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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.129-Tg(Pcp2-Cre)2Mpin (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N4+6F13 (08-JAN-08) Donating Investigator Michael Meyer, Ludwig-Maximilians-University Munich Description
These transgenic mice express a cre gene inserted into exon 4 of a Pcp2 gene. Mice homozygous for the insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is observed in most Purkinje cells and some retinal bipolar neurons. Small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue. Recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Pcp2
004690 B6;FVB-Tg(Pcp2-EGFP)2Yuza/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 006207 STOCK Tg(Pcp2-cre)1Amc/J View Strains carrying other alleles of Pcp2 (3 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Pcp2-cre)2Mpin/0
involves: 129S1/Sv * 129X1/SvJ
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:66884)
- transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cerebellar Defects
Channel and Transporter Defects
Cre-lox System (Cre Recombinase expression in neural tissue)
Neurodegeneration
Research Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Pcp2-cre)2Mpin | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Max-Planck-Institute of Neurobiology | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | L7-cre; L7Cre-2; pcp2Cre-2; | ||
| Mutation Made By | Michael Meyer, Ludwig-Maximilians-University Munich | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | most Purkinje cells and some retinal bipolar neurons; small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue; recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Pcp2, Purkinje cell protein 2 (L7), mouse, laboratory | ||
| Molecular Note | These transgenic mice express Cre recombinase under the direction of the Purkinje cell-specific L7/Pcp2 promoter. Recombinase activity is observed in most Purkinje cells and some retinal bipolar neurons. Small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue. Recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth. [MGI Ref ID J:66884] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR, vers. 1
Generic Cre, STD PCR, vers. 1
Tg(Wnt1-cre)11Rth, Tg(Pcp2-cre)2Mpin, STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Barski JJ; Dethleffsen K; Meyer M. 2000. Cre recombinase expression in cerebellar purkinje cells Genesis 28(3-4):93-8. [PubMed: 11105049] [MGI Ref ID J:66884]
Additional References
Tg(Pcp2-cre)2Mpin relatedAngo F; Wu C; Van der Want JJ; Wu P; Schachner M; Huang ZJ. 2008. Bergmann glia and the recognition molecule CHL1 organize GABAergic axons and direct innervation of Purkinje cell dendrites. PLoS Biol 6(4):e103. [PubMed: 18447583] [MGI Ref ID J:136656]
Barski JJ; Hartmann J; Rose CR; Hoebeek F; Morl K; Noll-Hussong M; De Zeeuw CI; Konnerth A; Meyer M. 2003. Calbindin in cerebellar Purkinje cells is a critical determinant of the precision of motor coordination. J Neurosci 23(8):3469-77. [PubMed: 12716955] [MGI Ref ID J:83378]
Barski JJ; Morl K; Meyer M. 2002. Conditional inactivation of the calbindin D-28k (Calb1) gene by Cre/loxP-mediated recombination. Genesis 32(2):165-8. [PubMed: 11857810] [MGI Ref ID J:75111]
Chen H; McCaffery JM; Chan DC. 2007. Mitochondrial fusion protects against neurodegeneration in the cerebellum. Cell 130(3):548-62. [PubMed: 17693261] [MGI Ref ID J:132329]
Feil R; Hartmann J; Luo C; Wolfsgruber W; Schilling K; Feil S; Barski JJ; Meyer M; Konnerth A; De Zeeuw CI; Hofmann F. 2003. Impairment of LTD and cerebellar learning by Purkinje cell-specific ablation of cGMP-dependent protein kinase I. J Cell Biol 163(2):295-302. [PubMed: 14568994] [MGI Ref ID J:86329]
Koekkoek SK; Yamaguchi K; Milojkovic BA; Dortland BR; Ruigrok TJ; Maex R; De Graaf W; Smit AE; VanderWerf F; Bakker CE; Willemsen R; Ikeda T; Kakizawa S; Onodera K; Nelson DL; Mientjes E; Joosten M; De Schutter E; Oostra BA; Ito M; De Zeeuw CI. 2005. Deletion of FMR1 in Purkinje cells enhances parallel fiber LTD, enlarges spines, and attenuates cerebellar eyelid conditioning in Fragile X syndrome. Neuron 47(3):339-52. [PubMed: 16055059] [MGI Ref ID J:101021]
Komatsu M; Wang QJ; Holstein GR; Friedrich VL Jr; Iwata J; Kominami E; Chait BT; Tanaka K; Yue Z. 2007. Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc Natl Acad Sci U S A 104(36):14489-94. [PubMed: 17726112] [MGI Ref ID J:124947]
Levin SI; Khaliq ZM; Aman TK; Grieco TM; Kearney JA; Raman IM; Meisler MH. 2006. Impaired motor function in mice with cell-specific knockout of sodium channel Scn8a (NaV1.6) in cerebellar purkinje neurons and granule cells. J Neurophysiol 96(2):785-93. [PubMed: 16687615] [MGI Ref ID J:124725]
Matsuda S; Miura E; Matsuda K; Kakegawa W; Kohda K; Watanabe M; Yuzaki M. 2008. Accumulation of AMPA receptors in autophagosomes in neuronal axons lacking adaptor protein AP-4. Neuron 57(5):730-45. [PubMed: 18341993] [MGI Ref ID J:135974]
Mientjes EJ; Nieuwenhuizen I; Kirkpatrick L; Zu T; Hoogeveen-Westerveld M; Severijnen L; Rife M; Willemsen R; Nelson DL; Oostra BA. 2006. The generation of a conditional Fmr1 knock out mouse model to study Fmrp function in vivo. Neurobiol Dis 21(3):549-55. [PubMed: 16257225] [MGI Ref ID J:108008]
Wulff P; Goetz T; Leppa E; Linden AM; Renzi M; Swinny JD; Vekovischeva OY; Sieghart W; Somogyi P; Korpi ER; Farrant M; Wisden W. 2007. From synapse to behavior: rapid modulation of defined neuronal types with engineered GABAA receptors. Nat Neurosci 10(7):923-9. [PubMed: 17572671] [MGI Ref ID J:124171]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129 background and has been crossed to C57BL/6Crl mice at least four times. (7/01) Coat color expeceted from breeding:Black Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $104.80 Female or Male Homozygous for Tg(Pcp2-cre)2Mpin *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $209.60 Homozygous for Tg(Pcp2-cre)2Mpin x Homozygous for Tg(Pcp2-cre)2Mpin
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $136.30 Female or Male Homozygous for Tg(Pcp2-cre)2Mpin *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $272.50 Homozygous for Tg(Pcp2-cre)2Mpin x Homozygous for Tg(Pcp2-cre)2Mpin
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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