Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F?+2p   Donating Investigator Elaine Fuchs,   The Rockefeller University

Description
Mice homozygous for this targeted allele are viable, fertile and normal in size. No Fgf7 transcript is detected. By 2 months of age, the hair coat takes on a matted/greasy appearance that becomes more prominent with age. Homozygote kidneys are markedly smaller and posses structural anomalies. Morphometric analyses indicate a reduction (~30% of wildtype) in the total number of nephrons present. Kidney development appears to be effected as early as embryonic day 16.5. This mutant mouse strain represents a model that may be useful in studies related to kidney disease.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were mated to C57BL/6 mice.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Fgf7^tm1Efu/Fgf7^tm1Efu

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• skin/coat/nails phenotype
• *normal* skin/coat/nails phenotype (MGI Ref ID J:31155)
  • no major abnormalities in hair structure or hair types
  • hair lengths normal
• abnormal coat appearance (MGI Ref ID J:31155)
  • perturbation in the direction of hairs within the coat
• disheveled coat (MGI Ref ID J:31155)
• greasy coat (MGI Ref ID J:31155)
  • no excess of oil or greasy substances
  • by 2 months in males
  • by 1 year in females
  • phenotype becomes more prominent with age
• matted coat (MGI Ref ID J:31155)
  • by 2 months in males
  • by 1 year in females
  • phenotype becomes more prominent with age
• renal/urinary system phenotype
• abnormal kidney collecting duct (MGI Ref ID J:53739)
  • epithelium of collecting ducts is low cuboid rather than high cuboid or columnar
  • functionally normal
• abnormal kidney cortex (MGI Ref ID J:53739)
  • thin
• abnormal kidney development (MGI Ref ID J:53739)
  • normal from E11.5 to E13.5, after 3-4 rounds of branching
• abnormal ureteric bud branching morphogenesis (MGI Ref ID J:53739)
  • few ureteric bud branches in the inner medullary region at E16.5
• abnormal kidney epithelium morphology (MGI Ref ID J:53739)
  • epithelium of collecting ducts is low cuboid rather than high cuboid or columnar
• abnormal kidney medulla morphology (MGI Ref ID J:53739)
  • few ureteric bud branches in the inner medullary region at E16.5
• abnormal kidney papilla morphology (MGI Ref ID J:53739)
  • thin with fewer papillary collecting ducts
• abnormal nephron morphology (MGI Ref ID J:53739)
  • 30% reduction in the number of nephrons but density normal
• small kidney (MGI Ref ID J:53739)
  • kidneys are small while body size is normal
• homeostasis/metabolism phenotype
• *normal* homeostasis/metabolism phenotype (MGI Ref ID J:31155)
  • wound healing is normal
• reproductive system phenotype
• *normal* reproductive system phenotype (MGI Ref ID J:31155)
  • testes and seminal vesicles are normal

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Fgf7^tm1Efu related

Cancer Research
Genes Regulating Growth and Proliferation

Cell Biology Research
Defects in Cell Adhesion Molecules
Genes Regulating Growth and Proliferation

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Defects in Cell Adhesion Molecules
Growth Defects
Internal/Organ Defects (kidney)
Internal/Organ Defects (lung)
Internal/Organ Defects (prostate)
Skin and Hair Texture Defects

Endocrine Deficiency Research
Kidney Defects
Skin Defects

Internal/Organ Research
Kidney Defects
Lung Defects
Prostate

Research Tools
Dermatology Research
Internal/Organ Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Fgf7tm1Efu
Allele Name		targeted mutation 1, Elaine Fuchs
Allele Type		Targeted (knock-out)
Common Name(s)		FGF7-; KGF-;
Mutation Made By		Linda Degenstein,   The University of Chicago
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Fgf7, fibroblast growth factor 7
Chromosome		2
Gene Common Name(s)		HBGF-7; KGF; Keratinocyte growth factor;
Molecular Note		Replacement of 205bp in exon 1 with a neomycin resistance cassette. [MGI Ref ID J:31155]

Genotyping

Genotyping Information

Genotyping Protocols

Fgf7^tm1Efu, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Guo L; Degenstein L; Fuchs E. 1996. Keratinocyte growth factor is required for hair development but not for wound healing. Genes Dev 10(2):165-75. [PubMed: 8566750]  [MGI Ref ID J:31155]

Additional References

Qiao J; Uzzo R; Obara-Ishihara T; Degenstein L; Fuchs E; Herzlinger D. 1999. FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development 126(3):547-54. [PubMed: 9876183]  [MGI Ref ID J:53739]

Fgf7^tm1Efu related

Alpdogan O; Hubbard VM; Smith OM; Patel N; Lu S; Goldberg GL; Gray DH; Feinman J; Kochman AA; Eng JM; Suh D; Muriglan SJ; Boyd RL; van den Brink MR. 2006. Keratinocyte growth factor (KGF) is required for postnatal thymic regeneration. Blood 107(6):2453-60. [PubMed: 16304055]  [MGI Ref ID J:129368]

Chen Y; Chou K; Fuchs E; Havran WL; Boismenu R. 2002. Protection of the intestinal mucosa by intraepithelial gamma delta T cells. Proc Natl Acad Sci U S A 99(22):14338-43. [PubMed: 12376619]  [MGI Ref ID J:125077]

Neuhaus P; Oustanina S; Loch T; Kruger M; Bober E; Dono R; Zeller R; Braun T. 2003. Reduced mobility of fibroblast growth factor (FGF)-deficient myoblasts might contribute to dystrophic changes in the musculature of FGF2/FGF6/mdx triple-mutant mice. Mol Cell Biol 23(17):6037-48. [PubMed: 12917328]  [MGI Ref ID J:85088]

Qiao J; Uzzo R; Obara-Ishihara T; Degenstein L; Fuchs E; Herzlinger D. 1999. FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development 126(3):547-54. [PubMed: 9876183]  [MGI Ref ID J:53739]

Sharp LL; Jameson JM; Cauvi G; Havran WL. 2005. Dendritic epidermal T cells regulate skin homeostasis through local production of insulin-like growth factor 1. Nat Immunol 6(1):73-9. [PubMed: 15592472]  [MGI Ref ID J:94872]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129 background. It is maintained on the same background by homozygous matings.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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