Description

Strain Information

Former Names B6.Cg-Selpltm1Fur/J    (Changed: 30-MAY-07 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N5F?+14N1F0 (06-JAN-09)   Donating Investigator Bruce Furie,   Harvard Med School/Beth Israel Deaconess

Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No targeted allele product (mRNA or protein) is detected by Northern blot or immunoassay. Mutant mice exhibit mild neutrophilia. Impaired early neutrophil migration in thioglycolate-induced peritonitis is followed by a delayed recovery to nearly normal levels. Although early trauma-induced leukocyte adhesion and migration is greatly reduced and in vivo leukocyte rolling (leukocyte-endothelial cell interaction) in postcapillary venules is severely decreased, cytokine-induced/E selectin-mediated leukocyte rolling is only slightly reduced in the mutant mice. This mutant mouse strain represents a model that may be useful in studies of leukocyte adhesion and migration in the inflammatory response.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a 5' portion of the Selpl coding sequence. The construct was electroporated into STOCK(129/Sv(75%),C57BL/6J(20%),SJL(5%)) derived WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were backcrossed to C57BL/6J mice. Heterozygotes were bred to generate homozygotes.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Selplg

006336

B6.129-Selplgtm1Rpmc/J

View Strains carrying other alleles of Selplg     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Selplg^tm1Fur/Selplg^tm1Fur

        involves: 129/Sv * C57BL/6J * SJL

• immune system phenotype
• abnormal leukocyte rolling (MGI Ref ID J:75412)
  • impaired
• impaired neutrophil migration (MGI Ref ID J:75412)
  • during experimental peritonitis
• increased eosinophil cell number (MGI Ref ID J:75412)
  • 3.5-fold higher than in wild-type mice
• increased neutrophil cell number (MGI Ref ID J:75412)
  • 2.9-fold higher than in wild-type mice
• hematopoietic system phenotype
• increased eosinophil cell number (MGI Ref ID J:75412)
  • 3.5-fold higher than in wild-type mice
• increased neutrophil cell number (MGI Ref ID J:75412)
  • 2.9-fold higher than in wild-type mice

Genes & Alleles

Gene & Allele Information

Allele Symbol		Selplgtm1Fur
Allele Name		targeted mutation 1, Bruce Furie
Allele Type		Targeted (knock-out)
Common Name(s)		PSGL-1-;
Mutation Made By		Bruce Furie,   Harvard Med School/Beth Israel Deaconess
Strain of Origin		STOCK 129/Sv and C57BL/6J and SJL
ES Cell Line Name		WW6
ES Cell Line Strain		STOCK 129/Sv and C57BL/6J and SJL
Gene Symbol and Name		Selplg, selectin, platelet (p-selectin) ligand
Chromosome		5
Gene Common Name(s)		CD162; CLA; PSGL-1; PSGL1; Selpl;
Molecular Note		A neomycin resistance cassette replaced a small DNA segment of the coding region (+69 to 94). A 300 bp segment in the 5' flanking region containing CT repeats was also deleted. Northern blot analysis on thymus and spleen RNA from homozygous mice demonstrated that no detecable transcript was produced from this allele. Flow cytometry experiments on leukocytes derived from homozygous mice confirmed that no detectable cell surface protein was expressed from this allele. [MGI Ref ID J:75412]

Genotyping

Genotyping Information

Genotyping Protocols

Selplg^tm1Fur, STD PCR, vers. 1
Selplg^tm1Fur, STD PCR, vers. 2

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Yang J; Hirata T; Croce K; Merrill-Skoloff G; Tchernychev B; Williams E; Flaumenhaft R; Furie BC; Furie B. 1999. Targeted gene disruption demonstrates that P-selectin glycoprotein ligand 1 (PSGL-1) is required for P-selectin-mediated but not E-selectin-mediated neutrophil rolling and migration. J Exp Med 190(12):1769-82. [PubMed: 10601352]  [MGI Ref ID J:75412]

Additional References

Selplg^tm1Fur related

Alcaide P; King SL; Dimitroff CJ; Lim YC; Fuhlbrigge RC; Luscinskas FW. 2007. The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivo. J Invest Dermatol 127(8):1964-72. [PubMed: 17392823]  [MGI Ref ID J:141733]

Atarashi K; Hirata T; Matsumoto M; Kanemitsu N; Miyasaka M. 2005. Rolling of Th1 cells via P-selectin glycoprotein ligand-1 stimulates LFA-1-mediated cell binding to ICAM-1. J Immunol 174(3):1424-32. [PubMed: 15661900]  [MGI Ref ID J:96414]

Carlow DA; Ziltener HJ. 2006. CD43 deficiency has no impact in competitive in vivo assays of neutrophil or activated T cell recruitment efficiency. J Immunol 177(9):6450-9. [PubMed: 17056577]  [MGI Ref ID J:140506]

Chu YW; Schmitz S; Choudhury B; Telford W; Kapoor V; Garfield S; Howe D; Gress RE. 2008. Exogenous insulin-like growth factor 1 enhances thymopoiesis predominantly through thymic epithelial cell expansion. Blood 112(7):2836-46. [PubMed: 18658030]  [MGI Ref ID J:140225]

Dole VS; Bergmeier W; Mitchell HA; Eichenberger SC; Wagner DD. 2005. Activated platelets induce Weibel-Palade-body secretion and leukocyte rolling in vivo: role of P-selectin. Blood 106(7):2334-9. [PubMed: 15956287]  [MGI Ref ID J:119376]

Falati S; Liu Q; Gross P; Merrill-Skoloff G; Chou J; Vandendries E; Celi A; Croce K; Furie BC; Furie B. 2003. Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin. J Exp Med 197(11):1585-98. [PubMed: 12782720]  [MGI Ref ID J:83734]

Gossens K; Naus S; Corbel SY; Lin S; Rossi FM; Kast J; Ziltener HJ. 2009. Thymic progenitor homing and lymphocyte homeostasis are linked via S1P-controlled expression of thymic P-selectin/CCL25. J Exp Med 206(4):761-78. [PubMed: 19289576]  [MGI Ref ID J:147871]

Harakawa N; Shigeta A; Wato M; Merrill-Skoloff G; Furie BC; Furie B; Okazaki T; Domae N; Miyasaka M; Hirata T. 2007. P-selectin glycoprotein ligand-1 mediates L-selectin-independent leukocyte rolling in high endothelial venules of peripheral lymph nodes. Int Immunol 19(3):321-9. [PubMed: 17267415]  [MGI Ref ID J:118672]

He X; Schoeb TR; Panoskaltsis-Mortari A; Zinn KR; Kesterson RA; Zhang J; Samuel S; Hicks MJ; Hickey MJ; Bullard DC. 2006. Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice. J Immunol 177(12):8748-56. [PubMed: 17142777]  [MGI Ref ID J:127199]

Hidalgo A; Chang J; Jang JE; Peired AJ; Chiang EY; Frenette PS. 2009. Heterotypic interactions enabled by polarized neutrophil microdomains mediate thromboinflammatory injury. Nat Med 15(4):384-91. [PubMed: 19305412]  [MGI Ref ID J:149370]

Hidalgo A; Peired AJ; Wild MK; Vestweber D; Frenette PS. 2007. Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 26(4):477-89. [PubMed: 17442598]  [MGI Ref ID J:123577]

Hirata T; Furie BC; Furie B. 2002. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol 169(8):4307-13. [PubMed: 12370362]  [MGI Ref ID J:107382]

Hirata T; Merrill-Skoloff G; Aab M; Yang J; Furie BC; Furie B. 2000. P-Selectin glycoprotein ligand 1 (PSGL-1) is a physiological ligand for E-selectin in mediating T helper 1 lymphocyte migration. J Exp Med 192(11):1669-76. [PubMed: 11104809]  [MGI Ref ID J:115108]

Kerfoot SM; Norman MU; Lapointe BM; Bonder CS; Zbytnuik L; Kubes P. 2006. Reevaluation of P-selectin and alpha 4 integrin as targets for the treatment of experimental autoimmune encephalomyelitis. J Immunol 176(10):6225-34. [PubMed: 16670333]  [MGI Ref ID J:131757]

Mandiyan VS; Coats JK; Shah NM. 2005. Deficits in sexual and aggressive behaviors in Cnga2 mutant mice. Nat Neurosci 8(12):1660-2. [PubMed: 16261133]  [MGI Ref ID J:103844]

Matsumoto M; Shigeta A; Furukawa Y; Tanaka T; Miyasaka M; Hirata T. 2007. CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin. J Immunol 178(4):2499-506. [PubMed: 17277158]  [MGI Ref ID J:147254]

Matsumoto M; Shigeta A; Miyasaka M; Hirata T. 2008. CD43 plays both antiadhesive and proadhesive roles in neutrophil rolling in a context-dependent manner. J Immunol 181(5):3628-35. [PubMed: 18714037]  [MGI Ref ID J:138936]

Merzaban JS; Zuccolo J; Corbel SY; Williams MJ; Ziltener HJ. 2005. An alternate core 2 beta1,6-N-acetylglucosaminyltransferase selectively contributes to P-selectin ligand formation in activated CD8 T cells. J Immunol 174(7):4051-9. [PubMed: 15778363]  [MGI Ref ID J:97929]

Osmers I; Bullard DC; Barnum SR. 2005. PSGL-1 is not required for development of experimental autoimmune encephalomyelitis. J Neuroimmunol 166(1-2):193-6. [PubMed: 16005524]  [MGI Ref ID J:106363]

Ostanin DV; Furr KL; Pavlick KP; Gray L; Kevil CG; Shukla D; D'Souza D; Hoffman JM; Grisham MB. 2007. T cell-associated CD18 but not CD62L, ICAM-1, or PSGL-1 is required for the induction of chronic colitis. Am J Physiol Gastrointest Liver Physiol 292(6):G1706-14. [PubMed: 17332469]  [MGI Ref ID J:123696]

Rossi FM; Corbel SY; Merzaban JS; Carlow DA; Gossens K; Duenas J; So L; Yi L; Ziltener HJ. 2005. Recruitment of adult thymic progenitors is regulated by P-selectin and its ligand PSGL-1. Nat Immunol 6(6):626-34. [PubMed: 15880112]  [MGI Ref ID J:98951]

Shigeta A; Matsumoto M; Tedder TF; Lowe JB; Miyasaka M; Hirata T. 2008. An L-selectin ligand distinct from P-selectin glycoprotein ligand-1 is expressed on endothelial cells and promotes neutrophil rolling in inflammation. Blood 112(13):4915-23. [PubMed: 18818390]  [MGI Ref ID J:144251]

Veerman KM; Williams MJ; Uchimura K; Singer MS; Merzaban JS; Naus S; Carlow DA; Owen P; Rivera-Nieves J; Rosen SD; Ziltener HJ. 2007. Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs. Nat Immunol 8(5):532-9. [PubMed: 17401367]  [MGI Ref ID J:122420]

Wang HB; Wang JT; Zhang L; Geng ZH; Xu WL; Xu T; Huo Y; Zhu X; Plow EF; Chen M; Geng JG. 2007. P-selectin primes leukocyte integrin activation during inflammation. Nat Immunol 8(8):882-892. [PubMed: 17632516]  [MGI Ref ID J:123408]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	This strain originated on a STOCK background, has been backcrossed for 5 generations on the C57BL/6J background before being made homozygous. Coat color expected from breeding:Black
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
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