Strain Name: |
STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J |
|---|---|
Stock Number: |
004192 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy TgN(Mx1-cre)1Cgn (Changed: 15-DEC-04
) |
|
STOCK-Mttptm1SgyLdlrtm1SgyApobtm1SgyTgN(Mx-Cre)1Cgn (Changed: 15-DEC-04
) | |
|
STOCK-Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy TgN(Mx1-Cre)1Cgn (Changed: 15-DEC-04
) | |
|
STOCK-Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy TgN(Mx1-cre)1Cgn (Changed: 15-DEC-04
) | |
| Genes & Alleles | Apob; Apobtm2Sgy; Ldlr; Ldlrtm1Her; Mttp; Mttptm2Sgy; Mx1; cre; Tg(Mx1-cre)1Cgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Stephen Young, Gladstone Institutes UCSF Generation F?+4p (30-MAY-04) Strain Description
These mice are homozygous for four different induced mutations. The cumulative result of these mutations is a mouse model in which hypercholesterolemia can be reversed. By themselves, the combined presence of the Ldlrtm1Sgy and Apobtm1Sgy targeted alleles results in mice with a high susceptibility to atherosclerosis and total plasma cholesterol levels of approximately 300 mg/dl. A functional microsomal triglyceride transfer protein gene (Mttp) is essential for establishing a hypercholesterolemic condition. By flanking the Mttp gene with loxP sites and including a Mx1-Cre transgene, it is possible to reduce total plasma cholesterol levels from 300 mg/dl to 30 mg/dl upon induction of the Cre recombinase by administering interferon alpha, interferon beta, or synthetic double-stranded RNA. This unique model is useful in research related to the mechanisms and events of atherosclerotic reversal. Mice homozygous for the targeted alleles are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Strain Development
The donating investigator combined existing induced alleles to create this model. See Stock No's 003902, 002077, 002877 and 003556.
Gene & Allele Details
| Allele Symbol | Apobtm2Sgy | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Stephen G Young | ||
| Common Name(s) | apo-B100; | ||
| Mutation Made By | Stephen Young, Gladstone Institutes UCSF | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Apob, apolipoprotein B | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | AI315052; Aa1064; Ac1-060; ApoB-100; ApoB-48; FLDB; expressed sequence AI315052; | ||
| Molecular Note | A "hit and run"-type vector was used to create a CTA to TTA mutation in codon 2153 in sequences corresponding to the apo-B48 editing codon. Western blot analysis on plasma derived from heterozygous and homozygous mice demostrated that the expression of the ApoB100 isoform is unaffected by this mutation, while no ApoB48 isoform is produced from this allele. [MGI Ref ID J:33830] | ||
| Allele Symbol | Ldlrtm1Her | ||
| Allele Name | targeted mutation 1, Joachim Herz | ||
| Common Name(s) | LDLR KO; LDLR-; LDLr0; LDLrKO; Ldlrtm1Her; | ||
| Mutation Made By | Joachim Herz, Univ of Texas Southwest Med Ctr Dallas | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Ldlr, low density lipoprotein receptor | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | FH; FHC; LDLRA; | ||
| General Note | When used in bone marrow transplant into Ldlrtm1Her homozygous mice, Abca1tm1Jdm Abcg1tm1Dgen homozygous cells accelerate the development of atherosclerosis. (J:130777) | ||
| Molecular Note | Insertion of a neomycin resistance cassette into exon 4. The authors predict that the targeted allele would encode a truncated non-functional protein that will not bind LDL, and that lacks a membrane spanning segment. Immunoblot analysis of liver membranes detected a truncated protein in homozygous mutant animals. [MGI Ref ID J:37394] | ||
| Allele Symbol | Mttptm2Sgy | ||
| Allele Name | targeted mutation 2, Steven G Young | ||
| Mutation Made By | Stephen Young, Gladstone Institutes UCSF | ||
| Strain of Origin | 129S4/SvJae | ||
| Site of Expression | cre mutation deletes floxed Mttp gene, reversing hypercholesterolemia phenotype | ||
| Gene Symbol and Name | Mttp, microsomal triglyceride transfer protein | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | ABL; MGC149819; MGC149820; MTP; | ||
| Molecular Note | A neomycin resistance cassette flanked by loxP sites was inserted into intron 1. An additional loxP site was inserted 2.5 kb upstream of the promoter and exon 1. [MGI Ref ID J:54612] | ||
| Allele Symbol | Tg(Mx1-cre)1Cgn | ||
| Allele Name | transgene insertion 1, University of Cologne | ||
| Common Name(s) | Mx-Cre; Mx-Cre 31; Mx1-Cre; Mx1cre; MxCre; | ||
| Mutation Made By | Ralf Kuhn, University of Cologne | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | widespread pattern of expression; promoter induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA; provides capability to induce the "knockout" at any time during development | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Mx1, myxovirus (influenza virus) resistance 1, mouse, laboratory | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of an inducible Mx1 promoter, which is silent in healthy mice, and active in the liver and in lymphocytes after induction with IFN or pI-pC. The construct also contains a 2.1 kb fragment from thehuman growth hormone gene. [MGI Ref ID J:105040] [MGI Ref ID J:67927] | ||
Genotyping Protocols
Apobtm2Sgy
Ldlr tm1Her
Colony Maintenance
| Breeding & Husbandry | Several strains contribute to the genetic background of this mutant mouse. Contributions from C57BL/6, CBA, 129S7/SvEvBrd-Hprtb-m2 and 129S4/SvJae are noted.Expected Coat color: Black and White Bellied Agouti. |
|---|
Related Strains
Strains carrying Apobtm2Sgy allele
002879 B6;129-Apobtm2Sgy Apoetm1Unc/J 003000 B6;129S-Apobtm2Sgy Ldlrtm1Her/J 002877 B6;129S7-Apobtm2Sgy/J View Strains carrying Apobtm2Sgy (3 strains)
002246 B6.129-Apoetm1Unc Ldlrtm1Her/J 002207 B6.129S7-Ldlrtm1Her/J 006952 B6.Cg-Akt2tm1.1Mbb Ldlrtm1Her/J 006580 B6.Cg-Ins2Akita Ldlrtm1Her/J 006883 B6.Cg-Ldlrtm1Her Sod2tm1Leb/J 006877 B6.Cg-Ldlrtm1Her Tg(H2-K-AKR1B1)1Tj/J 006906 B6.Cg-Lepob Ldlrtm1Her/J 002245 B6;129-Apoetm1Unc Ldlrtm1Her/J 003000 B6;129S-Apobtm2Sgy Ldlrtm1Her/J 002465 B6;129S-Ldlrtm1Her Lrpap1tm1Her/J 002077 B6;129S7-Ldlrtm1Her/J
003902 B6;129S-Mttptm2Sgy/J
006230 B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J 003556 B6.Cg-Tg(Mx1-cre)1Cgn/J 005673 C.Cg-Tg(Mx1-cre)1Cgn/J 002527 STOCK Tg(Mx1-cre)1Cgn/J
002053 B6.129P2-Apobtm1Unc/J 007682 B6.129X1-Apobtm1.1Zc/J 002878 B6;129-Apobtm1Sgy Apoetm1Unc/J 002876 B6;129S-Apobtm1Sgy/J 007683 CByJ.129X1(Cg)-Apobtm1.1Zc/J 007679 SWR.129X1(B6)-Apobtm1.1Zc/J
005061 C57BL/6J-LdlrHlb301/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Inducible)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Apobtm2Sgy relatedResearch Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Ldlrtm1Her relatedCardiovascular Research
Atherosclerosis
Hypocholesterolemia
Hypotriglyceridemia
Developmental Biology Research
Neural Tube Defects
Mouse/Human Gene Homologs
hypobetalipoproteinemia, familial
Neurobiology Research
Neural Tube Defects
Mttptm2Sgy relatedCardiovascular Research
Atherosclerosis
Hypercholesterolemia
Metabolism Research
Lipid Metabolism
Mouse/Human Gene Homologs
hypercholesterolemia, familial
Metabolism Research
Lipid Metabolism
References
Selected Reference(s)
Additional ReferencesVeniant MM; Withycombe S; Young SG. 2001. Lipoprotein size and atherosclerosis susceptibility in Apoe(-/-) and Ldlr(-/-) mice. Arterioscler Thromb Vasc Biol 21(10):1567-70. [PubMed: 11597927] [MGI Ref ID J:109864]
Price and Supply Information
| Strain Name: | STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J |
| Stock Number: | 004192 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $2470.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form