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Strain Name:

NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+

Stock Number:

004259

Availability:

Research Strain

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General Terms and Conditions
Additional Licensing and Use Restrictions

Genes & Alleles   Rag1;   Rag1tm1Mom;   Tcra;   Tcrb;   Tg(TcraAI4)1Dvs;   Tg(TcrbAI4)1Dvs;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
H2 Haplotypeg7
GenerationF1

Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/Tyrc

Strain Development
This strain is an obligate double hemizygous F1 which is homozygous for Ragtm1Mom. It is made by mating NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs (Stock #004232) with NOD.Cg-Rag1tm1Mom Tg(TcrbAI4)1Dvs (Stock #004233). This strain must be generated in this manner because mice carrying both the alpha and beta transgenes rapidly develop diabetes.

Related Disease (OMIM) Terms

Diabetes Mellitus, Insulin-Dependent; IDDM
Mammalian Phenotype Terms assigned by genotype

Rag1tm1Mom/Rag1tm1Mom Tg(TcraAI4)1Dvs/0 Tg(TcrbAI4)1Dvs/0

        NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs Tg(TcrbAI4)1Dvs
  • immune system phenotype
  • increased susceptibility to autoimmune diabetes (MGI Ref ID J:94192)
    • females have a greatly increased rate of diabetes development compared to non-transgenic NOD controls (100% incidence by 6 weeks of age)

Gene & Allele Details

Allele Symbol Rag1tm1Mom
Allele Name targeted mutation 1, Peter Mombaerts
Common Name(s) RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-;
Mutation Made By Peter Mombaerts,   Max Planck Institute of Biophysics
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Rag1, recombination activating gene 1
Chromosome 2
Gene Common Name(s) MGC43321; RNF74; Rag-1;
Molecular Note A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. [MGI Ref ID J:1934] [MGI Ref ID J:96036]
 
Allele Symbol Tg(TcraAI4)1Dvs
Allele Name transgene insertion 1, David V Serreze
Common Name(s) AI4aTg;
Mutation Made By David Serreze,   The Jackson Laboratory
Strain of OriginNOD/ShiLt
Expressed Gene Tcra, T-cell receptor alpha chain, mouse, laboratory
General Note In conjunction with Rag1tm1Mom
    transgenic mice carrying both the alpha and beta AI4 TCR transgenes exhibit an accelerated development of insulin-dependent diabetes mellitus (IDDM).
    homozygous mice are viable and fertile
Molecular Note The rearranged Tcra transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgene contains a Tcra-V8/Tcra-J cDNA segment. [MGI Ref ID J:93553]
 
Allele Symbol Tg(TcrbAI4)1Dvs
Allele Name transgene insertion 1, David V Serreze
Common Name(s) AI4bTg;
Mutation Made By David Serreze,   The Jackson Laboratory
Strain of OriginNOD/ShiLt
Expressed Gene Tcrb, T-cell receptor beta chain, mouse, laboratory
General Note In conjunction with Rag1tm1Mom
    transgenic mice carrying both the alpha and beta AI4 TCR transgenes exhibit an accelerated development of insulin-dependent diabetes mellitus (IDDM).
    homozygous mice are viable and fertile
Molecular Note The rearranged Tcrb transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgenic construct contains a Tcrb-V2/Tcrb-D/Tcrb-J cDNA segment. [MGI Ref ID J:93553]

Genotyping Protocols

Rag1tm1Mom
Tg(TcrbAI4)1Dvs

Related Strains

View Strains carrying   Rag1tm1Mom     (15 strains)

View Strains carrying   Tg(TcraAI4)1Dvs     (4 strains)

View Strains carrying   Tg(TcrbAI4)1Dvs     (4 strains)

Strains carrying other alleles of Rag1
002194   B6;CBA-Tg(CD3E)26Cpt/J
002506   STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
View Strains carrying other alleles of Rag1     (2 strains)

View Strains carrying other alleles of Tcra     (30 strains)

View Strains carrying other alleles of Tcrb     (31 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Autoimmunity

Research Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)

Tcra related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

Research Tools
Cancer Research (specific T cell deficiency)

Tcrb related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

Rag1tm1Mom related

Cancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)

Hematological Research
Immunological Defects (B and T cell deficiency)

Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Inflammation (B and T cell deficiency)
T Cell Receptor Signaling Defects (B and T cell deficiency)

Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)

Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)

References

Additional References

Price and Supply Information

Strain Name: NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/+ Tg(TcrbAI4)1Dvs/+
Stock Number: 004259

Price Details

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Supply Details

Standard SupplyResearch Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  

General Terms and Conditions

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- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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