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- Description
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Description
Strain Information
Former Names B6.Cg-Tg(CAG-Bgeo/GFP)21Lbe/J (Changed: 31-JUL-08 ) B6.Cg-Tg(ACTB-Bgeo/GFP)21Lbe/J (Changed: 08-MAY-08 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Generation N14 (11-DEC-07) Donating Investigator IMR Colony, The Jackson Laboratory Description
These Z/EG transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.As an example, when crossed to a strain expressing Cre recombinase in olfactory sensory neurons (see Stock No. 006668), this mutant mouse strain may be useful in lineage tracing.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
The Z/EG transgene was designed with the CMV enhancer/chicken beta-actin promoter, a loxP-flanked Bgeo reporter/SV40 polyadenylation signal segment, and an enhanced GFP gene followed by a rabbit beta-globin polyadenylation sequence. This transgene was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells expressing lacZ were selected and utilized in ES cell/diploid embryo aggregation in conjunction with CD1 outbred embryos resulting in chimeric animals. This strain was maintained by the donating investigator as a hemizygote by crosses to wildtype outbred CD1 mice for at least 3 generations prior to arrival at The Jackson Laboratory (as Stock No. 003920). Upon arrival, some mice were backcrossed to C57BL/6J for many generations to establish this congenic strain (Stock No. 004178).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (170 strains)
003920 STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Omptm4(cre)Mom/Omp+ Tg(CAG-Bgeo/GFP)21Lbe/0
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 (conditional)
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:115857)
- mice were used to generate permanently marked nuclear transfer ES cells and chimaeric or clonal mice
This mouse can be used to support research in many areas including:
Bgeo relatedNeurobiology Research
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Research Tools
lacZ Expression
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: multiple)
GFP relatedResearch Tools
lacZ Expression
Fluorescent ProteinslacZ related
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-Bgeo/GFP)21Lbe | ||
|---|---|---|---|
| Allele Name | transgene insertion 21, Corrinne Lobe | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(ACTB-Bgeo/GFP)21Lbe; Tg(CAG-Bgeo/GFP)21Lbe; Z/EG; Z/EG Cre; Z/EGLobe21; [ACTBBgeo/GFP] 21Lbe; | ||
| Mutation Made By | Corrinne Lobe, Sunnybrook & Women's College HSC | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being liver and lung; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with Enhanced Green Fluorescent Protein expression | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | Expression of lacZ is widespread in transgenic mice, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. | ||
| Molecular Note | This transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. [MGI Ref ID J:83045] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), MCA, vers. 2
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Novak A; Guo C; Yang W; Nagy A; Lobe CG. 2000. Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Genesis 28(3-4):147-55. [PubMed: 11105057] [MGI Ref ID J:83045]
Additional References
Tg(CAG-Bgeo/GFP)21Lbe relatedArmaka M; Apostolaki M; Jacques P; Kontoyiannis DL; Elewaut D; Kollias G. 2008. Mesenchymal cell targeting by TNF as a common pathogenic principle in chronic inflammatory joint and intestinal diseases. J Exp Med 205(2):331-7. [PubMed: 18250193] [MGI Ref ID J:131930]
Atouf F; Park CH; Pechhold K; Ta M; Choi Y; Lumelsky NL. 2007. No evidence for mouse pancreatic beta-cell epithelial-mesenchymal transition in vitro. Diabetes 56(3):699-702. [PubMed: 17327438] [MGI Ref ID J:122025]
Balthasar N; Coppari R; McMinn J; Liu SM; Lee CE; Tang V; Kenny CD; McGovern RA; Chua SC Jr; Elmquist JK; Lowell BB. 2004. Leptin receptor signaling in POMC neurons is required for normal body weight homeostasis. Neuron 42(6):983-91. [PubMed: 15207242] [MGI Ref ID J:106354]
Belvindrah R; Graus-Porta D; Goebbels S; Nave KA; Muller U. 2007. Beta1 integrins in radial glia but not in migrating neurons are essential for the formation of cell layers in the cerebral cortex. J Neurosci 27(50):13854-65. [PubMed: 18077697] [MGI Ref ID J:130562]
Breunig JJ; Silbereis J; Vaccarino FM; Sestan N; Rakic P. 2007. Notch regulates cell fate and dendrite morphology of newborn neurons in the postnatal dentate gyrus. Proc Natl Acad Sci U S A 104(51):20558-63. [PubMed: 18077357] [MGI Ref ID J:130582]
Bugeon L; Danou A; Carpentier D; Langridge P; Syed N; Dallman MJ. 2003. Inducible gene silencing in podocytes: a new tool for studying glomerular function. J Am Soc Nephrol 14(3):786-91. [PubMed: 12595517] [MGI Ref ID J:106338]
Carlen M; Meletis K; Barnabe-Heider F; Frisen J. 2006. Genetic visualization of neurogenesis. Exp Cell Res 312(15):2851-9. [PubMed: 16806169] [MGI Ref ID J:133759]
Chase LG; Ulloa-Montoya F; Kidder BL; Verfaillie CM. 2007. Islet-derived fibroblast-like cells are not derived via epithelial-mesenchymal transition from Pdx-1 or insulin-positive cells. Diabetes 56(1):3-7. [PubMed: 17110468] [MGI Ref ID J:121938]
Choudhury AI; Heffron H; Smith MA; Al-Qassab H; Xu AW; Selman C; Simmgen M; Clements M; Claret M; Maccoll G; Bedford DC; Hisadome K; Diakonov I; Moosajee V; Bell JD; Speakman JR; Batterham RL; Barsh GS; Ashford ML; Withers DJ. 2005. The role of insulin receptor substrate 2 in hypothalamic and beta cell function. J Clin Invest 115(4):940-950. [PubMed: 15841180] [MGI Ref ID J:97406]
Claret M; Smith MA; Batterham RL; Selman C; Choudhury AI; Fryer LG; Clements M; Al-Qassab H; Heffron H; Xu AW; Speakman JR; Barsh GS; Viollet B; Vaulont S; Ashford ML; Carling D; Withers DJ. 2007. AMPK is essential for energy homeostasis regulation and glucose sensing by POMC and AgRP neurons. J Clin Invest 117(8):2325-36. [PubMed: 17671657] [MGI Ref ID J:123951]
De Gasperi R; Rocher AB; Sosa MA; Wearne SL; Perez GM; Friedrich VL Jr; Hof PR; Elder GA. 2008. The IRG mouse: a two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ. Genesis 46(6):308-17. [PubMed: 18543298] [MGI Ref ID J:137251]
Delaunay D; Heydon K; Cumano A; Schwab MH; Thomas JL; Suter U; Nave KA; Zalc B; Spassky N. 2008. Early neuronal and glial fate restriction of embryonic neural stem cells. J Neurosci 28(10):2551-62. [PubMed: 18322099] [MGI Ref ID J:132754]
Demehri S; Liu Z; Lee J; Lin MH; Crosby SD; Roberts CJ; Grigsby PW; Miner JH; Farr AG; Kopan R. 2008. Notch-deficient skin induces a lethal systemic B-lymphoproliferative disorder by secreting TSLP, a sentinel for epidermal integrity. PLoS Biol 6(5):e123. [PubMed: 18507503] [MGI Ref ID J:139386]
Deutscher E; Hung-Chang Yao H. 2007. Essential roles of mesenchyme-derived beta-catenin in mouse Mullerian duct morphogenesis. Dev Biol 307(2):227-36. [PubMed: 17532316] [MGI Ref ID J:122979]
Dhillon H; Zigman JM; Ye C; Lee CE; McGovern RA; Tang V; Kenny CD; Christiansen LM; White RD; Edelstein EA; Coppari R; Balthasar N; Cowley MA; Chua S Jr; Elmquist JK; Lowell BB. 2006. Leptin directly activates SF1 neurons in the VMH, and this action by leptin is required for normal body-weight homeostasis. Neuron 49(2):191-203. [PubMed: 16423694] [MGI Ref ID J:105327]
Dimou L; Simon C; Kirchhoff F; Takebayashi H; Gotz M. 2008. Progeny of Olig2-expressing progenitors in the gray and white matter of the adult mouse cerebral cortex. J Neurosci 28(41):10434-42. [PubMed: 18842903] [MGI Ref ID J:141121]
Doyonnas R; LaBarge MA; Sacco A; Charlton C; Blau HM. 2004. Hematopoietic contribution to skeletal muscle regeneration by myelomonocytic precursors. Proc Natl Acad Sci U S A 101(37):13507-12. [PubMed: 15353585] [MGI Ref ID J:92609]
Dubois NC; Hofmann D; Kaloulis K; Bishop JM; Trumpp A. 2006. Nestin-Cre transgenic mouse line Nes-Cre1 mediates highly efficient Cre/loxP mediated recombination in the nervous system, kidney, and somite-derived tissues. Genesis 44(8):355-60. [PubMed: 16847871] [MGI Ref ID J:111945]
Earnheart JC; Schweizer C; Crestani F; Iwasato T; Itohara S; Mohler H; Luscher B. 2007. GABAergic control of adult hippocampal neurogenesis in relation to behavior indicative of trait anxiety and depression states. J Neurosci 27(14):3845-54. [PubMed: 17409249] [MGI Ref ID J:121218]
Engblom D; Bilbao A; Sanchis-Segura C; Dahan L; Perreau-Lenz S; Balland B; Parkitna JR; Lujan R; Halbout B; Mameli M; Parlato R; Sprengel R; Luscher C; Schutz G; Spanagel R. 2008. Glutamate receptors on dopamine neurons control the persistence of cocaine seeking. Neuron 59(3):497-508. [PubMed: 18701074] [MGI Ref ID J:139693]
Ferron M; Vacher J. 2005. Targeted expression of Cre recombinase in macrophages and osteoclasts in transgenic mice. Genesis 41(3):138-45. [PubMed: 15754380] [MGI Ref ID J:97319]
Furusho M; Ono K; Takebayashi H; Masahira N; Kagawa T; Ikeda K; Ikenaka K. 2006. Involvement of the Olig2 transcription factor in cholinergic neuron development of the basal forebrain. Dev Biol 293(2):348-57. [PubMed: 16537079] [MGI Ref ID J:108753]
Ganat YM; Silbereis J; Cave C; Ngu H; Anderson GM; Ohkubo Y; Ment LR; Vaccarino FM. 2006. Early postnatal astroglial cells produce multilineage precursors and neural stem cells in vivo. J Neurosci 26(33):8609-21. [PubMed: 16914687] [MGI Ref ID J:111657]
Gao X; Arlotta P; Macklis JD; Chen J. 2007. Conditional knock-out of beta-catenin in postnatal-born dentate gyrus granule neurons results in dendritic malformation. J Neurosci 27(52):14317-25. [PubMed: 18160639] [MGI Ref ID J:130955]
Garcia AD; Doan NB; Imura T; Bush TG; Sofroniew MV. 2004. GFAP-expressing progenitors are the principal source of constitutive neurogenesis in adult mouse forebrain. Nat Neurosci 7(11):1233-41. [PubMed: 15494728] [MGI Ref ID J:94543]
Haldar M; Karan G; Tvrdik P; Capecchi MR. 2008. Two cell lineages, myf5 and myf5-independent, participate in mouse skeletal myogenesis. Dev Cell 14(3):437-45. [PubMed: 18331721] [MGI Ref ID J:133338]
Hao Z; Guo C; Jiang X; Krueger S; Pietri T; Dufour S; Cone RE; O'Rourke J. 2006. New transgenic evidence for a system of sympathetic axons able to express tissue plasminogen activator (t-PA) within arterial/arteriolar walls. Blood 108(1):200-2. [PubMed: 16514060] [MGI Ref ID J:135574]
Hatori M; Le H; Vollmers C; Keding SR; Tanaka N; Schmedt C; Jegla T; Panda S. 2008. Inducible ablation of melanopsin-expressing retinal ganglion cells reveals their central role in non-image forming visual responses. PLoS ONE 3(6):e2451. [PubMed: 18545654] [MGI Ref ID J:137151]
Hauri-Hohl MM; Zuklys S; Keller MP; Jeker LT; Barthlott T; Moon AM; Roes J; Hollander GA. 2008. TGF-beta signaling in thymic epithelial cells regulates thymic involution and postirradiation reconstitution. Blood 112(3):626-34. [PubMed: 18474727] [MGI Ref ID J:138556]
Hayashi M; Imanaka-Yoshida K; Yoshida T; Wood M; Fearns C; Tatake RJ; Lee JD. 2006. A crucial role of mitochondrial Hsp40 in preventing dilated cardiomyopathy. Nat Med 12(1):128-32. [PubMed: 16327803] [MGI Ref ID J:104462]
He XC; Zhang J; Tong WG; Tawfik O; Ross J; Scoville DH; Tian Q; Zeng X; He X; Wiedemann LM; Mishina Y; Li L. 2004. BMP signaling inhibits intestinal stem cell self-renewal through suppression of Wnt-beta-catenin signaling. Nat Genet 36(10):1117-21. [PubMed: 15378062] [MGI Ref ID J:93850]
Herrmann JE; Imura T; Song B; Qi J; Ao Y; Nguyen TK; Korsak RA; Takeda K; Akira S; Sofroniew MV. 2008. STAT3 is a critical regulator of astrogliosis and scar formation after spinal cord injury. J Neurosci 28(28):7231-43. [PubMed: 18614693] [MGI Ref ID J:138589]
High FA; Zhang M; Proweller A; Tu L; Parmacek MS; Pear WS; Epstein JA. 2007. An essential role for Notch in neural crest during cardiovascular development and smooth muscle differentiation. J Clin Invest 117(2):353-363. [PubMed: 17273555] [MGI Ref ID J:118039]
Hoogaars WM; Engel A; Brons JF; Verkerk AO; de Lange FJ; Wong LY; Bakker ML; Clout DE; Wakker V; Barnett P; Ravesloot JH; Moorman AF; Verheijck EE; Christoffels VM. 2007. Tbx3 controls the sinoatrial node gene program and imposes pacemaker function on the atria. Genes Dev 21(9):1098-112. [PubMed: 17473172] [MGI Ref ID J:121230]
Janoschek R; Plum L; Koch L; Munzberg H; Diano S; Shanabrough M; Muller W; Horvath TL; Bruning JC. 2006. gp130 signaling in proopiomelanocortin neurons mediates the acute anorectic response to centrally applied ciliary neurotrophic factor. Proc Natl Acad Sci U S A 103(28):10707-12. [PubMed: 16818888] [MGI Ref ID J:111814]
Jayasena CS; Ohyama T; Segil N; Groves AK. 2008. Notch signaling augments the canonical Wnt pathway to specify the size of the otic placode. Development 135(13):2251-61. [PubMed: 18495817] [MGI Ref ID J:137096]
Jullien N; Goddard I; Selmi-Ruby S; Fina JL; Cremer H; Herman JP. 2007. Conditional Transgenesis Using Dimerizable Cre (DiCre). PLoS ONE 2(12):e1355. [PubMed: 18159238] [MGI Ref ID J:130959]
Kang TB; Ben-Moshe T; Varfolomeev EE; Pewzner-Jung Y; Yogev N; Jurewicz A; Waisman A; Brenner O; Haffner R; Gustafsson E; Ramakrishnan P; Lapidot T; Wallach D. 2004. Caspase-8 serves both apoptotic and nonapoptotic roles. J Immunol 173(5):2976-84. [PubMed: 15322156] [MGI Ref ID J:92722]
Kido T; Lau YF. 2005. A Cre gene directed by a human TSPY promoter is specific for germ cells and neurons. Genesis 42(4):263-75. [PubMed: 16035036] [MGI Ref ID J:101097]
Lammert E; Gu G; McLaughlin M; Brown D; Brekken R; Murtaugh LC; Gerber HP; Ferrara N; Melton DA. 2003. Role of VEGF-A in vascularization of pancreatic islets. Curr Biol 13(12):1070-4. [PubMed: 12814555] [MGI Ref ID J:128154]
Lang D; Lu MM; Huang L; Engleka KA; Zhang M; Chu EY; Lipner S; Skoultchi A; Millar SE; Epstein JA. 2005. Pax3 functions at a nodal point in melanocyte stem cell differentiation. Nature 433(7028):884-7. [PubMed: 15729346] [MGI Ref ID J:96431]
Lee N; Robitz R; Zurbrugg RJ; Karpman AM; Mahler AM; Cronier SA; Vesey R; Spearry RP; Zolotukhin S; Maclennan AJ. 2008. Conditional, genetic disruption of ciliary neurotrophic factor receptors reveals a role in adult motor neuron survival. Eur J Neurosci 27(11):2830-7. [PubMed: 18588528] [MGI Ref ID J:137159]
Li G; Adesnik H; Li J; Long J; Nicoll RA; Rubenstein JL; Pleasure SJ. 2008. Regional distribution of cortical interneurons and development of inhibitory tone are regulated by Cxcl12/Cxcr4 signaling. J Neurosci 28(5):1085-98. [PubMed: 18234887] [MGI Ref ID J:131835]
Li J; Ishii T; Feinstein P; Mombaerts P. 2004. Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons. Nature 428(6981):393-9. [PubMed: 15042081] [MGI Ref ID J:115857]
Lush ME; Li Y; Kwon CH; Chen J; Parada LF. 2008. Neurofibromin is required for barrel formation in the mouse somatosensory cortex. J Neurosci 28(7):1580-7. [PubMed: 18272679] [MGI Ref ID J:132220]
Masahira N; Takebayashi H; Ono K; Watanabe K; Ding L; Furusho M; Ogawa Y; Nabeshima Y; Alvarez-Buylla A; Shimizu K; Ikenaka K. 2006. Olig2-positive progenitors in the embryonic spinal cord give rise not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells. Dev Biol 293(2):358-69. [PubMed: 16581057] [MGI Ref ID J:108719]
Mason HA; Rakowiecki SM; Raftopoulou M; Nery S; Huang Y; Gridley T; Fishell G. 2005. Notch signaling coordinates the patterning of striatal compartments. Development 132(19):4247-58. [PubMed: 16120638] [MGI Ref ID J:101735]
Meletis K; Barnabe-Heider F; Carlen M; Evergren E; Tomilin N; Shupliakov O; Frisen J. 2008. Spinal cord injury reveals multilineage differentiation of ependymal cells. PLoS Biol 6(7):e182. [PubMed: 18651793] [MGI Ref ID J:139534]
Monory K; Massa F; Egertova M; Eder M; Blaudzun H; Westenbroek R; Kelsch W; Jacob W; Marsch R; Ekker M; Long J; Rubenstein JL; Goebbels S; Nave KA; During M; Klugmann M; Wolfel B; Dodt HU; Zieglgansberger W; Wotjak CT; Mackie K; Elphick MR; Marsicano G; Lutz B. 2006. The endocannabinoid system controls key epileptogenic circuits in the hippocampus. Neuron 51(4):455-66. [PubMed: 16908411] [MGI Ref ID J:122066]
Murdoch B; Roskams AJ. 2008. A novel embryonic nestin-expressing radial glia-like progenitor gives rise to zonally restricted olfactory and vomeronasal neurons. J Neurosci 28(16):4271-82. [PubMed: 18417707] [MGI Ref ID J:134542]
Nakhai H; Sel S; Favor J; Mendoza-Torres L; Paulsen F; Duncker GI; Schmid RM. 2007. Ptf1a is essential for the differentiation of GABAergic and glycinergic amacrine cells and horizontal cells in the mouse retina. Development 134(6):1151-60. [PubMed: 17301087] [MGI Ref ID J:119184]
Ohyama T; Mohamed OA; Taketo MM; Dufort D; Groves AK. 2006. Wnt signals mediate a fate decision between otic placode and epidermis. Development 133(5):865-75. [PubMed: 16452098] [MGI Ref ID J:105983]
Ono K; Takebayashi H; Ikeda K; Furusho M; Nishizawa T; Watanabe K; Ikenaka K. 2008. Regional- and temporal-dependent changes in the differentiation of Olig2 progenitors in the forebrain, and the impact on astrocyte development in the dorsal pallium. Dev Biol 320(2):456-68. [PubMed: 18582453] [MGI Ref ID J:139148]
Perl AK; Wert SE; Loudy DE; Shan Z; Blair PA; Whitsett JA. 2005. Conditional recombination reveals distinct subsets of epithelial cells in trachea, bronchi, and alveoli. Am J Respir Cell Mol Biol 33(5):455-62. [PubMed: 16055670] [MGI Ref ID J:132772]
Plum L; Rother E; Munzberg H; Wunderlich FT; Morgan DA; Hampel B; Shanabrough M; Janoschek R; Konner AC; Alber J; Suzuki A; Krone W; Horvath TL; Rahmouni K; Bruning JC. 2007. Enhanced leptin-stimulated pi3k activation in the CNS promotes white adipose tissue transdifferentiation. Cell Metab 6(6):431-45. [PubMed: 18054313] [MGI Ref ID J:130444]
Schuller U; Heine VM; Mao J; Kho AT; Dillon AK; Han YG; Huillard E; Sun T; Ligon AH; Qian Y; Ma Q; Alvarez-Buylla A; McMahon AP; Rowitch DH; Ligon KL. 2008. Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form Shh-induced medulloblastoma. Cancer Cell 14(2):123-34. [PubMed: 18691547] [MGI Ref ID J:139574]
Song N; Schwab KR; Patterson LT; Yamaguchi T; Lin X; Potter SS; Lang RA. 2007. pygopus 2 has a crucial, Wnt pathway-independent function in lens induction. Development 134(10):1873-85. [PubMed: 17428831] [MGI Ref ID J:121416]
Spassky N; Han YG; Aguilar A; Strehl L; Besse L; Laclef C; Ros MR; Garcia-Verdugo JM; Alvarez-Buylla A. 2008. Primary cilia are required for cerebellar development and Shh-dependent expansion of progenitor pool. Dev Biol 317(1):246-59. [PubMed: 18353302] [MGI Ref ID J:136079]
Stacy RC; Demas J; Burgess RW; Sanes JR; Wong RO. 2005. Disruption and recovery of patterned retinal activity in the absence of acetylcholine. J Neurosci 25(41):9347-57. [PubMed: 16221843] [MGI Ref ID J:101622]
Stanger BZ; Stiles B; Lauwers GY; Bardeesy N; Mendoza M; Wang Y; Greenwood A; Cheng KH; McLaughlin M; Brown D; Depinho RA; Wu H; Melton DA; Dor Y. 2005. Pten constrains centroacinar cell expansion and malignant transformation in the pancreas. Cancer Cell 8(3):185-95. [PubMed: 16169464] [MGI Ref ID J:102226]
Stoller JZ; Degenhardt KR; Huang L; Zhou DD; Lu MM; Epstein JA. 2008. Cre reporter mouse expressing a nuclear localized fusion of GFP and beta-galactosidase reveals new derivatives of Pax3-expressing precursors. Genesis 46(4):200-4. [PubMed: 18395835] [MGI Ref ID J:135149]
Tong Q; Ye C; McCrimmon RJ; Dhillon H; Choi B; Kramer MD; Yu J; Yang Z; Christiansen LM; Lee CE; Choi CS; Zigman JM; Shulman GI; Sherwin RS; Elmquist JK; Lowell BB. 2007. Synaptic glutamate release by ventromedial hypothalamic neurons is part of the neurocircuitry that prevents hypoglycemia. Cell Metab 5(5):383-93. [PubMed: 17488640] [MGI Ref ID J:129860]
Tong Q; Ye CP; Jones JE; Elmquist JK; Lowell BB. 2008. Synaptic release of GABA by AgRP neurons is required for normal regulation of energy balance. Nat Neurosci :. [PubMed: 18690230] [MGI Ref ID J:140858]
Weissgerber P; Held B; Bloch W; Kaestner L; Chien KR; Fleischmann BK; Lipp P; Flockerzi V; Freichel M. 2006. Reduced cardiac L-type Ca2+ current in Ca(V)beta2-/- embryos impairs cardiac development and contraction with secondary defects in vascular maturation. Circ Res 99(7):749-57. [PubMed: 16946137] [MGI Ref ID J:126506]
Wen PH; De Gasperi R; Sosa MA; Rocher AB; Friedrich VL Jr; Hof PR; Elder GA. 2005. Selective expression of presenilin 1 in neural progenitor cells rescues the cerebral hemorrhages and cortical lamination defects in presenilin 1-null mutant mice. Development 132(17):3873-83. [PubMed: 16079160] [MGI Ref ID J:100132]
Willaime-Morawek S; Seaberg RM; Batista C; Labbe E; Attisano L; Gorski JA; Jones KR; Kam A; Morshead CM; van der Kooy D. 2006. Embryonic cortical neural stem cells migrate ventrally and persist as postnatal striatal stem cells. J Cell Biol 175(1):159-68. [PubMed: 17030986] [MGI Ref ID J:114513]
Williams CJ; Naito T; Arco PG; Seavitt JR; Cashman SM; De Souza B; Qi X; Keables P; Von Andrian UH; Georgopoulos K. 2004. The chromatin remodeler Mi-2beta is required for CD4 expression and T cell development. Immunity 20(6):719-33. [PubMed: 15189737] [MGI Ref ID J:109547]
Xu Q; Tam M; Anderson SA. 2008. Fate mapping Nkx2.1-lineage cells in the mouse telencephalon. J Comp Neurol 506(1):16-29. [PubMed: 17990269] [MGI Ref ID J:131144]
Xu S; Sunderland ME; Coles BL; Kam A; Holowacz T; Ashery-Padan R; Marquardt T; McInnes RR; van der Kooy D. 2007. The proliferation and expansion of retinal stem cells require functional Pax6. Dev Biol 304(2):713-21. [PubMed: 17316600] [MGI Ref ID J:122517]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these Z/EG transgenic mice are bred to C57BL/6J or wildtype littermates. Compared to transgenic mice on a mixed background, mutants on this background may experience increased incidence of reduced offspring size and in utero and neonatal mortality. The majority of the lethality is postnatal with only a 10% loss of hemizygous mice by 2 weeks of age. Survival of pups is increased by delayed weaning and pups that survive will breed. Note: This transgenic line is available from The Jackson Laboratory on a STOCK background (Stock No. 003920), which is not subject to problems with postnatal mortality in a hemizygous state; homozygotes were not viable. This colony is currently being maintained by breeding hemizygotes with C57BL/6J (and reciprocal).
Mating System Inbred x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(CAG-Bgeo/GFP)21Lbe *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $253.30 C57BL/6J (000664) x Hemizygous for Tg(CAG-Bgeo/GFP)21Lbe $251.90 Hemizygous for Tg(CAG-Bgeo/GFP)21Lbe x C57BL/6J (000664)
Additional Supply Details
| Supply Notes |
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|---|
| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(CAG-Bgeo/GFP)21Lbe
Pairs /Price* Pair Genotype $329.30 C57BL/6J (000664) x Hemizygous for Tg(CAG-Bgeo/GFP)21Lbe $327.50 Hemizygous for Tg(CAG-Bgeo/GFP)21Lbe x C57BL/6J (000664)