Former Names NOD.B6-TgN(KLK4mHEL)6Ccg/Dvs (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse
Related Genotype: A/? Tyrc/Tyrc
Tg(KLK4mHEL) was backcrossed from C57BL/6-Tg(KLK4mHEL)6Ccg (stock#002598) onto NOD/Lt in the laboratory of Dr. David Serreze. In 2004 this strain reached N13.
Strains carrying Tg(KLK4mHEL)6Ccg allele
002598 C57BL/6-Tg(KLK4mHEL)6Ccg/JView Strains carrying Tg(KLK4mHEL)6Ccg (1 strain)Strains carrying other alleles of H2-K
023029 B6(C)-H2-Kbm8/LrpJ 000368 B6.C-H2-Kbm1/By 006559 B6.C-H2-Kbm1/ByBir-Gusbmps/BrkJ 000256 B6.C-H2-Kbm1/ByBir-Gusbmps/J 001060 B6.C-H2-Kbm1/ByJ 006558 B6.Cg-H2-Kbm1 Tg(GUSB)4Sly/SndsJ 006877 B6.Cg-Ldlrtm1Her Tg(H2-K-AKR1B1)1Tj/J 017327 B6.Cg-Tg(H2-Kb-NFKB2*)808Hfdg/Mmjax 018055 B6.FVB-Tg(H2-K-S100a9,GFP)1Gabr/J 003240 B6;B10.A-H2a-Tg(H2KmPCC)2939Stoe/J 002432 B6J x B6.C-H2-Kbm1/ByJ-Cdh23v-J/J 003221 BALB/cAnBr-Tg(H2KePCC)2403Stoe/J 011005 C57BL/6-Tg(H2-Kb-Tcra,-Tcrb)P25Ktk/J 005853 NOD.Cg-Tg(HLA-A2/H2-K)1Scr/ShrmJ 017328 STOCK Tg(H2-Kb-NFKB2*)452Hfdg/MmjaxView Strains carrying other alleles of H2-K (15 strains)Strains carrying other alleles of HEL
002599 C57BL/6-Tg(ML5sHEL)5Ccg/J 006610 NOD.B6-Tg(ML5sHEL)5Ccg/DvsJView Strains carrying other alleles of HEL (2 strains)
Currently there is no phenotype information for this strain.
|Allele Name||transgene insertion 6, Christopher C Goodnow|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Common Name(s)||KLK-4; KLK3; mHEL; mHEL-KK;|
|Mutation Made By||Dr. Christopher Goodnow, Stanford University School of Medicine|
|Strain of Origin||C57BL/6JSfd|
|Expressed Gene||HEL, HEL, chicken|
|Promoter||H2-K, histocompatibility 2, K region, mouse, laboratory|
|Molecular Note||A genomic fragment containing the coding exons of the chicken lysozyme gene (also known as hen egg lysozyme, HEL) was linked to the ubiquitously-active mouse H2-Kb promoter and transmembrane sequence (which included the extracellular spacer sequence, transmembrane segment, and cytoplasmic tail). Fluorescence activated cell sorting (FACS) analysis of cells from spleen, lymph node, bone marrow, and thymus from mice carrying the transgene showed membrane expression of chicken lysozyme on these cells. [MGI Ref ID J:78307]|
Akkaraju S; Ho WY; Leong D; Canaan K; Davis MM; Goodnow CC. 1997. A range of CD4 T cell tolerance: partial inactivation to organ-specific antigen allows nondestructive thyroiditis or insulitis. Immunity 7(2):255-71. [PubMed: 9285410] [MGI Ref ID J:78309]
Cyster JG; Goodnow CC. 1995. Protein tyrosine phosphatase 1C negatively regulates antigen receptor signaling in B lymphocytes and determines thresholds for negative selection. Immunity 2(1):13-24. [PubMed: 7600299] [MGI Ref ID J:28348]
Desmarets M; Cadwell CM; Peterson KR; Neades R; Zimring JC. 2009. Minor histocompatibility antigens on transfused leukoreduced units of red blood cells induce bone marrow transplant rejection in a mouse model. Blood 114(11):2315-22. [PubMed: 19525479] [MGI Ref ID J:152426]
Hartley SB; Crosbie J; Brink R; Kantor AB; Basten A; Goodnow CC. 1991. Elimination from peripheral lymphoid tissues of self-reactive B lymphocytes recognizing membrane-bound antigens. Nature 353(6346):765-9. [PubMed: 1944535] [MGI Ref ID J:78307]
Hartley SB; Goodnow CC. 1994. Censoring of self-reactive B cells with a range of receptor affinities in transgenic mice expressing heavy chains for a lysozyme-specific antibody. Int Immunol 6(9):1417-25. [PubMed: 7819151] [MGI Ref ID J:132751]
Hippen KL; Schram BR; Tze LE; Pape KA; Jenkins MK; Behrens TW. 2005. In vivo assessment of the relative contributions of deletion, anergy, and editing to B cell self-tolerance. J Immunol 175(2):909-16. [PubMed: 16002689] [MGI Ref ID J:100671]
Kumar KR; Li L; Yan M; Bhaskarabhatla M; Mobley AB; Nguyen C; Mooney JM; Schatzle JD; Wakeland EK; Mohan C. 2006. Regulation of B cell tolerance by the lupus susceptibility gene Ly108. Science 312(5780):1665-9. [PubMed: 16778059] [MGI Ref ID J:109640]
Liston A; Gray DH; Lesage S; Fletcher AL; Wilson J; Webster KE; Scott HS; Boyd RL; Peltonen L; Goodnow CC. 2004. Gene dosage--limiting role of Aire in thymic expression, clonal deletion, and organ-specific autoimmunity. J Exp Med 200(8):1015-26. [PubMed: 15492124] [MGI Ref ID J:93912]
Liu H; Zheng F; Cao Q; Ren B; Zhu L; Striker G; Vlassara H. 2006. Amelioration of oxidant stress by the defensin lysozyme. Am J Physiol Endocrinol Metab 290(5):E824-32. [PubMed: 16317028] [MGI Ref ID J:108413]
Mecklenbrauker I; Saijo K; Zheng NY; Leitges M; Tarakhovsky A. 2002. Protein kinase Cdelta controls self-antigen-induced B-cell tolerance. Nature 416(6883):860-5. [PubMed: 11976686] [MGI Ref ID J:76134]
Neighbors M; Hartley SB; Xu X; Castro AG; Bouley DM; O'Garra A. 2006. Breakpoints in immunoregulation required for Th1 cells to induce diabetes. Eur J Immunol 36(9):2315-23. [PubMed: 16933361] [MGI Ref ID J:116743]
Phan TG; Amesbury M; Gardam S; Crosbie J; Hasbold J; Hodgkin PD; Basten A; Brink R. 2003. B cell receptor-independent stimuli trigger immunoglobulin (Ig) class switch recombination and production of IgG autoantibodies by anergic self-reactive B cells. J Exp Med 197(7):845-60. [PubMed: 12668643] [MGI Ref ID J:132538]
Schram BR; Tze LE; Ramsey LB; Liu J; Najera L; Vegoe AL; Hardy RR; Hippen KL; Farrar MA; Behrens TW. 2008. B cell receptor Basal signaling regulates antigen-induced Ig light chain rearrangements. J Immunol 180(7):4728-41. [PubMed: 18354197] [MGI Ref ID J:133383]
Silveira PA; Dombrowsky J; Johnson E; Chapman HD; Nemazee D; Serreze DV. 2004. B cell selection defects underlie the development of diabetogenic APCs in nonobese diabetic mice. J Immunol 172(8):5086-94. [PubMed: 15067092] [MGI Ref ID J:89156]
Silver K; Bouriez-Jones T; Crockford T; Ferry H; Tang HL; Cyster JG; Cornall RJ. 2006. Spontaneous class switching and B cell hyperactivity increase autoimmunity against intracellular self antigen in Lyn-deficient mice. Eur J Immunol 36(11):2920-7. [PubMed: 17039569] [MGI Ref ID J:116999]
Silver K; Ferry H; Crockford T; Cornall RJ. 2006. TLR4, TLR9 and MyD88 are not required for the positive selection of autoreactive B cells into the primary repertoire. Eur J Immunol 36(6):1404-12. [PubMed: 16703567] [MGI Ref ID J:115059]
Smith NH; Hod EA; Spitalnik SL; Zimring JC; Hendrickson JE. 2012. Transfusion in the absence of inflammation induces antigen-specific tolerance to murine RBCs. Blood 119(6):1566-9. [PubMed: 22077064] [MGI Ref ID J:181810]
Zwickey HL; Unternaehrer JJ; Mellman I. 2006. Presentation of self-antigens on MHC class II molecules during dendritic cell maturation. Int Immunol 18(1):199-209. [PubMed: 16361313] [MGI Ref ID J:104201]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2525.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $3283.00
Cryorecovery - Standard.
Progeny testing is not required.
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