the initial seeding of fetal liver with definitive hematopoietic progenitor cells. Blood 111(7):3313-21. [PubMed: 18083846] [MGI Ref ID J:133470]
Gong L; Yao F; Hockman K; Heng HH; Morton GJ; Takeda K; Akira S; Low MJ; Rubinstein M; MacKenzie RG. 2008. Signal transducer and activator of transcription-3 is required in hypothalamic agouti-related protein/neuropeptide Y neurons for normal energy homeostasis. Endocrinology 149(7):3346-54. [PubMed: 18403487] [MGI Ref ID J:145370]
High FA; Zhang M; Proweller A; Tu L; Parmacek MS; Pear WS; Epstein JA. 2007. An essential role for Notch in neural crest during cardiovascular development and smooth muscle differentiation. J Clin Invest 117(2):353-363. [PubMed: 17273555] [MGI Ref ID J:118039]
Hill JW; Williams KW; Ye C; Luo J; Balthasar N; Coppari R; Cowley MA; Cantley LC; Lowell BB; Elmquist JK. 2008. Acute effects of leptin require PI3K signaling in hypothalamic proopiomelanocortin neurons in mice. J Clin Invest 118(5):1796-805. [PubMed: 18382766] [MGI Ref ID J:136147]
Hlady RA; Novakova S; Opavska J; Klinkebiel D; Peters SL; Bies J; Hannah J; Iqbal J; Anderson KM; Siebler HM; Smith LM; Greiner TC; Bastola D; Joshi S; Lockridge O; Simpson MA; Felsher DW; Wagner KU; Chan WC; Christman JK; Opavsky R. 2012. Loss of Dnmt3b function upregulates the tumor modifier Ment and accelerates mouse lymphomagenesis. J Clin Invest 122(1):163-77. [PubMed: 22133874] [MGI Ref ID J:184415]
Jakubzick C; Bogunovic M; Bonito AJ; Kuan EL; Merad M; Randolph GJ. 2008. Lymph-migrating, tissue-derived dendritic cells are minor constituents within steady-state lymph nodes. J Exp Med 205(12):2839-50. [PubMed: 18981237] [MGI Ref ID J:143220]
Jeon D; Kim S; Chetana M; Jo D; Ruley HE; Lin SY; Rabah D; Kinet JP; Shin HS. 2010. Observational fear learning involves affective pain system and Cav1.2 Ca2+ channels in ACC. Nat Neurosci 13(4):482-8. [PubMed: 20190743] [MGI Ref ID J:159698]
Jeselsohn R; Brown NE; Arendt L; Klebba I; Hu MG; Kuperwasser C; Hinds PW. 2010. Cyclin D1 Kinase Activity Is Required for the Self-Renewal of Mammary Stem and Progenitor Cells that Are Targets of MMTV-ErbB2 Tumorigenesis. Cancer Cell 17(1):65-76. [PubMed: 20129248] [MGI Ref ID J:156929]
Johnson JA; Calo S; Nair L; IglayReger HB; Greenwald-Yarnell M; Skorupski J; Myers MG Jr; Bodary PF. 2012. Testosterone interacts with the feedback mechanisms engaged by Tyr985 of the leptin receptor and diet-induced obesity. J Steroid Biochem Mol Biol 132(3-5):212-9. [PubMed: 22750459] [MGI Ref ID J:190163]
Kaplan DH; Li MO; Jenison MC; Shlomchik WD; Flavell RA; Shlomchik MJ. 2007. Autocrine/paracrine TGF{beta}1 is required for the development of epidermal Langerhans cells. J Exp Med 204(11):2545-52. [PubMed: 17938236] [MGI Ref ID J:126147]
Kessaris N; Fogarty M; Iannarelli P; Grist M; Wegner M; Richardson WD. 2006. Competing waves of oligodendrocytes in the forebrain and postnatal elimination of an embryonic lineage. Nat Neurosci 9(2):173-9. [PubMed: 16388308] [MGI Ref ID J:105334]
Kim HJ; Kobayashi M; Sasaki T; Kikuchi O; Amano K; Kitazumi T; Lee YS; Yokota-Hashimoto H; Susanti VY; Kitamura YI; Nakae J; Kitamura T. 2012. Overexpression of FoxO1 in the hypothalamus and pancreas causes obesity and glucose intolerance. Endocrinology 153(2):659-71. [PubMed: 22186407] [MGI Ref ID J:181720]
Knauf JA; Sartor MA; Medvedovic M; Lundsmith E; Ryder M; Salzano M; Nikiforov YE; Giordano TJ; Ghossein RA; Fagin JA. 2011. Progression of BRAF-induced thyroid cancer is associated with epithelial-mesenchymal transition requiring concomitant MAP kinase and TGFbeta signaling. Oncogene 30(28):3153-62. [PubMed: 21383698] [MGI Ref ID J:174636]
Lam DD; Leinninger GM; Louis GW; Garfield AS; Marston OJ; Leshan RL; Scheller EL; Christensen L; Donato J Jr; Xia J; Evans ML; Elias C; Dalley JW; Burdakov DI; Myers MG Jr; Heisler LK. 2011. Leptin does not directly affect CNS serotonin neurons to influence appetite. Cell Metab 13(5):584-91. [PubMed: 21531340] [MGI Ref ID J:175812]
Leinninger GM; Jo YH; Leshan RL; Louis GW; Yang H; Barrera JG; Wilson H; Opland DM; Faouzi MA; Gong Y; Jones JC; Rhodes CJ; Chua S Jr; Diano S; Horvath TL; Seeley RJ; Becker JB; Munzberg H; Myers MG Jr. 2009. Leptin acts via leptin receptor-expressing lateral hypothalamic neurons to modulate the mesolimbic dopamine system and suppress feeding. Cell Metab 10(2):89-98. [PubMed: 19656487] [MGI Ref ID J:151971]
Leinninger GM; Opland DM; Jo YH; Faouzi M; Christensen L; Cappellucci LA; Rhodes CJ; Gnegy ME; Becker JB; Pothos EN; Seasholtz AF; Thompson RC; Myers MG Jr. 2011. Leptin Action via Neurotensin Neurons Controls Orexin, the Mesolimbic Dopamine System and Energy Balance. Cell Metab 14(3):313-23. [PubMed: 21907138] [MGI Ref ID J:176657]
Leshan RL; Louis GW; Jo YH; Rhodes CJ; Munzberg H; Myers MG Jr. 2009. Direct innervation of GnRH neurons by metabolic- and sexual odorant-sensing leptin receptor neurons in the hypothalamic ventral premammillary nucleus. J Neurosci 29(10):3138-47. [PubMed: 19279251] [MGI Ref ID J:147070]
Leshan RL; Opland DM; Louis GW; Leinninger GM; Patterson CM; Rhodes CJ; Munzberg H; Myers MG Jr. 2010. Ventral tegmental area leptin receptor neurons specifically project to and regulate cocaine- and amphetamine-regulated transcript neurons of the extended central amygdala. J Neurosci 30(16):5713-23. [PubMed: 20410123] [MGI Ref ID J:159836]
Liao CP; Zhong C; Saribekyan G; Bading J; Park R; Conti PS; Moats R; Berns A; Shi W; Zhou Z; Nikitin AY; Roy-Burman P. 2007. Mouse models of prostate adenocarcinoma with the capacity to monitor spontaneous carcinogenesis by bioluminescence or fluorescence. Cancer Res 67(15):7525-33. [PubMed: 17671224] [MGI Ref ID J:123911]
Lin HV; Ren H; Samuel VT; Lee HY; Lu TY; Shulman GI; Accili D. 2011. Diabetes in mice with selective impairment of insulin action in glut4-expressing tissues. Diabetes 60(3):700-9. [PubMed: 21266328] [MGI Ref ID J:169404]
Liu Z; Dearman JA; Cox BC; Walters BJ; Zhang L; Ayrault O; Zindy F; Gan L; Roussel MF; Zuo J. 2012. Age-dependent in vivo conversion of mouse cochlear pillar and deiters' cells to immature hair cells by Atoh1 ectopic expression. J Neurosci 32(19):6600-10. [PubMed: 22573682] [MGI Ref ID J:184850]
Louis GW; Leinninger GM; Rhodes CJ; Myers MG Jr. 2010. Direct innervation and modulation of orexin neurons by lateral hypothalamic LepRb neurons. J Neurosci 30(34):11278-87. [PubMed: 20739548] [MGI Ref ID J:163528]
Lu F; Kar D; Gruenig N; Zhang ZW; Cousins N; Rodgers HM; Swindell EC; Jamrich M; Schuurmans C; Mathers PH; Kurrasch DM. 2013. Rax is a selector gene for mediobasal hypothalamic cell types. J Neurosci 33(1):259-72. [PubMed: 23283339] [MGI Ref ID J:193919]
Lu J; Sun Y; Nombela-Arrieta C; Du KP; Park SY; Chai L; Walkley C; Luo HR; Silberstein LE. 2012. Fak depletion in both hematopoietic and nonhematopoietic niche cells leads to hematopoietic stem cell expansion. Exp Hematol 40(4):307-17.e3. [PubMed: 22155722] [MGI Ref ID J:191529]
Luche H; Weber O; Nageswara Rao T; Blum C; Fehling HJ. 2007. Faithful activation of an extra-bright red fluorescent protein in 'knock-in' Cre-reporter mice ideally suited for lineage tracing studies. Eur J Immunol 37(1):43-53. [PubMed: 17171761] [MGI Ref ID J:117041]
Matsuki T; Nomiyama M; Takahira H; Hirashima N; Kunita S; Takahashi S; Yagami K; Kilduff TS; Bettler B; Yanagisawa M; Sakurai T. 2009. Selective loss of GABA(B) receptors in orexin-producing neurons results in disrupted sleep/wakefulness architecture. Proc Natl Acad Sci U S A 106(11):4459-64. [PubMed: 19246384] [MGI Ref ID J:146778]
Mayne CG; Spanier JA; Relland LM; Williams CB; Hayes CE. 2011. 1,25-Dihydroxyvitamin D3 acts directly on the T lymphocyte vitamin D receptor to inhibit experimental autoimmune encephalomyelitis. Eur J Immunol 41(3):822-32. [PubMed: 21287548] [MGI Ref ID J:175424]
Morgans CW; Zhang J; Jeffrey BG; Nelson SM; Burke NS; Duvoisin RM; Brown RL. 2009. TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells. Proc Natl Acad Sci U S A 106(45):19174-8. [PubMed: 19861548] [MGI Ref ID J:154776]
Mukhopadhyay A; McGuire T; Peng CY; Kessler JA. 2009. Differential effects of BMP signaling on parvalbumin and somatostatin interneuron differentiation. Development 136(15):2633-42. [PubMed: 19592576] [MGI Ref ID J:158140]
Muller AJ; Filipe-Santos O; Eberl G; Aebischer T; Spath GF; Bousso P. 2012. CD4+ T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation. Immunity 37(1):147-57. [PubMed: 22727490] [MGI Ref ID J:187413]
Murray SA; Oram KF; Gridley T. 2007. Multiple functions of Snail family genes during palate development in mice. Development 134(9):1789-97. [PubMed: 17376812] [MGI Ref ID J:121243]
Nern C; Wolff I; Macas J; von Randow J; Scharenberg C; Priller J; Momma S. 2009. Fusion of hematopoietic cells with Purkinje neurons does not lead to stable heterokaryon formation under noninvasive conditions. J Neurosci 29(12):3799-807. [PubMed: 19321776] [MGI Ref ID J:158171]
Ohinata Y; Payer B; O'Carroll D; Ancelin K; Ono Y; Sano M; Barton SC; Obukhanych T; Nussenzweig M; Tarakhovsky A; Saitou M; Surani MA. 2005. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436(7048):207-13. [PubMed: 15937476] [MGI Ref ID J:99994]
Opavsky R; Wang SH; Trikha P; Raval A; Huang Y; Wu YZ; Rodriguez B; Keller B; Liyanarachchi S; Wei G; Davuluri RV; Weinstein M; Felsher D; Ostrowski M; Leone G; Plass C. 2007. CpG island methylation in a mouse model of lymphoma is driven by the genetic configuration of tumor cells. PLoS Genet 3(9):1757-69. [PubMed: 17907813] [MGI Ref ID J:130029]
Padilla SL; Carmody JS; Zeltser LM. 2010. Pomc-expressing progenitors give rise to antagonistic neuronal populations in hypothalamic feeding circuits. Nat Med 16(4):403-5. [PubMed: 20348924] [MGI Ref ID J:159306]
Park SY; Wolfram P; Canty K; Harley B; Nombela-Arrieta C; Pivarnik G; Manis J; Beggs HE; Silberstein LE. 2013. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments. J Immunol 190(3):1094-102. [PubMed: 23264658] [MGI Ref ID J:192606]
Peng CY; Mukhopadhyay A; Jarrett JC; Yoshikawa K; Kessler JA. 2012. BMP receptor 1A regulates development of hypothalamic circuits critical for feeding behavior. J Neurosci 32(48):17211-24. [PubMed: 23197713] [MGI Ref ID J:193055]
Pepper M; Pagan AJ; Igyarto BZ; Taylor JJ; Jenkins MK. 2011. Opposing signals from the bcl6 transcription factor and the interleukin-2 receptor generate T helper 1 central and effector memory cells. Immunity 35(4):583-95. [PubMed: 22018468] [MGI Ref ID J:177635]
Plum L; Lin HV; Dutia R; Tanaka J; Aizawa KS; Matsumoto M; Kim AJ; Cawley NX; Paik JH; Loh YP; DePinho RA; Wardlaw SL; Accili D. 2009. The obesity susceptibility gene Cpe links FoxO1 signaling in hypothalamic pro-opiomelanocortin neurons with regulation of food intake. Nat Med 15(10):1195-201. [PubMed: 19767734] [MGI Ref ID J:154300]
Provenzano PP; Cuevas C; Chang AE; Goel VK; Von Hoff DD; Hingorani SR. 2012. Enzymatic targeting of the stroma ablates physical barriers to treatment of pancreatic ductal adenocarcinoma. Cancer Cell 21(3):418-29. [PubMed: 22439937] [MGI Ref ID J:189345]
Rossi J; Balthasar N; Olson D; Scott M; Berglund E; Lee CE; Choi MJ; Lauzon D; Lowell BB; Elmquist JK. 2011. Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab 13(2):195-204. [PubMed: 21284986] [MGI Ref ID J:169562]
Rubin AN; Alfonsi F; Humphreys MP; Choi CK; Rocha SF; Kessaris N. 2010. The germinal zones of the basal ganglia but not the septum generate GABAergic interneurons for the cortex. J Neurosci 30(36):12050-62. [PubMed: 20826668] [MGI Ref ID J:164291]
Schaefer A; O'Carroll D; Tan CL; Hillman D; Sugimori M; Llinas R; Greengard P. 2007. Cerebellar neurodegeneration in the absence of microRNAs. J Exp Med 204(7):1553-8. [PubMed: 17606634] [MGI Ref ID J:125878]
Stefater MA; MacLennan AJ; Lee N; Patterson CM; Haller A; Sorrell J; Myers M; Woods SC; Seeley RJ. 2012. The anorectic effect of CNTF does not require action in leptin-responsive neurons. Endocrinology 153(6):2647-54. [PubMed: 22518062] [MGI Ref ID J:188659]
Su H; Marcheva B; Meng S; Liang FA; Kohsaka A; Kobayashi Y; Xu AW; Bass J; Wang X. 2010. Gamma-protocadherins regulate the functional integrity of hypothalamic feeding circuitry in mice. Dev Biol 339(1):38-50. [PubMed: 20025866] [MGI Ref ID J:157958]
Sugrue ML; Vella KR; Morales C; Lopez ME; Hollenberg AN. 2010. The thyrotropin-releasing hormone gene is regulated by thyroid hormone at the level of transcription in vivo. Endocrinology 151(2):793-801. [PubMed: 20032051] [MGI Ref ID J:162058]
Talchai C; Xuan S; Kitamura T; DePinho RA; Accili D. 2012. Generation of functional insulin-producing cells in the gut by Foxo1 ablation. Nat Genet 44(4):406-12, S1. [PubMed: 22406641] [MGI Ref ID J:183890]
Tavazoie SF; Alvarez VA; Ridenour DA; Kwiatkowski DJ; Sabatini BL. 2005. Regulation of neuronal morphology and function by the tumor suppressors Tsc1 and Tsc2. Nat Neurosci 8(12):1727-34. [PubMed: 16286931] [MGI Ref ID J:103933]
Vallespinos M; Fernandez D; Rodriguez L; Alvaro-Blanco J; Baena E; Ortiz M; Dukovska D; Martinez D; Rojas A; Campanero MR; Moreno de Alboran I. 2011. B Lymphocyte commitment program is driven by the proto-oncogene c-Myc. J Immunol 186(12):6726-36. [PubMed: 21572027] [MGI Ref ID J:175485]
Vidali L; Chen F; Cicchetti G; Ohta Y; Kwiatkowski DJ. 2006. Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor. Mol Biol Cell 17(5):2377-90. [PubMed: 16525021] [MGI Ref ID J:112526]
Villanueva EC; Munzberg H; Cota D; Leshan RL; Kopp K; Ishida-Takahashi R; Jones JC; Fingar DC; Seeley RJ; Myers MG Jr. 2009. Complex regulation of mammalian target of rapamycin complex 1 in the basomedial hypothalamus by leptin and nutritional status. Endocrinology 150(10):4541-51. [PubMed: 19628573] [MGI Ref ID J:157328]
Xu L; Scheenen WJ; Leshan RL; Patterson CM; Elias CF; Bouwhuis S; Roubos EW; Myers MG Jr; Kozicz T. 2011. Leptin signaling modulates the activity of urocortin 1 neurons in the mouse nonpreganglionic Edinger-Westphal nucleus. Endocrinology 152(3):979-88. [PubMed: 21209012] [MGI Ref ID J:173887]
Yang ZJ; Ellis T; Markant SL; Read TA; Kessler JD; Bourboulas M; Schuller U; Machold R; Fishell G; Rowitch DH; Wainwright BJ; Wechsler-Reya RJ. 2008. Medulloblastoma can be initiated by deletion of Patched in lineage-restricted progenitors or stem cells. Cancer Cell 14(2):135-45. [PubMed: 18691548] [MGI Ref ID J:139573]
Yi L; Domyan ET; Lewandoski M; Sun X. 2008. Fibroblast growth factor 9 signaling inhibits airway smooth muscle differentiation in mouse lung. Dev Dyn 238(1):123-137. [PubMed: 19097117] [MGI Ref ID J:142646]
Young KM; Mitsumori T; Pringle N; Grist M; Kessaris N; Richardson WD. 2010. An Fgfr3-iCreER(T2) transgenic mouse line for studies of neural stem cells and astrocytes. Glia 58(8):943-53. [PubMed: 20155815] [MGI Ref ID J:159164]
Yuan TL; Choi HS; Matsui A; Benes C; Lifshits E; Luo J; Frangioni JV; Cantley LC. 2008. Class 1A PI3K regulates vessel integrity during development and tumorigenesis. Proc Natl Acad Sci U S A 105(28):9739-44. [PubMed: 18621722] [MGI Ref ID J:137878]
Zhang J; Zhong W; Cui T; Yang M; Hu X; Xu K; Xie C; Xue C; Gibbons GH; Liu C; Li L; Chen YE. 2006. Generation of an adult smooth muscle cell-targeted Cre recombinase mouse model. Arterioscler Thromb Vasc Biol 26(3):e23-4. [PubMed: 16484601] [MGI Ref ID J:119865]
Zhou W; Zhang Y; Li Y; Wei YS; Liu G; Liu DP; Pleasure SJ; Xie W; Zhao C. 2010. A transgenic Cre mouse line for the study of cortical and hippocampal development. Genesis 48(5):343-50. [PubMed: 20143346] [MGI Ref ID J:160192]
Zhou X; Xue HH. 2012. Cutting edge: generation of memory precursors and functional memory CD8+ T cells depends on T cell factor-1 and lymphoid enhancer-binding factor-1. J Immunol 189(6):2722-6. [PubMed: 22875805] [MGI Ref ID J:189838]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB27
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129 background and can be maintained as a hemizygote or homozygote. Coat color expected from breeding: Black Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Gt(ROSA)26Sortm2Sho
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Gt(ROSA)26Sortm2Sho x Homozygous for Gt(ROSA)26Sortm2Sho Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Gt(ROSA)26Sortm2Sho
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Gt(ROSA)26Sortm2Sho x Homozygous for Gt(ROSA)26Sortm2Sho Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.