Strain Name:

129-Itgb5tm1Des/J

Stock Number:

004166

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Dean Sheppard,   Univ of California Medical Center (UCSF)

Description
Mice that are homozygous for the Itgb5tm1Des targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No Itgb5 gene product (mRNA or protein) is detected. Homozygotes display defects in VEGF-mediated vascular permeability. Cultured keratinocytes derived from homozygous mutant animals display impaired adhesion and migration on vitronectin-coated surfaces.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Itgb5 gene corresponding to human amino acids 114-230. The construct was electroporated into 129S4/SvJae-derived RF8 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were mated to an unspecified strain of 129 mice.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Itgb5tm1Des/Itgb5tm1Des

        involves: 129S4/SvJae * C57BL/6J
  • normal phenotype
  • no abnormal phenotype detected
    • homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities   (MGI Ref ID J:59991)
  • integument phenotype
  • abnormal keratinocyte differentiation
    • cultured keratinocytes derived from homozygous animals display impaired adhesion and migration on vitronectin-coated surfaces   (MGI Ref ID J:59991)
  • cellular phenotype
  • abnormal keratinocyte differentiation
    • cultured keratinocytes derived from homozygous animals display impaired adhesion and migration on vitronectin-coated surfaces   (MGI Ref ID J:59991)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Internal/Organ Research
Skeleton
      Bone

Itgb5tm1Des related

Cancer Research
Growth Factors/Receptors/Cytokines
      Osteoporosis

Cardiovascular Research
Vascular Defects

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Vasculature Defects

Neurobiology Research

Research Tools
Cell Biology Research
Immunology, Inflammation and Autoimmunity Research
Internal/Organ Research

Virology Research
Adenovirus Receptor

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Itgb5tm1Des
Allele Name targeted mutation 1, Dean Sheppard
Allele Type Targeted (Null/Knockout)
Common Name(s) beta5-;
Mutation Made By Xiaozhu Huang,   Univ of California Medical Center (UCSF)
Strain of Origin129S4/SvJae
ES Cell Line NameRF8
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Itgb5, integrin beta 5
Chromosome 16
Gene Common Name(s) AA475909; AI874634; EST AA475909; ESTM23; RGD1563276; [b]-5; [b]5; [b]5A; [b]5B; beta-5; beta5; expressed sequence AI874634; expressed sequence tag mouse EST 23;
Molecular Note A neomycin resistance cassette replaced part of an exon of the gene that corresponds to amino acids 114 to 230 in the reported human sequence, and an adjacent intron. Northern analysis using a murine cDNA probe did not detect transcript in cultured keratinocytes from homozygous mutant mice. Immunoprecipitation studies using antiserum raised against the cytoplasmic domain of human did not detect protein in lysates of cultured keratinocytes from homozygous mutant mice. [MGI Ref ID J:59991]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Huang X; Griffiths M; Wu J; Farese RV Jr; Sheppard D. 2000. Normal development, wound healing, and adenovirus susceptibility in beta5-deficient mice. Mol Cell Biol 20(3):755-9. [PubMed: 10629031]  [MGI Ref ID J:59991]

Additional References

Reynolds LE; Wyder L; Lively JC; Taverna D; Robinson SD; Huang X; Sheppard D; Hynes RO; Hodivala-Dilke KM. 2002. Enhanced pathological angiogenesis in mice lacking beta3 integrin or beta3 and beta5 integrins. Nat Med 8(1):27-34. [PubMed: 11786903]  [MGI Ref ID J:73752]

Itgb5tm1Des related

Belvindrah R; Hankel S; Walker J; Patton BL; Muller U. 2007. Beta1 integrins control the formation of cell chains in the adult rostral migratory stream. J Neurosci 27(10):2704-17. [PubMed: 17344408]  [MGI Ref ID J:120076]

Di Paolo NC; Miao EA; Iwakura Y; Murali-Krishna K; Aderem A; Flavell RA; Papayannopoulou T; Shayakhmetov DM. 2009. Virus binding to a plasma membrane receptor triggers interleukin-1 alpha-mediated proinflammatory macrophage response in vivo. Immunity 31(1):110-21. [PubMed: 19576795]  [MGI Ref ID J:151628]

Henderson NC; Arnold TD; Katamura Y; Giacomini MM; Rodriguez JD; McCarty JH; Pellicoro A; Raschperger E; Betsholtz C; Ruminski PG; Griggs DW; Prinsen MJ; Maher JJ; Iredale JP; Lacy-Hulbert A; Adams RH; Sheppard D. 2013. Targeting of alphav integrin identifies a core molecular pathway that regulates fibrosis in several organs. Nat Med 19(12):1617-24. [PubMed: 24216753]  [MGI Ref ID J:205525]

Joly S; Samardzija M; Wenzel A; Thiersch M; Grimm C. 2009. Nonessential role of beta3 and beta5 integrin subunits for efficient clearance of cellular debris after light-induced photoreceptor degeneration. Invest Ophthalmol Vis Sci 50(3):1423-32. [PubMed: 18997092]  [MGI Ref ID J:146677]

Khalifeh-Soltani A; McKleroy W; Sakuma S; Cheung YY; Tharp K; Qiu Y; Turner SM; Chawla A; Stahl A; Atabai K. 2014. Mfge8 promotes obesity by mediating the uptake of dietary fats and serum fatty acids. Nat Med 20(2):175-83. [PubMed: 24441829]  [MGI Ref ID J:206863]

Lane NE; Yao W; Nakamura MC; Humphrey MB; Kimmel D; Huang X; Sheppard D; Ross FP; Teitelbaum SL. 2005. Mice lacking the integrin beta5 subunit have accelerated osteoclast maturation and increased activity in the estrogen-deficient state. J Bone Miner Res 20(1):58-66. [PubMed: 15619670]  [MGI Ref ID J:102247]

Milner R. 2009. Microglial expression of alphavbeta3 and alphavbeta5 integrins is regulated by cytokines and the extracellular matrix: beta5 integrin null microglia show no defects in adhesion or MMP-9 expression on vitronectin. Glia 57(7):714-23. [PubMed: 18985734]  [MGI Ref ID J:156216]

Nandrot EF; Anand M; Almeida D; Atabai K; Sheppard D; Finnemann SC. 2007. Essential role for MFG-E8 as ligand for {alpha}vbeta5 integrin in diurnal retinal phagocytosis. Proc Natl Acad Sci U S A 104(29):12005-10. [PubMed: 17620600]  [MGI Ref ID J:123148]

Nandrot EF; Anand M; Sircar M; Finnemann SC. 2006. Novel role for alphavbeta5-integrin in retinal adhesion and its diurnal peak. Am J Physiol Cell Physiol 290(4):C1256-62. [PubMed: 16338970]  [MGI Ref ID J:107356]

Nandrot EF; Kim Y; Brodie SE; Huang X; Sheppard D; Finnemann SC. 2004. Loss of synchronized retinal phagocytosis and age-related blindness in mice lacking alphavbeta5 integrin. J Exp Med 200(12):1539-45. [PubMed: 15596525]  [MGI Ref ID J:95338]

Reynolds LE; Wyder L; Lively JC; Taverna D; Robinson SD; Huang X; Sheppard D; Hynes RO; Hodivala-Dilke KM. 2002. Enhanced pathological angiogenesis in mice lacking beta3 integrin or beta3 and beta5 integrins. Nat Med 8(1):27-34. [PubMed: 11786903]  [MGI Ref ID J:73752]

Ruggiero L; Connor MP; Chen J; Langen R; Finnemann SC. 2012. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Proc Natl Acad Sci U S A 109(21):8145-8. [PubMed: 22566632]  [MGI Ref ID J:184772]

Schulz O; Pennington DJ; Hodivala-Dilke K; Febbraio M; Reis e Sousa C. 2002. CD36 or alphavbeta3 and alphavbeta5 integrins are not essential for MHC class I cross-presentation of cell-associated antigen by CD8 alpha+ murine dendritic cells. J Immunol 168(12):6057-65. [PubMed: 12055214]  [MGI Ref ID J:123797]

Su G; Hodnett M; Wu N; Atakilit A; Kosinski C; Godzich M; Huang XZ; Kim JK; Frank JA; Matthay MA; Sheppard D; Pittet JF. 2007. Integrin alphavbeta5 regulates lung vascular permeability and pulmonary endothelial barrier function. Am J Respir Cell Mol Biol 36(3):377-86. [PubMed: 17079779]  [MGI Ref ID J:132113]

Taverna D; Crowley D; Connolly M; Bronson RT; Hynes RO. 2005. A direct test of potential roles for beta3 and beta5 integrins in growth and metastasis of murine mammary carcinomas. Cancer Res 65(22):10324-9. [PubMed: 16288021]  [MGI Ref ID J:103405]

Yu CC; Nandrot EF; Dun Y; Finnemann SC. 2012. Dietary antioxidants prevent age-related retinal pigment epithelium actin damage and blindness in mice lacking alphavbeta5 integrin. Free Radic Biol Med 52(3):660-70. [PubMed: 22178979]  [MGI Ref ID J:180295]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated from a chimeric mouse on a B6;129S4 background which was mated to an unspecified 129 strain once before being made homozygous.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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