Strain Name:

NOD.B6-Tg(KLK4mHEL)6Ccg/Dvs

Stock Number:

004258

Availability:

Repository-Cryopreserved

Description

Strain Information

Former Names NOD.B6-TgN(KLK4mHEL)6Ccg/Dvs    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse

Appearance
albino
Related Genotype: A/? Tyrc/Tyrc

Development
Tg(KLK4mHEL) was backcrossed from C57BL/6-Tg(KLK4mHEL)6Ccg (stock#002598) onto NOD/Lt in the laboratory of Dr. David Serreze. In 2004 this strain reached N13.

Related Strains

Strains carrying   Tg(KLK4mHEL)6Ccg allele
002598   C57BL/6-Tg(KLK4mHEL)6Ccg/J
View Strains carrying   Tg(KLK4mHEL)6Ccg     (1 strain)

View Strains carrying other alleles of H2-K     (8 strains)

Strains carrying other alleles of HEL
002599   C57BL/6-Tg(ML5sHEL)5Ccg/J
004252   NOD.B6-Tg(ML5sHEL)5Ccg/Dvs
006610   NOD.B6-Tg(ML5sHEL)5Ccg/DvsJ
View Strains carrying other alleles of HEL     (3 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

HEL related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(KLK4mHEL)6Ccg
Allele Name transgene insertion 6, Christopher C Goodnow
Allele Type Transgenic (random, expressed)
Common Name(s) KLK-4; KLK3; mHEL; mHEL-KK;
Mutation Made By Christopher Goodnow,   Stanford University School of Medicine
Strain of OriginC57BL/6
Expressed Gene HEL, HEL, chicken
Promoter H2-K, histocompatibility 2, K region, mouse, laboratory
Molecular Note A genomic fragment containing the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) was linked to the ubiquitously-active mouse H2-Kb promoter and transmembrane sequence (which included the extracellular spacer sequence, transmembrane segment, and cytoplasmic tail). Fluorescence activated cell sorting (FACS) analysis of cells from spleen, lymph node, bone marrow, and thymus from mice carrying the transgene showed membrane expression of chicken lysozyme on these cells. [MGI Ref ID J:78307]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(KLK4mHEL)6Ccg, Tg(ML5sHEL)5Ccg, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Tg(KLK4mHEL)6Ccg related

Akkaraju S; Ho WY; Leong D; Canaan K; Davis MM; Goodnow CC. 1997. A range of CD4 T cell tolerance: partial inactivation to organ-specific antigen allows nondestructive thyroiditis or insulitis. Immunity 7(2):255-71. [PubMed: 9285410]  [MGI Ref ID J:78309]

Cornall RJ; Cheng AM; Pawson T; Goodnow CC. 2000. Role of Syk in B-cell development and antigen-receptor signaling. Proc Natl Acad Sci U S A 97(4):1713-8. [PubMed: 10677523]  [MGI Ref ID J:60647]

Cyster JG; Goodnow CC. 1995. Protein tyrosine phosphatase 1C negatively regulates antigen receptor signaling in B lymphocytes and determines thresholds for negative selection. Immunity 2(1):13-24. [PubMed: 7600299]  [MGI Ref ID J:28348]

Hartley SB; Crosbie J; Brink R; Kantor AB; Basten A; Goodnow CC. 1991. Elimination from peripheral lymphoid tissues of self-reactive B lymphocytes recognizing membrane-bound antigens. Nature 353(6346):765-9. [PubMed: 1944535]  [MGI Ref ID J:78307]

Hartley SB; Goodnow CC. 1994. Censoring of self-reactive B cells with a range of receptor affinities in transgenic mice expressing heavy chains for a lysozyme-specific antibody. Int Immunol 6(9):1417-25. [PubMed: 7819151]  [MGI Ref ID J:132751]

Hippen KL; Schram BR; Tze LE; Pape KA; Jenkins MK; Behrens TW. 2005. In vivo assessment of the relative contributions of deletion, anergy, and editing to B cell self-tolerance. J Immunol 175(2):909-16. [PubMed: 16002689]  [MGI Ref ID J:100671]

Kumar KR; Li L; Yan M; Bhaskarabhatla M; Mobley AB; Nguyen C; Mooney JM; Schatzle JD; Wakeland EK; Mohan C. 2006. Regulation of B cell tolerance by the lupus susceptibility gene Ly108. Science 312(5780):1665-9. [PubMed: 16778059]  [MGI Ref ID J:109640]

Liston A; Gray DH; Lesage S; Fletcher AL; Wilson J; Webster KE; Scott HS; Boyd RL; Peltonen L; Goodnow CC. 2004. Gene dosage--limiting role of Aire in thymic expression, clonal deletion, and organ-specific autoimmunity. J Exp Med 200(8):1015-26. [PubMed: 15492124]  [MGI Ref ID J:93912]

Liu H; Zheng F; Cao Q; Ren B; Zhu L; Striker G; Vlassara H. 2006. Amelioration of oxidant stress by the defensin lysozyme. Am J Physiol Endocrinol Metab 290(5):E824-32. [PubMed: 16317028]  [MGI Ref ID J:108413]

Neighbors M; Hartley SB; Xu X; Castro AG; Bouley DM; O'Garra A. 2006. Breakpoints in immunoregulation required for Th1 cells to induce diabetes. Eur J Immunol 36(9):2315-23. [PubMed: 16933361]  [MGI Ref ID J:116743]

Pathak S; Ma S; Trinh L; Lu R. 2008. A role for interferon regulatory factor 4 in receptor editing. Mol Cell Biol 28(8):2815-24. [PubMed: 18285461]  [MGI Ref ID J:133924]

Phan TG; Amesbury M; Gardam S; Crosbie J; Hasbold J; Hodgkin PD; Basten A; Brink R. 2003. B cell receptor-independent stimuli trigger immunoglobulin (Ig) class switch recombination and production of IgG autoantibodies by anergic self-reactive B cells. J Exp Med 197(7):845-60. [PubMed: 12668643]  [MGI Ref ID J:132538]

Schram BR; Tze LE; Ramsey LB; Liu J; Najera L; Vegoe AL; Hardy RR; Hippen KL; Farrar MA; Behrens TW. 2008. B cell receptor Basal signaling regulates antigen-induced Ig light chain rearrangements. J Immunol 180(7):4728-41. [PubMed: 18354197]  [MGI Ref ID J:133383]

Silveira PA; Dombrowsky J; Johnson E; Chapman HD; Nemazee D; Serreze DV. 2004. B cell selection defects underlie the development of diabetogenic APCs in nonobese diabetic mice. J Immunol 172(8):5086-94. [PubMed: 15067092]  [MGI Ref ID J:89156]

Silver K; Bouriez-Jones T; Crockford T; Ferry H; Tang HL; Cyster JG; Cornall RJ. 2006. Spontaneous class switching and B cell hyperactivity increase autoimmunity against intracellular self antigen in Lyn-deficient mice. Eur J Immunol 36(11):2920-7. [PubMed: 17039569]  [MGI Ref ID J:116999]

Silver K; Ferry H; Crockford T; Cornall RJ. 2006. TLR4, TLR9 and MyD88 are not required for the positive selection of autoreactive B cells into the primary repertoire. Eur J Immunol 36(6):1404-12. [PubMed: 16703567]  [MGI Ref ID J:115059]

Zwickey HL; Unternaehrer JJ; Mellman I. 2006. Presentation of self-antigens on MHC class II molecules during dendritic cell maturation. Int Immunol 18(1):199-209. [PubMed: 16361313]  [MGI Ref ID J:104201]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

General Terms and Conditions


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