Strain Name:

B6.129P2-Adra1btm1Cta/J

Stock Number:

004159

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129P2-Adra1btm1Cta    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Susanna Cotecchia,   Institut de Pharmacologie et de Toxicolo

Description
Mice that are homozygous null for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Adra1b transcripts are detected. The amount of alpha 1 adrenergic recptor binding activity as measured by radioligand assays is dramatically decreased in homozygous liver, heart and cerebral cortex. The blood pressure response induced by increasing doses of phenylephrine is reduced by 45% in comparison to wild type mice. Similarly, phenylephrine-induced contraction of aortic tissue is diminished 25%. This mutant mouse strain represents a model that may be useful in studies related to blood pressure regulation.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129P2/OlaHsd-derived HM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Adra1btm1Cta/Adra1btm1Cta

        involves: 129P2/OlaHsd
  • cardiovascular system phenotype
  • abnormal heart left ventricle morphology
    • left ventricular volume is higher   (MGI Ref ID J:103581)
  • abnormal systemic arterial blood pressure
    • mean arterial blood pressure response to phenylephrine is decreased by 45%   (MGI Ref ID J:43591)
    • mean arterial blood pressure response to norepinephrine is also decreased although to a lesser extent than seen with phenylephrine   (MGI Ref ID J:43591)
    • decreased systemic arterial blood pressure
      • exhibit impaired pressor response to a hypotensive stimulus, with marked attenuation in the change in systolic blood pressure and in the percent change in the end-systolic pressure-volume relationship after transient bilateral carotid occlusion   (MGI Ref ID J:103581)
      • decreased systemic arterial systolic blood pressure
        • baseline systolic blood pressure and Ea are lower   (MGI Ref ID J:103581)
  • decreased cardiac muscle contractility
    • phenylephrine-induced contractions of aortic rings are decreased by 25%   (MGI Ref ID J:43591)
    • baseline myocardial contractility is lower, as reflected by dP/dt   (MGI Ref ID J:103581)
    • transient bilateral carotid occlusion results in a minimal increase in myocardial contractility instead of the enhanced contractility seen in wild-type   (MGI Ref ID J:103581)
  • decreased heart rate
    • transient bilateral carotid occlusion results in a decrease in heart rate compared to a slight increase in treated wild-type, however baseline heart rate is normal   (MGI Ref ID J:103581)
  • decreased vasoconstriction
    • exhibit a reduction in mesenteric vascular responses to endogenous norepinephrine compared to wild-type, indicating reduced vasoconstriction in response to norepinephrine   (MGI Ref ID J:103581)
  • muscle phenotype
  • decreased cardiac muscle contractility
    • phenylephrine-induced contractions of aortic rings are decreased by 25%   (MGI Ref ID J:43591)
    • baseline myocardial contractility is lower, as reflected by dP/dt   (MGI Ref ID J:103581)
    • transient bilateral carotid occlusion results in a minimal increase in myocardial contractility instead of the enhanced contractility seen in wild-type   (MGI Ref ID J:103581)
  • decreased vasoconstriction
    • exhibit a reduction in mesenteric vascular responses to endogenous norepinephrine compared to wild-type, indicating reduced vasoconstriction in response to norepinephrine   (MGI Ref ID J:103581)
  • behavior/neurological phenotype
  • decreased exploration in new environment
    • 4-5 month old homozygotes show significantly reduced horizontal exploratory activity and a reduced rearing behavior (vertical activity) in the open field   (MGI Ref ID J:102553)
  • impaired passive avoidance behavior
    • 4-5 month old homozygotes show impaired retention of an inhibitory avoidance task after receiving a stressful electric stimuli   (MGI Ref ID J:102553)
  • nervous system phenotype
  • abnormal baroreceptor physiology
    • transient bilateral carotid occlusion to test cardiovascular responses to a hypotensive stimuli produced an attenuated pressor response, indicating an abnormal baroreflex activity   (MGI Ref ID J:103581)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Adra1btm1Cta related

Cardiovascular Research
Heart Abnormalities
Hypotension
Vascular Defects

Internal/Organ Research
Heart Abnormalities

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Adra1btm1Cta
Allele Name targeted mutation 1, Susanna Cotecchia
Allele Type Targeted (Null/Knockout)
Common Name(s) alpha1b-; alpha1b-AR-;
Mutation Made By Susanna Cotecchia,   Institut de Pharmacologie et de Toxicolo
Strain of Origin129P2/OlaHsd-Hprt
ES Cell Line NameHM-1
ES Cell Line Strain129P2/OlaHsd-Hprt
Gene Symbol and Name Adra1b, adrenergic receptor, alpha 1b
Chromosome 11
Gene Common Name(s) ADRA1; ALPHA1BAR; alpha1B-adrenergic receptor;
Molecular Note A genomic fragment containing exon 1 was deleted and replaced with a neomycin selection cassette. RT-PCR analysis confirmed that no transcript is produced from this allele. [MGI Ref ID J:43591]

Genotyping

Genotyping Information

Genotyping Protocols

Adra1btm1Cta, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Adra1btm1Cta related

Auclair A; Cotecchia S; Glowinski J; Tassin JP. 2002. D-amphetamine fails to increase extracellular dopamine levels in mice lacking alpha 1b-adrenergic receptors: relationship between functional and nonfunctional dopamine release. J Neurosci 22(21):9150-4. [PubMed: 12417637]  [MGI Ref ID J:123996]

Battaglia G; Fornai F; Busceti CL; Lembo G; Nicoletti F; De Blasi A. 2003. Alpha-1B adrenergic receptor knockout mice are protected against methamphetamine toxicity. J Neurochem 86(2):413-21. [PubMed: 12871582]  [MGI Ref ID J:84429]

Burcelin R; Uldry M; Foretz M; Perrin C; Dacosta A; Nenniger-Tosato M; Seydoux J; Cotecchia S; Thorens B. 2004. Impaired glucose homeostasis in mice lacking the alpha1b-adrenergic receptor subtype. J Biol Chem 279(2):1108-15. [PubMed: 14581480]  [MGI Ref ID J:87718]

Cavalli A; Lattion AL; Hummler E; Nenniger M; Pedrazzini T; Aubert JF; Michel MC; Yang M; Lembo G; Vecchione C; Mostardini M; Schmidt A; Beermann F; Cotecchia S. 1997. Decreased blood pressure response in mice deficient of the alpha1b-adrenergic receptor. Proc Natl Acad Sci U S A 94(21):11589-94. [PubMed: 9326654]  [MGI Ref ID J:43591]

Drouin C; Darracq L; Trovero F; Blanc G; Glowinski J; Cotecchia S; Tassin JP. 2002. Alpha1b-adrenergic receptors control locomotor and rewarding effects of psychostimulants and opiates. J Neurosci 22(7):2873-84. [PubMed: 11923452]  [MGI Ref ID J:76004]

Faber JE; Szymeczek CL; Cotecchia S; Thomas SA; Tanoue A; Tsujimoto G; Zhang H. 2007. Alpha1-adrenoceptor-dependent vascular hypertrophy and remodeling in murine hypoxic pulmonary hypertension. Am J Physiol Heart Circ Physiol 292(5):H2316-23. [PubMed: 17220188]  [MGI Ref ID J:125945]

Gericke A; Kordasz ML; Steege A; Sanbe A; Goloborodko E; Vetter JM; Patzak A; Pfeiffer N. 2011. Functional role of alpha1-adrenoceptor subtypes in murine ophthalmic arteries. Invest Ophthalmol Vis Sci 52(7):4795-9. [PubMed: 21613371]  [MGI Ref ID J:181413]

Hosoda C; Hiroyama M; Sanbe A; Birumachi J; Kitamura T; Cotecchia S; Simpson PC; Tsujimoto G; Tanoue A. 2007. Blockade of both alpha1A- and alpha1B-adrenergic receptor subtype signaling is required to inhibit neointimal formation in the mouse femoral artery. Am J Physiol Heart Circ Physiol 293(1):H514-9. [PubMed: 17384126]  [MGI Ref ID J:126035]

Hosoda C; Koshimizu TA; Tanoue A; Nasa Y; Oikawa R; Tomabechi T; Fukuda S; Shinoura H; Oshikawa S; Takeo S; Kitamura T; Cotecchia S; Tsujimoto G. 2005. Two alpha1-adrenergic receptor subtypes regulating the vasopressor response have differential roles in blood pressure regulation. Mol Pharmacol 67(3):912-22. [PubMed: 15598970]  [MGI Ref ID J:110125]

Huang Y; Wright CD; Merkwan CL; Baye NL; Liang Q; Simpson PC; O'Connell TD. 2007. An alpha1A-adrenergic-extracellular signal-regulated kinase survival signaling pathway in cardiac myocytes. Circulation 115(6):763-72. [PubMed: 17283256]  [MGI Ref ID J:132328]

Knauber J; Muller WE. 2000. Decreased exploratory activity and impaired passive avoidance behaviour in mice deficient for the alpha(1b)-adrenoceptor. Eur Neuropsychopharmacol 10(6):423-7. [PubMed: 11115730]  [MGI Ref ID J:102553]

Koshimizu T; Tanoue A; Tsujimimoto G. 2007. Clinical implications from studies of alpha1 adrenergic receptor knockout mice Biochem Pharmacol 73(8):1107-12. [PubMed: 17141736]  [MGI Ref ID J:117338]

McCloskey DT; Turnbull L; Swigart P; O'Connell TD; Simpson PC; Baker AJ. 2003. Abnormal myocardial contraction in alpha(1A)- and alpha(1B)-adrenoceptor double-knockout mice. J Mol Cell Cardiol 35(10):1207-16. [PubMed: 14519431]  [MGI Ref ID J:102648]

Mhaouty-Kodja S; Lozach A; Habert R; Tanneux M; Guigon C; Brailly-Tabard S; Maltier JP; Legrand-Maltier C. 2007. Fertility and spermatogenesis are altered in {alpha}1b-adrenergic receptor knockout male mice. J Endocrinol 195(2):281-92. [PubMed: 17951539]  [MGI Ref ID J:125763]

O'Connell TD; Ishizaka S; Nakamura A; Swigart PM; Rodrigo MC; Simpson GL; Cotecchia S; Rokosh DG; Grossman W; Foster E; Simpson PC. 2003. The alpha(1A/C)- and alpha(1B)-adrenergic receptors are required for physiological cardiac hypertrophy in the double-knockout mouse. J Clin Invest 111(11):1783-91. [PubMed: 12782680]  [MGI Ref ID J:83876]

O'Connell TD; Swigart PM; Rodrigo MC; Ishizaka S; Joho S; Turnbull L; Tecott LH; Baker AJ; Foster E; Grossman W; Simpson PC. 2006. Alpha1-adrenergic receptors prevent a maladaptive cardiac response to pressure overload. J Clin Invest 116(4):1005-15. [PubMed: 16585965]  [MGI Ref ID J:107811]

Pizzanelli C; Lazzeri G; Fulceri F; Giorgi FS; Pasquali L; Cifelli G; Murri L; Fornai F. 2009. Lack of alpha 1b-adrenergic receptor protects against epileptic seizures. Epilepsia 50 Suppl 1:59-64. [PubMed: 19125850]  [MGI Ref ID J:166113]

Salomon L; Lanteri C; Glowinski J; Tassin JP. 2006. Behavioral sensitization to amphetamine results from an uncoupling between noradrenergic and serotonergic neurons. Proc Natl Acad Sci U S A 103(19):7476-81. [PubMed: 16648258]  [MGI Ref ID J:109449]

Sanbe A; Tanaka Y; Fujiwara Y; Tsumura H; Yamauchi J; Cotecchia S; Koike K; Tsujimoto G; Tanoue A. 2007. Alpha1-adrenoceptors are required for normal male sexual function. Br J Pharmacol 152(3):332-40. [PubMed: 17603545]  [MGI Ref ID J:147536]

Spreng M; Cotecchia S; Schenk F. 2001. A behavioral study of alpha-1b adrenergic receptor knockout mice: increased reaction to novelty and selectively reduced learning capacities. Neurobiol Learn Mem 75(2):214-29. [PubMed: 11222061]  [MGI Ref ID J:68177]

Szondy Z; Mastroberardino PG; Varadi J; Farrace MG; Nagy N; Bak I; Viti I; Wieckowski MR; Melino G; Rizzuto R; Tosaki A; Fesus L; Piacentini M. 2006. Tissue transglutaminase (TG2) protects cardiomyocytes against ischemia/reperfusion injury by regulating ATP synthesis. Cell Death Differ 13(10):1827-9. [PubMed: 16528383]  [MGI Ref ID J:126414]

Townsend SA; Jung AS; Hoe YS; Lefkowitz RY; Khan SA; Lemmon CA; Harrison RW; Lee K; Barouch LA; Cotecchia S; Shoukas AA; Nyhan D; Hare JM; Berkowitz DE. 2004. Critical role for the alpha-1B adrenergic receptor at the sympathetic neuroeffector junction. Hypertension 44(5):776-82. [PubMed: 15466664]  [MGI Ref ID J:103581]

Turnbull L; McCloskey DT; O'Connell TD; Simpson PC; Baker AJ. 2003. Alpha 1-adrenergic receptor responses in alpha 1AB-AR knockout mouse hearts suggest the presence of alpha 1D-AR. Am J Physiol Heart Circ Physiol 284(4):H1104-9. [PubMed: 12595294]  [MGI Ref ID J:83036]

Vecchione C; Fratta L; Rizzoni D; Notte A; Poulet R; Porteri E; Frati G; Guelfi D; Trimarco V; Mulvany MJ; Agabiti-Rosei E; Trimarco B; Cotecchia S; Lembo G. 2002. Cardiovascular influences of alpha1b-adrenergic receptor defect in mice. Circulation 105(14):1700-7. [PubMed: 11940550]  [MGI Ref ID J:128670]

Zhang H; Cotecchia S; Thomas SA; Tanoue A; Tsujimoto G; Faber JE. 2004. Gene deletion of dopamine beta-hydroxylase and alpha1-adrenoceptors demonstrates involvement of catecholamines in vascular remodeling. Am J Physiol Heart Circ Physiol 287(5):H2106-14. [PubMed: 15231500]  [MGI Ref ID J:95771]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129P2 background and has been backcrossed to C57BL/6 for at least seven generations.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)