| |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.129(ICR)-Tg(ACTB-ECFP)CK6Nagy/J (Changed: 08-MAY-08 ) B6.129(ICR)-Tg(ACTB-ECFP)1Nagy/J (Changed: 08-NOV-06 ) B6.129(ICR)-Tg(ActbECFP)1Nagy/J (Changed: 29-MAR-05 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Tg/? x Tg/? (Female x Male) 23-JAN-09 Species laboratory mouse Generation N6F14N1F1 (06-JAN-09) Donating Investigator IMR Colony, The Jackson Laboratory Description
Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing an Enhanced Cyan Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line CK6/ECFP were sent to The Jackson Laboratory in 2000 and used to establish the colony on a mixed genetic background (see Stock No. 003773). From there, some mice were then backcrossed for more than 5 generations to C57BL/6J mice to establish this congenic strain.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
003773 STOCK Tg(CAG-ECFP)CK6Nagy/J
007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 006617 B6;CBA-Tg(Thy1-CFP/COX8A)S2Lich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 006784 STOCK Tg(Ins1-ECFP)24Hara/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
CFP relatedResearch Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-ECFP)CK6Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion CK6, Andras Nagy | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | CK6/ECFP; Tg(ACTB-ECFP); Tg(ACTB-ECFP)1Nagy; Tg(ACTB-ECFP)CK6Nagy; Tg(ActbECFP)1Nagy; Tg(CAG-ECFP)CK6Nagy; beta-actin-CFP; | ||
| Mutation Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes, in which fluorescence is negligible or absent. | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When CFP is utilized in a transgenic construct, tissue expressing sufficient amounts of CFP will fluoresce cyan when exposed to a 433 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. All tissues from these animals display fluorescence in all cell types under appropriate lighting conditions, except in erythrocytes and adipocytes where fluorescence is negligible or absent. | ||
| Molecular Note | The transgene construct contains an enhanced cyan fluorescent protein gene driven by the cytomegalovirus (CMV) immediate early enhancer coupled to the chicken beta actin promoter. [MGI Ref ID J:93696] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hadjantonakis AK; Macmaster S; Nagy A. 2002. Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol 2(1):11. [PubMed: 12079497] [MGI Ref ID J:93696]
Additional References
Tg(CAG-ECFP)CK6Nagy relatedAllen CD; Okada T; Tang HL; Cyster JG. 2007. Imaging of germinal center selection events during affinity maturation. Science 315(5811):528-31. [PubMed: 17185562] [MGI Ref ID J:118931]
Grigorova IL; Schwab SR; Phan TG; Pham TH; Okada T; Cyster JG. 2009. Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells. Nat Immunol 10(1):58-65. [PubMed: 19060900] [MGI Ref ID J:142339]
Hugues S; Scholer A; Boissonnas A; Nussbaum A; Combadiere C; Amigorena S; Fetler L. 2007. Dynamic imaging of chemokine-dependent CD8(+) T cell help for CD8(+) T cell responses. Nat Immunol 8(9):921-30. [PubMed: 17660821] [MGI Ref ID J:124274]
Lindquist RL; Shakhar G; Dudziak D; Wardemann H; Eisenreich T; Dustin ML; Nussenzweig MC. 2004. Visualizing dendritic cell networks in vivo. Nat Immunol 5(12):1243-50. [PubMed: 15543150] [MGI Ref ID J:141750]
Mayack SR; Wagers AJ. 2008. Osteolineage niche cells initiate hematopoietic stem cell mobilization. Blood 112(3):519-31. [PubMed: 18456874] [MGI Ref ID J:139218]
Moraes RC; Chang H; Harrington N; Landua JD; Prigge JT; Lane TF; Wainwright BJ; Hamel PA; Lewis MT. 2009. Ptch1 is required locally for mammary gland morphogenesis and systemically for ductal elongation. Development 136(9):1423-32. [PubMed: 19297414] [MGI Ref ID J:147994]
Phan TG; Green JA; Gray EE; Xu Y; Cyster JG. 2009. Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation. Nat Immunol 10(7):786-93. [PubMed: 19503106] [MGI Ref ID J:150134]
Phan TG; Grigorova I; Okada T; Cyster JG. 2007. Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells. Nat Immunol 8(9):992-1000. [PubMed: 17660822] [MGI Ref ID J:124273]
Schaeffer M; Han SJ; Chtanova T; van Dooren GG; Herzmark P; Chen Y; Roysam B; Striepen B; Robey EA. 2009. Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii. J Immunol 182(10):6379-93. [PubMed: 19414791] [MGI Ref ID J:148321]
Tadokoro CE; Shakhar G; Shen S; Ding Y; Lino AC; Maraver A; Lafaille JJ; Dustin ML. 2006. Regulatory T cells inhibit stable contacts between CD4+ T cells and dendritic cells in vivo. J Exp Med 203(3):505-11. [PubMed: 16533880] [MGI Ref ID J:123760]
Woolf E; Grigorova I; Sagiv A; Grabovsky V; Feigelson SW; Shulman Z; Hartmann T; Sixt M; Cyster JG; Alon R. 2007. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces. Nat Immunol 8(10):1076-85. [PubMed: 17721537] [MGI Ref ID J:125276]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together, to noncarrier (wildtype) siblings, or to C57BL/6J inbred mice. Mating System Tg/? x Tg/? (Female x Male) 23-JAN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $111.20 Female or Male Hemizygous or Homozygous for Tg(CAG-ECFP)CK6Nagy
Pairs /Price (US dollars $) Pair Genotype $222.40 Hemizygous or Homozygous for Tg(CAG-ECFP)CK6Nagy x Hemizygous or Homozygous for Tg(CAG-ECFP)CK6Nagy
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $144.60 Female or Male Hemizygous or Homozygous for Tg(CAG-ECFP)CK6Nagy
Pairs /Price (US dollars $) Pair Genotype $289.20 Hemizygous or Homozygous for Tg(CAG-ECFP)CK6Nagy x Hemizygous or Homozygous for Tg(CAG-ECFP)CK6Nagy
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.