Strain Name: |
B6.Cg-Tg(CAG-Bgeo/GFP)21Lbe/J |
|---|---|
Stock Number: |
004178 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(ACTB-Bgeo/GFP)21Lbe/J (Changed: 08-MAY-08
) |
| Genes & Alleles | ACTB; Bgeo; GFP; lacZ; Tg(CAG-Bgeo/GFP)21Lbe; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N14 (11-DEC-07) Strain Description
These transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread with notable exceptions being liver and lung tissue. Expression is observed throughout all embryonic and adult stages. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with enhanced GFP expression in tissues expressing Cre. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells.As an example, when crossed to a strain expressing Cre recombinase in olfactory sensory neurons (see Stock No. 006668), this mutant mouse strain may be useful in lineage tracing.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing the CMV enhancer/chicken beta-actin promoter, a loxP-flanked Bgeo reporter/SV40 polyadenylation signal segment, and an enhanced GFP gene followed by a rabbit beta-globin polyadenylation sequence, was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. ES cells expressing lacZ were selected and utilized in ES cell/diploid embryo aggregation in conjunction with CD1 outbred embryos resulting in chimeric animals. This strain was maintained by the donating investigator as a hemizygote by crosses to wildtype outbred CD1 mice. It was backcrossed to C57BL/6J (N7) at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(CAG-Bgeo/GFP)21Lbe | ||
|---|---|---|---|
| Allele Name | transgene insertion 21, Corrinne Lobe | ||
| Common Name(s) | Tg(ACTB-Bgeo/GFP)21Lbe; Z/EG; Z/EG Cre; [ACTBBgeo/GFP] 21Lbe; | ||
| Mutation Made By | Corrinne Lobe, Sunnybrook & Women's College HSC | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being liver and lung; when crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with Enhanced Green Fluorescent Protein expression | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | Expression of lacZ is widespread in transgenic mice, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. | ||
| Molecular Note | This transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. [J:83045] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice are bred to C57BL/6J or wildtype littermates. Compared to transgenic mice on a mixed background, mutants on this background may experience increased incidence of reduced offspring size and in utero and neonatal mortality. The majority of the lethality is postnatal with only a 10% loss of hemizygous mice by 2 weeks of age. Survival of pups is increased by delayed weaning and pups that survive will breed. Note: This transgenic line is available from The Jackson Laboratory on a STOCK background (Stock No. 003920), which is not subject to problems with postnatal mortality in a hemizygous state; homozygotes were not viable. This colony is currently being maintained by breeding hemizygotes with C57BL/6J (and reciprocal). |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
003920 STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Bgeo relatedResearch Tools
Cre-lox-System (loxP-flanked Sequences: Test/Reporter)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Genetics Research (Tissue/Cell Markers: multiple)
lacZ Expression
GFP relatedResearch Tools
lacZ Expression
Fluorescent ProteinslacZ related
lacZ Expression
References
Selected Reference(s)
Additional ReferencesNovak A; Guo C; Yang W; Nagy A; Lobe CG. 2000. Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Genesis 28(3-4):147-55. [PubMed: 11105057] [J:83045]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(CAG-Bgeo/GFP)21Lbe/J |
| Stock Number: | 004178 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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