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Strain Name:

NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ

Stock Number:

004291

Availability:

Repository-Cryopreserved

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Genes & Alleles   Il10;   Il10tm1Cgn;   Il4;   Il4tm1Cgn;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Background Strain NOD/ShiLt
Donor Strain 129
Donating Investigator David Serreze,   The Jackson Laboratory
H2 Haplotypeg7
GenerationN2F+1p

Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/Tyrc

Strain Description
Mice homozygous for the Il10 and Il4 targeted mutations are viable and fertile when housed under SPF conditions. NOD/Lt mice deficient for both of these genes develop type 1 diabetes at a rate equivalent to the parental strain. The Il10 tm1Cgn mutation also renders this NOD/Lt stock susceptible to colitis (although not as severe as other strains of Il10 deficient mice) when maintained under standard housing conditions.

Strain Development
For details related to the construction of each allele, please refer to the entries for strains 004266 and 004222.

Gene & Allele Details

Allele Symbol Il10tm1Cgn
Allele Name targeted mutation 1, University of Cologne
Common Name(s) IL-10 KO; IL-10-; IL-10KO; IL-10KO; Il10-;
Mutation Made By Ralf Kuhn,   University of Cologne
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Il10, interleukin 10
Chromosome 1
Gene Common Name(s) CSIF; IL-10; IL10A; IL10X; Il-10; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor;
Molecular Note A 500 bp genomic fragment containing codons 5-55 was replaced with a linker containing a termination codon followed by a neomycin cassette. A termination codon was also introduced into exon 3. No IL10 activity was detectable in ELISA assays in supernatants of in vitro splenic T cells derived from homozygous mice.
 
Allele Symbol Il4tm1Cgn
Allele Name targeted mutation 1, University of Cologne
Common Name(s) IL-4 KO; IL-4-; IL-4KO; IL-4T-; IL4-; IL4tm1cgn129; IL4T;
Mutation Made By Ralf Kuhn,   University of Cologne
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Il4, interleukin 4
Chromosome 11
Gene Common Name(s) BCGF-1; BCGF1; BSF1; IL-4; Il-4; Il4e12; MGC79402;
Molecular Note A translational stop codon and a neomycin resistance gene were inserted into the first exon of the gene. [MGI Ref ID J:704]

Control Information

  Control
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Il10tm1Cgn
Il4tm1Cgn

Colony Maintenance

Breeding & HusbandryThis stock was created by Dr. David Serreze, The Jackson Laboratory, Bar Harbor, by intercrossing NOD/Lt mice congenic for the Il4tm1Cgn and Il10tm1Cgn mutations. An intercross strategy was then employed to fix the targeted mutations to homozygosity. The stock is at the N8 backcross generation, and has been confirmed by genome scan to be homozygous for linkage markers delineating twenty-three previously identified, insulin dependent diabetes susceptibility loci of NOD/Lt origin.

Related Strains

View Strains carrying   Il10tm1Cgn     (10 strains)

Strains carrying   Il4tm1Cgn allele
002253   B6.129P2-Il4tm1Cgn/J
005879   D1Lac.Cg-Il4tm1Cgn/J
002574   NOD.129P2(B6)-Il4tm1Cgn/Dvs
004222   NOD.129P2(B6)-Il4tm1Cgn/DvsJ
004228   NOD.Cg-Il10tm1Cgn Il4tm1Cgn/Dvs
View Strains carrying   Il4tm1Cgn     (5 strains)

Strains carrying other alleles of Il4
002496   BALB/c-Il4tm2Nnt/J
004190   C.129-Il4tm1Lky/J
003480   C.129S2(B6)-Il4tm1Gru/J
002518   C57BL/6-Il4tm1Nnt/J
002230   C57BL/6J-Tg(LckIl4)1315Dbl/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
View Strains carrying other alleles of Il4     (6 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Congenics with Mutations Affecting Cytokine Production by Autoreactive T Cells)

Il10tm1Cgn related

Cancer Research
Growth Factors/Receptors/Cytokines

Hematological Research
Anemia, Iron Deficiency and Transport Defects (hemolytic)
Immunological Defects

Immunology and Inflammation Research
Autoimmunity
Growth Factors/Receptors/Cytokines
Immunodeficiency
Inflammation (Inflammatory bowel disease)

Il4tm1Cgn related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines

References

Selected Reference(s)

Serreze DV; Chapman HD; Post CM; Johnson EA; Suarez-Pinzon WL; Rabinovitch A. 2001. Th1 to Th2 cytokine shifts in nonobese diabetic mice: sometimes an outcome, rather than the cause, of diabetes resistance elicited by immunostimulation. J Immunol 166(2):1352-9. [PubMed: 11145720]  [MGI Ref ID J:109883]

Additional References

Price and Supply Information

Strain Name: NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ
Stock Number: 004291

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Type 1 Diabetes Repository collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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