| 
| ||||||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6;129-Scaptm1Gsn/J (Changed: 04-MAY-06 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Jay D. Horton, Univ of Texas SW Med Center at Dallas Description
Mice that are homozygous for this targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. They posses a loxP-flanked neomycin resistance cassette located 3 kb upstream of exon 1. A third loxP site is located in intron 1. When used in conjunction with a Cre recombinase-expressing strain, this strain is appropriate for generating tissue-specific knockout mutants of Scap. This mutant mouse strain represents a model that may be useful in studies related to cholesterol and fatty acid homeostasis.Development
A targeting vector containing a loxP-flanked neo cassette located 3kb 5' of Scap exon 1 and a third loxP site located in intron 1, was electroporated into 129S6/SvEv-derived SM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were mated to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Scaptm1Mbjg relatedResearch Tools
Cre-lox System
loxP-flanked Sequences
Metabolism Research
Lipid Metabolism
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Scaptm1Mbjg | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joseph L Goldstein | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | SCAPflox; Scap-; Scaptm1Gsn; | ||
| Mutation Made By | Dr. Jay Horton, Univ of Texas SW Med Center at Dallas | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | SM1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Scap, SREBF chaperone | ||
| Chromosome | 9 | ||
| Molecular Note | A a loxP-flanked neomycin resistance cassette located 3 kb upstream of exon 1. An additional loxP site was inserted in intron 1. [MGI Ref ID J:69592] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Scaptm1Mbjg, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Matsuda M; Korn BS; Hammer RE; Moon YA; Komuro R; Horton JD; Goldstein JL; Brown MS; Shimomura I. 2001. SREBP cleavage-activating protein (SCAP) is required for increased lipid synthesis in liver induced by cholesterol deprivation and insulin elevation. Genes Dev 15(10):1206-16. [PubMed: 11358865] [MGI Ref ID J:69592]
Additional References
Scaptm1Mbjg relatedBesnard V; Wert SE; Stahlman MT; Postle AD; Xu Y; Ikegami M; Whitsett JA. 2009. Deletion of Scap in alveolar type II cells influences lung lipid homeostasis and identifies a compensatory role for pulmonary lipofibroblasts. J Biol Chem 284(6):4018-30. [PubMed: 19074148] [MGI Ref ID J:147592]
Horton JD; Shah NA; Warrington JA; Anderson NN; Park SW; Brown MS; Goldstein JL. 2003. Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes. Proc Natl Acad Sci U S A 100(21):12027-32. [PubMed: 14512514] [MGI Ref ID J:86192]
Kuriyama H; Liang G; Engelking LJ; Horton JD; Goldstein JL; Brown MS. 2005. Compensatory increase in fatty acid synthesis in adipose tissue of mice with conditional deficiency of SCAP in liver. Cell Metab 1(1):41-51. [PubMed: 16054043] [MGI Ref ID J:129815]
Li J; Nanayakkara A; Jun J; Savransky V; Polotsky VY. 2007. Effect of deficiency in SREBP cleavage-activating protein on lipid metabolism during intermittent hypoxia. Physiol Genomics 31(2):273-80. [PubMed: 17666524] [MGI Ref ID J:128622]
Moon YA; Liang G; Xie X; Frank-Kamenetsky M; Fitzgerald K; Koteliansky V; Brown MS; Goldstein JL; Horton JD. 2012. The Scap/SREBP pathway is essential for developing diabetic fatty liver and carbohydrate-induced hypertriglyceridemia in animals. Cell Metab 15(2):240-6. [PubMed: 22326225] [MGI Ref ID J:182284]
Rudolph MC; Monks J; Burns V; Phistry M; Marians R; Foote MR; Bauman DE; Anderson SM; Neville MC. 2010. Sterol regulatory element binding protein and dietary lipid regulation of fatty acid synthesis in the mammary epithelium. Am J Physiol Endocrinol Metab 299(6):E918-27. [PubMed: 20739508] [MGI Ref ID J:170224]
Verdier V; Csardi G; de Preux-Charles AS; Medard JJ; Smit AB; Verheijen MH; Bergmann S; Chrast R. 2012. Aging of myelinating glial cells predominantly affects lipid metabolism and immune response pathways. Glia 60(5):751-60. [PubMed: 22337502] [MGI Ref ID J:181551]
Verheijen MH; Camargo N; Verdier V; Nadra K; de Preux Charles AS; Medard JJ; Luoma A; Crowther M; Inouye H; Shimano H; Chen S; Brouwers JF; Helms JB; Feltri ML; Wrabetz L; Kirschner D; Chrast R; Smit AB. 2009. SCAP is required for timely and proper myelin membrane synthesis. Proc Natl Acad Sci U S A 106(50):21383-8. [PubMed: 19948958] [MGI Ref ID J:155526]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129S6 background. It is maintained as a homozygote on the same background. Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
This strain is currently On Hold.
To request more information use the Customer Support Contact Form or call 1-800-422-6423 or 1-207-288-5845
View All Strains Awaiting Transfer from the Donor, In Progress and On Hold
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
- 3/22/13: This strain is on hold while cryopreserved samples are being restocked.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.