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Genes & Alleles

Aplp2;   Aplp2tm1Dbo;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator David Borchelt,   McKnight Brain Inst, Univ of Florida

Strain Description
Mice that are homozygous null for the Aplp2 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Aplp2 gene product (mRNA or protein) is detected. This strain may be useful in studies related to Alzheimer's Disease, especially if used in conjunction with other mutant mouse strains (see B6.129S7-App^tm1Dbo, Stock No. 004133).

Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Aplp2 gene encoding the promoter and exon 1. The construct was electroporated into 129S7/SvEvBrd-derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Aplp2tm1Dbo/Aplp2tm1Dbo         involves: 129S7/SvEvBrd * C57BL/6J normal phenotype no abnormal phenotype detected (MGI Ref ID J:45851) normal size, fertility and behavior

Gene & Allele Details

Allele Symbol		Aplp2tm1Dbo
Allele Name		targeted mutation 1, David R Borchelt
Mutation Made By		David Borchelt,   McKnight Brain Inst, Univ of Florida
Strain of Origin		129S7/SvEvBrd-Hprt1
ES Cell Line Name		AB2.1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1
Gene Symbol and Name		Aplp2, amyloid beta (A4) precursor-like protein 2
Chromosome		9
Gene Common Name(s)		AI790698; APPH; APPL2; CDEBP; WALPLP2; expressed sequence AI790698;
Molecular Note		Replacement of the promoter region and first exon with a neomycin cassette. Western blot analysis on samples derived from nervous tissue and other peripheral tissues of homozygous mutant mice failed to detect any protein. [MGI Ref ID J:45851]

Control Information

Allele	Control
Aplp2tm1Dbo	Wild-type from the colony
Considerations for Choosing Controls

Genotyping Protocols

Aplp2^tm1Dbo

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129S7 background and has been backcrossed to C57BL/6J for at least 5 generations (7/01).

Additional Web Information

Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Research Applications

This mouse can be used to support research in many areas including:

Aplp2^tm1Dbo related

Mouse/Human Gene Homologs
Alzheimer's

Neurobiology Research
Alzheimer's Disease
Neurodegeneration

References

Selected Reference(s)

von Koch CS; Zheng H; Chen H; Trumbauer M; Thinakaran G; van der Ploeg LH; Price DL; Sisodia SS. 1997. Generation of APLP2 KO mice and early postnatal lethality in APLP2/APP double KO mice. Neurobiol Aging 18(6):661-9. [PubMed: 9461064]  [MGI Ref ID J:45851]

Additional References

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Price and Supply Information

Strain Name:	B6.129S7-Aplp2tm1Dbo/J
Stock Number:	004142

Price Details

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Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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