Description

Strain Information

Former Names B6;FVB Tg(Wap-HRAS)69Lln Chr YSJL-Edaraddcr-3J/J    (Changed: 15-DEC-04 ) B6;FVB Tg(WapHRAS)69Lln YSJL-Edaraddcr-3J/J    (Changed: 15-DEC-04 ) B6;FVB-TgN(WapHRAS)69Lln YSJL-cr-3J    (Changed: 15-DEC-04 ) B6;FVB-TgN(WapHRAS)69Lln YSJL-Edaraddcr-3J    (Changed: 15-DEC-04 ) BB6;FVB Tg(WapHRAS)69Lln Chr YSJL-Edaraddcr-3J/J    (Changed: 15-DEC-04 ) Type Consomic; Additional information on Consomic Mice. Type Mutant Stock; Spontaneous Mutation; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N3

Development
Mice exhibitiing the crinkled 3 Jackson (Edaradd^cr-3J) phenotype were found in 2000 by Harold Coombs in Dr. Wesley Beamer's colony of FVB/N-Tg(WapHRAS)69Lln Chr Y^SJL/J mice at The Jackson Laboratory. Edaradd^cr-3J homozygous males were crossed to C57BL/6J females; these pairs and their F1 progeny were donated to Dr. Leah Rae Donahue in the Mouse Mutant Resource. Homozygous F2 male progeny of these crosses were again crossed to C57BL/6J females, and sperm from N2F2 males was cryopreserved. Mice recovered by in vitro fertilization using the frozen sperm and ova from C57BL/6J females will thus be at backcross generation N3 to C57BL/6J.

The background strain on which Edaradd^cr-3J arose was then at backcross generation N14 to FVB/N. It carried, and still carries, a transgene comprising the human Harvey rat sarcoma (HRAS) oncogene homolog under control of the whey acidic protein promoter. The transgene was originally injected into a B6SJLF1 zygote, and it integrated into the Y Chromosome. It was transferred onto and maintained on the FVB/N background by crossing male mice hemizygous for the transgene with wild-type FVB/N female mice for 14 generations, after which it was maintained by crossing hemizygous males with their sisters. Male mice with this transgene on on a segregating B6;SJL background develop multiple tumors of the mammary and salivary glands by 1 year of age, and on the FVB/N background they develop multiple mammary tumors by age 6-8 weeks; the tumor phenotype has not been characterized on the B6;FVB segregating background, but some males of the Edaradd^cr-3J bearing stock developed visible mammary gland tumors and became moribund between 4 and 8 months of age (LR Donahue, personal communication). The transgene can be eliminated from the stock by crossing a female homozygous for Edaradd^cr-3J with a wild-type male mouse, which will replace the transgene-bearing Y Chromosome with that of the wild-type strain.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Edaradd^cr-3J/Edaradd^cr-3J

        FVB-Tg(Wap-HRAS)69Lln Chr Y^SJL

• limbs/digits/tail phenotype
• abnormal tail morphology (MGI Ref ID J:87623)
  • mice exhibit a tiny fork or kink in the tail near the tip
• reproductive system phenotype
• reduced fertility (MGI Ref ID J:87623)
  • both male and female homozygotes are fertile, but they are not reliable breeders
• respiratory system phenotype
• abnormal respiratory sounds (MGI Ref ID J:87623)
  • a respiratory disorder indicated by a chirping sound
• skin/coat/nails phenotype
• sparse hair (MGI Ref ID J:87623)
  • mice have sparse fur on the belly and bald patches behind ears

Genes & Alleles

Gene & Allele Information

Allele Symbol		Edaraddcr-3J
Allele Name		crinkled 3 Jackson
Allele Type		Spontaneous
Strain of Origin		FVB.Cg-Tg(Wap-HRAS)69Lln Chr YSJL
Gene Symbol and Name		Edaradd, EDAR (ectodysplasin-A receptor)-associated death domain
Chromosome		13
Gene Common Name(s)		1810032E07Rik; 5830469M23Rik; ED3; EDA3; RGD1564010; RIKEN cDNA 1810032E07 gene; RIKEN cDNA 5830469M23 gene; cr; crinkled;
General Note		Extensive characterization has been done for the original crinkled mutation (cr), but comparable data are unavailable for this remutation.
Molecular Note		This spontaneous mutant was identified as an remutation at the Edaradd locus through complementation analysis with B6C3Fe-a/a-Edaraddcr.
Gene Symbol and Name		Tg(Wap-HRAS)69Lln, transgene insertion 69, Loretta L Nielsen
Chromosome		Y
Gene Common Name(s)		Tg(Wap-HRAS)69Lin; Tg(WapHRAS)69Lin;

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Edaradd^cr-3J related

Donahue LR; Marden CC; Coombs H. 2004. Edaradd, a remutation to crinkled MGI Direct Data Submission :.  [MGI Ref ID J:87623]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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