Strain Name:

B6;129-Fgf7tm1Efu/J

Stock Number:

004161

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Availability:

Cryopreserved - Ready for recovery

KGF knockout mice are fibroblast growth factor 7 deficient, and may be useful in studying kidney disease as well as spleen hypoplasia, abnormal synaptic vesicle clustering and miniature inhibitory postsynaptic currents, increased susceptibility to drug-induced seizures, and impaired thymic recovery after injury.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129-Fgf7tm1Efu    (Changed: 06-AUG-10 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Elaine Fuchs,   The Rockefeller University

Description
Mice homozygous for this targeted allele are viable, fertile and normal in size. No Fgf7 transcript is detected. By two months of age, the hair coat takes on a matted/greasy appearance that becomes more prominent with age. Homozygote kidneys are markedly smaller and possess structural anomalies. Morphometric analyses indicate a reduction (~30% of wildtype) in the total number of nephrons present. Kidney development appears to be effected as early as embryonic day 16.5. This mutant mouse strain represents a model that may be useful in studies related to kidney disease.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 1. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were mated to C57BL/6 mice.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Fgf7tm1Efu/Fgf7tm1Efu

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • renal/urinary system phenotype
  • abnormal kidney collecting duct epithelium morphology
    • epithelium of collecting ducts is low cuboid rather than high cuboid or columnar   (MGI Ref ID J:53739)
    • functionally normal   (MGI Ref ID J:53739)
  • abnormal kidney development
    • normal from E11.5 to E13.5, after 3-4 rounds of branching   (MGI Ref ID J:53739)
  • abnormal kidney inner medulla morphology
    • few ureteric bud branches in the inner medullary region at E16.5   (MGI Ref ID J:53739)
  • abnormal papillary duct morphology
    • fewer papillary collecting ducts   (MGI Ref ID J:53739)
  • decreased nephron number
    • 30% reduction in the number of nephrons but density normal   (MGI Ref ID J:53739)
  • impaired branching involved in ureteric bud morphogenesis
    • few ureteric bud branches in the inner medullary region at E16.5   (MGI Ref ID J:53739)
  • kidney cortex hypoplasia
  • kidney papillary hypoplasia
  • small kidney
    • kidneys are small while body size is normal   (MGI Ref ID J:53739)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • wound healing is normal   (MGI Ref ID J:31155)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • testes and seminal vesicles are normal   (MGI Ref ID J:31155)
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype
    • peripheral lymph nodes show no difference in cellularity or T-cell distribution relative to controls   (MGI Ref ID J:129368)
    • bone marrow has similar numbers of hematopoietic precursors as in wild-type   (MGI Ref ID J:129368)
    • spleen hypoplasia
      • cellularity is reduced compared to wild-type and heterozygous controls with lower absolute numbers of T cells, B cells and dendritic cells   (MGI Ref ID J:129368)
  • immune system phenotype
  • *normal* immune system phenotype
    • thymopoeisis and T-cell development are normal in mutants   (MGI Ref ID J:129368)
    • abnormal immune system physiology
      • after sublethal irradiation, thymic cellularity and thymic subpopulations are significantly lower than in controls at 21 and 28 days after irradiation indicating defective thymic recovery   (MGI Ref ID J:129368)
      • abnormal response to transplant
        • irradiated mice transplanted with allogeneic bone marrow from wild-type mice have decreased numbers of host- and donor-derived T cells compared to wild-type   (MGI Ref ID J:129368)
    • spleen hypoplasia
      • cellularity is reduced compared to wild-type and heterozygous controls with lower absolute numbers of T cells, B cells and dendritic cells   (MGI Ref ID J:129368)
  • integument phenotype
  • *normal* integument phenotype
    • no major abnormalities in hair structure or hair types   (MGI Ref ID J:31155)
    • hair lengths normal   (MGI Ref ID J:31155)
    • abnormal coat appearance
      • perturbation in the direction of hairs within the coat   (MGI Ref ID J:31155)
      • disheveled coat   (MGI Ref ID J:31155)
      • greasy coat
        • no excess of oil or greasy substances   (MGI Ref ID J:31155)
        • by 2 months in males   (MGI Ref ID J:31155)
        • by 1 year in females   (MGI Ref ID J:31155)
        • phenotype becomes more prominent with age   (MGI Ref ID J:31155)
      • matted coat
        • by 2 months in males   (MGI Ref ID J:31155)
        • by 1 year in females   (MGI Ref ID J:31155)
        • phenotype becomes more prominent with age   (MGI Ref ID J:31155)

Fgf7tm1Efu/Fgf7tm1Efu

        involves: 129S1/Sv * 129X1/SvJ
  • nervous system phenotype
  • abnormal miniature inhibitory postsynaptic currents
    • the frequency of miniature inhibitory postsynaptic currents (mIPSCs) is decreased compared to in wild-type hippocampal cultures   (MGI Ref ID J:161954)
    • however, the amplitude of mIPSCs is normal   (MGI Ref ID J:161954)
  • abnormal synaptic vesicle clustering
    • at P14, vesicle clustering in GABAergic synapses is decreased in the CA3 compared to in wild-type mice   (MGI Ref ID J:161954)
    • CA3 pyramidal neurons cultured for 14 days have fewer GABAergic vesicles associated with dendrites compared to in cultured wild-type neurons   (MGI Ref ID J:161954)
    • however, vesicle clustering in glutamatergic synapses is normal   (MGI Ref ID J:161954)
  • abnormal synaptic vesicle morphology
    • at symmetric synapses, synaptic vesicles are smaller than in wild-type mice   (MGI Ref ID J:161954)
    • abnormal synaptic vesicle number
      • fewer vesicles are docked at symmetric synapses compared to in wild-type mice   (MGI Ref ID J:161954)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Fgf7tm1Efu/Fgf7tm1Efu

        B6.129-Fgf7tm1Efu
  • behavior/neurological phenotype
  • increased susceptibility to pharmacologically induced seizures
    • pentylenetetrazol-treated mice develop major seizures sooner than similarly treated wild-type mice   (MGI Ref ID J:161954)
  • nervous system phenotype
  • increased susceptibility to pharmacologically induced seizures
    • pentylenetetrazol-treated mice develop major seizures sooner than similarly treated wild-type mice   (MGI Ref ID J:161954)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Fgf7tm1Efu related

Cancer Research
Genes Regulating Growth and Proliferation

Cell Biology Research
Defects in Cell Adhesion Molecules
Genes Regulating Growth and Proliferation

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Defects in Cell Adhesion Molecules
Growth Defects
Internal/Organ Defects
      kidney
      lung
      prostate
Skin and Hair Texture Defects

Endocrine Deficiency Research
Kidney Defects
Skin Defects

Internal/Organ Research
Kidney Defects
Lung Defects
Prostate

Research Tools
Dermatology Research
Internal/Organ Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Fgf7tm1Efu
Allele Name targeted mutation 1, Elaine Fuchs
Allele Type Targeted (knock-out)
Common Name(s) FGF7-; Fgftm1Efu; KGF-;
Mutation Made By Linda Degenstein,   The University of Chicago
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Fgf7, fibroblast growth factor 7
Chromosome 2
Gene Common Name(s) HBGF-7; KGF; Keratinocyte growth factor;
Molecular Note Replacement of 205bp in exon 1 with a neomycin resistance cassette. [MGI Ref ID J:31155]

Genotyping

Genotyping Information

Genotyping Protocols

Fgf7tm1Efu, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Guo L; Degenstein L; Fuchs E. 1996. Keratinocyte growth factor is required for hair development but not for wound healing. Genes Dev 10(2):165-75. [PubMed: 8566750]  [MGI Ref ID J:31155]

Additional References

Qiao J; Uzzo R; Obara-Ishihara T; Degenstein L; Fuchs E; Herzlinger D. 1999. FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development 126(3):547-54. [PubMed: 9876183]  [MGI Ref ID J:53739]

Fgf7tm1Efu related

Alpdogan O; Hubbard VM; Smith OM; Patel N; Lu S; Goldberg GL; Gray DH; Feinman J; Kochman AA; Eng JM; Suh D; Muriglan SJ; Boyd RL; van den Brink MR. 2006. Keratinocyte growth factor (KGF) is required for postnatal thymic regeneration. Blood 107(6):2453-60. [PubMed: 16304055]  [MGI Ref ID J:129368]

Chen Y; Chou K; Fuchs E; Havran WL; Boismenu R. 2002. Protection of the intestinal mucosa by intraepithelial gamma delta T cells. Proc Natl Acad Sci U S A 99(22):14338-43. [PubMed: 12376619]  [MGI Ref ID J:125077]

Goldberg GL; Alpdogan O; Muriglan SJ; Hammett MV; Milton MK; Eng JM; Hubbard VM; Kochman A; Willis LM; Greenberg AS; Tjoe KH; Sutherland JS; Chidgey A; van den Brink MR; Boyd RL. 2007. Enhanced immune reconstitution by sex steroid ablation following allogeneic hemopoietic stem cell transplantation. J Immunol 178(11):7473-84. [PubMed: 17513799]  [MGI Ref ID J:147822]

Lee CH; Javed D; Althaus AL; Parent JM; Umemori H. 2012. Neurogenesis is enhanced and mossy fiber sprouting arises in FGF7-deficient mice during development. Mol Cell Neurosci 51(3-4):61-7. [PubMed: 22889808]  [MGI Ref ID J:203684]

Neuhaus P; Oustanina S; Loch T; Kruger M; Bober E; Dono R; Zeller R; Braun T. 2003. Reduced mobility of fibroblast growth factor (FGF)-deficient myoblasts might contribute to dystrophic changes in the musculature of FGF2/FGF6/mdx triple-mutant mice. Mol Cell Biol 23(17):6037-48. [PubMed: 12917328]  [MGI Ref ID J:85088]

Qiao J; Uzzo R; Obara-Ishihara T; Degenstein L; Fuchs E; Herzlinger D. 1999. FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development 126(3):547-54. [PubMed: 9876183]  [MGI Ref ID J:53739]

Sharp LL; Jameson JM; Cauvi G; Havran WL. 2005. Dendritic epidermal T cells regulate skin homeostasis through local production of insulin-like growth factor 1. Nat Immunol 6(1):73-9. [PubMed: 15592472]  [MGI Ref ID J:94872]

Takase HM; Itoh T; Ino S; Wang T; Koji T; Akira S; Takikawa Y; Miyajima A. 2013. FGF7 is a functional niche signal required for stimulation of adult liver progenitor cells that support liver regeneration. Genes Dev 27(2):169-81. [PubMed: 23322300]  [MGI Ref ID J:193313]

Terauchi A; Johnson-Venkatesh EM; Toth AB; Javed D; Sutton MA; Umemori H. 2010. Distinct FGFs promote differentiation of excitatory and inhibitory synapses. Nature 465(7299):783-7. [PubMed: 20505669]  [MGI Ref ID J:161954]

Vega-Hernandez M; Kovacs A; De Langhe S; Ornitz DM. 2011. FGF10/FGFR2b signaling is essential for cardiac fibroblast development and growth of the myocardium. Development 138(15):3331-40. [PubMed: 21750042]  [MGI Ref ID J:175538]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129 background. The live colony is maintained on the same background by homozygous matings.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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