Strain Name:

B6.129-Tg(Pcp2-cre)2Mpin/J

Stock Number:

004146

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Former Names B6.129-Tg(Pcp2-Cre)2Mpin    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
GenerationN4+6F13 (08-JAN-08)
 
Donating Investigator Michael Meyer,   Ludwig-Maximilians-University Munich

Description
These transgenic mice express a cre gene inserted into exon 4 of a Pcp2 gene. Mice homozygous for the insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Recombinase activity is observed in most Purkinje cells and some retinal bipolar neurons. Small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue. Recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pcp2
004690   B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
005625   FVB-Tg(Pcp2-tTA)3Horr/J
006207   STOCK Tg(Pcp2-cre)1Amc/J
View Strains carrying other alleles of Pcp2     (3 strains)

Strains carrying other alleles of cre
004337   129(Cg)-Foxg1tm1(cre)Skm/J
008569   129-Alpltm1(cre)Nagy/J
003328   129-Tg(Prm-cre)58Og/J
005989   129;FVB-Tg(PTH-cre)4167Slib/J
007179   129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J
007915   129S.FVB-Tg(Amh-cre)8815Reb/J
004302   129S1-Hprt1tm1(cre)Mnn/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
005697   B6.129-Otx1tm4(cre)Asim/J
006785   B6.129P2(C)-Cd19tm1(cre)Cgn/J
006084   B6.129P2(Cg)-Foxg1tm1(cre)Skm/J
004781   B6.129P2-Lyz2tm1(cre)Ifo/J
005623   B6.129S-Shhtm2(cre/ESR1)Cjt/J
006600   B6.129S1-Mnx1tm4(cre)Tmj/J
005628   B6.129S2-Emx1tm1(cre)Krj/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
006878   B6.129S6-Taglntm2(cre)Yec/J
006054   B6.C-Tg(CMV-cre)1Cgn/J
006230   B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
006149   B6.Cg-Tg(ACTA1-cre)79Jme/J
003574   B6.Cg-Tg(Alb-cre)21Mgn/J
006881   B6.Cg-Tg(Aqp2-cre)1Dek/J
004682   B6.Cg-Tg(CAG-cre/Esr1)5Amc/J
005359   B6.Cg-Tg(Camk2a-cre)T29-1Stl/J
006137   B6.Cg-Tg(Cdh5-cre)7Mlia/J
006368   B6.Cg-Tg(Cr2-cre)3Cgn/J
006663   B6.Cg-Tg(Eno2-cre)39Jme/J
005069   B6.Cg-Tg(Fabp4-cre)1Rev/J
003573   B6.Cg-Tg(Ins2-cre)25Mgn/J
008068   B6.Cg-Tg(Itgax-cre)1-1Reiz/J
003802   B6.Cg-Tg(Lck-cre)548Jxm/J
003556   B6.Cg-Tg(Mx1-cre)1Cgn/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
005657   B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J
003771   B6.Cg-Tg(Nes-cre)1Kln/J
005975   B6.Cg-Tg(Plp1-cre/ESR1)3.16Pop/J
005584   B6.Cg-Tg(Prrx1-cre)1Cjt/J
003967   B6.Cg-Tg(Rbp3-cre)528Jxm/J
008454   B6.Cg-Tg(Sox2-cre)1Amc/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
003966   B6.Cg-Tg(Syn1-cre)671Jxm/J
004128   B6.Cg-Tg(Tek-cre)12Flv/J
007606   B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J
008085   B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
006475   B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J
006451   B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J
006333   B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J
003724   B6.FVB-Tg(EIIa-cre)C5379Lmgd/J
006660   B6.SJL-Slc6a3tm1.1(cre)Bkmn/J
004586   B6.SJL-Tg(Vil-cre)997Gum/J
005650   B6129-Tg(Myh6-cre/Esr1)1Jmk/J
003552   B6129-Tg(Wap-cre)11738Mam/J
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
005549   B6;129-Pax3tm1(cre)Joe/J
008529   B6;129P-Tg(Neurog1-cre/ESR1)1Good/J
006668   B6;129P2-Omptm4(cre)Mom/MomJ
007001   B6;129S-Tg(UBC-cre/ESR1)1Ejb/J
006410   B6;129S6-Chattm1(cre)Lowl/J
003466   B6;D2-Tg(Sycp1-cre)4Min/J
008533   B6;FVB-Tg(Cspg4-cre)1Akik/J
003734   B6;FVB-Tg(GZMB-cre)1Jcb/J
006302   B6;SJL-Slc6a3tm1.1(cre)Bkmn/J
004426   B6;SJL-Tg(Cga-cre)3Sac/J
003554   B6;SJL-Tg(Col2a1-cre)1Bhr/J
005249   B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
007610   B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J
007252   B6Ei.129S4-Tg(Prm-cre)58Og/EiJ
003465   BALB/c-Tg(CMV-cre)1Cgn/J
004126   C.Cg-Cd19tm1(cre)Cgn Ighb/J
005673   C.Cg-Tg(Mx1-cre)1Cgn/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
008535   C57BL/6-Tg(Cxcl4-cre)Q3Rsko/J
006474   C57BL/6-Tg(Grik4-cre)G32-4Stl/J
008314   C57BL/6-Tg(HBB-cre)12Kpe/J
006888   C57BL/6-Tg(Zp3-cre)1Gwh/J
003394   C57BL/6-Tg(Zp3-cre)3Mrt/J
003651   C57BL/6-Tg(Zp3-cre)93Knw/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
008661   C57BL/6J-Tg(Nkx2-1-cre)2Sand/J
006405   FVB-Tg(Ckmm-cre)5Khn/J
006774   FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J
006954   FVB-Tg(Ddx4-cre)1Dcas/J
004600   FVB-Tg(GFAP-cre)25Mes/J
006364   FVB-Tg(Nr5a1-cre)2Lowl/J
006139   FVB.Cg-Tg(ACTA1-cre)79Jme/J
006297   FVB.Cg-Tg(Eno2-cre)39Jme/J
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
003376   FVB/N-Tg(ACTB-cre)2Mrt/J
003314   FVB/N-Tg(EIIa-cre)C5379Lmgd/J
006143   FVB/N-Tg(Thy1-cre)1Vln/J
003377   FVB/N-Tg(Zp3-cre)3Mrt/J
005732   NOD.Cg-Tg(Lck-cre)548Jxm/AchJ
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
004986   NOD/ShiLt-Tg(Ins2-cre)3Lt/Lt
003855   NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ
004987   NOD/ShiLt-Tg(Ins2-cre)6Lt/Lt
008464   STOCK Foxa2tm2.1(cre/Esr1)Moon/J
004192   STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J
006677   STOCK Olfr151tm28Mom/MomJ
005936   STOCK Tg(ACTA1-cre)79Jme/J
007684   STOCK Tg(Atoh1-cre/ESR1)14Fsh/J
004453   STOCK Tg(CAG-cre/Esr1)5Amc/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005938   STOCK Tg(Eno2-cre)39Jme/J
004692   STOCK Tg(Hoxb7-cre)13Amc/J
008122   STOCK Tg(Ins2-cre/Esr1)1Dam/J
004782   STOCK Tg(KRT14-cre)1Amc/J
005107   STOCK Tg(KRT14-cre/Esr1)20Efu/J
003551   STOCK Tg(MMTV-cre)1Mam/J
003553   STOCK Tg(MMTV-cre)4Mam/J
002527   STOCK Tg(Mx1-cre)1Cgn/J
002858   STOCK Tg(Nes-cre)1Wme/J
002859   STOCK Tg(Nes-cre)2Wme/J
005667   STOCK Tg(Neurog3-cre)C1Able/J
008119   STOCK Tg(Neurog3-cre/Esr1)1Dam/J
006207   STOCK Tg(Pcp2-cre)1Amc/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
006395   STOCK Tg(Sim1-cre)1Lowl/J
004783   STOCK Tg(Sox2-cre)1Amc/J
004746   STOCK Tg(Tagln-cre)1Her/J
003829   STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
002471   STOCK Tg(hCMV-cre)140Sau/J
006224   STOCK Tg(tetO-cre)1Jaw/J
View Strains carrying other alleles of cre     (125 strains)

Additional Web Information

Congenic Nomenclature
Cre-lox Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(Pcp2-cre)2Mpin/0

        involves: 129S1/Sv * 129X1/SvJ
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:66884)
    • transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cerebellar Defects
Channel and Transporter Defects
Cre-lox System (Cre Recombinase expression in neural tissue)
Neurodegeneration

Research Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Neurobiology Research

cre related

Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Pcp2-cre)2Mpin
Allele Name transgene insertion 2, Max-Planck-Institute of Neurobiology
Allele Type Transgenic (Cre/Flp)
Common Name(s) L7-cre; L7Cre-2; pcp2Cre-2;
Mutation Made By Michael Meyer,   Ludwig-Maximilians-University Munich
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Site of Expressionmost Purkinje cells and some retinal bipolar neurons; small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue; recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth
Expressed Gene cre, cre recombinase, bacteriophage P1
Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence.
Promoter Pcp2, Purkinje cell protein 2 (L7), mouse, laboratory
Molecular Note These transgenic mice express Cre recombinase under the direction of the Purkinje cell-specific L7/Pcp2 promoter. Recombinase activity is observed in most Purkinje cells and some retinal bipolar neurons. Small amounts of activity are observed in an unidentified population of cells of the central nervous system tissue. Recombination is first observed around postnatal day 6 and is fully established 2 to 3 weeks after birth. [MGI Ref ID J:66884]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Quantitative PCR, QPCR, vers. 1
Generic Cre, STD PCR, vers. 1
Tg(Wnt1-cre)11Rth, Tg(Pcp2-cre)2Mpin, STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Barski JJ; Dethleffsen K; Meyer M. 2000. Cre recombinase expression in cerebellar purkinje cells Genesis 28(3-4):93-8. [PubMed: 11105049]  [MGI Ref ID J:66884]

Additional References

Tg(Pcp2-cre)2Mpin related

Ango F; Wu C; Van der Want JJ; Wu P; Schachner M; Huang ZJ. 2008. Bergmann glia and the recognition molecule CHL1 organize GABAergic axons and direct innervation of Purkinje cell dendrites. PLoS Biol 6(4):e103. [PubMed: 18447583]  [MGI Ref ID J:136656]

Barski JJ; Hartmann J; Rose CR; Hoebeek F; Morl K; Noll-Hussong M; De Zeeuw CI; Konnerth A; Meyer M. 2003. Calbindin in cerebellar Purkinje cells is a critical determinant of the precision of motor coordination. J Neurosci 23(8):3469-77. [PubMed: 12716955]  [MGI Ref ID J:83378]

Barski JJ; Morl K; Meyer M. 2002. Conditional inactivation of the calbindin D-28k (Calb1) gene by Cre/loxP-mediated recombination. Genesis 32(2):165-8. [PubMed: 11857810]  [MGI Ref ID J:75111]

Chen H; McCaffery JM; Chan DC. 2007. Mitochondrial fusion protects against neurodegeneration in the cerebellum. Cell 130(3):548-62. [PubMed: 17693261]  [MGI Ref ID J:132329]

Feil R; Hartmann J; Luo C; Wolfsgruber W; Schilling K; Feil S; Barski JJ; Meyer M; Konnerth A; De Zeeuw CI; Hofmann F. 2003. Impairment of LTD and cerebellar learning by Purkinje cell-specific ablation of cGMP-dependent protein kinase I. J Cell Biol 163(2):295-302. [PubMed: 14568994]  [MGI Ref ID J:86329]

Koekkoek SK; Yamaguchi K; Milojkovic BA; Dortland BR; Ruigrok TJ; Maex R; De Graaf W; Smit AE; VanderWerf F; Bakker CE; Willemsen R; Ikeda T; Kakizawa S; Onodera K; Nelson DL; Mientjes E; Joosten M; De Schutter E; Oostra BA; Ito M; De Zeeuw CI. 2005. Deletion of FMR1 in Purkinje cells enhances parallel fiber LTD, enlarges spines, and attenuates cerebellar eyelid conditioning in Fragile X syndrome. Neuron 47(3):339-52. [PubMed: 16055059]  [MGI Ref ID J:101021]

Komatsu M; Wang QJ; Holstein GR; Friedrich VL Jr; Iwata J; Kominami E; Chait BT; Tanaka K; Yue Z. 2007. Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc Natl Acad Sci U S A 104(36):14489-94. [PubMed: 17726112]  [MGI Ref ID J:124947]

Levin SI; Khaliq ZM; Aman TK; Grieco TM; Kearney JA; Raman IM; Meisler MH. 2006. Impaired motor function in mice with cell-specific knockout of sodium channel Scn8a (NaV1.6) in cerebellar purkinje neurons and granule cells. J Neurophysiol 96(2):785-93. [PubMed: 16687615]  [MGI Ref ID J:124725]

Matsuda S; Miura E; Matsuda K; Kakegawa W; Kohda K; Watanabe M; Yuzaki M. 2008. Accumulation of AMPA receptors in autophagosomes in neuronal axons lacking adaptor protein AP-4. Neuron 57(5):730-45. [PubMed: 18341993]  [MGI Ref ID J:135974]

Mientjes EJ; Nieuwenhuizen I; Kirkpatrick L; Zu T; Hoogeveen-Westerveld M; Severijnen L; Rife M; Willemsen R; Nelson DL; Oostra BA. 2006. The generation of a conditional Fmr1 knock out mouse model to study Fmrp function in vivo. Neurobiol Dis 21(3):549-55. [PubMed: 16257225]  [MGI Ref ID J:108008]

Wulff P; Goetz T; Leppa E; Linden AM; Renzi M; Swinny JD; Vekovischeva OY; Sieghart W; Somogyi P; Korpi ER; Farrant M; Wisden W. 2007. From synapse to behavior: rapid modulation of defined neuronal types with engineered GABAA receptors. Nat Neurosci 10(7):923-9. [PubMed: 17572671]  [MGI Ref ID J:124171]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129 background and has been crossed to C57BL/6Crl mice at least four times. (7/01) Coat color expeceted from breeding:Black
Mating SystemHomozygote x Homozygote         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $104.80Female or MaleHomozygous for Tg(Pcp2-cre)2Mpin
Pairs /Price*Pair Genotype
$209.60Homozygous for Tg(Pcp2-cre)2Mpin x Homozygous for Tg(Pcp2-cre)2Mpin
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $136.30Female or MaleHomozygous for Tg(Pcp2-cre)2Mpin
Pairs /Price*Pair Genotype
$272.50Homozygous for Tg(Pcp2-cre)2Mpin x Homozygous for Tg(Pcp2-cre)2Mpin
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.


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