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Strain Name:

B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J

Stock Number:

004218

Availability:

Repository- Live


General Terms and Conditions

Former Name      B6.129(ICR)-Tg(ACTB-ECFP)CK6Nagy/J    (Changed: 08-MAY-08 )
      B6.129(ICR)-Tg(ACTB-ECFP)1Nagy/J    (Changed: 08-NOV-06 )
      B6.129(ICR)-Tg(ActbECFP)1Nagy/J    (Changed: 29-MAR-05 )
Genes & Alleles   ACTB;   CFP;   Tg(CAG-ECFP)CK6Nagy;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator IMR Colony,   The Jackson Laboratory
GenerationN6F14 (06-DEC-07)

Strain Description
Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Strain Development
A transgenic construct containing an Enhanced Cyan Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line CK6/ECFP were sent to The Jackson Laboratory in 2000 and used to establish the colony on a mixed genetic background (see Stock No. 003773). From there, some mice were then backcrossed for more than 5 generations to C57BL/6J mice to establish this congenic strain.

Gene & Allele Details

Allele Symbol Tg(CAG-ECFP)CK6Nagy
Allele Name transgene insertion CK6, Andras Nagy
Common Name(s) CK6/ECFP; Tg(ACTB-ECFP)CK6Nagy; Tg(ActbECFP)1Nagy; beta-actin-CFP;
Mutation Made By Anna-Katerina Hadjantonakis,   Memorial Sloan-Kettering Cancer Center
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Site of ExpressionAll tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes, in which fluorescence is negligible or absent.
Expressed Gene CFP, Cyan Fluorescent Protein, jellyfish
Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When CFP is utilized in a transgenic construct, tissue expressing sufficient amounts of CFP will fluoresce cyan when exposed to a 433 nm light source.
Promoter ACTB, actin, beta, chicken
General Note Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. All tissues from these animals display fluorescence in all cell types under appropriate lighting conditions, except in erythrocytes and adipocytes where fluorescence is negligible or absent.
Molecular Note The transgene construct contains an Enhanced Cyan Fluorescent Protein gene under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP). R1 ES cells were used to introduce the transgene. [J:93696]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Fluorescent Proteins

Colony Maintenance

Breeding & HusbandryThis strain originated on a stock background consisting of contributions from 129-derived R1 ES cells and ICR mice. Mice were backcrossed to C57BL/6J. Coat color expected from breeding:Black
Diet Information LabDiet® 5K52/5K67

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003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
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003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
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006334   STOCK Tg(Gad1-EGFP)94Agmo/J
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006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
006784   STOCK Tg(Ins1-ECFP)24Hara/J
003658   STOCK Tg(TIE2GFP)287Sato/J
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003274   STOCK Tg(tetNZL)2Bjd/J
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005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Fluorescent Protein Strains     (135 strains)

Strains carrying   Tg(CAG-ECFP)CK6Nagy allele
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
View Strains carrying   Tg(CAG-ECFP)CK6Nagy     (1 strain)

View Strains carrying other alleles of ACTB     (27 strains)

View Strains carrying other alleles of CFP     (11 strains)

Additional Web Information

Congenic Nomenclature
Fluorescent Proteins/lacZ Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)

CFP related

Research Tools
Fluorescent Proteins

References

Selected Reference(s)

Hadjantonakis AK; Macmaster S; Nagy A. 2002. Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol 2(1):11. [PubMed: 12079497]  [J:93696]

Additional References

Price and Supply Information

Strain Name: B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J
Stock Number: 004218

Price Details

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Supply Details

Standard SupplyRepository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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