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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(Tek-Cre)12Flv (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N7+12 (04-DEC-07) Donating Investigator Richard Flavell, Yale University School of Medicine Description
These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in the female germline as well as in endothelial cells and hematopoietic cells. A low frequency of deletion events are also observed by inheritance from the male germline.Development
A transgenic construct containing the mouse Tek promoter/enhancer directing expression of a Cre recombinase gene was injected into the fertilized eggs of C3B6F2 mice. Founder animals were obtained and crossed with C57BL/6J mice for at least 7 generations (as of 2001).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tek
008863 B6.Cg-Tg(Tek-cre)1Ywa/J 008537 FVB-Tg(Tek-cre)2352Rwng/J 002856 FVB/N-Tg(TIE2-lacZ)182Sato/J 005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J 006770 STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J 003658 STOCK Tg(TIE2GFP)287Sato/J View Strains carrying other alleles of Tek (6 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Tek-cre)12Flv/0
involves: C3H * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:68147)
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Tek-cre)12Flv | ||
|---|---|---|---|
| Allele Name | transgene insertion 12, Richard A Flavell | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | CreTek; TIE2/cre; TIE2Cre; TIE2Cre+; Tie2-Cre; Tie2-Cre; btie2-cre; | ||
| Mutation Made By | Pandelakis Koni, Medical College of Georgia | ||
| Strain of Origin | (C3H x C57BL/6)F2 | ||
| Site of Expression | female germline as well as tyrosine endothelial and hematopoietic cells; a low frequency of deletion events are also observed by inheritance from the male germline | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Tek-cre)12Flv, transgene insertion 12, Richard A Flavell | ||
| Chromosome | UN | ||
| Gene Common Name(s) | CreTek; TIE2/cre; TIE2Cre; TIE2Cre+; Tie2-Cre; Tie2-Cre; | ||
| Driver Note | Tek | ||
| Molecular Note | Cre recombinase expression is driven by a mouse endothelial-specific receptor tyrosine kinase promoter/enhancer. Recombinase activity is detected in endothelial cells during embryogenesis and adulthood. Expression was also detected in hematopoietic cells. [MGI Ref ID J:68147] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Koni PA; Joshi SK; Temann UA; Olson D; Burkly L; Flavell RA. 2001. Conditional Vascular Cell Adhesion Molecule 1 Deletion in Mice. Impaired lymphocyte migration to bone marrow. J Exp Med 193(6):741-54. [PubMed: 11257140] [MGI Ref ID J:68147]
Additional References
Lincoln J; Alfieri CM; Yutzey KE. 2004. Development of heart valve leaflets and supporting apparatus in chicken and mouse embryos. Dev Dyn 230(2):239-50. [PubMed: 15162503] [MGI Ref ID J:90275]
Tg(Tek-cre)12Flv relatedAra T; Tokoyoda K; Okamoto R; Koni PA; Nagasawa T. 2005. The role of CXCL12 in the organ-specific process of artery formation. Blood 105(8):3155-61. [PubMed: 15626744] [MGI Ref ID J:98453]
Araki T; Chan G; Newbigging S; Morikawa L; Bronson RT; Neel BG. 2009. Noonan syndrome cardiac defects are caused by PTPN11 acting in endocardium to enhance endocardial-mesenchymal transformation. Proc Natl Acad Sci U S A 106(12):4736-41. [PubMed: 19251646] [MGI Ref ID J:147154]
Banerjee ER; Jiang Y; Henderson WR Jr; Scott LM; Papayannopoulou T. 2007. Alpha4 and beta2 integrins have nonredundant roles for asthma development, but for optimal allergen sensitization only alpha4 is critical. Exp Hematol 35(4):605-17. [PubMed: 17379071] [MGI Ref ID J:123141]
Banerjee ER; Latchman YE; Jiang Y; Priestley GV; Papayannopoulou T. 2008. Distinct changes in adult lymphopoiesis in Rag2-/- mice fully reconstituted by alpha4-deficient adult bone marrow cells. Exp Hematol 36(8):1004-13. [PubMed: 18468770] [MGI Ref ID J:139405]
Bazigou E; Xie S; Chen C; Weston A; Miura N; Sorokin L; Adams R; Muro AF; Sheppard D; Makinen T. 2009. Integrin-alpha9 is required for fibronectin matrix assembly during lymphatic valve morphogenesis. Dev Cell 17(2):175-86. [PubMed: 19686679] [MGI Ref ID J:152968]
Beppu H; Malhotra R; Beppu Y; Lepore JJ; Parmacek MS; Bloch KD. 2009. BMP type II receptor regulates positioning of outflow tract and remodeling of atrioventricular cushion during cardiogenesis. Dev Biol 331(2):167-75. [PubMed: 19409885] [MGI Ref ID J:150767]
Berman SD; West JC; Danielian PS; Caron AM; Stone JR; Lees JA. 2009. Mutation of p107 exacerbates the consequences of Rb loss in embryonic tissues and causes cardiac and blood vessel defects. Proc Natl Acad Sci U S A 106(35):14932-6. [PubMed: 19706423] [MGI Ref ID J:153091]
Bonasio R; Scimone ML; Schaerli P; Grabie N; Lichtman AH; von Andrian UH. 2006. Clonal deletion of thymocytes by circulating dendritic cells homing to the thymus. Nat Immunol 7(10):1092-100. [PubMed: 16951687] [MGI Ref ID J:112629]
Brochard V; Combadiere B; Prigent A; Laouar Y; Perrin A; Beray-Berthat V; Bonduelle O; Alvarez-Fischer D; Callebert J; Launay JM; Duyckaerts C; Flavell RA; Hirsch EC; Hunot S. 2009. Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease. J Clin Invest 119(1):182-92. [PubMed: 19104149] [MGI Ref ID J:144702]
Butcher BA; Kim L; Panopoulos AD; Watowich SS; Murray PJ; Denkers EY. 2005. IL-10-independent STAT3 activation by Toxoplasma gondii mediates suppression of IL-12 and TNF-alpha in host macrophages. J Immunol 174(6):3148-52. [PubMed: 15749841] [MGI Ref ID J:97691]
Chen Q; Chen H; Zheng D; Kuang C; Fang H; Zou B; Zhu W; Bu G; Jin T; Wang Z; Zhang X; Chen J; Field LJ; Rubart M; Shou W; Chen Y. 2009. Smad7 is required for the development and function of the heart. J Biol Chem 284(1):292-300. [PubMed: 18952608] [MGI Ref ID J:145777]
Chen ZY; Asavaritikrai P; Prchal JT; Noguchi CT. 2007. Endogenous erythropoietin signaling is required for normal neural progenitor cell proliferation. J Biol Chem 282(35):25875-83. [PubMed: 17604282] [MGI Ref ID J:124657]
Choi EY; Chavakis E; Czabanka MA; Langer HF; Fraemohs L; Economopoulou M; Kundu RK; Orlandi A; Zheng YY; Prieto DA; Ballantyne CM; Constant SL; Aird WC; Papayannopoulou T; Gahmberg CG; Udey MC; Vajkoczy P; Quertermous T; Dimmeler S; Weber C; Chavakis T. 2008. Del-1, an endogenous leukocyte-endothelial adhesion inhibitor, limits inflammatory cell recruitment. Science 322(5904):1101-4. [PubMed: 19008446] [MGI Ref ID J:141063]
Chung Y; Chang SH; Martinez GJ; Yang XO; Nurieva R; Kang HS; Ma L; Watowich SS; Jetten AM; Tian Q; Dong C. 2009. Critical regulation of early Th17 cell differentiation by interleukin-1 signaling. Immunity 30(4):576-87. [PubMed: 19362022] [MGI Ref ID J:147961]
Cohen PA; Koski GK; Czerniecki BJ; Bunting KD; Fu XY; Wang Z; Zhang WJ; Carter CS; Awad M; Distel CA; Nagem H; Paustian CC; Johnson TD; Tisdale JF; Shu S. 2008. STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells. Blood 112(5):1832-43. [PubMed: 18577706] [MGI Ref ID J:138893]
Daneman R; Agalliu D; Zhou L; Kuhnert F; Kuo CJ; Barres BA. 2009. Wnt/beta-catenin signaling is required for CNS, but not non-CNS, angiogenesis. Proc Natl Acad Sci U S A 106(2):641-6. [PubMed: 19129494] [MGI Ref ID J:143865]
El Kasmi KC; Smith AM; Williams L; Neale G; Panopolous A; Watowich SS; Hacker H; Foxwell BM; Murray PJ. 2007. Cutting edge: A transcriptional repressor and corepressor induced by the STAT3-regulated anti-inflammatory signaling pathway. J Immunol 179(11):7215-9. [PubMed: 18025162] [MGI Ref ID J:133009]
Esashi E; Wang YH; Perng O; Qin XF; Liu YJ; Watowich SS. 2008. The signal transducer STAT5 inhibits plasmacytoid dendritic cell development by suppressing transcription factor IRF8. Immunity 28(4):509-20. [PubMed: 18342552] [MGI Ref ID J:134464]
Friedrich EB; Liu E; Sinha S; Cook S; Milstone DS; MacRae CA; Mariotti M; Kuhlencordt PJ; Force T; Rosenzweig A; St-Arnaud R; Dedhar S; Gerszten RE. 2004. Integrin-linked kinase regulates endothelial cell survival and vascular development. Mol Cell Biol 24(18):8134-44. [PubMed: 15340074] [MGI Ref ID J:92970]
Fritz-Six KL; Dunworth WP; Li M; Caron KM. 2008. Adrenomedullin signaling is necessary for murine lymphatic vascular development. J Clin Invest 118(1):40-50. [PubMed: 18097475] [MGI Ref ID J:130930]
Galan-Caridad JM; Harel S; Arenzana TL; Hou ZE; Doetsch FK; Mirny LA; Reizis B. 2007. Zfx controls the self-renewal of embryonic and hematopoietic stem cells. Cell 129(2):345-57. [PubMed: 17448993] [MGI Ref ID J:149654]
Gerety SS; Anderson DJ. 2002. Cardiovascular ephrinB2 function is essential for embryonic angiogenesis. Development 129(6):1397-410. [PubMed: 11880349] [MGI Ref ID J:74909]
Goddeeris MM; Rho S; Petiet A; Davenport CL; Johnson GA; Meyers EN; Klingensmith J. 2008. Intracardiac septation requires hedgehog-dependent cellular contributions from outside the heart. Development 135(10):1887-95. [PubMed: 18441277] [MGI Ref ID J:134643]
Goyama S; Yamamoto G; Shimabe M; Sato T; Ichikawa M; Ogawa S; Chiba S; Kurokawa M. 2008. Evi-1 is a critical regulator for hematopoietic stem cells and transformed leukemic cells. Cell Stem Cell 3(2):207-20. [PubMed: 18682242] [MGI Ref ID J:149794]
Griffin CT; Brennan J; Magnuson T. 2008. The chromatin-remodeling enzyme BRG1 plays an essential role in primitive erythropoiesis and vascular development. Development 135(3):493-500. [PubMed: 18094026] [MGI Ref ID J:130852]
Hamada K; Sasaki T; Koni PA; Natsui M; Kishimoto H; Sasaki J; Yajima N; Horie Y; Hasegawa G; Naito M; Miyazaki J; Suda T; Itoh H; Nakao K; Mak TW; Nakano T; Suzuki A. 2005. The PTEN/PI3K pathway governs normal vascular development and tumor angiogenesis. Genes Dev 19(17):2054-65. [PubMed: 16107612] [MGI Ref ID J:100493]
Hayashi M; Kim SW; Imanaka-Yoshida K; Yoshida T; Abel ED; Eliceiri B; Yang Y; Ulevitch RJ; Lee JD. 2004. Targeted deletion of BMK1/ERK5 in adult mice perturbs vascular integrity and leads to endothelial failure. J Clin Invest 113(8):1138-48. [PubMed: 15085193] [MGI Ref ID J:89215]
Jiao K; Langworthy M; Batts L; Brown CB; Moses HL; Baldwin HS. 2006. Tgfbeta signaling is required for atrioventricular cushion mesenchyme remodeling during in vivo cardiac development. Development 133(22):4585-93. [PubMed: 17050629] [MGI Ref ID J:137594]
Kennedy Norton S; Barnstein B; Brenzovich J; Bailey DP; Kashyap M; Speiran K; Ford J; Conrad D; Watowich S; Moralle MR; Kepley CL; Murray PJ; Ryan JJ. 2008. IL-10 Suppresses Mast Cell IgE Receptor Expression and Signaling In Vitro and In Vivo. J Immunol 180(5):2848-54. [PubMed: 18292506] [MGI Ref ID J:131544]
Kim I; Saunders TL; Morrison SJ. 2007. Sox17 dependence distingushes the transcriptional regulation of fetal from adult hematopoietic stem cells Cell 130(3):470-83. [PubMed: 17655922] [MGI Ref ID J:123050]
Kleaveland B; Zheng X; Liu JJ; Blum Y; Tung JJ; Zou Z; Chen M; Guo L; Lu MM; Zhou D; Kitajewski J; Affolter M; Ginsberg MH; Kahn ML. 2009. Regulation of cardiovascular development and integrity by the heart of glass-cerebral cavernous malformation protein pathway. Nat Med 15(2):169-76. [PubMed: 19151727] [MGI Ref ID J:146528]
Komatsu K; Wakatsuki S; Yamada S; Yamamura K; Miyazaki J; Sehara-Fujisawa A. 2007. Meltrin beta expressed in cardiac neural crest cells is required for ventricular septum formation of the heart. Dev Biol 303(1):82-92. [PubMed: 17126315] [MGI Ref ID J:118804]
Koo BK; Yoon MJ; Yoon KJ; Im SK; Kim YY; Kim CH; Suh PG; Jan YN; Kong YY. 2007. An obligatory role of mind bomb-1 in notch signaling of Mammalian development. PLoS ONE 2(11):e1221. [PubMed: 18043734] [MGI Ref ID J:130369]
Koushik SV; Chen H; Wang J; Conway SJ. 2002. Generation of a conditional loxP allele of the Pax3 transcription factor that enables selective deletion of the homeodomain. Genesis 32(2):114-7. [PubMed: 11857794] [MGI Ref ID J:75127]
Krebs LT; Shutter JR; Tanigaki K; Honjo T; Stark KL; Gridley T. 2004. Haploinsufficient lethality and formation of arteriovenous malformations in Notch pathway mutants. Genes Dev 18(20):2469-73. [PubMed: 15466160] [MGI Ref ID J:93125]
Krenz M; Gulick J; Osinska HE; Colbert MC; Molkentin JD; Robbins J. 2008. Role of ERK1/2 signaling in congenital valve malformations in Noonan syndrome. Proc Natl Acad Sci U S A 105(48):18930-5. [PubMed: 19017799] [MGI Ref ID J:142212]
Laouar Y; Welte T; Fu XY; Flavell RA. 2003. STAT3 is required for Flt3L-dependent dendritic cell differentiation. Immunity 19(6):903-12. [PubMed: 14670306] [MGI Ref ID J:87003]
Lee JS; Yu Q; Shin JT; Sebzda E; Bertozzi C; Chen M; Mericko P; Stadtfeld M; Zhou D; Cheng L; Graf T; MacRae CA; Lepore JJ; Lo CW; Kahn ML. 2006. Klf2 is an essential regulator of vascular hemodynamic forces in vivo. Dev Cell 11(6):845-57. [PubMed: 17141159] [MGI Ref ID J:127228]
Li J; Qu X; Bertram JF. 2009. Endothelial-myofibroblast transition contributes to the early development of diabetic renal interstitial fibrosis in streptozotocin-induced diabetic mice. Am J Pathol 175(4):1380-8. [PubMed: 19729486] [MGI Ref ID J:153054]
Li Z; Chen MJ; Stacy T; Speck NA. 2006. Runx1 function in hematopoiesis is required in cells that express Tek. Blood 107(1):106-10. [PubMed: 16174759] [MGI Ref ID J:125796]
Limbourg FP; Takeshita K; Radtke F; Bronson RT; Chin MT; Liao JK. 2005. Essential role of endothelial Notch1 in angiogenesis. Circulation 111(14):1826-32. [PubMed: 15809373] [MGI Ref ID J:100449]
Malester B; Tong X; Ghiu I; Kontogeorgis A; Gutstein DE; Xu J; Hendricks-Munoz KD; Coetzee WA. 2007. Transgenic expression of a dominant negative K(ATP) channel subunit in the mouse endothelium: effects on coronary flow and endothelin-1 secretion. FASEB J 21(9):2162-72. [PubMed: 17341678] [MGI Ref ID J:134852]
Mazo IB; Honczarenko M; Leung H; Cavanagh LL; Bonasio R; Weninger W; Engelke K; Xia L; McEver RP; Koni PA; Silberstein LE; von Andrian UH. 2005. Bone marrow is a major reservoir and site of recruitment for central memory CD8+ T cells. Immunity 22(2):259-70. [PubMed: 15723813] [MGI Ref ID J:96675]
Nanba D; Kinugasa Y; Morimoto C; Koizumi M; Yamamura H; Takahashi K; Takakura N; Mekada E; Hashimoto K; Higashiyama S. 2006. Loss of HB-EGF in smooth muscle or endothelial cell lineages causes heart malformation. Biochem Biophys Res Commun 350(2):315-21. [PubMed: 17010937] [MGI Ref ID J:116051]
Nurieva R; Yang XO; Martinez G; Zhang Y; Panopoulos AD; Ma L; Schluns K; Tian Q; Watowich SS; Jetten AM; Dong C. 2007. Essential autocrine regulation by IL-21 in the generation of inflammatory T cells. Nature 448(7152):480-3. [PubMed: 17581589] [MGI Ref ID J:123351]
Panopoulos AD; Zhang L; Snow JW; Jones DM; Smith AM; El Kasmi KC; Liu F; Goldsmith MA; Link DC; Murray PJ; Watowich SS. 2006. STAT3 governs distinct pathways in emergency granulopoiesis and mature neutrophils. Blood 108(12):3682-90. [PubMed: 16888100] [MGI Ref ID J:133477]
Potente M; Ghaeni L; Baldessari D; Mostoslavsky R; Rossig L; Dequiedt F; Haendeler J; Mione M; Dejana E; Alt FW; Zeiher AM; Dimmeler S. 2007. SIRT1 controls endothelial angiogenic functions during vascular growth. Genes Dev 21(20):2644-58. [PubMed: 17938244] [MGI Ref ID J:125690]
Priestley GV; Ulyanova T; Papayannopoulou T. 2007. Sustained alterations in biodistribution of stem/progenitor cells in Tie2Cre+ alpha4(f/f) mice are hematopoietic cell autonomous. Blood 109(1):109-11. [PubMed: 16931623] [MGI Ref ID J:127230]
Rudd ML; Tua-Smith A; Straus DB. 2006. Lck SH3 domain function is required for T-cell receptor signals regulating thymocyte development. Mol Cell Biol 26(21):7892-900. [PubMed: 16923964] [MGI Ref ID J:114660]
Sawada N; Salomone S; Kim HH; Kwiatkowski DJ; Liao JK. 2008. Regulation of endothelial nitric oxide synthase and postnatal angiogenesis by Rac1. Circ Res 103(4):360-8. [PubMed: 18599867] [MGI Ref ID J:152655]
Shaw AT; Meissner A; Dowdle JA; Crowley D; Magendantz M; Ouyang C; Parisi T; Rajagopal J; Blank LJ; Bronson RT; Stone JR; Tuveson DA; Jaenisch R; Jacks T. 2007. Sprouty-2 regulates oncogenic K-ras in lung development and tumorigenesis. Genes Dev 21(6):694-707. [PubMed: 17369402] [MGI Ref ID J:119477]
Shen TL; Park AY; Alcaraz A; Peng X; Jang I; Koni P; Flavell RA; Gu H; Guan JL. 2005. Conditional knockout of focal adhesion kinase in endothelial cells reveals its role in angiogenesis and vascular development in late embryogenesis. J Cell Biol 169(6):941-52. [PubMed: 15967814] [MGI Ref ID J:99637]
Shim JH; Xiao C; Hayden MS; Lee KY; Trombetta ES; Pypaert M; Nara A; Yoshimori T; Wilm B; Erdjument-Bromage H; Tempst P; Hogan BL; Mellman I; Ghosh S. 2006. CHMP5 is essential for late endosome function and down-regulation of receptor signaling during mouse embryogenesis. J Cell Biol 172(7):1045-56. [PubMed: 16567502] [MGI Ref ID J:107913]
Shimoda M; Li T; Pihkala JP; Koni PA. 2006. Role of MHC class II on memory B cells in post-germinal center B cell homeostasis and memory response. J Immunol 176(4):2122-33. [PubMed: 16455968] [MGI Ref ID J:129125]
Shimoda M; Mmanywa F; Joshi SK; Li T; Miyake K; Pihkala J; Abbas JA; Koni PA. 2006. Conditional ablation of MHC-II suggests an indirect role for MHC-II in regulatory CD4 T cell maintenance. J Immunol 176(11):6503-11. [PubMed: 16709807] [MGI Ref ID J:131774]
Sierro F; Biben C; Martinez-Munoz L; Mellado M; Ransohoff RM; Li M; Woehl B; Leung H; Groom J; Batten M; Harvey RP; Martinez-A C; Mackay CR; Mackay F. 2007. Disrupted cardiac development but normal hematopoiesis in mice deficient in the second CXCL12/SDF-1 receptor, CXCR7. Proc Natl Acad Sci U S A 104(37):14759-64. [PubMed: 17804806] [MGI Ref ID J:124878]
Sinclair GB; Jevon G; Colobong KE; Randall DR; Choy FY; Clarke LA. 2007. Generation of a conditional knockout of murine glucocerebrosidase: Utility for the study of Gaucher disease. Mol Genet Metab 90(2):148-56. [PubMed: 17079175] [MGI Ref ID J:117763]
Speiran K; Bailey DP; Fernando J; Macey M; Barnstein B; Kolawole M; Curley D; Watowich SS; Murray PJ; Oskeritzian C; Ryan JJ. 2009. Endogenous suppression of mast cell development and survival by IL-4 and IL-10. J Leukoc Biol 85(5):826-36. [PubMed: 19228815] [MGI Ref ID J:149777]
Sridurongrit S; Larsson J; Schwartz R; Ruiz-Lozano P; Kaartinen V. 2008. Signaling via the Tgf-beta type I receptor Alk5 in heart development. Dev Biol 322(1):208-18. [PubMed: 18718461] [MGI Ref ID J:142286]
Suzuki A; Nakano T; Mak TW; Sasaki T. 2008. Portrait of PTEN: messages from mutant mice. Cancer Sci 99(2):209-13. [PubMed: 18201277] [MGI Ref ID J:142484]
Takeshita K; Satoh M; Ii M; Silver M; Limbourg FP; Mukai Y; Rikitake Y; Radtke F; Gridley T; Losordo DW; Liao JK. 2007. Critical role of endothelial Notch1 signaling in postnatal angiogenesis. Circ Res 100(1):70-8. [PubMed: 17158336] [MGI Ref ID J:130243]
Tan W; Palmby TR; Gavard J; Amornphimoltham P; Zheng Y; Gutkind JS. 2008. An essential role for Rac1 in endothelial cell function and vascular development. FASEB J 22(6):1829-38. [PubMed: 18245172] [MGI Ref ID J:136526]
Ulyanova T; Priestley GV; Nakamoto B; Jiang Y; Papayannopoulou T. 2007. VCAM-1 ablation in nonhematopoietic cells in MxCre+ VCAM-1f/f mice is variable and dictates their phenotype. Exp Hematol 35(4):565-71. [PubMed: 17379066] [MGI Ref ID J:123142]
Ulyanova T; Scott LM; Priestley GV; Jiang Y; Nakamoto B; Koni PA; Papayannopoulou T. 2005. VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin. Blood 106(1):86-94. [PubMed: 15769895] [MGI Ref ID J:107447]
Venkatesh DA; Park KS; Harrington A; Miceli-Libby L; Yoon JK; Liaw L. 2008. Cardiovascular and hematopoietic defects associated with Notch1 activation in embryonic Tie2-expressing populations. Circ Res 103(4):423-31. [PubMed: 18617694] [MGI Ref ID J:152652]
Walisser JA; Glover E; Pande K; Liss AL; Bradfield CA. 2005. Aryl hydrocarbon receptor-dependent liver development and hepatotoxicity are mediated by different cell types. Proc Natl Acad Sci U S A 102(49):17858-63. [PubMed: 16301529] [MGI Ref ID J:104388]
Wang J; Sridurongrit S; Dudas M; Thomas P; Nagy A; Schneider MD; Epstein JA; Kaartinen V. 2005. Atrioventricular cushion transformation is mediated by ALK2 in the developing mouse heart. Dev Biol 286(1):299-310. [PubMed: 16140292] [MGI Ref ID J:103532]
Wang M; Zhang W; Crisostomo P; Markel T; Meldrum KK; Fu XY; Meldrum DR. 2007. STAT3 mediates bone marrow mesenchymal stem cell VEGF production. J Mol Cell Cardiol 42(6):1009-15. [PubMed: 17509611] [MGI Ref ID J:123061]
Wang M; Zhang W; Crisostomo P; Markel T; Meldrum KK; Fu XY; Meldrum DR. 2007. Sex differences in endothelial STAT3 mediate sex differences in myocardial inflammation. Am J Physiol Endocrinol Metab 293(3):E872-7. [PubMed: 17595217] [MGI Ref ID J:125531]
Welte T; Zhang SS; Wang T; Zhang Z; Hesslein DG; Yin Z; Kano A; Iwamoto Y; Li E; Craft JE; Bothwell AL; Fikrig E; Koni PA; Flavell RA; Fu XY. 2003. STAT3 deletion during hematopoiesis causes Crohn's disease-like pathogenesis and lethality: a critical role of STAT3 in innate immunity. Proc Natl Acad Sci U S A 100(4):1879-84. [PubMed: 12571365] [MGI Ref ID J:81973]
Xie J; Wu T; Xu K; Huang IK; Cleaver O; Huang CL. 2009. Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice. Am J Pathol 175(3):1315-27. [PubMed: 19644017] [MGI Ref ID J:152906]
Xiong Y; Mahmood A; Lu D; Qu C; Kazmi H; Goussev A; Zhang ZG; Noguchi CT; Schallert T; Chopp M. 2008. Histological and functional outcomes after traumatic brain injury in mice null for the erythropoietin receptor in the central nervous system. Brain Res 1230:247-57. [PubMed: 18657521] [MGI Ref ID J:139962]
Yamazaki T; Yang XO; Chung Y; Fukunaga A; Nurieva R; Pappu B; Martin-Orozco N; Kang HS; Ma L; Panopoulos AD; Craig S; Watowich SS; Jetten AM; Tian Q; Dong C. 2008. CCR6 regulates the migration of inflammatory and regulatory T cells. J Immunol 181(12):8391-401. [PubMed: 19050256] [MGI Ref ID J:142071]
Yeh JR; Ju R; Brdlik CM; Zhang W; Zhang Y; Matyskiela ME; Shotwell JD; Crews CM. 2006. Targeted gene disruption of methionine aminopeptidase 2 results in an embryonic gastrulation defect and endothelial cell growth arrest. Proc Natl Acad Sci U S A 103(27):10379-84. [PubMed: 16790550] [MGI Ref ID J:111701]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated on a C3B6F2 background and has been backcrossed to C57BL/6J for at least seven generations (7/01). Coat color expected from breeding:Black Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $239.00 Female or Male Hemizygous for Tg(Tek-cre)12Flv
Pairs /Price (US dollars $) Pair Genotype $293.35 Hemizygous for Tg(Tek-cre)12Flv x Noncarrier $293.35 Noncarrier x Hemizygous for Tg(Tek-cre)12Flv $478.00 Hemizygous for Tg(Tek-cre)12Flv x Hemizygous for Tg(Tek-cre)12Flv
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $310.70 Female or Male Hemizygous for Tg(Tek-cre)12Flv
Pairs /Price (US dollars $) Pair Genotype $381.40 Hemizygous for Tg(Tek-cre)12Flv x Noncarrier $381.40 Noncarrier x Hemizygous for Tg(Tek-cre)12Flv $621.40 Hemizygous for Tg(Tek-cre)12Flv x Hemizygous for Tg(Tek-cre)12Flv
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
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| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.