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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Former Names B6;129S-Mtap7tm1Sor/J (Changed: 10-JAN-05 ) Type Gene Trap; Mutant Stock; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N3 Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Description
At birth, mice homozygous for the gene-trapped Mtap7 allele are viable, normal in size and do not display any gross physical or behavioral abnormalities. Although trace amounts of a presumably nonfunctional transcript can be detected in testis tissue, no protein product is immunodetectable. Male homozygotes are sterile. Expression of the reporter gene (B-galactosidase from the Bgeo fusion gene) employed by the gene trap vector indicates that the Mtap7 promoter directs expression in various tissues with highest levels seen in the seminiferous tubules. During the first wave of spermatogenesis at 5 weeks of age, deformed spermatids can be observed. Abnormalities are attributed to aberrant microtubule organization. Microtubule aberrations are also observed in Sertoli cells. Gradual loss of germ cells occurs. At three months of age, the testes of homozygous mutants are less than one-third the size of those of heterozygous littermates.Development
A retroviral genetrap vector (ROSABgeo*) containing a Bgal/neo fusion gene (Bgeo) was used to infect 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. ES cells expressing neo (G418 resistant) were selected. Cells were subjected to an additional round of screening for retinoic acid (RA) responsiveness. RA responsive cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were crossed to C57BL/6 animals. The vector was found to have inserted into the 5' portion of the Mtap7 gene.
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (176 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Mtap7Gt(ROSABetageo)1Sor relatedReproductive Biology Research
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)
Research Tools
lacZ Expression
Reproductive Biology Research (male germ cells)
Reproductive Biology Research (testicular cell marker)
Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)
Research Tools
Cell Biology Research
Reproductive Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Mtap7Gt(ROSABetageo)1Sor | ||
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| Allele Name | gene trap 1, Philippe Soriano | ||
| Allele Type | Gene trapped | ||
| Common Name(s) | E-MAP-115-; Mtap7Gt1Sor; | ||
| Mutation Made By | Philippe Soriano, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | The Mtap7 promoter directs expression of the reporter gene in various tissues with highest levels expressed in seminiferous tubules. | ||
| Gene Symbol and Name | Mtap7, microtubule-associated protein 7 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | E-MAP-115; EMAP115; MAP7; R75000; expressed sequence R75000; | ||
| Molecular Note | A lacZ-neomycin gene trap vector inserted in frame at a site that encodes alanine 23. Northern analysis did not detect wild-type mRNA transcripts in homozygous mutant mice. [MGI Ref ID J:62570] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Mtap7Gt(ROSABetageo)1Sor, SEP PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Komada M; McLean DJ; Griswold MD; Russell LD; Soriano P. 2000. E-MAP-115, encoding a microtubule-associated protein, is a retinoic acid-inducible gene required for spermatogenesis. Genes Dev 14(11):1332-42. [PubMed: 10837026] [MGI Ref ID J:62570]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain originated and is maintained on a B6;129S background. *Homozygous males are sterile.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
See Terms of Use
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