Strain Name: |
B6.Cg-Selplgtm1Fur/J |
|---|---|
Stock Number: |
004201 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Selpltm1Fur/J (Changed: 30-MAY-07
) |
| Genes & Alleles | Selplg; Selplgtm1Fur; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Bruce Furie, Harvard Med School/Beth Israel Deaconess Generation N5F?+12 (06-DEC-07) Strain Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No targeted allele product (mRNA or protein) is detected by Northern blot or immunoassay. Mutant mice exhibit mild neutrophilia. Impaired early neutrophil migration in thioglycolate-induced peritonitis is followed by a delayed recovery to nearly normal levels. Although early trauma-induced leukocyte adhesion and migration is greatly reduced and in vivo leukocyte rolling (leukocyte-endothelial cell interaction) in postcapillary venules is severely decreased, cytokine-induced/E selectin-mediated leukocyte rolling is only slightly reduced in the mutant mice. This mutant mouse strain represents a model that may be useful in studies of leukocyte adhesion and migration in the inflammatory response.Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a 5' portion of the Selpl coding sequence. The construct was electroporated into STOCK(129/Sv(75%),C57BL/6J(20%),SJL(5%)) derived WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were backcrossed to C57BL/6J mice. Heterozygotes were bred to generate homozygotes.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Selplgtm1Fur | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Bruce Furie | ||
| Common Name(s) | PSGL-1-; | ||
| Mutation Made By | Bruce Furie, Harvard Med School/Beth Israel Deaconess | ||
| Strain of Origin | STOCK 129/Sv and C57BL/6J and SJL | ||
| ES Cell Line Name | WW6 | ||
| ES Cell Line Strain | STOCK 129/Sv and C57BL/6J and SJL | ||
| Gene Symbol and Name | Selplg, selectin, platelet (p-selectin) ligand | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CD162; CLA; PSGL-1; PSGL1; Selpl; | ||
| Molecular Note | A neomycin resistance cassette replaced a small DNA segment of the coding region (+69 to 94). A 300 bp segment in the 5' flanking region containing CT repeats was also deleted. Northern blot analysis on thymus and spleen RNA from homozygous mice demonstrated that no detecable transcript was produced from this allele. Flow cytometry experiments on leukocytes derived from homozygous mice confirmed that no detectable cell surface protein was expressed from this allele. [MGI Ref ID J:75412] | ||
Control Information
| Allele | Control | |
|---|---|---|
| Selplgtm1Fur | 000664 C57BL/6J | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Selplgtm1Fur
Colony Maintenance
| Breeding & Husbandry | This strain originated on a STOCK background, has been backcrossed for 5 generations on the C57BL/6J background before being made homozygous. Coat color expected from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Selplg
006336 B6.129-Selplgtm1Rpmc/J View Strains carrying other alleles of Selplg (1 strain)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:Selplgtm1Fur related
Cell Biology Research
Defects in Cell Adhesion Molecules
Hematological Research
Clotting Defects
Hematopoietic Defects
Immunological Defects (B and T cell deficiency)
Platelet Defects (Alterations in platelet aggregation)
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (T cell deficiency)
Internal/Organ Research
Lymphoid Tissue Defects
Wound Healing
Research Tools
Hematological Research
Immunology and Inflammation Research
References
Selected Reference(s)
Additional ReferencesYang J; Hirata T; Croce K; Merrill-Skoloff G; Tchernychev B; Williams E; Flaumenhaft R; Furie BC; Furie B. 1999. Targeted gene disruption demonstrates that P-selectin glycoprotein ligand 1 (PSGL-1) is required for P-selectin-mediated but not E-selectin-mediated neutrophil rolling and migration. J Exp Med 190(12):1769-82. [PubMed: 10601352] [MGI Ref ID J:75412]
Price and Supply Information
| Strain Name: | B6.Cg-Selplgtm1Fur/J |
| Stock Number: | 004201 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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