Description

Strain Information

Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation F?+4p (08-JAN-04)   Donating Investigator Dean Sheppard,   UCSF

Description
Mice that are homozygous for the Itgb5^tm1Des targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No Itgb5 gene product (mRNA or protein) is detected. Homozygotes display defects in VEGF-mediated vascular permeability. Cultured keratinocytes derived from homozygous mutant animals display impaired adhesion and migration on vitronectin-coated surfaces.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a region of the Itgb5 gene corresponding to human amino acids 114-230. The construct was electroporated into 129S4/SvJae-derived RF8 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were mated to an unspecified strain of 129 mice.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Itgb5^tm1Des/Itgb5^tm1Des

        involves: 129S4/SvJae * C57BL/6J

• skin/coat/nails phenotype
• abnormal keratinocyte differentiation (MGI Ref ID J:59991)
  • cultured keratinocytes derived from homozygous animals display impaired adhesion and migration on vitronectin-coated surfaces
• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:59991)
  • homozygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Itgb5^tm1Des related

Cancer Research
Growth Factors/Receptors/Cytokines (Osteoporosis)

Cardiovascular Research
Vascular Defects

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Vasculature Defects

Neurobiology Research

Research Tools
Cell Biology Research
Immunology and Inflammation Research
Internal/Organ Research

Virology Research
Adenovirus Receptor

Genes & Alleles

Gene & Allele Information

Allele Symbol		Itgb5tm1Des
Allele Name		targeted mutation 1, Dean Sheppard
Allele Type		Targeted (knock-out)
Common Name(s)		beta5-;
Mutation Made By		Xiaozhu Huang,   UCSF
Strain of Origin		129S4/SvJae
ES Cell Line Name		RF8
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Itgb5, integrin beta 5
Chromosome		16
Gene Common Name(s)		AA475909; AI874634; EST AA475909; ESTM23; FLJ26658; [b]-5; [b]5; [b]5A; [b]5B; beta-5; beta5; expressed sequence AI874634; expressed sequence tag mouse EST 23;
Molecular Note		Insertion of a neomycin resistance cassette replaced part of an exon of the gene that corresponds to amino acids 114 to 230 in the reported human sequence, and an adjacent intron. Northern analysis using a murine cDNA probe did not detect transcript in cultured keratinocytes from homozygous mutant mice. Immunoprecipitation studies using antiserum raised against the cytoplasmic domain of human did not detect protein in lysates of cultured keratinocytes from homozygous mutant mice. [MGI Ref ID J:59991]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Huang X; Griffiths M; Wu J; Farese RV Jr; Sheppard D. 2000. Normal development, wound healing, and adenovirus susceptibility in beta5-deficient mice. Mol Cell Biol 20(3):755-9. [PubMed: 10629031]  [MGI Ref ID J:59991]

Additional References

Reynolds LE; Wyder L; Lively JC; Taverna D; Robinson SD; Huang X; Sheppard D; Hynes RO; Hodivala-Dilke KM. 2002. Enhanced pathological angiogenesis in mice lacking beta3 integrin or beta3 and beta5 integrins. Nat Med 8(1):27-34. [PubMed: 11786903]  [MGI Ref ID J:73752]

Itgb5^tm1Des related

Belvindrah R; Hankel S; Walker J; Patton BL; Muller U. 2007. Beta1 integrins control the formation of cell chains in the adult rostral migratory stream. J Neurosci 27(10):2704-17. [PubMed: 17344408]  [MGI Ref ID J:120076]

Lane NE; Yao W; Nakamura MC; Humphrey MB; Kimmel D; Huang X; Sheppard D; Ross FP; Teitelbaum SL. 2005. Mice lacking the integrin beta5 subunit have accelerated osteoclast maturation and increased activity in the estrogen-deficient state. J Bone Miner Res 20(1):58-66. [PubMed: 15619670]  [MGI Ref ID J:102247]

Nandrot EF; Anand M; Almeida D; Atabai K; Sheppard D; Finnemann SC. 2007. Essential role for MFG-E8 as ligand for {alpha}vbeta5 integrin in diurnal retinal phagocytosis. Proc Natl Acad Sci U S A 104(29):12005-10. [PubMed: 17620600]  [MGI Ref ID J:123148]

Nandrot EF; Anand M; Sircar M; Finnemann SC. 2006. Novel role for alphavbeta5-integrin in retinal adhesion and its diurnal peak. Am J Physiol Cell Physiol 290(4):C1256-62. [PubMed: 16338970]  [MGI Ref ID J:107356]

Nandrot EF; Kim Y; Brodie SE; Huang X; Sheppard D; Finnemann SC. 2004. Loss of synchronized retinal phagocytosis and age-related blindness in mice lacking alphavbeta5 integrin. J Exp Med 200(12):1539-45. [PubMed: 15596525]  [MGI Ref ID J:95338]

Reynolds LE; Wyder L; Lively JC; Taverna D; Robinson SD; Huang X; Sheppard D; Hynes RO; Hodivala-Dilke KM. 2002. Enhanced pathological angiogenesis in mice lacking beta3 integrin or beta3 and beta5 integrins. Nat Med 8(1):27-34. [PubMed: 11786903]  [MGI Ref ID J:73752]

Schulz O; Pennington DJ; Hodivala-Dilke K; Febbraio M; Reis e Sousa C. 2002. CD36 or alphavbeta3 and alphavbeta5 integrins are not essential for MHC class I cross-presentation of cell-associated antigen by CD8 alpha+ murine dendritic cells. J Immunol 168(12):6057-65. [PubMed: 12055214]  [MGI Ref ID J:123797]

Su G; Hodnett M; Wu N; Atakilit A; Kosinski C; Godzich M; Huang XZ; Kim JK; Frank JA; Matthay MA; Sheppard D; Pittet JF. 2007. Integrin alphavbeta5 regulates lung vascular permeability and pulmonary endothelial barrier function. Am J Respir Cell Mol Biol 36(3):377-86. [PubMed: 17079779]  [MGI Ref ID J:132113]

Taverna D; Crowley D; Connolly M; Bronson RT; Hynes RO. 2005. A direct test of potential roles for beta3 and beta5 integrins in growth and metastasis of murine mammary carcinomas. Cancer Res 65(22):10324-9. [PubMed: 16288021]  [MGI Ref ID J:103405]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain originated from a chimeric mouse on a B6;129S4 background which was mated to an unspecified 129 strain once before being made homozygous.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.