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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N?+F2 Donating Investigator Steven Reeves, Massachusetts General Hospital Description
Mice that are hemizygous for this transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat nestin intron II enhancer/promoter. According to the donating investigator, expression occurs in the neuroepithelium of the developing nervous system (embryonic days 10-17), in some neuron subsets of the adult mouse (e.g. cerebellum, hippocampal dentate gyrus, subventricular zone, spinal cord) and in adult testes. The rtTA gene is cotranscribed with the lacZ reporter gene Bgeo, which allows verification of the site of rtTA expression. When Tg(Nes-rtTA) hemizygous mice are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a tool that allows the inducible expression of genes in the central nervous system both during early developmental stages and in the adult.Development
A transgenic construct containing a rat nestin intron II enhancer/promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, an IRES sequence and Bgeo gene was injected into FVB/N embryos. Founder mice were bred to FVB/N animals.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (176 strains)
002858 STOCK Tg(Nes-cre)1Wme/J 002859 STOCK Tg(Nes-cre)2Wme/J
005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 007176 B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedNeurobiology Research
lacZ expression in neural tissue
Tet Expression System (tTA/rtTA Expressing Strains)
Research Tools
lacZ Expression
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Genetics Research (Tissue/Cell Markers: neurons)
Genetics Research (Tissue/Cell Markers: testicular cell marker)
Neurobiology Research (Tetop Tet System)
Neurobiology Research (cell marker)
Tet Expression Systems (tTA/rtTA Expressing Strains)
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Nes-rtTA)306Rvs | ||
|---|---|---|---|
| Allele Name | transgene insertion 306, Steven A Reeves | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | tetOp27Kip; | ||
| Mutation Made By | Steven Reeves, Massachusetts General Hospital | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | rtTA and Bgeo are cotranscribed in neuroepithelium of the developing nervous system and in some neuron subsets of the adult mouse. | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | Nes, nestin, mouse, laboratory | ||
| General Note |
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express the rtTA transgene under the control of the rat nestin intron II enhancer/promoter.Expression occurs inthe neuroepithelium of the developing nervous system (embryonic days 10-17), in some neuron subsets of the adult mouse, for example, the cerebellum, hippocampal dentate gyrus, subventricular zone, spinal cord), and in adult testes. When Tg(Nes-rtTA)306Rvs hemizygous mice are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. | ||
| Molecular Note | The transgene contains a rat nestin intron II enhancer/promoter, the reverse tetracycline-controlled transactivator protein (rtTA) gene, an IRES sequence, and a Beta-geo gene. [MGI Ref ID J:69630] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), MCA, vers. 4
Tg(tTA), QPCR, vers. 3
Tg(tTA), STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Mitsuhashi T; Aoki Y; Eksioglu YZ; Takahashi T; Bhide PG; Reeves SA; Caviness VS Jr. 2001. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells. Proc Natl Acad Sci U S A 98(11):6435-40. [PubMed: 11371649] [MGI Ref ID J:69630]
Additional References
Tg(Nes-rtTA)306Rvs relatedCaviness VS Jr; Goto T; Tarui T; Takahashi T; Bhide PG; Nowakowski RS. 2003. Cell output, cell cycle duration and neuronal specification: a model of integrated mechanisms of the neocortical proliferative process. Cereb Cortex 13(6):592-8. [PubMed: 12764033] [MGI Ref ID J:102083]
Farioli-Vecchioli S; Saraulli D; Costanzi M; Pacioni S; Cina I; Aceti M; Micheli L; Bacci A; Cestari V; Tirone F. 2008. The timing of differentiation of adult hippocampal neurons is crucial for spatial memory. PLoS Biol 6(10):e246. [PubMed: 18842068] [MGI Ref ID J:141129]
Rachdi L; Balcazar N; Elghazi L; Barker DJ; Krits I; Kiyokawa H; Bernal-Mizrachi E. 2006. Differential effects of p27 in regulation of beta-cell mass during development, neonatal period, and adult life. Diabetes 55(12):3520-8. [PubMed: 17130500] [MGI Ref ID J:120997]
Suter B; Nowakowski RS; Bhide PG; Caviness VS. 2007. Navigating neocortical neurogenesis and neuronal specification: a positional information system encoded by neurogenetic gradients. J Neurosci 27(40):10777-84. [PubMed: 17913911] [MGI Ref ID J:125593]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain originated on an FVB/N background. It is maintained as a hemizygote on an FVB background. Coat color expected from breeding:Albino
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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