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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Dr. David R. Borchelt, McKnight Brain Inst, Univ of Florida Description
Mice that are homozygous null for the Aplp2 gene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Aplp2 gene product (mRNA or protein) is detected. This strain may be useful in studies related to Alzheimer's Disease, especially if used in conjunction with other mutant mouse strains (see B6.129S7-Apptm1Dbo, Stock No. 004133).Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Aplp2 gene encoding the promoter and exon 1. The construct was electroporated into 129S7/SvEvBrd-derived AB2.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (107 strains)
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Aplp2tm1Dbo/Aplp2tm1Dbo
involves: 129S7/SvEvBrd * C57BL/6J
- normal phenotype
- no abnormal phenotype detected
- normal size, fertility and behavior (MGI Ref ID J:45851)
This mouse can be used to support research in many areas including:Aplp2tm1Dbo related
Neurobiology Research
Alzheimer's Disease
Neurodegeneration
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Aplp2tm1Dbo | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, David R Borchelt | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | APLP2-; | ||
| Mutation Made By | Dr. David Borchelt, McKnight Brain Inst, Univ of Florida | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt | ||
| ES Cell Line Name | AB2.1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt | ||
| Gene Symbol and Name | Aplp2, amyloid beta (A4) precursor-like protein 2 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | AI790698; APLP-2; APPH; APPL2; CDEBP; expressed sequence AI790698; | ||
| Molecular Note | Replacement of the promoter region and first exon with a neomycin cassette. Western blot analysis on samples derived from nervous tissue and other peripheral tissues of homozygous mutant mice failed to detect any protein. [MGI Ref ID J:45851] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Aplp2tm1Dbo, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
von Koch CS; Zheng H; Chen H; Trumbauer M; Thinakaran G; van der Ploeg LH; Price DL; Sisodia SS. 1997. Generation of APLP2 KO mice and early postnatal lethality in APLP2/APP double KO mice. Neurobiol Aging 18(6):661-9. [PubMed: 9461064] [MGI Ref ID J:45851]
Additional References
Aplp2tm1Dbo relatedAydin D; Filippov MA; Tschape JA; Gretz N; Prinz M; Eils R; Brors B; Muller UC. 2011. Comparative transcriptome profiling of Amyloid Precursor Protein family members in the adult cortex. BMC Genomics 12(1):160. [PubMed: 21435241] [MGI Ref ID J:170309]
Barbagallo AP; Wang Z; Zheng H; D'Adamio L. 2011. A single tyrosine residue in the amyloid precursor protein intracellular domain is essential for developmental function. J Biol Chem 286(11):8717-21. [PubMed: 21266574] [MGI Ref ID J:170948]
Barbagallo AP; Wang Z; Zheng H; D'Adamio L. 2011. The intracellular threonine of amyloid precursor protein that is essential for docking of Pin1 is dispensable for developmental function. PLoS One 6(3):e18006. [PubMed: 21445342] [MGI Ref ID J:171673]
Bellingham SA; Ciccotosto GD; Needham BE; Fodero LR; White AR; Masters CL; Cappai R; Camakaris J. 2004. Gene knockout of amyloid precursor protein and amyloid precursor-like protein-2 increases cellular copper levels in primary mouse cortical neurons and embryonic fibroblasts. J Neurochem 91(2):423-8. [PubMed: 15447675] [MGI Ref ID J:93279]
Cappai R; Cheng F; Ciccotosto GD; Needham BE; Masters CL; Multhaup G; Fransson LA; Mani K. 2005. The amyloid precursor protein (APP) of Alzheimer disease and its paralog, APLP2, modulate the Cu/Zn-Nitric Oxide-catalyzed degradation of glypican-1 heparan sulfate in vivo. J Biol Chem 280(14):13913-20. [PubMed: 15677459] [MGI Ref ID J:98747]
Heber S; Herms J; Gajic V; Hainfellner J; Aguzzi A; Rulicke T; Kretzschmar H; von Koch C; Sisodia S; Tremml P; Lipp HP; Wolfer DP; Muller U. 2000. Mice with combined gene knock-outs reveal essential and partially redundant functions of amyloid precursor protein family members J Neurosci 20(21):7951-63. [PubMed: 11050115] [MGI Ref ID J:65196]
Herms J; Anliker B; Heber S; Ring S; Fuhrmann M; Kretzschmar H; Sisodia S; Muller U. 2004. Cortical dysplasia resembling human type 2 lissencephaly in mice lacking all three APP family members. EMBO J 23(20):4106-15. [PubMed: 15385965] [MGI Ref ID J:93306]
Laky K; Annaert W; Fowlkes BJ. 2009. Amyloid precursor family proteins are expressed by thymic and lymph node stromal cells but are not required for lymphocyte development. Int Immunol 21(10):1163-74. [PubMed: 19710207] [MGI Ref ID J:153769]
Li H; Wang B; Wang Z; Guo Q; Tabuchi K; Hammer RE; Sudhof TC; Zheng H. 2010. Soluble amyloid precursor protein (APP) regulates transthyretin and Klotho gene expression without rescuing the essential function of APP. Proc Natl Acad Sci U S A 107(40):17362-7. [PubMed: 20855613] [MGI Ref ID J:165417]
Li H; Wang Z; Wang B; Guo Q; Dolios G; Tabuchi K; Hammer RE; Sudhof TC; Wang R; Zheng H. 2010. Genetic dissection of the amyloid precursor protein in developmental function and amyloid pathogenesis. J Biol Chem 285(40):30598-605. [PubMed: 20693289] [MGI Ref ID J:166379]
Liu Q; Zerbinatti CV; Zhang J; Hoe HS; Wang B; Cole SL; Herz J; Muglia L; Bu G. 2007. Amyloid precursor protein regulates brain apolipoprotein E and cholesterol metabolism through lipoprotein receptor LRP1. Neuron 56(1):66-78. [PubMed: 17920016] [MGI Ref ID J:126954]
Midthune B; Tyan SH; Walsh JJ; Sarsoza F; Eggert S; Hof PR; Dickstein DL; Koo EH. 2012. Deletion of the amyloid precursor-like protein 2 (APLP2) does not affect hippocampal neuron morphology or function. Mol Cell Neurosci 49(4):448-55. [PubMed: 22353605] [MGI Ref ID J:196736]
Needham B; Wlodek M; Ciccotosto G; Fam B; Masters C; Proietto J; Andrikopoulos S; Cappai R. 2008. Identification of the Alzheimer's disease amyloid precursor protein (APP) and its homologue APLP2 as essential modulators of glucose and insulin homeostasis and growth. J Pathol 215(2):155-63. [PubMed: 18393365] [MGI Ref ID J:134311]
Pardossi-Piquard R; Petit A; Kawarai T; Sunyach C; Alves da Costa C; Vincent B; Ring S; D'Adamio L; Shen J; Muller U; St George Hyslop P; Checler F. 2005. Presenilin-dependent transcriptional control of the Abeta-degrading enzyme neprilysin by intracellular domains of betaAPP and APLP. Neuron 46(4):541-54. [PubMed: 15944124] [MGI Ref ID J:100260]
Siemes C; Quast T; Kummer C; Wehner S; Kirfel G; Muller U; Herzog V. 2006. Keratinocytes from APP/APLP2-deficient mice are impaired in proliferation, adhesion and migration in vitro. Exp Cell Res 312(11):1939-49. [PubMed: 16584729] [MGI Ref ID J:111366]
Soba P; Eggert S; Wagner K; Zentgraf H; Siehl K; Kreger S; Lower A; Langer A; Merdes G; Paro R; Masters CL; Muller U; Kins S; Beyreuther K. 2005. Homo- and heterodimerization of APP family members promotes intercellular adhesion. EMBO J 24(20):3624-34. [PubMed: 16193067] [MGI Ref ID J:154833]
Tamboli IY; Prager K; Thal DR; Thelen KM; Dewachter I; Pietrzik CU; St George-Hyslop P; Sisodia SS; De Strooper B; Heneka MT; Filippov MA; Muller U; van Leuven F; Lutjohann D; Walter J. 2008. Loss of gamma-secretase function impairs endocytosis of lipoprotein particles and membrane cholesterol homeostasis. J Neurosci 28(46):12097-106. [PubMed: 19005074] [MGI Ref ID J:142399]
Thinakaran G; Koo EH. 2008. Amyloid precursor protein trafficking, processing, and function. J Biol Chem 283(44):29615-9. [PubMed: 18650430] [MGI Ref ID J:142479]
Wang B; Yang L; Wang Z; Zheng H. 2007. Amyolid precursor protein mediates presynaptic localization and activity of the high-affinity choline transporter. Proc Natl Acad Sci U S A 104(35):14140-5. [PubMed: 17709753] [MGI Ref ID J:143819]
Wang P; Yang G; Mosier DR; Chang P; Zaidi T; Gong YD; Zhao NM; Dominguez B; Lee KF; Gan WB; Zheng H. 2005. Defective neuromuscular synapses in mice lacking amyloid precursor protein (APP) and APP-Like protein 2. J Neurosci 25(5):1219-25. [PubMed: 15689559] [MGI Ref ID J:98108]
Wang Z; Wang B; Yang L; Guo Q; Aithmitti N; Songyang Z; Zheng H. 2009. Presynaptic and postsynaptic interaction of the amyloid precursor protein promotes peripheral and central synaptogenesis. J Neurosci 29(35):10788-801. [PubMed: 19726636] [MGI Ref ID J:152457]
Weyer SW; Klevanski M; Delekate A; Voikar V; Aydin D; Hick M; Filippov M; Drost N; Schaller KL; Saar M; Vogt MA; Gass P; Samanta A; Jaschke A; Korte M; Wolfer DP; Caldwell JH; Muller UC. 2011. APP and APLP2 are essential at PNS and CNS synapses for transmission, spatial learning and LTP. EMBO J 30(11):2266-80. [PubMed: 21522131] [MGI Ref ID J:173191]
White AR; Reyes R; Mercer JF; Camakaris J; Zheng H; Bush AI; Multhaup G; Beyreuther K; Masters CL; Cappai R. 1999. Copper levels are increased in the cerebral cortex and liver of APP and APLP2 knockout mice. Brain Res 842(2):439-44. [PubMed: 10526140] [MGI Ref ID J:58021]
Xu F; Previti ML; Nieman MT; Davis J; Schmaier AH; Van Nostrand WE. 2009. AbetaPP/APLP2 family of Kunitz serine proteinase inhibitors regulate cerebral thrombosis. J Neurosci 29(17):5666-70. [PubMed: 19403832] [MGI Ref ID J:155652]
Yang G; Gong YD; Gong K; Jiang WL; Kwon E; Wang P; Zheng H; Zhang XF; Gan WB; Zhao NM. 2005. Reduced synaptic vesicle density and active zone size in mice lacking amyloid precursor protein (APP) and APP-like protein 2. Neurosci Lett 384(1-2):66-71. [PubMed: 15919150] [MGI Ref ID J:100994]
Zhang YW; Wang R; Liu Q; Zhang H; Liao FF; Xu H. 2007. Presenilin/{gamma}-secretase-dependent processing of beta-amyloid precursor protein regulates EGF receptor expression. Proc Natl Acad Sci U S A 104(25):10613-8. [PubMed: 17556541] [MGI Ref ID J:122380]
Zheng H; Jiang M; Trumbauer ME; Sirinathsinghji DJ; Hopkins R; Smith DW; Heavens RP; Dawson GR; Boyce S; Conner MW; Stevens KA; Slunt HH; Sisodia SS; Chen HY; Van der Ploeg LHT. 1995. beta-Amyloid precursor protein-deficient mice show reactive gliosis and decreased locomotor activity. Cell 81(4):525-31. [PubMed: 7758106] [MGI Ref ID J:25512]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129S7 background and has been backcrossed to C57BL/6J for at least 5 generations (7/01).
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2085.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2710.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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