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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg(CB)-Tg(HLA-A/H2-D)2Enge/J (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Hemizygote x Hemizygote (Female x Male) Species laboratory mouse Generation NE11F9 (31-DEC-07) Donating Investigator Victor Engelhard, University of Virginia Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express an interspecies hybrid class I MHC gene, AAD, which contains the alpha-1 and alpha-2 domains of the human HLA-A2.1 gene and the alpha-3 transmembrane and cytoplasmic domains of the mouse H-2Dd gene, under the direction of the human HLA-A2.1 promoter. Immunodetection of the HLA-A2.1 recombinant transgene established that expression was at equivalent levels to endogenous mouse class I molecules. The mouse alpha-3 domain expression enhances the immune response in this system. Compared to unmodified HLA-A2.1, the chimeric HLA-A2.1/H2-Dd MHC Class I molecule mediates efficient positive selection of mouse T cells to provide a more complete T cell repertoire capable of recognizing peptides presented by HLA-A2.1 Class I molecules. The peptide epitopes presented and recognized by mouse T cells in the context of the HLA-A2.1/H2-Dd class I molecule are the same as those presented in HLA-A2.1+ humans. This transgenic strain enables the modeling of human T cell immune responses to HLA-A2 presented antigens, and identification of those antigens. This transgenic strain is an important preclinical model for design and testing of vaccines for infectious diseases or cancer therapy involving optimal stimulation of Cd8+ cytolytic T cells.Development
A transgenic construct containing the leader, alpha-1 and alpha-2 domains of human HLA-A2.1 and the alpha-3 transmembrane and cytoplasmic domains of mouse H-2Dd was injected into fertilized (C57BL/6 X CBA/Ca)F1 mouse eggs. Founder animals were bred with C57BL/6 mice.
Control Information
| Control | ||
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| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of HLA-A
View Strains carrying other alleles of HLA-A (9 strains)
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(HLA-A/H2-D)2Enge/?
(C57BL/6 x CBA)F1
- immune system phenotype
- abnormal cytotoxic T cell physiology (MGI Ref ID J:31937)
- CD8 T cells from influenza infected transgenic mice lyse target cells presenting influenza peptide on either human HLA-A2.1 (MHC-I) or chimeric human mouse HLA-A/H2-D (MHC-I) complexes
- CD8 T cells lyse target cells expressing the chimeric MHC-I complex with 10-fold higher efficiency compared to target cell expressing the human MHC-I complex
- abnormal negative T cell selection (MGI Ref ID J:31937)
- T cells that recognize the human MHC-1 complex HLA-A2.1 are not negatively selected
- abnormal positive T cell selection (MGI Ref ID J:31937)
- positive selection of T cells capable of responding to antigen presented by chimeric MHC-1 complexes is enhanced compared to selection in Tg(HLA-A2.1)1Enge mice that express human MHC-1
- the chimeric MHC Class I molecule mediates efficient positive selection of mouse T cells to provide a more complete T cell repertoire capable of recognizing peptides presented by HLA-A2.1 Class I molecules.
- The peptide epitopes presented and recognized by mouse T cells in the context of the HLA-A2.1/H2-Dd class I molecule are the same as those presented in HLA-A2.1+ humans.
- hematopoietic system phenotype
- abnormal negative T cell selection (MGI Ref ID J:31937)
- T cells that recognize the human MHC-1 complex HLA-A2.1 are not negatively selected
- abnormal positive T cell selection (MGI Ref ID J:31937)
- positive selection of T cells capable of responding to antigen presented by chimeric MHC-1 complexes is enhanced compared to selection in Tg(HLA-A2.1)1Enge mice that express human MHC-1
- the chimeric MHC Class I molecule mediates efficient positive selection of mouse T cells to provide a more complete T cell repertoire capable of recognizing peptides presented by HLA-A2.1 Class I molecules.
- The peptide epitopes presented and recognized by mouse T cells in the context of the HLA-A2.1/H2-Dd class I molecule are the same as those presented in HLA-A2.1+ humans.
This mouse can be used to support research in many areas including:
HLA-A relatedResearch Tools
Cancer Research (production of B and T cells, antibodies, and hybridomas)
Cancer Research (production of T cells and hybridoma)
Cancer Research (tumor immunology)
Cancer Research (xenograft/transplant host)
Developmental Biology Research (transplantation marker for embryonic and adult tissue)
Genetics Research (Tissue/Cell Markers: cell marker for bone marrow transplantation)
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Vaccine Development
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(HLA-A/H2-D)2Enge | ||
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| Allele Name | transgene insertion 2, Victor H Engelhard | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | AAD mice; | ||
| Mutation Made By | Victor Engelhard, University of Virginia | ||
| Strain of Origin | (C57BL/6 x CBA/Ca)F1 | ||
| Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human | ||
| Molecular Note | Transgenic mice express an interspecies hybrid class I MHC gene, AAD, which contains the alpha-1 and alpha-2 domains of the human HLA-A2.1 gene and the alpha-3 transmembrane and cytoplasmic domains of the murine H-2D gene, under the direction of the human HLA-A2.1 promoter. Immunodetection of the HLA-A2.1 recombinant transgene established that expression was at equivalent levels to endogenous murine class I molecules and to similar transgenic strains. [MGI Ref ID J:31937] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(HLA-A/H2-D), QPCR, vers. 2
Tg(HLA-A/H2-D), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Newberg MH; Smith DH; Haertel SB; Vining DR; Lacy E; Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156(7):2473-80. [PubMed: 8786307] [MGI Ref ID J:31937]
Additional References
Tg(HLA-A/H2-D)2Enge relatedLengagne R; Graff-Dubois S; Garcette M; Renia L; Kato M; Guillet JG; Engelhard VH; Avril MF; Abastado JP; Prevost-Blondel A. 2008. Distinct role for CD8 T cells toward cutaneous tumors and visceral metastases. J Immunol 180(1):130-7. [PubMed: 18097012] [MGI Ref ID J:130879]
Qian J; Dong Y; Pang YY; Ibrahim R; Berzofsky JA; Schiller JT; Khleif SN. 2006. Combined prophylactic and therapeutic cancer vaccine: enhancing CTL responses to HPV16 E2 using a chimeric VLP in HLA-A2 mice. Int J Cancer 118(12):3022-9. [PubMed: 16425257] [MGI Ref ID J:112324]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry Founder mice were backcrossed to C57BL/6 animals for at least 10 generation before being made homozygous. Mating System Hemizygote x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(HLA-A/H2-D)2Enge $236.40 Female or Male Homozygous for Tg(HLA-A/H2-D)2Enge *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Hemizygous for Tg(HLA-A/H2-D)2Enge x Hemizygous for Tg(HLA-A/H2-D)2Enge
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(HLA-A/H2-D)2Enge $307.40 Female or Male Homozygous for Tg(HLA-A/H2-D)2Enge *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Hemizygous for Tg(HLA-A/H2-D)2Enge x Hemizygous for Tg(HLA-A/H2-D)2Enge
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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