Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+F1p (17-APR-05) Generation Definitions   Donating Investigator Dr. Jurrien Dean,   NIH

Description
Mice that are homozygous null for the Zp2 gene are viable, normal in size and do not display any gross physical abnormalities. No Zp2 gene protein product is immunodetectable in ovary tissue. Although the ovaries appear normal with follicles at all stages of development present, homozygous females are infertile. A thin zona matrix is present in early follicles but is not sustained in pre-ovulatory follicles. The abnormal zona matrix does not affect initial folliculogenesis, but there is a significant decrease in the number of antral stage follicles in ovaries isolated from mice lacking a zona pellucida. Few eggs are detected in the oviduct after stimulation with gonadotropins, and no two-cell embryos are recovered after mating Zp2-null females with normal male mice. Although zona-free oocytes mature and can be fertilized in vitro and successfully progress to the blastocyst stage, the developmental potential of blastocysts derived from homozygous Zp2-null eggs appears compromised. The donating investigator has not observed live births when fertilized null eggs are transferred to foster mothers. Male homozygotes are fertile and phenotypically normal.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a 5' region of the Zp2 gene (exons 1-2). The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6N blastocysts. The resulting chimeric animals were crossed to outbred CF1 mice.

Control Information

	Control
	Heterozygote from the colony
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Zp2^tm1Dean/Zp2^tm1Dean

        involves: 129S1/Sv * 129X1/SvJ * CF-1

• endocrine/exocrine gland phenotype
• abnormal cumulus oophorus
  • disorganized cumulus-oocyte-complex   (MGI Ref ID J:67817)
• abnormal secondary ovarian follicle morphology
  • reduced number of antral follicles relative to wild-type   (MGI Ref ID J:67817)
• reproductive system phenotype
• abnormal cumulus oophorus
  • disorganized cumulus-oocyte-complex   (MGI Ref ID J:67817)
• abnormal ovulation
  • the number of eggs ovulated was <5% that in wild-type mice   (MGI Ref ID J:67817)
• abnormal secondary ovarian follicle morphology
  • reduced number of antral follicles relative to wild-type   (MGI Ref ID J:67817)
• female infertility   (MGI Ref ID J:67817)
• thin zona pellucida
  • a thin zona pellucida is observed in pre-antral follicles, but not in the later stages of folliculogenesis   (MGI Ref ID J:67817)
• cellular phenotype
• abnormal cumulus oophorus
  • disorganized cumulus-oocyte-complex   (MGI Ref ID J:67817)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Zp2^tm1Dean related

Reproductive Biology Research
Fertility Defects
      females only

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Zp2tm1Dean
Allele Name		targeted mutation 1 Jurrien Dean
Allele Type		Targeted (knock-out)
Common Name(s)		Zp2tm1Nih; Zp2tm2Nih; Zp2tm;
Mutation Made By		Dr. Jurrien Dean,   NIH
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Zp2, zona pellucida glycoprotein 2
Chromosome		7
Gene Common Name(s)		ZPA; Zp-2;
Molecular Note		Part of exon 1 and exon 2 were replaced by a neomycin cassette. Absence of the protein was confirmed by immunohistochemistry on ovarian sections of mutant female mice. [MGI Ref ID J:67817]

Genotyping

Genotyping Information

Genotyping Protocols

Zp2^tm1Dean, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Rankin TL; O'Brien M; Lee E; Wigglesworth K; Eppig J; Dean J. 2001. Defective zonae pellucidae in Zp2-null mice disrupt folliculogenesis, fertility and development. Development 128(7):1119-26. [PubMed: 11245577]  [MGI Ref ID J:67817]

Additional References

Zp2^tm1Dean related

Choi Y; Rajkovic A. 2006. Genetics of early mammalian folliculogenesis. Cell Mol Life Sci 63(5):579-90. [PubMed: 16416028]  [MGI Ref ID J:108336]

Gahlay G; Gauthier L; Baibakov B; Epifano O; Dean J. 2010. Gamete recognition in mice depends on the cleavage status of an egg's zona pellucida protein. Science 329(5988):216-9. [PubMed: 20616279]  [MGI Ref ID J:161507]

Naz RK; Rajesh C. 2005. Gene knockouts that cause female infertility: search for novel contraceptive targets Front Biosci 10:2447-2459. [PubMed: 15970507]  [MGI Ref ID J:103183]

Rankin TL; Coleman JS; Epifano O; Hoodbhoy T; Turner SG; Castle PE; Lee E; Gore-Langton R; Dean J. 2003. Fertility and taxon-specific sperm binding persist after replacement of mouse sperm receptors with human homologs. Dev Cell 5(1):33-43. [PubMed: 12852850]  [MGI Ref ID J:93482]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129 background and was crossed to outbred CF1 mice. When held in a live colony, they may be maintained by crossing homozygous males with heterozygous females.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Heterozygote from the colony
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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