Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N6p   Donating Investigator Frank Gonzalez,   National Institutes of Health

Description
Mice homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Ephx2 gene product (mRNA or protein) is detected. Systolic blood pressure is minimally effected in homozygous females but males exhibit markedly lower blood pressure. Both sexes also display an altered arachidonic acid metabolism; renal formation of dihydroxyeicosatrienoic acid is significantly diminished. This mutant mouse strain represents a model that may be useful in studies related to blood pressure regulation.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Ephx2 gene encoding exon 1. The construct was electroporated into 129X1/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were mated to C57BL/6 mice.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Ephx2^tm1Gonz/Ephx2^tm1Gonz

        involves: 129X1/SvJ * C57BL/6

• cardiovascular system phenotype
• decreased blood pressure (MGI Ref ID J:71007)
  • male homozygotes exhibit reduced systolic blood pressure both in the absence and presence of dietary salt loading compared with sex matched, wild-type mice
  • in contrast, female homozygotes show minimal differences in systolic blood pressure relative to wild-type mice
  • both mutant and wild-type female mice exhibit significantly lower systolic blood pressure than male wild-type mice
• homeostasis/metabolism phenotype
• abnormal metabolism (MGI Ref ID J:71007)
  • homozygotes of both sexes exhibit impaired hepatic and renal arachidonic acid metabolism
  • homozygotes display a 58-fold greater ratio of epoxyeicosatrienoic/dihydroxyeicosatrienoic acid (EETs/DHETs) specific activity in hepatic S-9 fractions relative to wild-type mice
  • also, homozygotes show a significant reduction in DHETs formation by kidney S-9 fractions, regardless of sex and diet

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Ephx2^tm1Gonz related

Cardiovascular Research
Hypotension

Genes & Alleles

Gene & Allele Information

Allele Symbol		Ephx2tm1Gonz
Allele Name		targeted mutation 1, Frank Gonzalez
Allele Type		Targeted (knock-out)
Common Name(s)		sEH-null;
Mutation Made By		Frank Gonzalez,   National Institutes of Health
Strain of Origin		129X1/SvJ
Gene Symbol and Name		Ephx2, epoxide hydrolase 2, cytoplasmic
Chromosome		14
Gene Common Name(s)		AW106936; CEH; Eph2; SEH; epoxide hydrolase 2, soluble; expressed sequence AW106936;
Molecular Note		Insertion of a neomycin resistance cassette into the translation initiation site in the first exon. Northern analysis did not detect transcript in liver from homozygous mutant mice. Immunoblot analysis did not detect protein in cytosolic fractions of liver from homozygous mutant mice. [MGI Ref ID J:71007]

Genotyping

Genotyping Information

Genotyping Protocols

Ephx2^tm1Gonz, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Sinal CJ; Miyata M; Tohkin M; Nagata K; Bend JR; Gonzalez FJ. 2000. Targeted disruption of soluble epoxide hydrolase reveals a role in blood pressure regulation. J Biol Chem 275(51):40504-10. [PubMed: 11001943]  [MGI Ref ID J:71007]

Additional References

Ephx2^tm1Gonz related

Luria A; Weldon SM; Kabcenell AK; Ingraham RH; Matera D; Jiang H; Gill R; Morisseau C; Newman JW; Hammock BD. 2007. Compensatory mechanism for homeostatic blood pressure regulation in Ephx2 gene-disrupted mice. J Biol Chem 282(5):2891-8. [PubMed: 17135253]  [MGI Ref ID J:120290]

Seubert JM; Sinal CJ; Graves J; DeGraff LM; Bradbury JA; Lee CR; Goralski K; Carey MA; Luria A; Newman JW; Hammock BD; Falck JR; Roberts H; Rockman HA; Murphy E; Zeldin DC. 2006. Role of soluble epoxide hydrolase in postischemic recovery of heart contractile function. Circ Res 99(4):442-50. [PubMed: 16857962]  [MGI Ref ID J:124860]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129X1 background and has been backcrossed to C57BL/6 for at least five generations.
Mating System	Homozygote x Homozygote         (Female x Male)

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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