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- Description
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Description
Strain Information
Former Names B6.129(ICR)-Tg(ACTB-ECFP)CK6Nagy/J (Changed: 08-MAY-08 ) B6.129(ICR)-Tg(ACTB-ECFP)1Nagy/J (Changed: 08-NOV-06 ) B6.129(ICR)-Tg(ActbECFP)1Nagy/J (Changed: 29-MAR-05 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 18-DEC-09 Species laboratory mouse Generation N6F14N1F1 (06-JAN-09)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing an Enhanced Cyan Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line CK6/ECFP were sent to The Jackson Laboratory in 2000 and used to establish the colony on a mixed genetic background (see Stock No. 003773). From there, some mice were then backcrossed for more than 5 generations to C57BL/6J mice to establish this congenic strain.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
003773 STOCK Tg(CAG-ECFP)CK6Nagy/J
008513 B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 014131 B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 013161 B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 007879 STOCK Stx1atm2Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-Cerulean)24Hara/J 016922 STOCK Tg(Myh7-CFP)1Jrs/J 013162 STOCK Tg(Thy1-Clomeleon)12Gjau/J 013163 STOCK Tg(Thy1-Clomeleon)13Gjau/J
003773 STOCK Tg(CAG-ECFP)CK6Nagy/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-ECFP)CK6Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion CK6, Andras Nagy | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | CK6/ECFP; Tg(ACTB-ECFP); Tg(ACTB-ECFP)1Nagy; Tg(ACTB-ECFP)CK6Nagy; Tg(ActbECFP)1Nagy; beta-actin-CFP; | ||
| Mutation Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes, in which fluorescence is negligible or absent. | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. All tissues from these animals display fluorescence in all cell types under appropriate lighting conditions, except in erythrocytes and adipocytes where fluorescence is negligible or absent. | ||
| Molecular Note | The transgene construct contains an enhanced cyan fluorescent protein gene driven by the cytomegalovirus (CMV) immediate early enhancer coupled to the chicken beta actin promoter. [MGI Ref ID J:93696] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hadjantonakis AK; Macmaster S; Nagy A. 2002. Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol 2(1):11. [PubMed: 12079497] [MGI Ref ID J:93696]
Additional References
Tg(CAG-ECFP)CK6Nagy relatedAllen CD; Okada T; Tang HL; Cyster JG. 2007. Imaging of germinal center selection events during affinity maturation. Science 315(5811):528-31. [PubMed: 17185562] [MGI Ref ID J:118931]
Dykstra B; Olthof S; Schreuder J; Ritsema M; de Haan G. 2011. Clonal analysis reveals multiple functional defects of aged murine hematopoietic stem cells. J Exp Med 208(13):2691-703. [PubMed: 22110168] [MGI Ref ID J:179054]
Grigorova IL; Schwab SR; Phan TG; Pham TH; Okada T; Cyster JG. 2009. Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells. Nat Immunol 10(1):58-65. [PubMed: 19060900] [MGI Ref ID J:142339]
Hugues S; Scholer A; Boissonnas A; Nussbaum A; Combadiere C; Amigorena S; Fetler L. 2007. Dynamic imaging of chemokine-dependent CD8(+) T cell help for CD8(+) T cell responses. Nat Immunol 8(9):921-30. [PubMed: 17660821] [MGI Ref ID J:124274]
Jacobelli J; Friedman RS; Conti MA; Lennon-Dumenil AM; Piel M; Sorensen CM; Adelstein RS; Krummel MF. 2010. Confinement-optimized three-dimensional T cell amoeboid motility is modulated via myosin IIA-regulated adhesions. Nat Immunol 11(10):953-61. [PubMed: 20835229] [MGI Ref ID J:164684]
Kitano M; Moriyama S; Ando Y; Hikida M; Mori Y; Kurosaki T; Okada T. 2011. Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity. Immunity 34(6):961-72. [PubMed: 21636294] [MGI Ref ID J:174014]
Lindquist RL; Shakhar G; Dudziak D; Wardemann H; Eisenreich T; Dustin ML; Nussenzweig MC. 2004. Visualizing dendritic cell networks in vivo. Nat Immunol 5(12):1243-50. [PubMed: 15543150] [MGI Ref ID J:141750]
Mayack SR; Wagers AJ. 2008. Osteolineage niche cells initiate hematopoietic stem cell mobilization. Blood 112(3):519-31. [PubMed: 18456874] [MGI Ref ID J:139218]
Mionnet C; Sanos SL; Mondor I; Jorquera A; Laugier JP; Germain RN; Bajenoff M. 2011. High endothelial venules as traffic control points maintaining lymphocyte population homeostasis in lymph nodes. Blood 118(23):6115-22. [PubMed: 21937697] [MGI Ref ID J:179107]
Moraes RC; Chang H; Harrington N; Landua JD; Prigge JT; Lane TF; Wainwright BJ; Hamel PA; Lewis MT. 2009. Ptch1 is required locally for mammary gland morphogenesis and systemically for ductal elongation. Development 136(9):1423-32. [PubMed: 19297414] [MGI Ref ID J:147994]
Phan TG; Green JA; Gray EE; Xu Y; Cyster JG. 2009. Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation. Nat Immunol 10(7):786-93. [PubMed: 19503106] [MGI Ref ID J:150134]
Phan TG; Grigorova I; Okada T; Cyster JG. 2007. Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells. Nat Immunol 8(9):992-1000. [PubMed: 17660822] [MGI Ref ID J:124273]
Schaeffer M; Han SJ; Chtanova T; van Dooren GG; Herzmark P; Chen Y; Roysam B; Striepen B; Robey EA. 2009. Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii. J Immunol 182(10):6379-93. [PubMed: 19414791] [MGI Ref ID J:148321]
Sullivan BM; Liang HE; Bando JK; Wu D; Cheng LE; McKerrow JK; Allen CD; Locksley RM. 2011. Genetic analysis of basophil function in vivo. Nat Immunol 12(6):527-35. [PubMed: 21552267] [MGI Ref ID J:172122]
Tadokoro CE; Shakhar G; Shen S; Ding Y; Lino AC; Maraver A; Lafaille JJ; Dustin ML. 2006. Regulatory T cells inhibit stable contacts between CD4+ T cells and dendritic cells in vivo. J Exp Med 203(3):505-11. [PubMed: 16533880] [MGI Ref ID J:123760]
Woolf E; Grigorova I; Sagiv A; Grabovsky V; Feigelson SW; Shulman Z; Hartmann T; Sixt M; Cyster JG; Alon R. 2007. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces. Nat Immunol 8(10):1076-85. [PubMed: 17721537] [MGI Ref ID J:125276]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 18-DEC-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $155.00 Female or Male Homozygous for Tg(CAG-ECFP)CK6Nagy
Pairs /Price (US dollars $) Pair Genotype $310.00 Homozygous for Tg(CAG-ECFP)CK6Nagy x Homozygous for Tg(CAG-ECFP)CK6Nagy Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $201.50 Female or Male Homozygous for Tg(CAG-ECFP)CK6Nagy
Pairs /Price (US dollars $) Pair Genotype $403.00 Homozygous for Tg(CAG-ECFP)CK6Nagy x Homozygous for Tg(CAG-ECFP)CK6Nagy Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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