Strain Name: |
C.129-Il4tm1Lky/J |
|---|---|
Stock Number: |
004190 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Il4; Il4tm1Lky; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Richard Locksley, Univ of California San Francisco-HHMI Generation N5F?+F12N1F0 (06-DEC-07) Strain Description
Mice homozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A knockin replaces the endogenous gene with an Il4/IRES/EGFP gene (IL4 activity remains intact). This leads to generation of a bicistronic transcript under the control of endogenous regulatory elements. Cells activated to express IL4 will express EGFP, allowing reliable in vivo tracking of both innate and adaptive immune cells, and/or enabling their isolation without further stimulation. As the IRES element promotes translational competence capable of revealing low-level transcription otherwise not apparent from the canonical 5'-cap of the mRNA, cells from these mice can also be used to report competence for IL4 production upon stimulation.Strain Development
A targeting vector containing herpes simplex virus thymidine kinase, Il4 exon 3-4 sequence, a loxP-flanked neomycin cassette, and an IRES-EGFP construct was utilized in the making of this mutant. The vector was electroporated into 129T2 X 129S4-derived PrmCre embryonic stem (ES) cells. These ES cells express Cre recombinase under the direction of the mouse protamine 1 promoter (expresses in testis/male germline). Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to BALB/cAnNCrlBR mice.
Gene & Allele Details
| Allele Symbol | Il4tm1Lky | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard M Locksley | ||
| Common Name(s) | 4 get; 4get; | ||
| Mutation Made By | Markus Mohrs, Trudeau Institute, Inc. | ||
| Site of Expression | Cells activated to express IL-4 will express GFP. IL-4 activity remains intact. | ||
| Gene Symbol and Name | Il4, interleukin 4 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | BCGF-1; BCGF1; BSF1; IL-4; Il-4; Il4e12; MGC79402; | ||
| Molecular Note | A loxP-flanked neomycin cassette followed by an internal ribosomal entry site (IRES) and an EGFP gene was inserted into sequences corresponding to the 3' untranslated region in exon 4. The neomycin cassette was removed by Cre mediated germline recombination in chimeric mice to generate the final allele. These mice express green fluorescent protein under the control of the Il4 promoter. [J:75415] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Il4tm1Lky | None Available | |
| None Available | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
IL4/GFPtm1Lky
Il4tm1Lky
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
006698 NOD.Cg-Il4tm1Lky/JbsJ
002253 B6.129P2-Il4tm1Cgn/J 002496 BALB/c-Il4tm2Nnt/J 003480 C.129S2(B6)-Il4tm1Gru/J 002518 C57BL/6-Il4tm1Nnt/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J 005879 D1Lac.Cg-Il4tm1Cgn/J 002574 NOD.129P2(B6)-Il4tm1Cgn/Dvs 004222 NOD.129P2(B6)-Il4tm1Cgn/DvsJ 004228 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/Dvs 004291 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX Notes, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Il4tm1Lky relatedImmunology and Inflammation Research
Intracellular Signaling Molecules
Research Tools
Fluorescent Proteins
Immunology and Inflammation Research
Cancer Research
Growth Factors/Receptors/Cytokines
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
References
Selected Reference(s)
Additional ReferencesMohrs M; Shinkai K; Mohrs K; Locksley RM. 2001. Analysis of type 2 immunity in vivo with a bicistronic IL-4 reporter. Immunity 15(2):303-11. [PubMed: 11520464] [J:75415]
Price and Supply Information
| Strain Name: | C.129-Il4tm1Lky/J |
| Stock Number: | 004190 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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