Strain Name:

NOD.B6-Tg(KLK4mHEL)6Ccg/Dvs

Stock Number:

004258

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names NOD.B6-TgN(KLK4mHEL)6Ccg/Dvs    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse

Appearance
albino
Related Genotype: A/? Tyrc/Tyrc

Development
Tg(KLK4mHEL) was backcrossed from C57BL/6-Tg(KLK4mHEL)6Ccg (stock#002598) onto NOD/Lt in the laboratory of Dr. David Serreze. In 2004 this strain reached N13.

Related Strains

Strains carrying   Tg(KLK4mHEL)6Ccg allele
002598   C57BL/6-Tg(KLK4mHEL)6Ccg/J
View Strains carrying   Tg(KLK4mHEL)6Ccg     (1 strain)

View Strains carrying other alleles of H2-K     (17 strains)

View Strains carrying other alleles of HEL     (4 strains)

Phenotype

Phenotype Information

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(KLK4mHEL)6Ccg
Allele Name transgene insertion 6, Christopher C Goodnow
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) KLK-4; KLK3; mHEL; mHEL-KK;
Mutation Made ByDr. Christopher Goodnow,   Stanford University School of Medicine
Strain of OriginC57BL/6JSfd
Expressed Gene HEL, HEL, chicken
Promoter H2-K, histocompatibility 2, K region, mouse, laboratory
Molecular Note A genomic fragment containing the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) was linked to the ubiquitously-active mouse H2-Kb promoter and transmembrane sequence (which included the extracellular spacer sequence, transmembrane segment, and cytoplasmic tail). Fluorescence activated cell sorting (FACS) analysis of cells from spleen, lymph node, bone marrow, and thymus from mice carrying the transgene showed membrane expression of chicken lysozyme on these cells. [MGI Ref ID J:78307]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(KLK4mHEL)6Ccg, Tg(ML5sHEL)5Ccg, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Tg(KLK4mHEL)6Ccg related

Akkaraju S; Ho WY; Leong D; Canaan K; Davis MM; Goodnow CC. 1997. A range of CD4 T cell tolerance: partial inactivation to organ-specific antigen allows nondestructive thyroiditis or insulitis. Immunity 7(2):255-71. [PubMed: 9285410]  [MGI Ref ID J:78309]

Cornall RJ; Cheng AM; Pawson T; Goodnow CC. 2000. Role of Syk in B-cell development and antigen-receptor signaling. Proc Natl Acad Sci U S A 97(4):1713-8. [PubMed: 10677523]  [MGI Ref ID J:60647]

Cyster JG; Goodnow CC. 1995. Protein tyrosine phosphatase 1C negatively regulates antigen receptor signaling in B lymphocytes and determines thresholds for negative selection. Immunity 2(1):13-24. [PubMed: 7600299]  [MGI Ref ID J:28348]

Desmarets M; Cadwell CM; Peterson KR; Neades R; Zimring JC. 2009. Minor histocompatibility antigens on transfused leukoreduced units of red blood cells induce bone marrow transplant rejection in a mouse model. Blood 114(11):2315-22. [PubMed: 19525479]  [MGI Ref ID J:152426]

Hartley SB; Crosbie J; Brink R; Kantor AB; Basten A; Goodnow CC. 1991. Elimination from peripheral lymphoid tissues of self-reactive B lymphocytes recognizing membrane-bound antigens. Nature 353(6346):765-9. [PubMed: 1944535]  [MGI Ref ID J:78307]

Hartley SB; Goodnow CC. 1994. Censoring of self-reactive B cells with a range of receptor affinities in transgenic mice expressing heavy chains for a lysozyme-specific antibody. Int Immunol 6(9):1417-25. [PubMed: 7819151]  [MGI Ref ID J:132751]

Hippen KL; Schram BR; Tze LE; Pape KA; Jenkins MK; Behrens TW. 2005. In vivo assessment of the relative contributions of deletion, anergy, and editing to B cell self-tolerance. J Immunol 175(2):909-16. [PubMed: 16002689]  [MGI Ref ID J:100671]

Kumar KR; Li L; Yan M; Bhaskarabhatla M; Mobley AB; Nguyen C; Mooney JM; Schatzle JD; Wakeland EK; Mohan C. 2006. Regulation of B cell tolerance by the lupus susceptibility gene Ly108. Science 312(5780):1665-9. [PubMed: 16778059]  [MGI Ref ID J:109640]

Liston A; Gray DH; Lesage S; Fletcher AL; Wilson J; Webster KE; Scott HS; Boyd RL; Peltonen L; Goodnow CC. 2004. Gene dosage--limiting role of Aire in thymic expression, clonal deletion, and organ-specific autoimmunity. J Exp Med 200(8):1015-26. [PubMed: 15492124]  [MGI Ref ID J:93912]

Liu H; Zheng F; Cao Q; Ren B; Zhu L; Striker G; Vlassara H. 2006. Amelioration of oxidant stress by the defensin lysozyme. Am J Physiol Endocrinol Metab 290(5):E824-32. [PubMed: 16317028]  [MGI Ref ID J:108413]

Mecklenbrauker I; Saijo K; Zheng NY; Leitges M; Tarakhovsky A. 2002. Protein kinase Cdelta controls self-antigen-induced B-cell tolerance. Nature 416(6883):860-5. [PubMed: 11976686]  [MGI Ref ID J:76134]

Neighbors M; Hartley SB; Xu X; Castro AG; Bouley DM; O'Garra A. 2006. Breakpoints in immunoregulation required for Th1 cells to induce diabetes. Eur J Immunol 36(9):2315-23. [PubMed: 16933361]  [MGI Ref ID J:116743]

Pathak S; Ma S; Trinh L; Lu R. 2008. A role for interferon regulatory factor 4 in receptor editing. Mol Cell Biol 28(8):2815-24. [PubMed: 18285461]  [MGI Ref ID J:133924]

Phan TG; Amesbury M; Gardam S; Crosbie J; Hasbold J; Hodgkin PD; Basten A; Brink R. 2003. B cell receptor-independent stimuli trigger immunoglobulin (Ig) class switch recombination and production of IgG autoantibodies by anergic self-reactive B cells. J Exp Med 197(7):845-60. [PubMed: 12668643]  [MGI Ref ID J:132538]

Schram BR; Tze LE; Ramsey LB; Liu J; Najera L; Vegoe AL; Hardy RR; Hippen KL; Farrar MA; Behrens TW. 2008. B cell receptor Basal signaling regulates antigen-induced Ig light chain rearrangements. J Immunol 180(7):4728-41. [PubMed: 18354197]  [MGI Ref ID J:133383]

Silveira PA; Dombrowsky J; Johnson E; Chapman HD; Nemazee D; Serreze DV. 2004. B cell selection defects underlie the development of diabetogenic APCs in nonobese diabetic mice. J Immunol 172(8):5086-94. [PubMed: 15067092]  [MGI Ref ID J:89156]

Silver K; Bouriez-Jones T; Crockford T; Ferry H; Tang HL; Cyster JG; Cornall RJ. 2006. Spontaneous class switching and B cell hyperactivity increase autoimmunity against intracellular self antigen in Lyn-deficient mice. Eur J Immunol 36(11):2920-7. [PubMed: 17039569]  [MGI Ref ID J:116999]

Silver K; Ferry H; Crockford T; Cornall RJ. 2006. TLR4, TLR9 and MyD88 are not required for the positive selection of autoreactive B cells into the primary repertoire. Eur J Immunol 36(6):1404-12. [PubMed: 16703567]  [MGI Ref ID J:115059]

Smith NH; Hod EA; Spitalnik SL; Zimring JC; Hendrickson JE. 2012. Transfusion in the absence of inflammation induces antigen-specific tolerance to murine RBCs. Blood 119(6):1566-9. [PubMed: 22077064]  [MGI Ref ID J:181810]

Zwickey HL; Unternaehrer JJ; Mellman I. 2006. Presentation of self-antigens on MHC class II molecules during dendritic cell maturation. Int Immunol 18(1):199-209. [PubMed: 16361313]  [MGI Ref ID J:104201]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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