Strain Name:

B6.129S7-Apptm1Dbo/J

Stock Number:

004133

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Availability:

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Use Restrictions Apply, see Terms of Use
Homozygous App mice weigh 15%-20% less than age-matched wild-type controls, and exhibit decreased locomotor activity and forelimb grip strength. Reactive gliosis is seen in some homozygous mice at 14 weeks of age. This beta-amyloid precursor protein (APP) knock-out strain offers a model useful in studies related to Alzheimer's disease.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
GenerationN10+N8F4 (26-NOV-14)
Generation Definitions
 
Donating InvestigatorDr. David R Borchelt,   University of Florida

Description
At birth, mice homozygous for the targeted allele are viable, and do not display any gross physical or behavioral abnormalities. No App gene product (mRNA or protein) is detected. Body weight is 15-20% less than that observed in wildtype age-matched control mice. By 14 weeks of age the mice exhibit evidence of reactive gliosis. Neurological evaluation reveals significantly reduced forelimb grip strength and decreased locomotor activity. This mutant strain offers a model useful in studies related to Alzheimer's disease.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the App gene encoding the promoter and exon 1. The construct was electroporated into 129S7/SvEvBrd-derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

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View Alzheimer's Disease Models     (109 strains)

Strains carrying other alleles of App
008390   STOCK Apptm1Sud/J
View Strains carrying other alleles of App     (1 strain)

Additional Web Information

Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Alzheimer Disease; AD
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Cerebral Amyloid Angiopathy, App-Related   (APP)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Apptm1Dbo/Apptm1Dbo

        involves: 129S7/SvEvBrd * C57BL/6J
  • behavior/neurological phenotype
  • abnormal active avoidance behavior
    • age-related impairment in conditioned avoidance tests, seen at 10 months of age, but not at 4 months of age   (MGI Ref ID J:53824)
  • abnormal locomotor activation
    • decreased locomotor activity   (MGI Ref ID J:25512)
    • hypoactivity   (MGI Ref ID J:53824)
  • abnormal spatial learning
    • impairment in watermaze test of spatial learning, both at 4 and 10 months of age   (MGI Ref ID J:53824)
  • decreased grip strength
    • significantly reduced grip strength   (MGI Ref ID J:25512)
  • nervous system phenotype
  • abnormal long term potentiation
    • impairment of ability of high frequency stimuli to induce LTP which correlated with extent of gliosis in stratum radiatum   (MGI Ref ID J:53824)
  • abnormal neuron morphology
    • the branching of dendrites of both cortical and hippocampal neurons was much less extensive, however did not show any loss of cells in the cortex or the hippocampus   (MGI Ref ID J:53824)
  • gliosis
    • reactive gliosis was observed throughout the cortical layers of the neocortex and extensive astrogliosis was seen in the CA1 region of the hippocampus, however did not observe neuronal cell damage   (MGI Ref ID J:25512)
    • astrocytosis
      • reactive astrocytosis in many brain areas, but predominantly in the cortex and hippocampus at 14 weeks of age   (MGI Ref ID J:53824)
  • growth/size/body phenotype
  • decreased body weight
    • body weight was 15-20% less at all ages compared with that of controls   (MGI Ref ID J:25512)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Apptm1Dbo related

Neurobiology Research
Alzheimer's Disease
Neurodegeneration

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Apptm1Dbo
Allele Name targeted mutation 1, David R Borchelt
Allele Type Targeted (Null/Knockout)
Common Name(s) App-;
Mutation Made ByDr. David Borchelt,   University of Florida
Strain of Origin129S7/SvEvBrd-Hprt
ES Cell Line NameAB2.1
ES Cell Line Strain129S7/SvEvBrd-Hprt
Gene Symbol and Name App, amyloid beta (A4) precursor protein
Chromosome 16
Gene Common Name(s) AAA; ABETA; ABPP; AD1; AL024401; APPI; Adap; Alzheimer disease amyloid beta protein; CTFgamma; CVAP; Cvap; E030013M08Rik; PN-II; PN2; RIKEN cDNA E030013M08 gene; appican; betaAPP; cerebrovascular amyloid peptide; expressed sequence AL024401; protease nexin II;
Molecular Note A neomycin resistance cassette replaced a 3.8 kb sequence including the promoter region and first exon. Northern blot analysis did not detect mRNA in brain or kidney of homozygous mutant mice. [MGI Ref ID J:25512]

Genotyping

Genotyping Information

Genotyping Protocols

Apptm1 Dbo MCA SEP,

MELT


Apptm1Dbo, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zheng H; Jiang M; Trumbauer ME; Sirinathsinghji DJ; Hopkins R; Smith DW; Heavens RP; Dawson GR; Boyce S; Conner MW; Stevens KA; Slunt HH; Sisodia SS; Chen HY; Van der Ploeg LHT. 1995. beta-Amyloid precursor protein-deficient mice show reactive gliosis and decreased locomotor activity. Cell 81(4):525-31. [PubMed: 7758106]  [MGI Ref ID J:25512]

Additional References

von Koch CS; Zheng H; Chen H; Trumbauer M; Thinakaran G; van der Ploeg LH; Price DL; Sisodia SS. 1997. Generation of APLP2 KO mice and early postnatal lethality in APLP2/APP double KO mice. Neurobiol Aging 18(6):661-9. [PubMed: 9461064]  [MGI Ref ID J:45851]

Apptm1Dbo related

Austin SA; Combs CK. 2010. Amyloid precursor protein mediates monocyte adhesion in AD tissue and apoE(-)/(-) mice. Neurobiol Aging 31(11):1854-66. [PubMed: 19058878]  [MGI Ref ID J:165242]

Barbagallo AP; Wang Z; Zheng H; D'Adamio L. 2011. A single tyrosine residue in the amyloid precursor protein intracellular domain is essential for developmental function. J Biol Chem 286(11):8717-21. [PubMed: 21266574]  [MGI Ref ID J:170948]

Barbagallo AP; Wang Z; Zheng H; D'Adamio L. 2011. The intracellular threonine of amyloid precursor protein that is essential for docking of Pin1 is dispensable for developmental function. PLoS One 6(3):e18006. [PubMed: 21445342]  [MGI Ref ID J:171673]

Bellingham SA; Ciccotosto GD; Needham BE; Fodero LR; White AR; Masters CL; Cappai R; Camakaris J. 2004. Gene knockout of amyloid precursor protein and amyloid precursor-like protein-2 increases cellular copper levels in primary mouse cortical neurons and embryonic fibroblasts. J Neurochem 91(2):423-8. [PubMed: 15447675]  [MGI Ref ID J:93279]

Bryson JB; Hobbs C; Parsons MJ; Bosch KD; Pandraud A; Walsh FS; Doherty P; Greensmith L. 2012. Amyloid precursor protein (APP) contributes to pathology in the SOD1G93A mouse model of amyotrophic lateral sclerosis. Hum Mol Genet 21(17):3871-82. [PubMed: 22678056]  [MGI Ref ID J:185980]

Cappai R; Cheng F; Ciccotosto GD; Needham BE; Masters CL; Multhaup G; Fransson LA; Mani K. 2005. The amyloid precursor protein (APP) of Alzheimer disease and its paralog, APLP2, modulate the Cu/Zn-Nitric Oxide-catalyzed degradation of glypican-1 heparan sulfate in vivo. J Biol Chem 280(14):13913-20. [PubMed: 15677459]  [MGI Ref ID J:98747]

Cataldo AM; Petanceska S; Peterhoff CM; Terio NB; Epstein CJ; Villar A; Carlson EJ; Staufenbiel M; Nixon RA. 2003. App gene dosage modulates endosomal abnormalities of Alzheimer's disease in a segmental trisomy 16 mouse model of down syndrome. J Neurosci 23(17):6788-92. [PubMed: 12890772]  [MGI Ref ID J:84685]

Chen AC; Guo LY; Ostaszewski BL; Selkoe DJ; LaVoie MJ. 2010. Aph-1 associates directly with full-length and C-terminal fragments of gamma-secretase substrates. J Biol Chem 285(15):11378-91. [PubMed: 20145246]  [MGI Ref ID J:161717]

Choi HY; Liu Y; Tennert C; Sugiura Y; Karakatsani A; Kroger S; Johnson EB; Hammer RE; Lin W; Herz J. 2013. APP interacts with LRP4 and agrin to coordinate the development of the neuromuscular junction in mice. Elife 2:e00220. [PubMed: 23986861]  [MGI Ref ID J:207796]

Choi SH; Veeraraghavalu K; Lazarov O; Marler S; Ransohoff RM; Ramirez JM; Sisodia SS. 2008. Non-cell-autonomous effects of presenilin 1 variants on enrichment-mediated hippocampal progenitor cell proliferation and differentiation. Neuron 59(4):568-80. [PubMed: 18760694]  [MGI Ref ID J:149876]

Corrigan F; Vink R; Blumbergs PC; Masters CL; Cappai R; van den Heuvel C. 2012. Characterisation of the effect of knockout of the amyloid precursor protein on outcome following mild traumatic brain injury. Brain Res 1451:87-99. [PubMed: 22424792]  [MGI Ref ID J:183235]

Dawson GR; Seabrook GR; Zheng H; Smith DW; Graham S; O'Dowd G; Bowery BJ; Boyce S; Trumbauer ME; Chen HY; Van der Ploeg LH; Sirinathsinghji DJ. 1999. Age-related cognitive deficits, impaired long-term potentiation and reduction in synaptic marker density in mice lacking the beta-amyloid precursor protein. Neuroscience 90(1):1-13. [PubMed: 10188929]  [MGI Ref ID J:53824]

Dolev I; Fogel H; Milshtein H; Berdichevsky Y; Lipstein N; Brose N; Gazit N; Slutsky I. 2013. Spike bursts increase amyloid-beta 40/42 ratio by inducing a presenilin-1 conformational change. Nat Neurosci 16(5):587-95. [PubMed: 23563578]  [MGI Ref ID J:197603]

Duce JA; Tsatsanis A; Cater MA; James SA; Robb E; Wikhe K; Leong SL; Perez K; Johanssen T; Greenough MA; Cho HH; Galatis D; Moir RD; Masters CL; McLean C; Tanzi RE; Cappai R; Barnham KJ; Ciccotosto GD; Rogers JT; Bush AI. 2010. Iron-export ferroxidase activity of beta-amyloid precursor protein is inhibited by zinc in Alzheimer's disease. Cell 142(6):857-67. [PubMed: 20817278]  [MGI Ref ID J:167925]

Ghosal K; Stathopoulos A; Pimplikar SW. 2010. APP intracellular domain impairs adult neurogenesis in transgenic mice by inducing neuroinflammation. PLoS One 5(7):e11866. [PubMed: 20689579]  [MGI Ref ID J:163067]

Guo Q; Li H; Gaddam SS; Justice NJ; Robertson CS; Zheng H. 2012. Amyloid precursor protein revisited: neuron-specific expression and highly stable nature of soluble derivatives. J Biol Chem 287(4):2437-45. [PubMed: 22144675]  [MGI Ref ID J:181521]

Han P; Dou F; Li F; Zhang X; Zhang YW; Zheng H; Lipton SA; Xu H; Liao FF. 2005. Suppression of cyclin-dependent kinase 5 activation by amyloid precursor protein: a novel excitoprotective mechanism involving modulation of tau phosphorylation. J Neurosci 25(50):11542-52. [PubMed: 16354912]  [MGI Ref ID J:103996]

Hendrickx A; Pierrot N; Tasiaux B; Schakman O; Brion JP; Kienlen-Campard P; De Smet C; Octave JN. 2013. Epigenetic induction of EGR-1 expression by the amyloid precursor protein during exposure to novelty. PLoS One 8(9):e74305. [PubMed: 24066134]  [MGI Ref ID J:206037]

Ho T; Vessey KA; Cappai R; Dinet V; Mascarelli F; Ciccotosto GD; Fletcher EL. 2012. Amyloid precursor protein is required for normal function of the rod and cone pathways in the mouse retina. PLoS One 7(1):e29892. [PubMed: 22279552]  [MGI Ref ID J:184236]

Hoe HS; Fu Z; Makarova A; Lee JY; Lu C; Feng L; Pajoohesh-Ganji A; Matsuoka Y; Hyman BT; Ehlers MD; Vicini S; Pak DT; Rebeck GW. 2009. The effects of amyloid precursor protein on postsynaptic composition and activity. J Biol Chem 284(13):8495-506. [PubMed: 19164281]  [MGI Ref ID J:148737]

Hoe HS; Lee KJ; Carney RS; Lee J; Markova A; Lee JY; Howell BW; Hyman BT; Pak DT; Bu G; Rebeck GW. 2009. Interaction of reelin with amyloid precursor protein promotes neurite outgrowth. J Neurosci 29(23):7459-73. [PubMed: 19515914]  [MGI Ref ID J:149815]

Kamal A; Almenar-Queralt A; LeBlanc JF; Roberts EA; Goldstein LS. 2001. Kinesin-mediated axonal transport of a membrane compartment containing beta-secretase and presenilin-1 requires APP. Nature 414(6864):643-8. [PubMed: 11740561]  [MGI Ref ID J:73257]

Koike MA; Lin AJ; Pham J; Nguyen E; Yeh JJ; Rahimian R; Tromberg BJ; Choi B; Green KN; LaFerla FM. 2012. APP knockout mice experience acute mortality as the result of ischemia. PLoS One 7(8):e42665. [PubMed: 22912719]  [MGI Ref ID J:189875]

Laky K; Annaert W; Fowlkes BJ. 2009. Amyloid precursor family proteins are expressed by thymic and lymph node stromal cells but are not required for lymphocyte development. Int Immunol 21(10):1163-74. [PubMed: 19710207]  [MGI Ref ID J:153769]

Lee KJ; Moussa CE; Lee Y; Sung Y; Howell BW; Turner RS; Pak DT; Hoe HS. 2010. Beta amyloid-independent role of amyloid precursor protein in generation and maintenance of dendritic spines. Neuroscience 169(1):344-56. [PubMed: 20451588]  [MGI Ref ID J:165233]

Li H; Wang Z; Wang B; Guo Q; Dolios G; Tabuchi K; Hammer RE; Sudhof TC; Wang R; Zheng H. 2010. Genetic dissection of the amyloid precursor protein in developmental function and amyloid pathogenesis. J Biol Chem 285(40):30598-605. [PubMed: 20693289]  [MGI Ref ID J:166379]

Linde CI; Baryshnikov SG; Mazzocco-Spezzia A; Golovina VA. 2011. Dysregulation of Ca2+ signaling in astrocytes from mice lacking amyloid precursor protein. Am J Physiol Cell Physiol 300(6):C1502-12. [PubMed: 21368296]  [MGI Ref ID J:174250]

Liu Q; Zerbinatti CV; Zhang J; Hoe HS; Wang B; Cole SL; Herz J; Muglia L; Bu G. 2007. Amyloid precursor protein regulates brain apolipoprotein E and cholesterol metabolism through lipoprotein receptor LRP1. Neuron 56(1):66-78. [PubMed: 17920016]  [MGI Ref ID J:126954]

Ma QH; Futagawa T; Yang WL; Jiang XD; Zeng L; Takeda Y; Xu RX; Bagnard D; Schachner M; Furley AJ; Karagogeos D; Watanabe K; Dawe GS; Xiao ZC. 2008. A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis. Nat Cell Biol 10(3):283-94. [PubMed: 18278038]  [MGI Ref ID J:145671]

Megill A; Lee T; DiBattista AM; Song JM; Spitzer MH; Rubinshtein M; Habib LK; Capule CC; Mayer M; Turner RS; Kirkwood A; Yang J; Pak DT; Lee HK; Hoe HS. 2013. A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis. J Neurosci 33(22):9306-18. [PubMed: 23719799]  [MGI Ref ID J:198662]

Morales-Corraliza J; Schmidt SD; Mazzella MJ; Berger JD; Wilson DA; Wesson DW; Jucker M; Levy E; Nixon RA; Mathews PM. 2013. Immunization targeting a minor plaque constituent clears beta-amyloid and rescues behavioral deficits in an Alzheimer's disease mouse model. Neurobiol Aging 34(1):137-45. [PubMed: 22608241]  [MGI Ref ID J:191360]

Needham B; Wlodek M; Ciccotosto G; Fam B; Masters C; Proietto J; Andrikopoulos S; Cappai R. 2008. Identification of the Alzheimer's disease amyloid precursor protein (APP) and its homologue APLP2 as essential modulators of glucose and insulin homeostasis and growth. J Pathol 215(2):155-63. [PubMed: 18393365]  [MGI Ref ID J:134311]

Nunes A; Pressey SN; Cooper JD; Soriano S. 2011. Loss of amyloid precursor protein in a mouse model of Niemann-Pick type C disease exacerbates its phenotype and disrupts tau homeostasis. Neurobiol Dis 42(3):349-59. [PubMed: 21303697]  [MGI Ref ID J:172769]

Phinney AL; Calhoun ME; Wolfer DP; Lipp HP; Zheng H; Jucker M. 1999. No hippocampal neuron or synaptic bouton loss in learning-impaired aged beta-amyloid precursor protein-null mice. Neuroscience 90(4):1207-16. [PubMed: 10338291]  [MGI Ref ID J:57197]

Pramatarova A; Chen K; Howell BW. 2008. A genetic interaction between the APP and Dab1 genes influences brain development. Mol Cell Neurosci 37(1):178-86. [PubMed: 18029196]  [MGI Ref ID J:132599]

Puig KL; Floden AM; Adhikari R; Golovko MY; Combs CK. 2012. Amyloid precursor protein and proinflammatory changes are regulated in brain and adipose tissue in a murine model of high fat diet-induced obesity. PLoS One 7(1):e30378. [PubMed: 22276186]  [MGI Ref ID J:184299]

Salehi A; Delcroix JD; Belichenko PV; Zhan K; Wu C; Valletta JS; Takimoto-Kimura R; Kleschevnikov AM; Sambamurti K; Chung PP; Xia W; Villar A; Campbell WA; Kulnane LS; Nixon RA; Lamb BT; Epstein CJ; Stokin GB; Goldstein LS; Mobley WC. 2006. Increased App expression in a mouse model of Down's syndrome disrupts NGF transport and causes cholinergic neuron degeneration. Neuron 51(1):29-42. [PubMed: 16815330]  [MGI Ref ID J:122937]

Seabrook GR; Smith DW; Bowery BJ; Easter A; Reynolds T; Fitzjohn SM; Morton RA; Zheng H; Dawson GR; Sirinathsinghji DJ; Davies CH; Collingridge GL; Hill RG. 1999. Mechanisms contributing to the deficits in hippocampal synaptic plasticity in mice lacking amyloid precursor protein. Neuropharmacology 38(3):349-59. [PubMed: 10219973]  [MGI Ref ID J:54292]

Sosa LJ; Bergman J; Estrada-Bernal A; Glorioso TJ; Kittelson JM; Pfenninger KH. 2013. Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance. PLoS One 8(5):e64521. [PubMed: 23691241]  [MGI Ref ID J:204265]

Stokin GB; Lillo C; Falzone TL; Brusch RG; Rockenstein E; Mount SL; Raman R; Davies P; Masliah E; Williams DS; Goldstein LS. 2005. Axonopathy and transport deficits early in the pathogenesis of Alzheimer's disease. Science 307(5713):1282-8. [PubMed: 15731448]  [MGI Ref ID J:96346]

Szpankowski L; Encalada SE; Goldstein LS. 2012. Subpixel colocalization reveals amyloid precursor protein-dependent kinesin-1 and dynein association with axonal vesicles. Proc Natl Acad Sci U S A 109(22):8582-7. [PubMed: 22582169]  [MGI Ref ID J:184761]

Takahashi RH; Milner TA; Li F; Nam EE; Edgar MA; Yamaguchi H; Beal MF; Xu H; Greengard P; Gouras GK. 2002. Intraneuronal Alzheimer abeta42 accumulates in multivesicular bodies and is associated with synaptic pathology. Am J Pathol 161(5):1869-79. [PubMed: 12414533]  [MGI Ref ID J:79908]

Tamboli IY; Prager K; Thal DR; Thelen KM; Dewachter I; Pietrzik CU; St George-Hyslop P; Sisodia SS; De Strooper B; Heneka MT; Filippov MA; Muller U; van Leuven F; Lutjohann D; Walter J. 2008. Loss of gamma-secretase function impairs endocytosis of lipoprotein particles and membrane cholesterol homeostasis. J Neurosci 28(46):12097-106. [PubMed: 19005074]  [MGI Ref ID J:142399]

Thinakaran G; Koo EH. 2008. Amyloid precursor protein trafficking, processing, and function. J Biol Chem 283(44):29615-9. [PubMed: 18650430]  [MGI Ref ID J:142479]

Tu Z; Keller MP; Zhang C; Rabaglia ME; Greenawalt DM; Yang X; Wang IM; Dai H; Bruss MD; Lum PY; Zhou YP; Kemp DM; Kendziorski C; Yandell BS; Attie AD; Schadt EE; Zhu J. 2012. Integrative analysis of a cross-loci regulation network identifies App as a gene regulating insulin secretion from pancreatic islets. PLoS Genet 8(12):e1003107. [PubMed: 23236292]  [MGI Ref ID J:194853]

Tyan SH; Shih AY; Walsh JJ; Maruyama H; Sarsoza F; Ku L; Eggert S; Hof PR; Koo EH; Dickstein DL. 2012. Amyloid precursor protein (APP) regulates synaptic structure and function. Mol Cell Neurosci 51(1-2):43-52. [PubMed: 22884903]  [MGI Ref ID J:203584]

Wang B; Yang L; Wang Z; Zheng H. 2007. Amyolid precursor protein mediates presynaptic localization and activity of the high-affinity choline transporter. Proc Natl Acad Sci U S A 104(35):14140-5. [PubMed: 17709753]  [MGI Ref ID J:143819]

Wang P; Yang G; Mosier DR; Chang P; Zaidi T; Gong YD; Zhao NM; Dominguez B; Lee KF; Gan WB; Zheng H. 2005. Defective neuromuscular synapses in mice lacking amyloid precursor protein (APP) and APP-Like protein 2. J Neurosci 25(5):1219-25. [PubMed: 15689559]  [MGI Ref ID J:98108]

Westmark CJ; Westmark PR; O'Riordan KJ; Ray BC; Hervey CM; Salamat MS; Abozeid SH; Stein KM; Stodola LA; Tranfaglia M; Burger C; Berry-Kravis EM; Malter JS. 2011. Reversal of Fragile X Phenotypes by Manipulation of AbetaPP/Abeta Levels in Fmr1 Mice. PLoS One 6(10):e26549. [PubMed: 22046307]  [MGI Ref ID J:178076]

White AR; Multhaup G; Maher F; Bellingham S; Camakaris J; Zheng H; Bush AI; Beyreuther K; Masters CL; Cappai R. 1999. The Alzheimer's disease amyloid precursor protein modulates copper-induced toxicity and oxidative stress in primary neuronal cultures. J Neurosci 19(21):9170-9. [PubMed: 10531420]  [MGI Ref ID J:119903]

White AR; Reyes R; Mercer JF; Camakaris J; Zheng H; Bush AI; Multhaup G; Beyreuther K; Masters CL; Cappai R. 1999. Copper levels are increased in the cerebral cortex and liver of APP and APLP2 knockout mice. Brain Res 842(2):439-44. [PubMed: 10526140]  [MGI Ref ID J:58021]

Xu F; Davis J; Miao J; Previti ML; Romanov G; Ziegler K; Van Nostrand WE. 2005. Protease nexin-2/amyloid beta-protein precursor limits cerebral thrombosis. Proc Natl Acad Sci U S A 102(50):18135-40. [PubMed: 16330760]  [MGI Ref ID J:104364]

Xu F; Previti ML; Nieman MT; Davis J; Schmaier AH; Van Nostrand WE. 2009. AbetaPP/APLP2 family of Kunitz serine proteinase inhibitors regulate cerebral thrombosis. J Neurosci 29(17):5666-70. [PubMed: 19403832]  [MGI Ref ID J:155652]

Yang G; Gong YD; Gong K; Jiang WL; Kwon E; Wang P; Zheng H; Zhang XF; Gan WB; Zhao NM. 2005. Reduced synaptic vesicle density and active zone size in mice lacking amyloid precursor protein (APP) and APP-like protein 2. Neurosci Lett 384(1-2):66-71. [PubMed: 15919150]  [MGI Ref ID J:100994]

Yang L; Wang B; Long C; Wu G; Zheng H. 2007. Increased asynchronous release and aberrant calcium channel activation in amyloid precursor protein deficient neuromuscular synapses. Neuroscience 149(4):768-78. [PubMed: 17919826]  [MGI Ref ID J:141485]

Yang L; Wang Z; Wang B; Justice NJ; Zheng H. 2009. Amyloid precursor protein regulates Cav1.2 L-type calcium channel levels and function to influence GABAergic short-term plasticity. J Neurosci 29(50):15660-8. [PubMed: 20016080]  [MGI Ref ID J:157106]

Zhang W; Thevapriya S; Kim PJ; Yu WP; Shawn Je H; King Tan E; Zeng L. 2014. Amyloid precursor protein regulates neurogenesis by antagonizing miR-574-5p in the developing cerebral cortex. Nat Commun 5:3330. [PubMed: 24584353]  [MGI Ref ID J:210257]

Zhang YW; Wang R; Liu Q; Zhang H; Liao FF; Xu H. 2007. Presenilin/{gamma}-secretase-dependent processing of beta-amyloid precursor protein regulates EGF receptor expression. Proc Natl Acad Sci U S A 104(25):10613-8. [PubMed: 17556541]  [MGI Ref ID J:122380]

von Koch CS; Zheng H; Chen H; Trumbauer M; Thinakaran G; van der Ploeg LH; Price DL; Sisodia SS. 1997. Generation of APLP2 KO mice and early postnatal lethality in APLP2/APP double KO mice. Neurobiol Aging 18(6):661-9. [PubMed: 9461064]  [MGI Ref ID J:45851]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129S7 background and has been backcrossed to C57BL/6J for at least ten generations (7/01). Coat color expected from breeding:Black
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Apptm1Dbo  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Apptm1Dbo x Homozygous for Apptm1Dbo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Apptm1Dbo  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Apptm1Dbo x Homozygous for Apptm1Dbo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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