Strain Name:

129X1-Smotm1Amc/J

Stock Number:

004288

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129X1-Smohtm1Amc    (Changed: 15-DEC-04 )
Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Andrew P McMahon,   University of Southern California

Description
Mice that are heterozygous for the targeted allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous null mice have an embryonic lethal phenotype, failing to develop past 9.5 days post coitum. Homozygous mutant mice exhibit ventral cyclopia and holoprosencephaly. During development homozygous mice fail to undergo embryonic turning, closure of the ventral midgut and normal rightward looping of the heart. The embryonic heart remains a linear tube. This mutant mouse strain represents a model that may be useful in studies of tumors and neural tube defects due to disruption of the hedgehog pathways.

When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 004526) and a strain expressing Cre recombinase in the skin (Stock No. 004782), this mutant mouse strain may be useful in studies of hedgehog signalling and cell proliferation in the dental epithelium.

When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 004526) and a strain expressing Cre recombinase in the nervous system (Stock No. 003771), this mutant mouse strain may be useful in studies of hedgehog signalling and cerebellar foliation.

When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 004526) and a strain expressing Cre recombinase in the midbrain/dorsal spinal cord (Stock No. 007807), this mutant mouse strain may be useful in studies of craniofacial development.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt a 400 bp region in the first exon of the targeted allele. The construct was electroporated into 129X1/SvJ derived AV3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J derived blastocysts. Chimeric male animals were bred to 129X1/SvJ mice. The chimeric male was bred to a female bearing a Gtrosa26 allele. The alleles were maintained together by the Donating Investigator as a TM/Tg combination for a number of generations by heterozygote X heterozygote crosses. Upon arrival at The Jackson Laboratory, the transgenic allele was eliminated.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Smo
008831   C57BL/6-Tg(Neurod2-Smo*A1)199Jols/J
004526   STOCK Smotm2Amc/J
View Strains carrying other alleles of Smo     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Smotm1Amc/Smotm1Amc

        involves: 129X1/SvJ * C57BL/6
  • embryogenesis phenotype
  • abnormal embryogenesis/ development
    • embryos are identical to Shh/Ihh double mutant embryos   (MGI Ref ID J:128367)
    • abnormal embryo turning
      • at E8.5 embryos are in a primitive, unturned position   (MGI Ref ID J:128367)
    • abnormal vitelline vasculature morphology
      • yolk sac vasculature is rudimentary similar to Shh/Ihh double knock-outs; vascular plexus does not form   (MGI Ref ID J:128367)
      • yolk sacs from E9.5 embryos develop a well-organized plexus of endothelial tubes when Bmp4 is added to culture   (MGI Ref ID J:128367)
  • cardiovascular system phenotype
  • abnormal vitelline vasculature morphology
    • yolk sac vasculature is rudimentary similar to Shh/Ihh double knock-outs; vascular plexus does not form   (MGI Ref ID J:128367)
    • yolk sacs from E9.5 embryos develop a well-organized plexus of endothelial tubes when Bmp4 is added to culture   (MGI Ref ID J:128367)

Smotm1Amc/Smotm1Amc

        involves: 129X1/SvJ * CD-1
  • nervous system phenotype
  • absent neuronal precursor cells
    • floor plate cells (Shh+ Foxa2+), V3 progenitors (Nkx2-2+), and motor neuron progenitors (Olig2+) are absent in the neural tube   (MGI Ref ID J:152259)
  • embryogenesis phenotype
  • embryonic growth retardation
  • growth/size/body phenotype
  • embryonic growth retardation
  • cellular phenotype
  • absent neuronal precursor cells
    • floor plate cells (Shh+ Foxa2+), V3 progenitors (Nkx2-2+), and motor neuron progenitors (Olig2+) are absent in the neural tube   (MGI Ref ID J:152259)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Smotm1Amc/Smotm1Amc Tg(Pdx1-cre)6Tuv/0

        involves: 129X1/SvJ * FVB/N   (conditional)
  • digestive/alimentary phenotype
  • *normal* digestive/alimentary phenotype
    • ventral pancreas and gall bladder development is normal at E10.5   (MGI Ref ID J:151981)

Smotm1Amc/Smotm2Amc Tg(KRT14-cre)1Amc/0

        involves: 129X1/SvJ * C57BL/6 * CBA   (conditional)
  • mortality/aging
  • complete neonatal lethality
    • pups die within 1 day after birth   (MGI Ref ID J:80081)
  • craniofacial phenotype
  • abnormal ameloblast morphology
    • exhibit abnonormally short ameloblasts in the most advanced cusps of first molar region that are overlaid by a scarce, squamous stratum intermedium   (MGI Ref ID J:80081)
    • in incisors, the cuboidal ameloblasts are only 15% of the apical-basal height and contain centrally located round nuclei   (MGI Ref ID J:80081)
    • mitochondria, RER, and Golgi are sparse and evenly distributed in the cytoplasm of incisor ameloblasts and Tomes' processes and the terminal webs do not develop   (MGI Ref ID J:80081)
    • amelolasts exhibit premature withdrawal from the cell cylce   (MGI Ref ID J:80081)
  • abnormal enamel morphology
    • the epithelial enamel organ appears disorganized at the late bell stage in the principal cusps of the first molars   (MGI Ref ID J:80081)
  • abnormal incisor morphology
    • incisors are smaller in diameter and exhibit abnormal folding of the inner dental epithelium   (MGI Ref ID J:80081)
    • incisors show an absence of the papillary layer   (MGI Ref ID J:80081)
  • abnormal molar morphology
    • first and second molars of both the maxilla and mandible are abnormally fused, forming a single gigantic anlage   (MGI Ref ID J:80081)
    • dental cord is virtually absent   (MGI Ref ID J:80081)
    • the stellate reticulum is hypocellular and shows absence of early vascular loops in the coronal aspect   (MGI Ref ID J:80081)
    • the outer dental epithelium forms a continuous layer without the gaps seen in controls   (MGI Ref ID J:80081)
    • abnormal molar crown morphology
      • cusps of first molars are shallow, broad, underdeveloped and misshapen   (MGI Ref ID J:80081)
  • growth/size/body phenotype
  • abnormal ameloblast morphology
    • exhibit abnonormally short ameloblasts in the most advanced cusps of first molar region that are overlaid by a scarce, squamous stratum intermedium   (MGI Ref ID J:80081)
    • in incisors, the cuboidal ameloblasts are only 15% of the apical-basal height and contain centrally located round nuclei   (MGI Ref ID J:80081)
    • mitochondria, RER, and Golgi are sparse and evenly distributed in the cytoplasm of incisor ameloblasts and Tomes' processes and the terminal webs do not develop   (MGI Ref ID J:80081)
    • amelolasts exhibit premature withdrawal from the cell cylce   (MGI Ref ID J:80081)
  • abnormal enamel morphology
    • the epithelial enamel organ appears disorganized at the late bell stage in the principal cusps of the first molars   (MGI Ref ID J:80081)
  • abnormal incisor morphology
    • incisors are smaller in diameter and exhibit abnormal folding of the inner dental epithelium   (MGI Ref ID J:80081)
    • incisors show an absence of the papillary layer   (MGI Ref ID J:80081)
  • abnormal molar morphology
    • first and second molars of both the maxilla and mandible are abnormally fused, forming a single gigantic anlage   (MGI Ref ID J:80081)
    • dental cord is virtually absent   (MGI Ref ID J:80081)
    • the stellate reticulum is hypocellular and shows absence of early vascular loops in the coronal aspect   (MGI Ref ID J:80081)
    • the outer dental epithelium forms a continuous layer without the gaps seen in controls   (MGI Ref ID J:80081)
    • abnormal molar crown morphology
      • cusps of first molars are shallow, broad, underdeveloped and misshapen   (MGI Ref ID J:80081)

Smotm1Amc/Smotm2Amc Tg(Wnt1-cre)11Rth/?

        involves: C57BL/6J * CBA/J   (conditional)
  • craniofacial phenotype
  • abnormal craniofacial development   (MGI Ref ID J:89445)
    • abnormal Meckel's cartilage morphology
      • hypoplastic and short   (MGI Ref ID J:89445)
    • absent Reichert cartilage
      • the basi- cerato- and thyro hyoid elements are missing   (MGI Ref ID J:89445)
  • abnormal cranium morphology   (MGI Ref ID J:89445)
    • abnormal ethmoidal bone morphology
      • mesethmoid bone is missing   (MGI Ref ID J:89445)
    • abnormal lacrimal bone morphology
      • absent or appears as a tiny fragment   (MGI Ref ID J:89445)
    • abnormal mandible morphology
      • the dentate is reduced in length but the lamina is thicker   (MGI Ref ID J:89445)
      • at E9.5, there is an increase in apoptotic cells along the midline   (MGI Ref ID J:89445)
      • at E10.5, apoptotic cells are observed along the midline and laterally   (MGI Ref ID J:89445)
      • at E10.5, mandibles are 9% shorter than the wild-type and only undergoes a 1.5-fold along the dorsal-ventral axis compared to a 4-fold expansion in wild-type   (MGI Ref ID J:89445)
      • at E11.5, cell proliferation is decreased   (MGI Ref ID J:89445)
      • abnormal mandibular condyloid process morphology
        • mice have an extra condylar process   (MGI Ref ID J:89445)
    • abnormal maxilla morphology
      • premaxilla and maxilla retain their lateral-most parts only   (MGI Ref ID J:89445)
    • abnormal sphenoid bone morphology   (MGI Ref ID J:89445)
      • abnormal basisphenoid bone morphology
        • rostral half is missing   (MGI Ref ID J:89445)
      • abnormal orbitosphenoid bone morphology
      • absent presphenoid bone   (MGI Ref ID J:89445)
      • absent pterygoid process   (MGI Ref ID J:89445)
      • small alisphenoid bone
    • abnormal temporal bone morphology   (MGI Ref ID J:89445)
      • abnormal squamosal bone morphology
      • absent styloid process   (MGI Ref ID J:89445)
    • absent palatine bone   (MGI Ref ID J:89445)
    • absent vomer bone   (MGI Ref ID J:89445)
    • short zygomatic bone
    • small nasal bone
      • nasal bone is hypoplastic   (MGI Ref ID J:89445)
  • abnormal nasal septum morphology
    • the nasal septum is incomplete   (MGI Ref ID J:89445)
  • abnormal tooth morphology
    • teeth are malformed and arrested   (MGI Ref ID J:89445)
    • absent lower incisors   (MGI Ref ID J:89445)
  • absent gonial bone   (MGI Ref ID J:89445)
  • absent incus   (MGI Ref ID J:89445)
  • absent malleus   (MGI Ref ID J:89445)
  • absent middle ear ossicles   (MGI Ref ID J:89445)
  • absent stapes   (MGI Ref ID J:89445)
  • absent tongue   (MGI Ref ID J:89445)
  • hearing/vestibular/ear phenotype
  • absent gonial bone   (MGI Ref ID J:89445)
  • absent incus   (MGI Ref ID J:89445)
  • absent malleus   (MGI Ref ID J:89445)
  • absent middle ear ossicles   (MGI Ref ID J:89445)
  • absent stapes   (MGI Ref ID J:89445)
  • absent tympanic ring   (MGI Ref ID J:89445)
  • digestive/alimentary phenotype
  • absent tongue   (MGI Ref ID J:89445)
  • respiratory system phenotype
  • abnormal nasal septum morphology
    • the nasal septum is incomplete   (MGI Ref ID J:89445)
  • abnormal thyroid cartilage morphology   (MGI Ref ID J:89445)
  • skeleton phenotype
  • abnormal Meckel's cartilage morphology
    • hypoplastic and short   (MGI Ref ID J:89445)
  • abnormal cranium morphology   (MGI Ref ID J:89445)
    • abnormal ethmoidal bone morphology
      • mesethmoid bone is missing   (MGI Ref ID J:89445)
    • abnormal lacrimal bone morphology
      • absent or appears as a tiny fragment   (MGI Ref ID J:89445)
    • abnormal mandible morphology
      • the dentate is reduced in length but the lamina is thicker   (MGI Ref ID J:89445)
      • at E9.5, there is an increase in apoptotic cells along the midline   (MGI Ref ID J:89445)
      • at E10.5, apoptotic cells are observed along the midline and laterally   (MGI Ref ID J:89445)
      • at E10.5, mandibles are 9% shorter than the wild-type and only undergoes a 1.5-fold along the dorsal-ventral axis compared to a 4-fold expansion in wild-type   (MGI Ref ID J:89445)
      • at E11.5, cell proliferation is decreased   (MGI Ref ID J:89445)
      • abnormal mandibular condyloid process morphology
        • mice have an extra condylar process   (MGI Ref ID J:89445)
    • abnormal maxilla morphology
      • premaxilla and maxilla retain their lateral-most parts only   (MGI Ref ID J:89445)
    • abnormal sphenoid bone morphology   (MGI Ref ID J:89445)
      • abnormal basisphenoid bone morphology
        • rostral half is missing   (MGI Ref ID J:89445)
      • abnormal orbitosphenoid bone morphology
      • absent presphenoid bone   (MGI Ref ID J:89445)
      • absent pterygoid process   (MGI Ref ID J:89445)
      • small alisphenoid bone
    • abnormal temporal bone morphology   (MGI Ref ID J:89445)
      • abnormal squamosal bone morphology
      • absent styloid process   (MGI Ref ID J:89445)
    • absent palatine bone   (MGI Ref ID J:89445)
    • absent vomer bone   (MGI Ref ID J:89445)
    • short zygomatic bone
    • small nasal bone
      • nasal bone is hypoplastic   (MGI Ref ID J:89445)
  • abnormal thyroid cartilage morphology   (MGI Ref ID J:89445)
  • absent Reichert cartilage
    • the basi- cerato- and thyro hyoid elements are missing   (MGI Ref ID J:89445)
  • absent gonial bone   (MGI Ref ID J:89445)
  • absent incus   (MGI Ref ID J:89445)
  • absent malleus   (MGI Ref ID J:89445)
  • absent middle ear ossicles   (MGI Ref ID J:89445)
  • absent stapes   (MGI Ref ID J:89445)
  • vision/eye phenotype
  • abnormal orbitosphenoid bone morphology
  • growth/size/body phenotype
  • abnormal nasal septum morphology
    • the nasal septum is incomplete   (MGI Ref ID J:89445)
  • abnormal tooth morphology
    • teeth are malformed and arrested   (MGI Ref ID J:89445)
    • absent lower incisors   (MGI Ref ID J:89445)
  • absent tongue   (MGI Ref ID J:89445)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Smotm1Amc related

Developmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects
      heart
Neural Tube Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Smotm1Amc
Allele Name targeted mutation 1, Andrew P McMahon
Allele Type Targeted (Null/Knockout)
Common Name(s) Smo-; Smon; Smonull; Smorec;
Mutation Made By Andrew McMahon,   University of Southern California
Strain of Origin129X1/SvJ
ES Cell Line NameAV3
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Smo, smoothened homolog (Drosophila)
Chromosome 6
Gene Common Name(s) E130215L21Rik; FZD11; Gx; RIKEN cDNA E130215L21 gene; SMOH; bent body; bnb;
Molecular Note An frt-flanked neomycin resistance cassette replaced a segment containing 44 bp upstream and 358 bp downstream of the translation initiation codon. [MGI Ref ID J:175111]

Genotyping

Genotyping Information

Genotyping Protocols

Smotm1Amc, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Smotm1Amc related

Allen BL; Tenzen T; McMahon AP. 2007. The Hedgehog-binding proteins Gas1 and Cdo cooperate to positively regulate Shh signaling during mouse development. Genes Dev 21(10):1244-57. [PubMed: 17504941]  [MGI Ref ID J:121553]

Astorga J; Carlsson P. 2007. Hedgehog induction of murine vasculogenesis is mediated by Foxf1 and Bmp4. Development 134(20):3753-61. [PubMed: 17881493]  [MGI Ref ID J:128367]

Blaess S; Corrales JD; Joyner AL. 2006. Sonic hedgehog regulates Gli activator and repressor functions with spatial and temporal precision in the mid/hindbrain region. Development 133(9):1799-809. [PubMed: 16571630]  [MGI Ref ID J:108509]

Bouldin CM; Gritli-Linde A; Ahn S; Harfe BD. 2010. Shh pathway activation is present and required within the vertebrate limb bud apical ectodermal ridge for normal autopod patterning. Proc Natl Acad Sci U S A 107(12):5489-94. [PubMed: 20212115]  [MGI Ref ID J:158689]

Cain JE; Islam E; Haxho F; Blake J; Rosenblum ND. 2011. GLI3 repressor controls functional development of the mouse ureter. J Clin Invest 121(3):1199-206. [PubMed: 21339645]  [MGI Ref ID J:172032]

Cain JE; Islam E; Haxho F; Chen L; Bridgewater D; Nieuwenhuis E; Hui CC; Rosenblum ND. 2009. GLI3 repressor controls nephron number via regulation of Wnt11 and Ret in ureteric tip cells. PLoS One 4(10):e7313. [PubMed: 19809516]  [MGI Ref ID J:154102]

Chamberlain CE; Jeong J; Guo C; Allen BL; McMahon AP. 2008. Notochord-derived Shh concentrates in close association with the apically positioned basal body in neural target cells and forms a dynamic gradient during neural patterning. Development 135(6):1097-106. [PubMed: 18272593]  [MGI Ref ID J:132152]

Charron F; Stein E; Jeong J; McMahon AP; Tessier-Lavigne M. 2003. The Morphogen Sonic Hedgehog Is an Axonal Chemoattractant that Collaborates with Netrin-1 in Midline Axon Guidance. Cell 113(1):11-23. [PubMed: 12679031]  [MGI Ref ID J:82736]

Chen MH; Wilson CW; Li YJ; Law KK; Lu CS; Gacayan R; Zhang X; Hui CC; Chuang PT. 2009. Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved. Genes Dev 23(16):1910-28. [PubMed: 19684112]  [MGI Ref ID J:151923]

Cheung HO; Zhang X; Ribeiro A; Mo R; Makino S; Puviindran V; Law KK; Briscoe J; Hui CC. 2009. The kinesin protein Kif7 is a critical regulator of Gli transcription factors in mammalian hedgehog signaling. Sci Signal 2(76):ra29. [PubMed: 19549984]  [MGI Ref ID J:152259]

Cho A; Ko HW; Eggenschwiler JT. 2008. FKBP8 cell-autonomously controls neural tube patterning through a Gli2- and Kif3a-dependent mechanism. Dev Biol 321(1):27-39. [PubMed: 18590716]  [MGI Ref ID J:138713]

Cooper CL; Hardy RR; Reth M; Desiderio S. 2012. Non-cell-autonomous hedgehog signaling promotes murine B lymphopoiesis from hematopoietic progenitors. Blood 119(23):5438-48. [PubMed: 22517907]  [MGI Ref ID J:188661]

Corrales JD; Blaess S; Mahoney EM; Joyner AL. 2006. The level of sonic hedgehog signaling regulates the complexity of cerebellar foliation. Development 133(9):1811-21. [PubMed: 16571625]  [MGI Ref ID J:108507]

Cui C; Chatterjee B; Lozito TP; Zhang Z; Francis RJ; Yagi H; Swanhart LM; Sanker S; Francis D; Yu Q; San Agustin JT; Puligilla C; Chatterjee T; Tansey T; Liu X; Kelley MW; Spiliotis ET; Kwiatkowski AV; Tuan R; Pazour GJ; Hukriede NA; Lo CW. 2013. Wdpcp, a PCP protein required for ciliogenesis, regulates directional cell migration and cell polarity by direct modulation of the actin cytoskeleton. PLoS Biol 11(11):e1001720. [PubMed: 24302887]  [MGI Ref ID J:205269]

Dakubo GD; Mazerolle CJ; Wallace VA. 2006. Expression of Notch and Wnt pathway components and activation of Notch signaling in medulloblastomas from heterozygous patched mice. J Neurooncol 79(3):221-7. [PubMed: 16598417]  [MGI Ref ID J:115750]

Eggenschwiler JT; Bulgakov OV; Qin J; Li T; Anderson KV. 2006. Mouse Rab23 regulates Hedgehog signaling from Smoothened to Gli proteins. Dev Biol 290(1):1-12. [PubMed: 16364285]  [MGI Ref ID J:104803]

El Andaloussi A; Graves S; Meng F; Mandal M; Mashayekhi M; Aifantis I. 2006. Hedgehog signaling controls thymocyte progenitor homeostasis and differentiation in the thymus. Nat Immunol 7(4):418-26. [PubMed: 16518394]  [MGI Ref ID J:112555]

Fogel JL; Chiang C; Huang X; Agarwala S. 2008. Ventral specification and perturbed boundary formation in the mouse midbrain in the absence of Hedgehog signaling. Dev Dyn 237(5):1359-72. [PubMed: 18429041]  [MGI Ref ID J:134674]

Gritli-Linde A; Bei M; Maas R; Zhang XM; Linde A; McMahon AP. 2002. Shh signaling within the dental epithelium is necessary for cell proliferation, growth and polarization. Development 129(23):5323-37. [PubMed: 12403705]  [MGI Ref ID J:80081]

Gritli-Linde A; Hallberg K; Harfe BD; Reyahi A; Kannius-Janson M; Nilsson J; Cobourne MT; Sharpe PT; McMahon AP; Linde A. 2007. Abnormal hair development and apparent follicular transformation to mammary gland in the absence of hedgehog signaling. Dev Cell 12(1):99-112. [PubMed: 17199044]  [MGI Ref ID J:117334]

Hammond R; Blaess S; Abeliovich A. 2009. Sonic hedgehog is a chemoattractant for midbrain dopaminergic axons. PLoS One 4(9):e7007. [PubMed: 19774071]  [MGI Ref ID J:153607]

Hayashi S; Tenzen T; McMahon AP. 2003. Maternal inheritance of Cre activity in a Sox2Cre deleter strain. Genesis 37(2):51-3. [PubMed: 14595839]  [MGI Ref ID J:86588]

Hayes L; Ralls S; Wang H; Ahn S. 2013. Duration of Shh signaling contributes to mDA neuron diversity. Dev Biol 374(1):115-26. [PubMed: 23201023]  [MGI Ref ID J:193109]

Hilton MJ; Tu X; Long F. 2007. Tamoxifen-inducible gene deletion reveals a distinct cell type associated with trabecular bone, and direct regulation of PTHrP expression and chondrocyte morphology by Ihh in growth region cartilage. Dev Biol 308(1):93-105. [PubMed: 17560974]  [MGI Ref ID J:123971]

Hu JK; McGlinn E; Harfe BD; Kardon G; Tabin CJ. 2012. Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev 26(18):2088-102. [PubMed: 22987639]  [MGI Ref ID J:187741]

Jeong J; Mao J; Tenzen T; Kottmann AH; McMahon AP. 2004. Hedgehog signaling in the neural crest cells regulates the patterning and growth of facial primordia. Genes Dev 18(8):937-51. [PubMed: 15107405]  [MGI Ref ID J:89445]

Komada M; Saitsu H; Kinboshi M; Miura T; Shiota K; Ishibashi M. 2008. Hedgehog signaling is involved in development of the neocortex. Development 135(16):2717-27. [PubMed: 18614579]  [MGI Ref ID J:138572]

Lau J; Hebrok M. 2010. Hedgehog signaling in pancreas epithelium regulates embryonic organ formation and adult beta-cell function. Diabetes 59(5):1211-21. [PubMed: 20185815]  [MGI Ref ID J:164411]

Lillien L; Gulacsi A. 2006. Environmental signals elicit multiple responses in dorsal telencephalic progenitors by threshold-dependent mechanisms. Cereb Cortex 16 Suppl 1:i74-81. [PubMed: 16766711]  [MGI Ref ID J:174485]

Lin L; Bu L; Cai CL; Zhang X; Evans S. 2006. Isl1 is upstream of sonic hedgehog in a pathway required for cardiac morphogenesis. Dev Biol 295(2):756-63. [PubMed: 16687132]  [MGI Ref ID J:110602]

Long F; Chung UI; Ohba S; McMahon J; Kronenberg HM; McMahon AP. 2004. Ihh signaling is directly required for the osteoblast lineage in the endochondral skeleton. Development 131(6):1309-18. [PubMed: 14973297]  [MGI Ref ID J:88635]

Long F; Joeng KS; Xuan S; Efstratiadis A; McMahon AP. 2006. Independent regulation of skeletal growth by Ihh and IGF signaling. Dev Biol 298(1):327-33. [PubMed: 16905129]  [MGI Ref ID J:119575]

Long F; Zhang XM; Karp S; Yang Y; McMahon AP. 2001. Genetic manipulation of hedgehog signaling in the endochondral skeleton reveals a direct role in the regulation of chondrocyte proliferation. Development 128(24):5099-108. [PubMed: 11748145]  [MGI Ref ID J:73071]

Machold R; Hayashi S; Rutlin M; Muzumdar MD; Nery S; Corbin JG; Gritli-Linde A; Dellovade T; Porter JA; Rubin LL; Dudek H; McMahon AP; Fishell G. 2003. Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches. Neuron 39(6):937-50. [PubMed: 12971894]  [MGI Ref ID J:85603]

Mukhopadhyay S; Wen X; Ratti N; Loktev A; Rangell L; Scales SJ; Jackson PK. 2013. The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling. Cell 152(1-2):210-23. [PubMed: 23332756]  [MGI Ref ID J:193395]

Norman RX; Ko HW; Huang V; Eun CM; Abler LL; Zhang Z; Sun X; Eggenschwiler JT. 2009. Tubby-like protein 3 (TULP3) regulates patterning in the mouse embryo through inhibition of Hedgehog signaling. Hum Mol Genet 18(10):1740-54. [PubMed: 19286674]  [MGI Ref ID J:147574]

Patterson VL; Damrau C; Paudyal A; Reeve B; Grimes DT; Stewart ME; Williams DJ; Siggers P; Greenfield A; Murdoch JN. 2009. Mouse hitchhiker mutants have spina bifida, dorso-ventral patterning defects and polydactyly: identification of Tulp3 as a novel negative regulator of the Sonic hedgehog pathway. Hum Mol Genet 18(10):1719-39. [PubMed: 19223390]  [MGI Ref ID J:147584]

Qin J; Lin Y; Norman RX; Ko HW; Eggenschwiler JT. 2011. Intraflagellar transport protein 122 antagonizes Sonic Hedgehog signaling and controls ciliary localization of pathway components. Proc Natl Acad Sci U S A 108(4):1456-61. [PubMed: 21209331]  [MGI Ref ID J:168317]

Reiter JF; Skarnes WC. 2006. Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibition. Genes Dev 20(1):22-7. [PubMed: 16357211]  [MGI Ref ID J:103820]

Rice R; Spencer-Dene B; Connor EC; Gritli-Linde A; McMahon AP; Dickson C; Thesleff I; Rice DP. 2004. Disruption of Fgf10/Fgfr2b-coordinated epithelial-mesenchymal interactions causes cleft palate. J Clin Invest 113(12):1692-700. [PubMed: 15199404]  [MGI Ref ID J:90909]

Rodda SJ; McMahon AP. 2006. Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors. Development 133(16):3231-44. [PubMed: 16854976]  [MGI Ref ID J:114494]

Spence JR; Lange AW; Lin SC; Kaestner KH; Lowy AM; Kim I; Whitsett JA; Wells JM. 2009. Sox17 regulates organ lineage segregation of ventral foregut progenitor cells. Dev Cell 17(1):62-74. [PubMed: 19619492]  [MGI Ref ID J:151981]

Vincent SD; Mayeuf A; Niro C; Saitou M; Buckingham M. 2012. Non Conservation of Function for the Evolutionarily Conserved Prdm1 Protein in the Control of the Slow Twitch Myogenic Program in the Mouse Embryo. Mol Biol Evol :. [PubMed: 22522309]  [MGI Ref ID J:186268]

Vokes SA; Yatskievych TA; Heimark RL; McMahon J; McMahon AP; Antin PB; Krieg PA. 2004. Hedgehog signaling is essential for endothelial tube formation during vasculogenesis. Development 131(17):4371-80. [PubMed: 15294868]  [MGI Ref ID J:92048]

Warr N; Powles-Glover N; Chappell A; Robson J; Norris D; Arkell RM. 2008. Zic2-associated holoprosencephaly is caused by a transient defect in the organizer region during gastrulation. Hum Mol Genet 17(19):2986-96. [PubMed: 18617531]  [MGI Ref ID J:138862]

Wijgerde M; McMahon JA; Rule M; McMahon AP. 2002. A direct requirement for Hedgehog signaling for normal specification of all ventral progenitor domains in the presumptive mammalian spinal cord. Genes Dev 16(22):2849-64. [PubMed: 12435628]  [MGI Ref ID J:80206]

Yam PT; Kent CB; Morin S; Farmer WT; Alchini R; Lepelletier L; Colman DR; Tessier-Lavigne M; Fournier AE; Charron F. 2012. 14-3-3 proteins regulate a cell-intrinsic switch from sonic hedgehog-mediated commissural axon attraction to repulsion after midline crossing. Neuron 76(4):735-49. [PubMed: 23177959]  [MGI Ref ID J:196985]

Zhang XM; Ramalho-Santos M; McMahon AP. 2001. Smoothened mutants reveal redundant roles for Shh and Ihh signaling including regulation of L/R symmetry by the mouse node. Cell 106(2):781-92. [PubMed: 11517919]  [MGI Ref ID J:175111]

Zhao L; Saitsu H; Sun X; Shiota K; Ishibashi M. 2010. Sonic hedgehog is involved in formation of the ventral optic cup by limiting Bmp4 expression to the dorsal domain. Mech Dev 127(1-2):62-72. [PubMed: 19854269]  [MGI Ref ID J:156737]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a 129 background, and is maintained on the same. Upon arrival at The Jackson Laboratory, the transgenic allele was eliminated. Expected coat color is: White Bellied Agouti

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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