Strain Name:

B6;129P-Ccr4tm1Pwr/J

Stock Number:

004101

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129P-Cmkbr4tm1Pwr    (Changed: 15-DEC-04 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Christine Power,   Serono Pharmaceutical Research Institute

Description
Mice that are homozygous null for the Ccr4 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Ccr4 gene product (mRNA or protein) is detected. Although some normal responses to bacterial lipopolysaccharide (LPS) administration are observed (shivering and lethargy), these mice are resistant to LPS-induced lethality. Significantly lower levels of serum tumor necrosis factor alpha and interleukin 1 beta are observed in response to LPS injection. Splenocytes and thymocytes show no chemotactic response to Ccr4 ligands and diminished macrophage levels are observed in thioglycollate-elicited peritoneal cells.

Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the entire Ccr4 coding region. The construct was electroporated into 129P2/OlaHsd-derived HM-1 embryonic stem cells and selected cells were injected into C57BL/6J blastocysts.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ccr4tm1Pwr/Ccr4tm1Pwr

        involves: 129P2/OlaHsd * C57BL/6
  • immune system phenotype
  • *normal* immune system phenotype
    • in vitro, homozygotes display normal Th2 and Th1 cell differentiation relative to wild-type mice   (MGI Ref ID J:70088)
    • in an OVA-induced model of airway inflammation, homozygotes display normal Th2-associated bronchial hyperreactivity in response to inhaled methacholine after OVA priming and intranasal challenge with saline, with no differences in OVA-induced eosinophilia in BALF and lung tissue, in total cell count or individual leukocyte populations in BALF, or in OVA-specific Ig titers relative to wild-type mice   (MGI Ref ID J:70088)
    • abnormal cytokine secretion   (MGI Ref ID J:120221)
      • abnormal chemokine secretion
        • at 3 hrs after injection of a high dose of LPS (60 mg/kg), homozygotes show a significant decrease in serum MIP-alpha levels relative to wild-type mice, suggesting a possible macrophage defect   (MGI Ref ID J:70088)
      • decreased interleukin-1 beta secretion
        • at 3 hrs after injection of a high dose of LPS (60 mg/kg), homozygotes show a 6-fold decrease in serum IL-1beta levels relative to wild-type mice   (MGI Ref ID J:70088)
        • in contrast, IL-6 production remains unchanged relative to wild-type mice   (MGI Ref ID J:70088)
      • decreased interleukin-10 secretion
        • 30 days after A. fumigatus infection   (MGI Ref ID J:120221)
      • decreased interleukin-13 secretion
        • 30 days after A. fumigatus infection   (MGI Ref ID J:120221)
      • decreased interleukin-4 secretion
        • 30 days after A. fumigatus infection   (MGI Ref ID J:120221)
      • decreased interleukin-5 secretion
        • 30 days after A. fumigatus infection   (MGI Ref ID J:120221)
      • decreased tumor necrosis factor secretion
        • in response to a high LPS dose (60 mg/kg), homozygotes fail to induce significant levels of serum TNF, whereas wild-type mice show a sharp increase in serum TNF levels at 1.5 hrs which return to baseline by 4 hrs after LPS treatment   (MGI Ref ID J:70088)
        • in vitro, peritoneal lavage cells from naive homozygotes produce significantly lower TNF levels when cultured for 18 hrs in the presence of 1 g/ml LPS relative to cells from naive wild-type mice (107 11 versus 201 18 pg/ml, respectively)   (MGI Ref ID J:70088)
      • increased interferon-gamma secretion
        • 3 days after A. fumigatus infection   (MGI Ref ID J:120221)
      • increased interleukin-10 secretion
        • 3 days after A. fumigatus infection   (MGI Ref ID J:120221)
      • increased interleukin-4 secretion
        • 3 days after A. fumigatus infection   (MGI Ref ID J:120221)
      • increased interleukin-5 secretion
        • 3 days after A. fumigatus infection   (MGI Ref ID J:120221)
      • increased tumor necrosis factor secretion
        • after A. fumigatus infection   (MGI Ref ID J:120221)
    • abnormal splenocyte physiology
      • unexpectedly, mutant splenocytes show no chemotactic response to human or mouse chemokine (C-C motif) ligand 3 (also known as macrophage inflammatory protein MIP-1alpha)   (MGI Ref ID J:70088)
      • in contrast, the response of mutant splenocytes to human RANTES is similar to that observed in wild-type mice   (MGI Ref ID J:70088)
    • decreased IgE level
      • 30 days after A. fumigatus infection   (MGI Ref ID J:120221)
    • decreased eosinophil cell number
      • after A. fumigatus infection in the bronchoalveolar lavage   (MGI Ref ID J:120221)
    • decreased lymphocyte cell number
      • at 24 hrs after high dose LPS treatment, homozygotes show a reduction in the number of lymphocytes in the peritoneal lavage relative to wild-type mice   (MGI Ref ID J:70088)
    • decreased macrophage cell number
      • at 24 hrs after high dose LPS treatment, homozygotes display significantly fewer macrophages in the peritoneal lavage, with reduced numbers of CD11b+ and CD14+ cells and an absence or severely reduced number of F4/80-expressing cells relative to wild-type mice   (MGI Ref ID J:70088)
    • decreased susceptibility to endotoxin shock
      • in response to high doses of LPS (60-120 mg/kg), homozygotes show no differences in initial platelet mobilization but display milder signs of shivering and lethargy than wild-type during the first few hours after LPS injection, with platelet counts returning to normal at day 5 and 93% of homozygotes still alive on day 6, whereas all wild-type die between 2 and 4 days after treatment   (MGI Ref ID J:70088)
      • in response to a low dose of LPS (1 g) plus 8 mg of D-gal, 9 of 12 homozygotes survive whereas the majority of wild-type (11 of 12) die; following treatment with 4 g of LPS plus D-gal, all homozygotes tested die with a 6-hr delay relative to wild-type mice   (MGI Ref ID J:70088)
    • decreased susceptibility to fungal infection
      • 7 days after Aspergillus fumigatus infection, mice clear fungal spores from the lungs; increased neutrophil, eosinophil, and macrophage numbers in the bronchoalveolar lavage (BAL); increased early airway resistance; increased early levels of TNFalpha, IFN-gamma, IL4, IL5, and IL10 in the BAL; and increased early IgE and IgG1 levels compared with wild-type mice   (MGI Ref ID J:120221)
      • 3 days after A. fumigatus infection airway resistance is increased compared to in wild-type mice   (MGI Ref ID J:120221)
      • 30 days after A. fumigatus infection airway resistance is decreased compared to in wild-type mice   (MGI Ref ID J:120221)
    • increased IgE level
      • 3 and 7 days after A. fumigatus infection   (MGI Ref ID J:120221)
    • increased IgG1 level
      • 3 days after A. fumigatus infection   (MGI Ref ID J:120221)
    • increased macrophage cell number
      • after A. fumigatus infection in the bronchoalveolar lavage   (MGI Ref ID J:120221)
    • increased neutrophil cell number
      • after A. fumigatus infection in the bronchoalveolar lavage   (MGI Ref ID J:120221)
  • respiratory system phenotype
  • decreased airway resistance
    • 30 days after A. fumigatus infection   (MGI Ref ID J:120221)
  • increased airway resistance
    • 3 days after A. fumigatus infection   (MGI Ref ID J:120221)
  • hematopoietic system phenotype
  • abnormal splenocyte physiology
    • unexpectedly, mutant splenocytes show no chemotactic response to human or mouse chemokine (C-C motif) ligand 3 (also known as macrophage inflammatory protein MIP-1alpha)   (MGI Ref ID J:70088)
    • in contrast, the response of mutant splenocytes to human RANTES is similar to that observed in wild-type mice   (MGI Ref ID J:70088)
  • decreased IgE level
    • 30 days after A. fumigatus infection   (MGI Ref ID J:120221)
  • decreased eosinophil cell number
    • after A. fumigatus infection in the bronchoalveolar lavage   (MGI Ref ID J:120221)
  • decreased lymphocyte cell number
    • at 24 hrs after high dose LPS treatment, homozygotes show a reduction in the number of lymphocytes in the peritoneal lavage relative to wild-type mice   (MGI Ref ID J:70088)
  • decreased macrophage cell number
    • at 24 hrs after high dose LPS treatment, homozygotes display significantly fewer macrophages in the peritoneal lavage, with reduced numbers of CD11b+ and CD14+ cells and an absence or severely reduced number of F4/80-expressing cells relative to wild-type mice   (MGI Ref ID J:70088)
  • increased IgE level
    • 3 and 7 days after A. fumigatus infection   (MGI Ref ID J:120221)
  • increased IgG1 level
    • 3 days after A. fumigatus infection   (MGI Ref ID J:120221)
  • increased macrophage cell number
    • after A. fumigatus infection in the bronchoalveolar lavage   (MGI Ref ID J:120221)
  • increased neutrophil cell number
    • after A. fumigatus infection in the bronchoalveolar lavage   (MGI Ref ID J:120221)

Ccr4tm1Pwr/Ccr4tm1Pwr

        B6.129P2-Ccr4tm1Pwr
  • immune system phenotype
  • increased CD8-positive T cell number
    • in cardiac grafts   (MGI Ref ID J:95239)
  • increased NK T cell number
    • in cardiac grafts   (MGI Ref ID J:95239)
  • increased length of allograft survival
    • in cardiac grafts   (MGI Ref ID J:95239)
  • hematopoietic system phenotype
  • increased CD8-positive T cell number
    • in cardiac grafts   (MGI Ref ID J:95239)
  • increased NK T cell number
    • in cardiac grafts   (MGI Ref ID J:95239)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Ccr4tm1Pwr related

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ccr4tm1Pwr
Allele Name targeted mutation 1, Christine Power
Allele Type Targeted (knock-out)
Common Name(s) CCR4-; Ccr4-;
Mutation Made By Christine Power,   Serono Pharmaceutical Research Institute
Strain of Origin129P2/OlaHsd-Hprt
ES Cell Line NameHM-1
ES Cell Line Strain129P2/OlaHsd-Hprt
Gene Symbol and Name Ccr4, chemokine (C-C motif) receptor 4
Chromosome 9
Gene Common Name(s) CC CKR-4; CC-CKR-4; CD194; CKR4; CMKBR4; ChemR13; Cmkbr4; HGCN:14099; K5-5; chemokine (C-C) receptor 4;
Molecular Note A neomycin resistance cassette replaced the entire coding sequence and part of the 5' noncoding region. RT-PCR did not amplify transcript from thymus, spleen, or peritoneal macrophage cells of homozygous mutant mice. [MGI Ref ID J:70088]

Genotyping

Genotyping Information

Genotyping Protocols

Ccr4tm1Pwralternate1,

Separated MCA


Ccr4tm1Pwralternate1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Chvatchko Y; Hoogewerf AJ; Meyer A; Alouani S; Juillard P; Buser R; Conquet F; Proudfoot AE; Wells TN; Power CA. 2000. A key role for CC chemokine receptor 4 in lipopolysaccharide-induced endotoxic shock. J Exp Med 191(10):1755-64. [PubMed: 10811868]  [MGI Ref ID J:70088]

Additional References

Ccr4tm1Pwr related

Baekkevold ES; Wurbel MA; Kivisakk P; Wain CM; Power CA; Haraldsen G; Campbell JJ. 2005. A role for CCR4 in development of mature circulating cutaneous T helper memory cell populations. J Exp Med 201(7):1045-51. [PubMed: 15795234]  [MGI Ref ID J:98003]

Carpenter KJ; Hogaboam CM. 2005. Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis. Infect Immun 73(11):7198-207. [PubMed: 16239514]  [MGI Ref ID J:104233]

Freeman CM; Stolberg VR; Chiu BC; Lukacs NW; Kunkel SL; Chensue SW. 2006. CCR4 participation in Th type 1 (mycobacterial) and Th type 2 (schistosomal) anamnestic pulmonary granulomatous responses. J Immunol 177(6):4149-58. [PubMed: 16951380]  [MGI Ref ID J:138040]

Freyschmidt EJ; Mathias CB; Diaz N; MacArthur DH; Laouar A; Manjunath N; Hofer MD; Wurbel MA; Campbell JJ; Chatila TA; Oettgen HC. 2010. Skin inflammation arising from cutaneous regulatory T cell deficiency leads to impaired viral immune responses. J Immunol 185(2):1295-302. [PubMed: 20548030]  [MGI Ref ID J:161939]

Huser N; Tertilt C; Gerauer K; Maier S; Traeger T; Assfalg V; Reiter R; Heidecke CD; Pfeffer K. 2005. CCR4-deficient mice show prolonged graft survival in a chronic cardiac transplant rejection model. Eur J Immunol 35(1):128-38. [PubMed: 15593118]  [MGI Ref ID J:95239]

Ishii M; Hogaboam CM; Joshi A; Ito T; Fong DJ; Kunkel SL. 2008. CC chemokine receptor 4 modulates Toll-like receptor 9-mediated innate immunity and signaling. Eur J Immunol 38(8):2290-302. [PubMed: 18624303]  [MGI Ref ID J:138562]

Islam SA; Chang DS; Colvin RA; Byrne MH; McCully ML; Moser B; Lira SA; Charo IF; Luster AD. 2011. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat Immunol 12(2):167-77. [PubMed: 21217759]  [MGI Ref ID J:168253]

Jessup HK; Brewer AW; Omori M; Rickel EA; Budelsky AL; Yoon BR; Ziegler SF; Comeau MR. 2008. Intradermal administration of thymic stromal lymphopoietin induces a T cell- and eosinophil-dependent systemic Th2 inflammatory response. J Immunol 181(6):4311-9. [PubMed: 18768889]  [MGI Ref ID J:139079]

Jiang X; Campbell JJ; Kupper TS. 2010. Embryonic trafficking of gammadelta T cells to skin is dependent on E/P selectin ligands and CCR4. Proc Natl Acad Sci U S A 107(16):7443-8. [PubMed: 20368416]  [MGI Ref ID J:159289]

Lehtimaki S; Tillander S; Puustinen A; Matikainen S; Nyman T; Fyhrquist N; Savinko T; Majuri ML; Wolff H; Alenius H; Lauerma A. 2010. Absence of CCR4 Exacerbates Skin Inflammation in an Oxazolone-Induced Contact Hypersensitivity Model. J Invest Dermatol 130(12):2743-51. [PubMed: 20631725]  [MGI Ref ID J:165832]

Mikhak Z; Strassner JP; Luster AD. 2013. Lung dendritic cells imprint T cell lung homing and promote lung immunity through the chemokine receptor CCR4. J Exp Med 210(9):1855-69. [PubMed: 23960189]  [MGI Ref ID J:202485]

Miyajima M; Chase CM; Alessandrini A; Farkash EA; Della Pelle P; Benichou G; Graham JA; Madsen JC; Russell PS; Colvin RB. 2011. Early acceptance of renal allografts in mice is dependent on foxp3(+) cells. Am J Pathol 178(4):1635-45. [PubMed: 21435448]  [MGI Ref ID J:169852]

Nakamura K; White AJ; Parnell SM; Lane PJ; Jenkinson EJ; Jenkinson WE; Anderson G. 2013. Differential requirement for CCR4 in the maintenance but not establishment of the invariant Vgamma5(+) dendritic epidermal T-cell pool. PLoS One 8(9):e74019. [PubMed: 24069263]  [MGI Ref ID J:207537]

Ness TL; Ewing JL; Hogaboam CM; Kunkel SL. 2006. CCR4 is a key modulator of innate immune responses. J Immunol 177(11):7531-9. [PubMed: 17114422]  [MGI Ref ID J:140604]

Onodera T; Jang MH; Guo Z; Yamasaki M; Hirata T; Bai Z; Tsuji NM; Nagakubo D; Yoshie O; Sakaguchi S; Takikawa O; Miyasaka M. 2009. Constitutive expression of IDO by dendritic cells of mesenteric lymph nodes: functional involvement of the CTLA-4/B7 and CCL22/CCR4 interactions. J Immunol 183(9):5608-14. [PubMed: 19843945]  [MGI Ref ID J:156794]

Oyoshi MK; Elkhal A; Scott JE; Wurbel MA; Hornick JL; Campbell JJ; Geha RS. 2011. Epicutaneous challenge of orally immunized mice redirects antigen-specific gut-homing T cells to the skin. J Clin Invest 121(6):2210-20. [PubMed: 21537081]  [MGI Ref ID J:173920]

Poppensieker K; Otte DM; Schurmann B; Limmer A; Dresing P; Drews E; Schumak B; Klotz L; Raasch J; Mildner A; Waisman A; Scheu S; Knolle P; Forster I; Prinz M; Maier W; Zimmer A; Alferink J. 2012. CC chemokine receptor 4 is required for experimental autoimmune encephalomyelitis by regulating GM-CSF and IL-23 production in dendritic cells. Proc Natl Acad Sci U S A 109(10):3897-902. [PubMed: 22355103]  [MGI Ref ID J:182145]

Schuh JM; Power CA; Proudfoot AE; Kunkel SL; Lukacs NW; Hogaboam CM. 2002. Airway hyperresponsiveness, but not airway remodeling, is attenuated during chronic pulmonary allergic responses to Aspergillus in CCR4-/- mice. FASEB J 16(10):1313-5. [PubMed: 12154006]  [MGI Ref ID J:120221]

Semmling V; Lukacs-Kornek V; Thaiss CA; Quast T; Hochheiser K; Panzer U; Rossjohn J; Perlmutter P; Cao J; Godfrey DI; Savage PB; Knolle PA; Kolanus W; Forster I; Kurts C. 2010. Alternative cross-priming through CCL17-CCR4-mediated attraction of CTLs toward NKT cell-licensed DCs. Nat Immunol 11(4):313-20. [PubMed: 20190758]  [MGI Ref ID J:158986]

Stolberg VR; Chiu BC; Schmidt BM; Kunkel SL; Sandor M; Chensue SW. 2011. CC chemokine receptor 4 contributes to innate NK and chronic stage T helper cell recall responses during Mycobacterium bovis infection. Am J Pathol 178(1):233-44. [PubMed: 21224060]  [MGI Ref ID J:168088]

Stolberg VR; Martin B; Mancuso P; Olszewski MA; Freeman CM; Curtis JL; Chensue SW. 2014. Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure. Am J Pathol 184(2):454-63. [PubMed: 24333113]  [MGI Ref ID J:206301]

Stutte S; Quast T; Gerbitzki N; Savinko T; Novak N; Reifenberger J; Homey B; Kolanus W; Alenius H; Forster I. 2010. Requirement of CCL17 for CCR7- and CXCR4-dependent migration of cutaneous dendritic cells. Proc Natl Acad Sci U S A 107(19):8736-41. [PubMed: 20421491]  [MGI Ref ID J:160297]

Traeger T; Kessler W; Assfalg V; Cziupka K; Koerner P; Dassow C; Breitbach K; Mikulcak M; Steinmetz I; Pfeffer K; Heidecke CD; Maier S. 2008. Detrimental role of CC chemokine receptor 4 in murine polymicrobial sepsis. Infect Immun 76(11):5285-93. [PubMed: 18765730]  [MGI Ref ID J:141175]

Trujillo G; O'Connor EC; Kunkel SL; Hogaboam CM. 2008. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis. Am J Pathol 172(5):1209-21. [PubMed: 18403600]  [MGI Ref ID J:134325]

Tubo NJ; McLachlan JB; Campbell JJ. 2011. Chemokine receptor requirements for epidermal T-cell trafficking. Am J Pathol 178(6):2496-503. [PubMed: 21641376]  [MGI Ref ID J:173299]

Weber C; Meiler S; Doring Y; Koch M; Drechsler M; Megens RT; Rowinska Z; Bidzhekov K; Fecher C; Ribechini E; van Zandvoort MA; Binder CJ; Jelinek I; Hristov M; Boon L; Jung S; Korn T; Lutz MB; Forster I; Zenke M; Hieronymus T; Junt T; Zernecke A. 2011. CCL17-expressing dendritic cells drive atherosclerosis by restraining regulatory T cell homeostasis in mice. J Clin Invest 121(7):2898-910. [PubMed: 21633167]  [MGI Ref ID J:175665]

Yuan Q; Bromley SK; Means TK; Jones KJ; Hayashi F; Bhan AK; Luster AD. 2007. CCR4-dependent regulatory T cell function in inflammatory bowel disease. J Exp Med 204(6):1327-34. [PubMed: 17548518]  [MGI Ref ID J:125857]

Zhang N; Schroppel B; Lal G; Jakubzick C; Mao X; Chen D; Yin N; Jessberger R; Ochando JC; Ding Y; Bromberg JS. 2009. Regulatory T cells sequentially migrate from inflamed tissues to draining lymph nodes to suppress the alloimmune response. Immunity 30(3):458-69. [PubMed: 19303390]  [MGI Ref ID J:147032]

. 1999. Proceedings of the Euroconference of Institute Pasteur. Chemokines and their Receptors: From Basic Research to Therapeutic Intervention. Paris, France, March 11-13, 1999. (Power CA - Effects of CCR4 knockout in a mouse model of lung inflammation, pg. 296-7.) Eur Cytokine Netw 10(2):253-307. [PubMed: 10475688]  [MGI Ref ID J:56464]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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