Strain Name: |
FVB-Tg(AZIP/F)1Vsn/J |
|---|---|
Stock Number: |
004100 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Fabp4; A-ZIP/F; Tg(AZIP/F)1Vsn; |
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Charles Vinson, NCI, NIH Strain Description
Mice expressing the A-ZIP/F gene under the control of 7.6kB of the adipocyte specific Fabp4 promoter have virtually no white adipose tissue and a reduced amount of brown adipose tissue. Brown adipose is found in lesser amounts as the animal ages. These mutant mice are very sensitive to cold temperature. Hemizygotes suffer from poor fecundity; the donating investigator indicates that not all males breed and pregnant females lactate inadequately. At birth, mice appear normal, losing weight in the first post-natal week but reach or exceed wildtype weight by 8-11 weeks. Adult mice display polyphagia and polydypsia, eating and drinking several times more than wildtype mice. Urine output is greatly increased. Transgenic mice have a characteristic rough-appearing coat and increased abdominal girth attributed to enlarged viscera. Most notably affected is the liver. Histological examination of liver tissue reveals numerous lipid droplets. Pancreatic islets are hypertrophic and hyperplastic with large increases in beta cell numbers observed. Adult mice exhibit elevated levels of glucose, insulin, free fatty acids and triglycerides. Increased insulin levels are observed as early as 5 weeks of age. Circulating levels of free, active leptin are greatly diminished. This strain represents a model useful for studying lipoatrophicdiabetes and the contribution of fat tissue to metabolic equilibrium.Strain Development
A transgenic construct containing the A-ZIP/F gene under the direction of a fatty acid binding protein 4, adipocyte promoter/enhancer was injected into male pronuclei of FVB/N mice. Founder animals were obtained and mated with FVB/N mice.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tg(AZIP/F)1Vsn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Charles Vincent | ||
| Common Name(s) | A-ZIP/F-1; | ||
| Mutation Made By | Charles Vinson, NCI, NIH | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | A-ZIP/F, flag epitope/leucine zipper, artificial | ||
| Promoter | Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory | ||
| General Note | The A-ZIP/F-1 phenotype suggests a mouse model for the human disease lipoatrophic diabetes (Seip-Berardinelli syndrome). | ||
| Molecular Note | A 7.6 kb segment of the Fabp4 enhancer/promoter was used to drive expression of an artificially constructed cDNA encoding a dominant-negative protein to inhibit the DNA binding of both Cebpa and Jun family members. The protein is 104 amino acids long, with an amino-terminal 9 amino acid Flag epitope, a 13 amino acid linker, a 21 amino acid designed acidic amphipathic helix and a 61 amino acid designed leucine zipper (ZIP/F). The transgene also had an SV40 intron and a PolyA signal. [MGI Ref ID J:50807] | ||
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Tg(AZIP/F)1Vsn
| Breeding & Husbandry | These mice originated on an FVB/N background and have been maintained as hemizygotes by breeding to FVB mice. Hemizygotes suffer from poor fecundity; the donating investigator indicates that not all males breed and pregnant females lactate inadequately. Use of nestlets recommended due to cold sensitivity and extra absorbent bedding due to excessive urination. |
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| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying other alleles of Fabp4
005069 B6.Cg-Tg(Fabp4-cre)1Rev/J 003393 B6;SJL-Tg(aP2-SREBF1c)9884Reh/J View Strains carrying other alleles of Fabp4 (2 strains)
Diabetes and Obesity Research
Hyperglycemia
Hyperinsulinemia
Type 2 Diabetes (NIDDM)
Metabolism Research
Lipid Metabolism
Selected Reference(s)
Additional ReferencesMoitra J; Mason MM; Olive M; Krylov D; Gavrilova O; Marcus-Samuels B; Feigenbaum L; Lee E; Aoyama T; Eckhaus M; Reitman ML; Vinson C. 1998. Life without white fat: a transgenic mouse. Genes Dev 12(20):3168-81. [PubMed: 9784492] [MGI Ref ID J:50807]
| Strain Name: | FVB-Tg(AZIP/F)1Vsn/J |
| Stock Number: | 004100 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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