Strain Name: |
B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J |
|---|---|
Stock Number: |
004218 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6.129(ICR)-Tg(ACTB-ECFP)CK6Nagy/J (Changed: 08-MAY-08
) |
|
B6.129(ICR)-Tg(ACTB-ECFP)1Nagy/J (Changed: 08-NOV-06
) | |
|
B6.129(ICR)-Tg(ActbECFP)1Nagy/J (Changed: 29-MAR-05
) | |
| Genes & Alleles | ACTB; CFP; Tg(CAG-ECFP)CK6Nagy; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N6F14 (06-DEC-07) Strain Description
Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing an Enhanced Cyan Fluorescent Protein gene (Clontech) under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells containing the construct were aggregated with ICR morulae and the resulting chimeric males were bred with ICR females for germline transmission. The transgenic offspring were intercrossed to generate homozygotes. Mice derived from founder line CK6/ECFP were sent to The Jackson Laboratory in 2000 and used to establish the colony on a mixed genetic background (see Stock No. 003773). From there, some mice were then backcrossed for more than 5 generations to C57BL/6J mice to establish this congenic strain.
Gene & Allele Details
| Allele Symbol | Tg(CAG-ECFP)CK6Nagy | ||
|---|---|---|---|
| Allele Name | transgene insertion CK6, Andras Nagy | ||
| Common Name(s) | CK6/ECFP; Tg(ACTB-ECFP)1Nagy; Tg(ACTB-ECFP)CK6Nagy; Tg(ActbECFP)1Nagy; Tg(CAG-ECFP)CK6Nagy; beta-actin-CFP; | ||
| Mutation Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Notable exceptions to this phenotype are erythrocytes and adipocytes, in which fluorescence is negligible or absent. | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When CFP is utilized in a transgenic construct, tissue expressing sufficient amounts of CFP will fluoresce cyan when exposed to a 433 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. All tissues from these animals display fluorescence in all cell types under appropriate lighting conditions, except in erythrocytes and adipocytes where fluorescence is negligible or absent. | ||
| Molecular Note | The transgene construct contains an Enhanced Cyan Fluorescent Protein gene under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP). R1 ES cells were used to introduce the transgene. [MGI Ref ID J:93696] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | This strain originated on a stock background consisting of contributions from 129-derived R1 ES cells and ICR mice. Mice were backcrossed to C57BL/6J. Coat color expected from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (146 strains)
003773 STOCK Tg(CAG-ECFP)CK6Nagy/J
007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 006614 B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J 006617 B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 006784 STOCK Tg(Ins1-ECFP)24Hara/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
CFP relatedResearch Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesHadjantonakis AK; Macmaster S; Nagy A. 2002. Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol 2(1):11. [PubMed: 12079497] [MGI Ref ID J:93696]
Price and Supply Information
| Strain Name: | B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J |
| Stock Number: | 004218 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $140.60 | Homozygous for Tg(CAG-ECFP)CK6Nagy | |||
| Pair | $281.10 | Homozygous for Tg(CAG-ECFP)CK6Nagy x Homozygous for Tg(CAG-ECFP)CK6Nagy | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form