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- Description
- Phenotype
- Genes & alleles
- Genotyping
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N2 Donating Investigator Greg Barsh, Stanford University Medical Center Description
Mice that are homozygous for this targeted allele appear weak, fail to feed and die within a few hours of birth from unspecified causes. Embryos exhibit a slightly foreshortened head and abnormal lens development. No Maf gene transcript is detected. Posterior lens cells from homozygotes fail to elongate. These cells display inappropriately high levels of DNA synthesis and express alpha A crystallin and all of the beta crystallin genes at dramatically reduced levels. Heterozygotes are phenotypically normal. The presence of lacZ in the targeted allele makes it possible to observe Maf expression characteristics.Development
A targeting vector containing neomycin resistance, lacZ and herpes simplex virus thymidine kinase genes was used to disrupt the single Maf exon. The targeting construct was designed to remove the carboxy-terminal 351 amino acids and 3kb of 3' untranslated sequence. The lacZ gene was fused to an amino-terminal portion of the Maf gene encoding 19 amino acids. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Maftm1Gsb/Maftm1Gsb
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
- lethality-prenatal/perinatal
- neonatal lethality (MGI Ref ID J:56583)
- mice die within hours of birth
- vision/eye phenotype
- abnormal cornea morphology (MGI Ref ID J:56583)
- in older mice, corneas are thickened and exhibit increased cell proliferation and irregularity at the endothelial surface
- abnormal lens development (MGI Ref ID J:56583)
- by E12.5, fiber cell differentiation and elongation ceases
- early maturation of primary and secondary lens fiber cells and the ability of these cells to remain in G0 is disrupted
- at E15.5, the boundary between proliferating and non-proliferating cells is shifted anteriorly
- abnormal lens vesicle development (MGI Ref ID J:56583)
- by E13.5, the lens vesicle is hollow whereas it is filled in wild-type mice
- by E15.5, epithelial cells are confined to the anterior third rather than the anterior half of the lens vesicle
- after E16.5, the lens vesicle is deformed
- small lens (MGI Ref ID J:56583)
- at E17.5, lens size is reduced by one half or less of wild-type
- craniofacial phenotype
- shortened head (MGI Ref ID J:56583)
- mice exhibit a fore-shortened head at birth
- behavior/neurological phenotype
- decreased eating behavior (MGI Ref ID J:56583)
- mice do not eat at birth
- weakness (MGI Ref ID J:56583)
- at birth
This mouse can be used to support research in many areas including:
Developmental Biology Research
Eye Defects
Perinatal Lethality
Homozygous
Research Tools
lacZ Expression
Sensorineural Research
Eye Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Maftm1Gsb | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Gregory S Barsh | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | MaflacZ; | ||
| Mutation Made By | Brian Ring, Stanford University | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | The presence of lacZ in the targeted allele makes it possible to observe Maf expression characteristics. | ||
| Gene Symbol and Name | Maf, avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | 2810401A20Rik; A230108G15Rik; AW047063; MGC71685; Maf2; RIKEN cDNA 2810401A20 gene; RIKEN cDNA A230108G15 gene; c-MAF; cMaf; expressed sequence AW047063; | ||
| Molecular Note | Replacement of sequences encoding the C-terminal 351 amino acids with a lacZ gene. This allele expresses a fusion protein of the first 19 amino acids of MAF fused to beta-galactosidase. [MGI Ref ID J:56583] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Maftm1Gsb, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Ring BZ; Cordes SP; Overbeek PA; Barsh GS. 2000. Regulation of mouse lens fiber cell development and differentiation by the Maf gene. Development 127(2):307-17. [PubMed: 10603348] [MGI Ref ID J:56583]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129 background and has been backcrossed to C57BL/6J for at least 5 generations(8/23/01).
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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