Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   01-MAR-06 Species laboratory mouse Generation N9+1F13N1F6 (29-SEP-08) Generation Definitions   Donating Investigator Colin D. Funk,   Queen's University

Description
Mice homozygous for the Alox5^tm1Fun targeted mutation are viable and fertile. In general, homozygous mutant mice are selectively resistant to inflammatory insults. They are resistant to the lethal effects of platelet activating factor but reaction to endotoxin shock is normal. Phorbol ester induced inflammation is normal but arachidonic acid induced inflammation is reduced.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the Alox5 gene. The construct was electroporated into 129S2/SvPas-derived D3H embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The donating investigator reports that the resulting chimeric male animals were backcrossed to C57BL/6J females (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Alox5^tm1Fun allele

002485	129-Alox5tm1Fun/J
002263	B6;129S2-Alox5tm1Fun/J

View Strains carrying   Alox5^tm1Fun     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Asthma, Susceptibility to   (ALOX5)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Alox5^tm1Fun/Alox5^tm1Fun

        B6.Cg-Alox5^tm1Fun

• immune system phenotype
• *normal* immune system phenotype
  • homozygotes display no differences in infarct size following induction of focal cerebral ischemia (permanent or transient; 60 min) relative to wild-type   (MGI Ref ID J:89268)

Alox5^tm1Fun/Alox5^tm1Fun

        B6.129S2-Alox5^tm1Fun/J

• adipose tissue phenotype
• increased total fat pad weight
  • increased total fat pad weight   (MGI Ref ID J:104825)
• homeostasis/metabolism phenotype
• increased circulating VLDL cholesterol level
  • increased plasma VLDL/LDL cholesterol   (MGI Ref ID J:104825)
• increased circulating leptin level   (MGI Ref ID J:104825)
• skeleton phenotype
• increased bone mineral density
  • increased bone mineral density   (MGI Ref ID J:104825)
• immune system phenotype
• decreased susceptibility to experimental autoimmune uveoretinitis
  • upon adoptive transfer of IRBP1-20-specific T cells from wild-type B6 donors to wild-type and Alox5-deficient recipients, wild-type mice develop severe clinical uveitis, whereas mutant recipients show only mild and transient clinical disease   (MGI Ref ID J:108446)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Alox5^tm1Fun/Alox5^tm1Fun

        involves: 129S2/SvPas * C57BL/6

• cardiovascular system phenotype
• *normal* cardiovascular system phenotype
  • homozygotes display a normal mean arterial pressure and heart rate relative to wild-type   (MGI Ref ID J:21372)
• hematopoietic system phenotype
• *normal* hematopoietic system phenotype
  • analysis of cell-surface markers CD3, CD4 and B220 on splenocytes from 4-wk- and 8-wk-old mice revealed no other abnormalities   (MGI Ref ID J:21372)
  • homozygotes show no changes in total blood cell populations or differential cell counts   (MGI Ref ID J:21372)
  • bone marrow analysis revealed no evidence of abnormal precursor cells of any lineage relative to wild-type   (MGI Ref ID J:21372)
• • small spleen
    • homozygotes show no abnormalities up to 10 months of age under normal physiological conditions; however, their spleen is usually smaller relative to wild-type   (MGI Ref ID J:21372)
• immune system phenotype
• *normal* immune system phenotype
  • homozygotes display a normal reaction to endotoxin-induced shock   (MGI Ref ID J:21372)
  • also, homozygotes exhibit a normal reaction to ear inflammation induced by topical application of phorbol myristyl acetate   (MGI Ref ID J:21372)
  • neutrophil recruitment into the peritoneal cavity by a non-specific inflammatory challenge (glycogen) elicits a comparable (large) influx of polymorphonuclear leukocytes in both wild-type and mutant mice   (MGI Ref ID J:21372)
• • abnormal immune system physiology
    • conscious, unanaesthetized homozygotes show resistance to the lethal effects of platelet-activating factor (PAF): they display the same listless behavior as wild-type for the first 20 min but recover quickly   (MGI Ref ID J:21372)
    • mechanically ventilated, anesthetized homozygotes show no signs of broncho-constriction; however, broncho-constriction is observed after methacholine challenge   (MGI Ref ID J:21372)
  • • decreased inflammatory response
      • homozygotes show a significantly reduced reaction to arachidonic acid-induced ear inflammation; administration of leukotrienes rescues this inflammatory phenotype: inflammation is rapid in onset and maximal swelling is reached between 30-60 min   (MGI Ref ID J:21372)
      • homozygotes display a significantly reduced chemotactic infiltration by polymorphonuclear leukocytes following induction of immune complex peritonitis (reverse passive Arthus reaction)   (MGI Ref ID J:21372)
      • in the thioglycollate-elicited peritonitis model, homozygotes only generate about 50% as much peritoneal leukocytosis as wild-type mice at 4.5 h after i.p. thioglycollate challenge; as expected, leukotriene B4 is absent from the mutant peritoneal fluid   (MGI Ref ID J:75371)
      • the specific arachidonate 5-lipoxygenase inhibitor, zileuton, has no effect on the peripheral white-cell count in mutant mice   (MGI Ref ID J:75371)
  • small spleen
    • homozygotes show no abnormalities up to 10 months of age under normal physiological conditions; however, their spleen is usually smaller relative to wild-type   (MGI Ref ID J:21372)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Alox5^tm1Fun related

Immunology, Inflammation and Autoimmunity Research
Inflammation

Metabolism Research
Enzyme Deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Alox5tm1Fun
Allele Name		targeted mutation 1, Colin Funk
Allele Type		Targeted (knock-out)
Common Name(s)		5-LO KO; 5-LO-; 5-LOX K/O; 5-LOX-; 5LO-; 5LX-;
Mutation Made By		Colin Funk,   Queen's University
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3H
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Alox5, arachidonate 5-lipoxygenase
Chromosome		6
Gene Common Name(s)		5-LO; 5-LOX; 5LO; 5LPG; 5LX; AI850497; LOG5; LOX5A; expressed sequence AI850497;
Molecular Note		Insertion of a neomycin resistance cassette into exon 6 disrupted the Alox5 gene. RT-PCR studies did not detect transcript in resident peritoneal macrophages of homozygous mutant mice. Western blot analysis of resident peritoneal macrophages of homozygous mutant mice did not detect ALOX5. [MGI Ref ID J:21372]

Genotyping

Genotyping Information

Genotyping Protocols

Alox5^tm1Fun, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Chen XS; Sheller JR; Johnson EN; Funk CD. 1994. Role of leukotrienes revealed by targeted disruption of the 5-lipoxygenase gene. Nature 372(6502):179-82. [PubMed: 7969451]  [MGI Ref ID J:21372]

Additional References

Kitagawa K; Matsumoto M; Hori M. 2004. Cerebral ischemia in 5-lipoxygenase knockout mice. Brain Res 1004(1-2):198-202. [PubMed: 15033436]  [MGI Ref ID J:89268]

Alox5^tm1Fun related

Adamek A; Jung S; Dienesch C; Laser M; Ertl G; Bauersachs J; Frantz S. 2007. Role of 5-lipoxygenase in myocardial ischemia-reperfusion injury in mice. Eur J Pharmacol 571(1):51-4. [PubMed: 17586489]  [MGI Ref ID J:136740]

Bafica A; Scanga CA; Serhan C; Machado F; White S; Sher A; Aliberti J. 2005. Host control of Mycobacterium tuberculosis is regulated by 5-lipoxygenase-dependent lipoxin production. J Clin Invest 115(6):1601-1606. [PubMed: 15931391]  [MGI Ref ID J:99199]

Bailie MB; Standiford TJ; Laichalk LL; Coffey MJ; Strieter R; Peters-Golden M. 1996. Leukotriene-deficient mice manifest enhanced lethality from Klebsiella pneumonia in association with decreased alveolar macrophage phagocytic and bactericidal activities. J Immunol 157(12):5221-4. [PubMed: 8955165]  [MGI Ref ID J:110715]

Benjamim CF; Canetti C; Cunha FQ; Kunkel SL; Peters-Golden M. 2005. Opposing and hierarchical roles of leukotrienes in local innate immune versus vascular responses in a model of sepsis. J Immunol 174(3):1616-20. [PubMed: 15661924]  [MGI Ref ID J:96404]

Blaho VA; Zhang Y; Hughes-Hanks JM; Brown CR. 2011. 5-Lipoxygenase-Deficient Mice Infected with Borrelia burgdorferi Develop Persistent Arthritis. J Immunol 186(5):3076-84. [PubMed: 21270404]  [MGI Ref ID J:169390]

Canetti C; Aronoff DM; Choe M; Flamand N; Wettlaufer S; Toews GB; Chen GH; Peters-Golden M. 2006. Differential regulation by leukotrienes and calcium of Fc gamma receptor-induced phagocytosis and Syk activation in dendritic cells versus macrophages. J Leukoc Biol 79(6):1234-41. [PubMed: 16574771]  [MGI Ref ID J:109680]

Cao RY; Adams MA; Habenicht AJ; Funk CD. 2007. Angiotensin II-induced abdominal aortic aneurysm occurs independently of the 5-lipoxygenase pathway in apolipoprotein E-deficient mice. Prostaglandins Other Lipid Mediat 84(1-2):34-42. [PubMed: 17643886]  [MGI Ref ID J:129321]

Chen Y; Hu Y; Zhang H; Peng C; Li S. 2009. Loss of the Alox5 gene impairs leukemia stem cells and prevents chronic myeloid leukemia. Nat Genet 41(7):783-92. [PubMed: 19503090]  [MGI Ref ID J:151419]

Chen Y; Sullivan C; Peng C; Shan Y; Hu Y; Li D; Li S. 2011. A tumor suppressor function of the Msr1 gene in leukemia stem cells of chronic myeloid leukemia. Blood 118(2):390-400. [PubMed: 21596859]  [MGI Ref ID J:174876]

Chen Y; Zhu J; Lum PY; Yang X; Pinto S; MacNeil DJ; Zhang C; Lamb J; Edwards S; Sieberts SK; Leonardson A; Castellini LW; Wang S; Champy MF; Zhang B; Emilsson V; Doss S; Ghazalpour A; Horvath S; Drake TA; Lusis AJ; Schadt EE. 2008. Variations in DNA elucidate molecular networks that cause disease. Nature 452(7186):429-35. [PubMed: 18344982]  [MGI Ref ID J:134171]

Cheon EC; Khazaie K; Khan MW; Strouch MJ; Krantz SB; Phillips J; Blatner NR; Hix LM; Zhang M; Dennis KL; Salabat MR; Heiferman M; Grippo PJ; Munshi HG; Gounaris E; Bentrem DJ. 2011. Mast Cell 5-Lipoxygenase Activity Promotes Intestinal Polyposis in APC{Delta}468 Mice. Cancer Res 71(5):1627-36. [PubMed: 21216893]  [MGI Ref ID J:169312]

Chou VP; Holman TR; Manning-Bog AB. 2013. Differential contribution of lipoxygenase isozymes to nigrostriatal vulnerability. Neuroscience 228:73-82. [PubMed: 23079635]  [MGI Ref ID J:194022]

Collin M; Rossi A; Cuzzocrea S; Patel NS; Di Paola R; Hadley J; Collino M; Sautebin L; Thiemermann C. 2004. Reduction of the multiple organ injury and dysfunction caused by endotoxemia in 5-lipoxygenase knockout mice and by the 5-lipoxygenase inhibitor zileuton. J Leukoc Biol 76(5):961-70. [PubMed: 15328337]  [MGI Ref ID J:93517]

Cuzzocrea S; Rossi A; Serraino I; Di Paola R; Dugo L; Genovese T; Britti D; Sciarra G; De Sarro A; Caputi AP; Sautebin L. 2003. 5-lipoxygenase knockout mice exhibit a resistance to acute pancreatitis induced by cerulein. Immunology 110(1):120-30. [PubMed: 12941149]  [MGI Ref ID J:113639]

Cuzzocrea S; Rossi A; Serraino I; Mazzon E; Di Paola R; Dugo L; Genovese T; Calabro B; Caputi AP; Sautebin L. 2003. 5-Lipoxygenase knockout mice exhibit a resistance to pleurisy and lung injury caused by carrageenan. J Leukoc Biol 73(6):739-46. [PubMed: 12773506]  [MGI Ref ID J:120440]

DiMeo D; Tian J; Zhang J; Narushima S; Berg DJ. 2008. Increased interleukin-10 production and Th2 skewing in the absence of 5-lipoxygenase. Immunology 123(2):250-62. [PubMed: 17894798]  [MGI Ref ID J:134808]

Emerson MR; LeVine SM. 2004. Experimental allergic encephalomyelitis is exacerbated in mice deficient for 12/15-lipoxygenase or 5-lipoxygenase. Brain Res 1021(1):140-5. [PubMed: 15328042]  [MGI Ref ID J:92011]

Esper L; Roman-Campos D; Lara A; Brant F; Castro LL; Barroso A; Araujo RR; Vieira LQ; Mukherjee S; Gomes ER; Rocha NN; Ramos IP; Lisanti MP; Campos CF; Arantes RM; Guatimosim S; Weiss LM; Cruz JS; Tanowitz HB; Teixeira MM; Machado FS. 2012. Role of SOCS2 in Modulating Heart Damage and Function in a Murine Model of Acute Chagas Disease. Am J Pathol 181(1):130-40. [PubMed: 22658486]  [MGI Ref ID J:185514]

Firuzi O; Zhuo J; Chinnici CM; Wisniewski T; Pratico D. 2008. 5-Lipoxygenase gene disruption reduces amyloid-beta pathology in a mouse model of Alzheimer's disease. FASEB J 22(4):1169-78. [PubMed: 17998412]  [MGI Ref ID J:134897]

Genovese T; Mazzon E; Rossi A; Di Paola R; Cannavo G; Muia C; Crisafulli C; Bramanti P; Sautebin L; Cuzzocrea S. 2005. Involvement of 5-lipoxygenase in spinal cord injury. J Neuroimmunol 166(1-2):55-64. [PubMed: 16019083]  [MGI Ref ID J:106400]

Ghazalpour A; Wang X; Lusis AJ; Mehrabian M. 2006. Complex inheritance of the 5-lipoxygenase locus influencing atherosclerosis in mice. Genetics 173(2):943-51. [PubMed: 16624897]  [MGI Ref ID J:109792]

Gubitosi-Klug RA; Talahalli R; Du Y; Nadler JL; Kern TS. 2008. 5-Lipoxygenase, but not 12/15-lipoxygenase, contributes to degeneration of retinal capillaries in a mouse model of diabetic retinopathy. Diabetes 57(5):1387-93. [PubMed: 18346986]  [MGI Ref ID J:136092]

Guerrero AT; Verri WA Jr; Cunha TM; Silva TA; Schivo IR; Dal-Secco D; Canetti C; Rocha FA; Parada CA; Cunha FQ; Ferreira SH. 2008. Involvement of LTB4 in zymosan-induced joint nociception in mice: participation of neutrophils and PGE2. J Leukoc Biol 83(1):122-30. [PubMed: 17913976]  [MGI Ref ID J:130113]

Ichinose F; Zapol WM; Sapirstein A; Ullrich R; Tager AM; Coggins K; Jones R; Bloch KD. 2001. Attenuation of hypoxic pulmonary vasoconstriction by endotoxemia requires 5-lipoxygenase in mice. Circ Res 88(8):832-8. [PubMed: 11325876]  [MGI Ref ID J:115401]

Irvin CG; Tu YP; Sheller JR; Funk CD. 1997. 5-Lipoxygenase products are necessary for ovalbumin-induced airway responsiveness in mice. Am J Physiol 272(6 Pt 1):L1053-8. [PubMed: 9227503]  [MGI Ref ID J:113047]

Jiang W; Hall SR; Moos MP; Cao RY; Ishii S; Ogunyankin KO; Melo LG; Funk CD. 2008. Endothelial Cysteinyl Leukotriene 2 Receptor Expression Mediates Myocardial Ischemia-Reperfusion Injury. Am J Pathol 172(3):592-602. [PubMed: 18276782]  [MGI Ref ID J:132275]

Kitagawa K; Matsumoto M; Hori M. 2004. Cerebral ischemia in 5-lipoxygenase knockout mice. Brain Res 1004(1-2):198-202. [PubMed: 15033436]  [MGI Ref ID J:89268]

Le P; Kawai M; Bornstein S; DeMambro VE; Horowitz MC; Rosen CJ. 2012. A high-fat diet induces bone loss in mice lacking the Alox5 gene. Endocrinology 153(1):6-16. [PubMed: 22128029]  [MGI Ref ID J:181771]

Leedom AJ; Sullivan AB; Dong B; Lau D; Gronert K. 2010. Endogenous LXA4 circuits are determinants of pathological angiogenesis in response to chronic injury. Am J Pathol 176(1):74-84. [PubMed: 20008149]  [MGI Ref ID J:156477]

Lemos HP; Grespan R; Vieira SM; Cunha TM; Verri WA Jr; Fernandes KS; Souto FO; McInnes IB; Ferreira SH; Liew FY; Cunha FQ. 2009. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc Natl Acad Sci U S A 106(14):5954-9. [PubMed: 19289819]  [MGI Ref ID J:147573]

Liao T; Ke Y; Shao WH; Haribabu B; Kaplan HJ; Sun D; Shao H. 2006. Blockade of the interaction of leukotriene b4 with its receptor prevents development of autoimmune uveitis. Invest Ophthalmol Vis Sci 47(4):1543-9. [PubMed: 16565390]  [MGI Ref ID J:108446]

Machado ER; Ueta MT; Lourenco EV; Anibal FF; Sorgi CA; Soares EG; Roque-Barreira MC; Medeiros AI; Faccioli LH. 2005. Leukotrienes play a role in the control of parasite burden in murine strongyloidiasis. J Immunol 175(6):3892-9. [PubMed: 16148135]  [MGI Ref ID J:116694]

Mancuso P; Lewis C; Serezani CH; Goel D; Peters-Golden M. 2010. Intrapulmonary administration of leukotriene B4 enhances pulmonary host defense against pneumococcal pneumonia. Infect Immun 78(5):2264-71. [PubMed: 20231413]  [MGI Ref ID J:159878]

McKee AS; Munks MW; MacLeod MK; Fleenor CJ; Van Rooijen N; Kappler JW; Marrack P. 2009. Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J Immunol 183(7):4403-14. [PubMed: 19734227]  [MGI Ref ID J:152787]

Medeiros AI; Sa-Nunes A; Turato WM; Secatto A; Frantz FG; Sorgi CA; Serezani CH; Deepe GS Jr; Faccioli LH. 2008. Leukotrienes are potent adjuvant during fungal infection: effects on memory T cells. J Immunol 181(12):8544-51. [PubMed: 19050273]  [MGI Ref ID J:142061]

Mehrabian M; Allayee H; Stockton J; Lum PY; Drake TA; Castellani LW; Suh M; Armour C; Edwards S; Lamb J; Lusis AJ; Schadt EE. 2005. Integrating genotypic and expression data in a segregating mouse population to identify 5-lipoxygenase as a susceptibility gene for obesity and bone traits. Nat Genet 37(11):1224-33. [PubMed: 16200066]  [MGI Ref ID J:104825]

Mehrabian M; Allayee H; Wong J; Shi W; Wang XP; Shaposhnik Z; Funk CD; Lusis AJ. 2002. Identification of 5-lipoxygenase as a major gene contributing to atherosclerosis susceptibility in mice. Circ Res 91(2):120-6. [PubMed: 12142344]  [MGI Ref ID J:109703]

Mehrabian M; Schulthess FT; Nebohacova M; Castellani LW; Zhou Z; Hartiala J; Oberholzer J; Lusis AJ; Maedler K; Allayee H. 2008. Identification of ALOX5 as a gene regulating adiposity and pancreatic function. Diabetologia 51(6):978-88. [PubMed: 18421434]  [MGI Ref ID J:137920]

Monteiro AP; Pinheiro CS; Luna-Gomes T; Alves LR; Maya-Monteiro CM; Porto BN; Barja-Fidalgo C; Benjamim CF; Peters-Golden M; Bandeira-Melo C; Bozza MT; Canetti C. 2011. Leukotriene b4 mediates neutrophil migration induced by heme. J Immunol 186(11):6562-7. [PubMed: 21536805]  [MGI Ref ID J:173172]

Nagashima T; Ichimiya S; Kikuchi T; Saito Y; Matsumiya H; Ara S; Koshiba S; Zhang J; Hatate C; Tonooka A; Kubo T; Ye RC; Hirose B; Shirasaki H; Izumi T; Takami T; Himi T; Sato N. 2011. Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help. Am J Pathol 178(1):222-32. [PubMed: 21224059]  [MGI Ref ID J:168089]

Narushima S; DiMeo D; Tian J; Zhang J; Liu D; Berg DJ. 2008. 5-Lipoxygenase-derived lipid mediators are not required for the development of NSAID-induced inflammatory bowel disease in IL-10-/- mice. Am J Physiol Gastrointest Liver Physiol 294(2):G477-88. [PubMed: 18048478]  [MGI Ref ID J:132202]

Obrosova IG; Stavniichuk R; Drel VR; Shevalye H; Vareniuk I; Nadler JL; Schmidt RE. 2010. Different roles of 12/15-lipoxygenase in diabetic large and small fiber peripheral and autonomic neuropathies. Am J Pathol 177(3):1436-47. [PubMed: 20724598]  [MGI Ref ID J:163914]

Paiva LA; Maya-Monteiro CM; Bandeira-Melo C; Silva PM; El-Cheikh MC; Teodoro AJ; Borojevic R; Perez SA; Bozza PT. 2010. Interplay of cysteinyl leukotrienes and TGF-beta in the activation of hepatic stellate cells from Schistosoma mansoni granulomas. Biochim Biophys Acta 1801(12):1341-8. [PubMed: 20817008]  [MGI Ref ID J:170218]

Peebles RS Jr; Hashimoto K; Sheller JR; Moore ML; Morrow JD; Ji S; Elias JA; Goleniewska K; O'neal J; Mitchell DB; Graham BS; Zhou W. 2005. Allergen-induced airway hyperresponsiveness mediated by cyclooxygenase inhibition is not dependent on 5-lipoxygenase or IL-5, but is IL-13 dependent. J Immunol 175(12):8253-9. [PubMed: 16339565]  [MGI Ref ID J:122257]

Peng C; Chen Y; Shan Y; Zhang H; Guo Z; Li D; Li S. 2012. LSK derived LSK- cells have a high apoptotic rate related to survival regulation of hematopoietic and leukemic stem cells. PLoS One 7(6):e38614. [PubMed: 22675576]  [MGI Ref ID J:187845]

Peters-Golden M; Bailie M; Marshall T; Wilke C; Phan SH; Toews GB; Moore BB. 2002. Protection from pulmonary fibrosis in leukotriene-deficient mice. Am J Respir Crit Care Med 165(2):229-35. [PubMed: 11790660]  [MGI Ref ID J:103107]

Poeckel D; Zemski Berry KA; Murphy RC; Funk CD. 2009. Dual 12/15- and 5-lipoxygenase deficiency in macrophages alters arachidonic acid metabolism and attenuates peritonitis and atherosclerosis in apoe knock-out mice. J Biol Chem 284(31):21077-89. [PubMed: 19509298]  [MGI Ref ID J:153174]

Queto T; Gaspar-Elsas MI; Masid-de-Brito D; Vasconcelos ZF; Ferraris FK; Penido C; Cunha FQ; Kanaoka Y; Lam BK; Xavier-Elsas P. 2010. Cysteinyl-leukotriene type 1 receptors transduce a critical signal for the up-regulation of eosinophilopoiesis by interleukin-13 and eotaxin in murine bone marrow. J Leukoc Biol 87(5):885-93. [PubMed: 20219953]  [MGI Ref ID J:160350]

Rossi A; Acquaviva AM; Iuliano F; Di Paola R; Cuzzocrea S; Sautebin L. 2005. Up-regulation of prostaglandin biosynthesis by leukotriene C4 in elicited mice peritoneal macrophages activated with lipopolysaccharide/interferon-{gamma}. J Leukoc Biol 78(4):985-91. [PubMed: 16046553]  [MGI Ref ID J:101535]

Sadik CD; Kim ND; Iwakura Y; Luster AD. 2012. Neutrophils orchestrate their own recruitment in murine arthritis through C5aR and FcgammaR signaling. Proc Natl Acad Sci U S A 109(46):E3177-85. [PubMed: 23112187]  [MGI Ref ID J:191734]

Sapieha P; Stahl A; Chen J; Seaward MR; Willett KL; Krah NM; Dennison RJ; Connor KM; Aderman CM; Liclican E; Carughi A; Perelman D; Kanaoka Y; Sangiovanni JP; Gronert K; Smith LE. 2011. 5-Lipoxygenase metabolite 4-HDHA is a mediator of the antiangiogenic effect of omega-3 polyunsaturated fatty acids. Sci Transl Med 3(69):69ra12. [PubMed: 21307302]  [MGI Ref ID J:171000]

Secatto A; Rodrigues LC; Serezani CH; Ramos SG; Dias-Baruffi M; Faccioli LH; Medeiros AI. 2012. 5-Lipoxygenase deficiency impairs innate and adaptive immune responses during fungal infection. PLoS One 7(3):e31701. [PubMed: 22448213]  [MGI Ref ID J:187034]

Segal BH; Kuhns DB; Ding L; Gallin JI; Holland SM. 2002. Thioglycollate peritonitis in mice lacking C5, 5-lipoxygenase, or p47(phox): complement, leukotrienes, and reactive oxidants in acute inflammation. J Leukoc Biol 71(3):410-6. [PubMed: 11867678]  [MGI Ref ID J:75371]

Serezani CH; Aronoff DM; Jancar S; Mancuso P; Peters-Golden M. 2005. Leukotrienes enhance the bactericidal activity of alveolar macrophages against Klebsiella pneumoniae through the activation of NADPH oxidase. Blood 106(3):1067-75. [PubMed: 15718414]  [MGI Ref ID J:117309]

Serezani CH; Aronoff DM; Jancar S; Peters-Golden M. 2005. Leukotriene B4 mediates p47phox phosphorylation and membrane translocation in polyunsaturated fatty acid-stimulated neutrophils. J Leukoc Biol 78(4):976-84. [PubMed: 16006535]  [MGI Ref ID J:101536]

Serezani CH; Kane S; Collins L; Morato-Marques M; Osterholzer JJ; Peters-Golden M. 2012. Macrophage dectin-1 expression is controlled by leukotriene B4 via a GM-CSF/PU.1 axis. J Immunol 189(2):906-15. [PubMed: 22696442]  [MGI Ref ID J:189546]

Serezani CH; Lewis C; Jancar S; Peters-Golden M. 2011. Leukotriene B4 amplifies NF-kappaB activation in mouse macrophages by reducing SOCS1 inhibition of MyD88 expression. J Clin Invest 121(2):671-82. [PubMed: 21206089]  [MGI Ref ID J:171834]

Serezani CH; Perrela JH; Russo M; Peters-Golden M; Jancar S. 2006. Leukotrienes are essential for the control of Leishmania amazonensis infection and contribute to strain variation in susceptibility. J Immunol 177(5):3201-8. [PubMed: 16920959]  [MGI Ref ID J:139527]

Shim YM; Zhu Z; Zheng T; Lee CG; Homer RJ; Ma B; Elias JA. 2006. Role of 5-lipoxygenase in IL-13-induced pulmonary inflammation and remodeling. J Immunol 177(3):1918-24. [PubMed: 16849505]  [MGI Ref ID J:138359]

Soares EM; Mason KL; Rogers LM; Serezani CH; Faccioli LH; Aronoff DM. 2013. Leukotriene B4 Enhances Innate Immune Defense against the Puerperal Sepsis Agent Streptococcus pyogenes. J Immunol 190(4):1614-22. [PubMed: 23325886]  [MGI Ref ID J:193406]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129S2 background and has been backcrossed to C57BL/6J for at least nine generations(8/21/01). Coat color expected from breeding:Black
Mating System	Homozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering Information
JAX^® Mice
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.