Description

Strain Information

Former Names B6.129-Ctnnb1tm2Kem/J    (Changed: 29-NOV-05 ) B6.129-Catnbtm2Kem/J    (Changed: 20-MAY-05 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N10F?+F12N1F2 (05-DEC-07)   Donating Investigator RolfK Kemler,   Max Planck Institute for Immunobiology

Description
These mice possess loxP sites located in introns 1 and 6 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in chrondocytes (see Stock No. 003554 for example), this mutant mouse strain may be useful in studies of chrondocyte differentiation.

When bred to a strain expressing Cre recombinase in heart(see Stock No. 005650 or 005657 for example), this mutant mouse strain may be useful in studies of cardiovascular disease.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was utilized in the construction of this mutant. Similarly oriented loxP sites were placed upstream of exon 2 and flanking the neomycin resistance/ thymidine kinase genes (located in intron 6). The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exons 2-6 were injected in C57BL/6J blastocysts. Resulting chimeric male animals were backcrossed to wildtype C57BL/6J mice.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature
Cre-lox Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Tg(Col2a1-cre)1Bhr/0

        involves: 129S1/Sv * 129X1/SvJ   (conditional)

• craniofacial phenotype
• abnormal craniofacial bone morphology (MGI Ref ID J:90567)
• domed skull (MGI Ref ID J:90567)
• limbs/digits/tail phenotype
• abnormal skeleton extremities morphology (MGI Ref ID J:90567)
  • endochondral bone elements all shorter
  • short limbs
• skeleton phenotype
• abnormal cartilage morphology (MGI Ref ID J:90567)
• abnormal chondrocyte morphology (MGI Ref ID J:90567)
  • chondrocyte morphology reduced about 34%
• abnormal craniofacial bone morphology (MGI Ref ID J:90567)
• domed skull (MGI Ref ID J:90567)
• abnormal skeleton extremities morphology (MGI Ref ID J:90567)
  • endochondral bone elements all shorter
  • short limbs

Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Tg(Myh6-cre/Esr1)1Jmk/0

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * FVB   (conditional)

• cardiovascular system phenotype
• decreased response of heart to induced stress (MGI Ref ID J:109612)
  • mutants treated with tamoxifen and subjected to thoracic aortic constriction exhibit a reduced hypertrophic response to the pressure overload

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)

Ctnnb1^tm2Kem related

Developmental Biology Research
Defects in Cell Adhesion Molecules

Genes & Alleles

Gene & Allele Information

Allele Symbol		Ctnnb1tm2Kem
Allele Name		targeted mutation 2, Rolf Kemler
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Catnb1tm2Kem; Catnbfx; Catnblox(ex2-6); Catnbtm2Kwem; beta-Catflox; beta-catlof; beta-catenin/loxP(ex2-6); beta-cateninc; beta-cateninf; beta-cateninfl; beta-cateninflox; beta-cateninfloxed; beta-cateninlox; floxed;
Mutation Made By		RolfK Kemler,   Max Planck Institute for Immunobiology
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Ctnnb1, catenin (cadherin associated protein), beta 1
Chromosome		9
Gene Common Name(s)		CTNNB; Catnb; DKFZp686D02253; FLJ25606; FLJ37923; beta catenin; beta-catenin; catenin beta;
Molecular Note		A loxP site was inserted in intron 1 and a loxP-flanked neomycin-TK cassette was inserted downstream in intron 6. The neomycin-TK cassette was removed by transient transfection with a Cre recombinase expression vector in ES cells prior to the production of chimeric mice and left two loxP sites flanking a region of gene sequence from exon 2 through exon 6. [MGI Ref ID J:67966]

Genotyping

Genotyping Information

Genotyping Protocols

Ctnnb1^tm2Kem, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Brault V; Moore R; Kutsch S; Ishibashi M; Rowitch DH; McMahon AP; Sommer L; Boussadia O; Kemler R. 2001. Inactivation of the beta-catenin gene by Wnt1-Cre-mediated deletion results in dramatic brain malformation and failure of craniofacial development. Development 128(8):1253-64. [PubMed: 11262227]  [MGI Ref ID J:67966]

Additional References

Akiyama H; Lyons JP; Mori-Akiyama Y; Yang X; Zhang R; Zhang Z; Deng JM; Taketo MM; Nakamura T; Behringer RR; McCrea PD; de Crombrugghe B. 2004. Interactions between Sox9 and beta-catenin control chondrocyte differentiation. Genes Dev 18(9):1072-87. [PubMed: 15132997]  [MGI Ref ID J:90567]

Campos VE; Du M; Li Y. 2004. Increased seizure susceptibility and cortical malformation in beta-catenin mutant mice. Biochem Biophys Res Commun 320(2):606-14. [PubMed: 15219872]  [MGI Ref ID J:92923]

Ctnnb1^tm2Kem related

Akiyama H; Lyons JP; Mori-Akiyama Y; Yang X; Zhang R; Zhang Z; Deng JM; Taketo MM; Nakamura T; Behringer RR; McCrea PD; de Crombrugghe B. 2004. Interactions between Sox9 and beta-catenin control chondrocyte differentiation. Genes Dev 18(9):1072-87. [PubMed: 15132997]  [MGI Ref ID J:90567]

Apte U; Zeng G; Muller P; Tan X; Micsenyi A; Cieply B; Dai C; Liu Y; Kaestner KH; Monga SP. 2006. Activation of Wnt/beta-catenin pathway during hepatocyte growth factor-induced hepatomegaly in mice. Hepatology 44(4):992-1002. [PubMed: 17006939]  [MGI Ref ID J:115725]

Apte U; Zeng G; Thompson MD; Muller P; Micsenyi A; Cieply B; Kaestner KH; Monga SP. 2007. beta-Catenin is critical for early postnatal liver growth. Am J Physiol Gastrointest Liver Physiol 292(6):G1578-85. [PubMed: 17332475]  [MGI Ref ID J:123695]

Arango NA; Szotek PP; Manganaro TF; Oliva E; Donahoe PK; Teixeira J. 2005. Conditional deletion of beta-catenin in the mesenchyme of the developing mouse uterus results in a switch to adipogenesis in the myometrium. Dev Biol 288(1):276-83. [PubMed: 16256976]  [MGI Ref ID J:103530]

Aulehla A; Wiegraebe W; Baubet V; Wahl MB; Deng C; Taketo M; Lewandoski M; Pourquie O. 2008. A beta-catenin gradient links the clock and wavefront systems in mouse embryo segmentation. Nat Cell Biol 10(2):186-93. [PubMed: 18157121]  [MGI Ref ID J:132361]

Backman M; Machon O; Mygland L; van den Bout CJ; Zhong W; Taketo MM; Krauss S. 2005. Effects of canonical Wnt signaling on dorso-ventral specification of the mouse telencephalon. Dev Biol 279(1):155-68. [PubMed: 15708565]  [MGI Ref ID J:96233]

Barrow JR; Thomas KR; Boussadia-Zahui O; Moore R; Kemler R; Capecchi MR; McMahon AP. 2003. Ectodermal Wnt3/beta-catenin signaling is required for the establishment and maintenance of the apical ectodermal ridge. Genes Dev 17(3):394-409. [PubMed: 12569130]  [MGI Ref ID J:81795]

Borello U; Berarducci B; Murphy P; Bajard L; Buffa V; Piccolo S; Buckingham M; Cossu G. 2006. The Wnt/{beta}-catenin pathway regulates Gli-mediated Myf5 expression during somitogenesis. Development 133(18):3723-32. [PubMed: 16936075]  [MGI Ref ID J:112463]

Bridgewater D; Cox B; Cain J; Lau A; Athaide V; Gill PS; Kuure S; Sainio K; Rosenblum ND. 2008. Canonical WNT/beta-catenin signaling is required for ureteric branching. Dev Biol 317(1):83-94. [PubMed: 18358465]  [MGI Ref ID J:136068]

Brunelli S; Relaix F; Baesso S; Buckingham M; Cossu G. 2007. Beta catenin-independent activation of MyoD in presomitic mesoderm requires PKC and depends on Pax3 transcriptional activity. Dev Biol 304(2):604-14. [PubMed: 17275805]  [MGI Ref ID J:122535]

Campos VE; Du M; Li Y. 2004. Increased seizure susceptibility and cortical malformation in beta-catenin mutant mice. Biochem Biophys Res Commun 320(2):606-14. [PubMed: 15219872]  [MGI Ref ID J:92923]

Cattelino A; Liebner S; Gallini R; Zanetti A; Balconi G; Corsi A; Bianco P; Wolburg H; Moore R; Oreda B; Kemler R; Dejana E. 2003. The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility. J Cell Biol 162(6):1111-22. [PubMed: 12975353]  [MGI Ref ID J:85505]

Chang H; Gao F; Guillou F; Taketo MM; Huff V; Behringer RR. 2008. Wt1 negatively regulates {beta}-catenin signaling during testis development. Development 135(10):1875-85. [PubMed: 18403409]  [MGI Ref ID J:134687]

Chen X; Shevtsov SP; Hsich E; Cui L; Haq S; Aronovitz M; Kerkela R; Molkentin JD; Liao R; Salomon RN; Patten R; Force T. 2006. The beta-catenin/T-cell factor/lymphocyte enhancer factor signaling pathway is required for normal and stress-induced cardiac hypertrophy. Mol Cell Biol 26(12):4462-73. [PubMed: 16738313]  [MGI Ref ID J:109612]

Chen Y; Whetstone HC; Lin AC; Nadesan P; Wei Q; Poon R; Alman BA. 2007. Beta-catenin signaling plays a disparate role in different phases of fracture repair: implications for therapy to improve bone healing. PLoS Med 4(7):e249. [PubMed: 17676991]  [MGI Ref ID J:134161]

Chen Y; Whetstone HC; Youn A; Nadesan P; Chow EC; Lin AC; Alman BA. 2007. Beta-catenin signaling pathway is crucial for bone morphogenetic protein 2 to induce new bone formation. J Biol Chem 282(1):526-33. [PubMed: 17085452]  [MGI Ref ID J:118120]

Cheon SS; Wei Q; Gurung A; Youn A; Bright T; Poon R; Whetstone H; Guha A; Alman BA. 2006. Beta-catenin regulates wound size and mediates the effect of TGF-beta in cutaneous healing. FASEB J 20(6):692-701. [PubMed: 16581977]  [MGI Ref ID J:129713]

Cobas M; Wilson A; Ernst B; Mancini SJ; MacDonald HR; Kemler R; Radtke F. 2004. Beta-catenin is dispensable for hematopoiesis and lymphopoiesis. J Exp Med 199(2):221-9. [PubMed: 14718516]  [MGI Ref ID J:89995]

De Langhe SP; Carraro G; Tefft D; Li C; Xu X; Chai Y; Minoo P; Hajihosseini MK; Drouin J; Kaartinen V; Bellusci S. 2008. Formation and Differentiation of Multiple Mesenchymal Lineages during Lung Development Is Regulated by beta-catenin Signaling. PLoS ONE 3(1):e1516. [PubMed: 18231602]  [MGI Ref ID J:131535]

Dean CH; Miller LA; Smith AN; Dufort D; Lang RA; Niswander LA. 2005. Canonical Wnt signaling negatively regulates branching morphogenesis of the lung and lacrimal gland. Dev Biol 286(1):270-86. [PubMed: 16126193]  [MGI Ref ID J:103528]

Deutscher E; Hung-Chang Yao H. 2007. Essential roles of mesenchyme-derived beta-catenin in mouse Mullerian duct morphogenesis. Dev Biol 307(2):227-36. [PubMed: 17532316]  [MGI Ref ID J:122979]

Dunty WC Jr; Biris KK; Chalamalasetty RB; Taketo MM; Lewandoski M; Yamaguchi TP. 2008. Wnt3a/beta-catenin signaling controls posterior body development by coordinating mesoderm formation and segmentation. Development 135(1):85-94. [PubMed: 18045842]  [MGI Ref ID J:129202]

Fu X; Sun H; Klein WH; Mu X. 2006. Beta-catenin is essential for lamination but not neurogenesis in mouse retinal development. Dev Biol 299(2):424-37. [PubMed: 16959241]  [MGI Ref ID J:115047]

Gao X; Arlotta P; Macklis JD; Chen J. 2007. Conditional knock-out of beta-catenin in postnatal-born dentate gyrus granule neurons results in dendritic malformation. J Neurosci 27(52):14317-25. [PubMed: 18160639]  [MGI Ref ID J:130955]

Glass DA 2nd; Bialek P; Ahn JD; Starbuck M; Patel MS; Clevers H; Taketo MM; Long F; McMahon AP; Lang RA; Karsenty G. 2005. Canonical wnt signaling in differentiated osteoblasts controls osteoclast differentiation. Dev Cell 8(5):751-64. [PubMed: 15866165]  [MGI Ref ID J:98430]

Gould TD; O'Donnell KC; Picchini AM; Dow ER; Chen G; Manji HK. 2008. Generation and behavioral characterization of beta-catenin forebrain-specific conditional knock-out mice. Behav Brain Res 189(1):117-25. [PubMed: 18299155]  [MGI Ref ID J:132769]

Grigoryan T; Wend P; Klaus A; Birchmeier W. 2008. Deciphering the function of canonical Wnt signals in development and disease: conditional loss- and gain-of-function mutations of beta-catenin in mice. Genes Dev 22(17):2308-41. [PubMed: 18765787]  [MGI Ref ID J:138781]

Guo W; Lasky JL; Chang CJ; Mosessian S; Lewis X; Xiao Y; Yeh JE; Chen JY; Iruela-Arispe ML; Varella-Garcia M; Wu H. 2008. Multi-genetic events collaboratively contribute to Pten-null leukaemia stem-cell formation. Nature 453(7194):529-33. [PubMed: 18463637]  [MGI Ref ID J:135172]

Hari L; Brault V; Kleber M; Lee HY; Ille F; Leimeroth R; Paratore C; Suter U; Kemler R; Sommer L. 2002. Lineage-specific requirements of beta-catenin in neural crest development. J Cell Biol 159(5):867-80. [PubMed: 12473692]  [MGI Ref ID J:80757]

Holmen SL; Zylstra CR; Mukherjee A; Sigler RE; Faugere MC; Bouxsein ML; Deng L; Clemens TL; Williams BO. 2005. Essential role of beta-catenin in postnatal bone acquisition. J Biol Chem 280(22):21162-8. [PubMed: 15802266]  [MGI Ref ID J:99904]

Hu H; Hilton MJ; Tu X; Yu K; Ornitz DM; Long F. 2005. Sequential roles of Hedgehog and Wnt signaling in osteoblast development. Development 132(1):49-60. [PubMed: 15576404]  [MGI Ref ID J:94274]

Ireland H; Kemp R; Houghton C; Howard L; Clarke AR; Sansom OJ; Winton DJ. 2004. Inducible Cre-mediated control of gene expression in the murine gastrointestinal tract: effect of loss of beta-catenin. Gastroenterology 126(5):1236-46. [PubMed: 15131783]  [MGI Ref ID J:92092]

Jayasena CS; Ohyama T; Segil N; Groves AK. 2008. Notch signaling augments the canonical Wnt pathway to specify the size of the otic placode. Development 135(13):2251-61. [PubMed: 18495817]  [MGI Ref ID J:137096]

Junghans D; Hack I; Frotscher M; Taylor V; Kemler R. 2005. Beta-catenin-mediated cell-adhesion is vital for embryonic forebrain development. Dev Dyn 233(2):528-39. [PubMed: 15844200]  [MGI Ref ID J:129254]

Kim AC; Reuter AL; Zubair M; Else T; Serecky K; Bingham NC; Lavery GG; Parker KL; Hammer GD. 2008. Targeted disruption of beta-catenin in Sf1-expressing cells impairs development and maintenance of the adrenal cortex. Development 135(15):2593-602. [PubMed: 18599507]  [MGI Ref ID J:138575]

Koch U; Wilson A; Cobas M; Kemler R; Macdonald HR; Radtke F. 2008. Simultaneous loss of beta- and gamma-catenin does not perturb hematopoiesis or lymphopoiesis. Blood 111(1):160-4. [PubMed: 17855627]  [MGI Ref ID J:129952]

Koyama E; Shibukawa Y; Nagayama M; Sugito H; Young B; Yuasa T; Okabe T; Ochiai T; Kamiya N; Rountree RB; Kingsley DM; Iwamoto M; Enomoto-Iwamoto M; Pacifici M. 2008. A distinct cohort of progenitor cells participates in synovial joint and articular cartilage formation during mouse limb skeletogenesis. Dev Biol 316(1):62-73. [PubMed: 18295755]  [MGI Ref ID J:135666]

Kreslova J; Machon O; Ruzickova J; Lachova J; Wawrousek EF; Kemler R; Krauss S; Piatigorsky J; Kozmik Z. 2007. Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function. Genesis 45(4):157-68. [PubMed: 17410548]  [MGI Ref ID J:121794]

Li TF; Chen D; Wu Q; Chen M; Sheu TJ; Schwarz EM; Drissi H; Zuscik M; O'Keefe RJ. 2006. Transforming growth factor-beta stimulates cyclin D1 expression through activation of beta-catenin signaling in chondrocytes. J Biol Chem 281(30):21296-304. [PubMed: 16690606]  [MGI Ref ID J:116442]

Lickert H; Cox B; Wehrle C; Taketo MM; Kemler R; Rossant J. 2005. Dissecting Wnt/{beta}-catenin signaling during gastrulation using RNA interference in mouse embryos. Development 132(11):2599-609. [PubMed: 15857914]  [MGI Ref ID J:98484]

Lickert H; Kutsch S; Kanzler B; Tamai Y; Taketo MM; Kemler R. 2002. Formation of multiple hearts in mice following deletion of beta-catenin in the embryonic endoderm. Dev Cell 3(2):171-81. [PubMed: 12194849]  [MGI Ref ID J:108981]

Liebner S; Cattelino A; Gallini R; Rudini N; Iurlaro M; Piccolo S; Dejana E. 2004. Beta-catenin is required for endothelial-mesenchymal transformation during heart cushion development in the mouse. J Cell Biol 166(3):359-67. [PubMed: 15289495]  [MGI Ref ID J:91830]

Lin C; Yin Y; Long F; Ma L. 2008. Tissue-specific requirements of beta-catenin in external genitalia development. Development 135(16):2815-25. [PubMed: 18635608]  [MGI Ref ID J:139251]

Liu B; Yu HM; Hsu W. 2007. Craniosynostosis caused by Axin2 deficiency is mediated through distinct functions of beta-catenin in proliferation and differentiation. Dev Biol 301(1):298-308. [PubMed: 17113065]  [MGI Ref ID J:117081]

Liu F; Thirumangalathu S; Gallant NM; Yang SH; Stoick-Cooper CL; Reddy ST; Andl T; Taketo MM; Dlugosz AA; Moon RT; Barlow LA; Millar SE. 2007. Wnt-beta-catenin signaling initiates taste papilla development. Nat Genet 39(1):106-12. [PubMed: 17128274]  [MGI Ref ID J:117476]

Machon O; Backman M; Machonova O; Kozmik Z; Vacik T; Andersen L; Krauss S. 2007. A dynamic gradient of Wnt signaling controls initiation of neurogenesis in the mammalian cortex and cellular specification in the hippocampus. Dev Biol 311(1):223-37. [PubMed: 17916349]  [MGI Ref ID J:126323]

Machon O; van den Bout CJ; Backman M; Kemler R; Krauss S. 2003. Role of beta-catenin in the developing cortical and hippocampal neuroepithelium. Neuroscience 122(1):129-43. [PubMed: 14596855]  [MGI Ref ID J:109500]

Marose TD; Merkel CE; McMahon AP; Carroll TJ. 2008. Beta-catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state. Dev Biol 314(1):112-26. [PubMed: 18177851]  [MGI Ref ID J:131012]

Murtaugh LC; Law AC; Dor Y; Melton DA. 2005. Beta-catenin is essential for pancreatic acinar but not islet development. Development 132(21):4663-74. [PubMed: 16192304]  [MGI Ref ID J:102698]

Ohtola J; Myers J; Akhtar-Zaidi B; Zuzindlak D; Sandesara P; Yeh K; Mackem S; Atit R. 2008. {beta}-Catenin has sequential roles in the survival and specification of ventral dermis. Development 135(13):2321-9. [PubMed: 18539925]  [MGI Ref ID J:137352]

Ohyama T; Mohamed OA; Taketo MM; Dufort D; Groves AK. 2006. Wnt signals mediate a fate decision between otic placode and epidermis. Development 133(5):865-75. [PubMed: 16452098]  [MGI Ref ID J:105983]

Olson LE; Tollkuhn J; Scafoglio C; Krones A; Zhang J; Ohgi KA; Wu W; Taketo MM; Kemler R; Grosschedl R; Rose D; Li X; Rosenfeld MG. 2006. Homeodomain-mediated beta-catenin-dependent switching events dictate cell-lineage determination. Cell 125(3):593-605. [PubMed: 16678101]  [MGI Ref ID J:114817]

Park JS; Valerius MT; McMahon AP. 2007. Wnt/{beta}-catenin signaling regulates nephron induction during mouse kidney development. Development 134(13):2533-9. [PubMed: 17537789]  [MGI Ref ID J:122521]

Qu J; Zhou J; Ping Yi X; Dong B; Zheng H; Miller LM; Wang X; Schneider MD; Li F. 2007. Cardiac-specific haploinsufficiency of beta-catenin attenuates cardiac hypertrophy but enhances fetal gene expression in response to aortic constriction. J Mol Cell Cardiol 43(3):319-26. [PubMed: 17673255]  [MGI Ref ID J:125231]

Rhee H; Polak L; Fuchs E. 2006. Lhx2 maintains stem cell character in hair follicles. Science 312(5782):1946-9. [PubMed: 16809539]  [MGI Ref ID J:110119]

Rodda SJ; McMahon AP. 2006. Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors. Development 133(16):3231-44. [PubMed: 16854976]  [MGI Ref ID J:114494]

Schuller U; Rowitch DH. 2007. Beta-catenin function is required for cerebellar morphogenesis. Brain Res 1140:161-9. [PubMed: 16824494]  [MGI Ref ID J:120586]

Smith AN; Miller LA; Song N; Taketo MM; Lang RA. 2005. The duality of beta-catenin function: a requirement in lens morphogenesis and signaling suppression of lens fate in periocular ectoderm. Dev Biol 285(2):477-89. [PubMed: 16102745]  [MGI Ref ID J:101264]

Song N; Schwab KR; Patterson LT; Yamaguchi T; Lin X; Potter SS; Lang RA. 2007. pygopus 2 has a crucial, Wnt pathway-independent function in lens induction. Development 134(10):1873-85. [PubMed: 17428831]  [MGI Ref ID J:121416]

Tan X; Behari J; Cieply B; Michalopoulos GK; Monga SP. 2006. Conditional deletion of beta-catenin reveals its role in liver growth and regeneration. Gastroenterology 131(5):1561-72. [PubMed: 17101329]  [MGI Ref ID J:124952]

Woodhead GJ; Mutch CA; Olson EC; Chenn A. 2006. Cell-autonomous beta-catenin signaling regulates cortical precursor proliferation. J Neurosci 26(48):12620-30. [PubMed: 17135424]  [MGI Ref ID J:116183]

Yin Y; White AC; Huh SH; Hilton MJ; Kanazawa H; Long F; Ornitz DM. 2008. An FGF-WNT gene regulatory network controls lung mesenchyme development. Dev Biol 319(2):426-36. [PubMed: 18533146]  [MGI Ref ID J:137691]

Yu Q; Quinn WJ rd; Salay T; Crowley JE; Cancro MP; Sen JM. 2008. Role of beta-catenin in B cell development and function. J Immunol 181(6):3777-83. [PubMed: 18768830]  [MGI Ref ID J:139115]

Zamora M; Manner J; Ruiz-Lozano P. 2007. Epicardium-derived progenitor cells require beta-catenin for coronary artery formation. Proc Natl Acad Sci U S A 104(46):18109-14. [PubMed: 17989236]  [MGI Ref ID J:127444]

Zhang Y; Goss AM; Cohen ED; Kadzik R; Lepore JJ; Muthukumaraswamy K; Yang J; DeMayo FJ; Whitsett JA; Parmacek MS; Morrisey EE. 2008. A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration. Nat Genet 40(7):862-70. [PubMed: 18536717]  [MGI Ref ID J:138407]

Zhao C; Blum J; Chen A; Kwon HY; Jung SH; Cook JM; Lagoo A; Reya T. 2007. Loss of beta-catenin impairs the renewal of normal and CML stem cells in vivo. Cancer Cell 12(6):528-41. [PubMed: 18068630]  [MGI Ref ID J:130324]

Zhou J; Qu J; Yi XP; Graber K; Huber L; Wang X; Gerdes AM; Li F. 2007. Upregulation of gamma-catenin compensates for the loss of beta-catenin in adult cardiomyocytes. Am J Physiol Heart Circ Physiol 292(1):H270-6. [PubMed: 16936006]  [MGI Ref ID J:119968]

de Vries WN; Evsikov AV; Haac BE; Fancher KS; Holbrook AE; Kemler R; Solter D; Knowles BB. 2004. Maternal beta-catenin and E-cadherin in mouse development. Development 131(18):4435-45. [PubMed: 15306566]  [MGI Ref ID J:96042]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129 background and has been backcrossed to C57BL/6J for at least ten generations before being made homozygous. Coat color expected from breeding:Black
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.