Strain Name: |
B6.Cg-Tg(Tek-cre)12Flv/J |
|---|---|
Stock Number: |
004128 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(Tek-Cre)12Flv (Changed: 15-DEC-04
) |
| Genes & Alleles | Tek; cre; Tg(Tek-cre)12Flv; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Richard Flavell, Yale University School of Medicine Generation N7+12 (04-DEC-07) Strain Description
These transgenic mice express Cre recombinase under the direction of the receptor tyrosine kinase Tek promoter/enhancer, which has been shown to provide uniform expression in endothelial cells during embryogenesis and adulthood. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity results in the deletion of loxP flanked targets in the female germline as well as in endothelial cells and hematopoietic cells. A low frequency of deletion events are also observed by inheritance from the male germline.Strain Development
A transgenic construct containing the mouse Tek promoter/enhancer directing expression of a Cre recombinase gene was injected into the fertilized eggs of C3B6F2 mice. Founder animals were obtained and crossed with C57BL/6J mice for at least 7 generations (as of 2001).
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Tek-cre)12Flv | ||
|---|---|---|---|
| Allele Name | transgene insertion 12, Richard A Flavell | ||
| Common Name(s) | CreTek; TIE2/cre; TIE2Cre; TIE2Cre+; Tie2-Cre; Tie2-Cre; | ||
| Mutation Made By | Pandelakis Koni, Medical College of Georgia | ||
| Strain of Origin | (C3H x C57BL/6)F2 | ||
| Site of Expression | female germline as well as tyrosine endothelial and hematopoietic cells; a low frequency of deletion events are also observed by inheritance from the male germline | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| Molecular Note | Cre recombinase expression is driven by a mouse endothelial-specific receptor tyrosine kinase promoter/enhancer. Recombinase activity is detected in endothelial cells during embryogenesis and adulthood. Expression was also detected in hematopoietic cells. [J:68147] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(balancer2)3Cgn(Cre), Tg(Sycp1-Cre)4Min, Tg(AlbCre)21Mgn, Tg(Ins2Cre)25Mgn, Tg(Mx1-Cre)1Cgn
Colony Maintenance
| Breeding & Husbandry | This strain originated on a C3B6F2 background and has been backcrossed to C57BL/6J for at least seven generations (7/01). Coat color expected from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Tek
004659 B6.Cg-Tg(TIE2GFP)287Sato/1J 002856 FVB/N-Tg(TIE2-lacZ)182Sato/J 005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J 003658 STOCK Tg(TIE2GFP)287Sato/J View Strains carrying other alleles of Tek (4 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox-System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox system)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Immunology and Inflammation Research
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesKoni PA; Joshi SK; Temann UA; Olson D; Burkly L; Flavell RA. 2001. Conditional Vascular Cell Adhesion Molecule 1 Deletion in Mice. Impaired lymphocyte migration to bone marrow. J Exp Med 193(6):741-54. [PubMed: 11257140] [J:68147]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Tek-cre)12Flv/J |
| Stock Number: | 004128 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of this STRAIN, including cross-bred progeny, by for-profit entities or for Commercial Purposes requires a license prior to shipping
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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