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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 18-MAY-09 Species laboratory mouse Background Strain C57BL/6J Donor Strain CXB-1/By Generation N5F15 (20-DEC-04) Appearance
black
Related Genotype: a/a
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Pde6b
View Strains carrying other alleles of Pde6b (82 strains)
Phenotype
Phenotype Information
Retinitis Pigmentosa; RP - Models with phenotypic similarity to human disease where etiologies are distinct.2
2 Human genes are associated with this disease. Orthologs of those genes do not appear in the mouse genotype(s).
assigned by genotype
Pde6brd10/Pde6brd10
B6.CXB1-Pde6brd10/J
- vision/eye phenotype
- *normal* vision/eye phenotype (MGI Ref ID J:122722)
- abnormal eye electrophysiology (MGI Ref ID J:122722)
- mice showed reduced rod and cone responses under both dark- and light-adapted conditions compared to wild-type
- a small a-wave appeared only at the brightest flash intensity indicating loss of sensitivity in dark-adapted, 30 day old mice
- the loss of cone function was not registered as fast by b-wave response from light-adapted compared to dark-adapted mice
- ERG a- and b- wave amplitudes are greater in mice treated with tauroursodeoxycholic acid
- abnormal retinal rod cell morphology (MGI Ref ID J:122722)
- retinal rod cell degeneration (MGI Ref ID J:122722)
- rod photoreceptor cells start degenerating in the central retina at 16 days of age and in the peripheral retina at 20 days of age
- by 60 days of age no photoreceptors remain
- abnormal retinal vasculature (MGI Ref ID J:122722)
- sclerotic retinal vessels are observed at 4 weeks of age
- retinal degeneration (MGI Ref ID J:122722)
- clinical abnormalities are observable at 4 weeks of age
- histological analyses show progressive retinal outer nuclear layer degeneration (ONL) beginning in the center at 16 days of age and spreading to the periphery by 20 days of age
- by 60 days of age no ONL remains
- nuclei counts in the ONL over time reflects progressive degeneration; inner nuclear layers are not affected
- dark-reared mice showed no degeneration until 24 days of age with initial nuclei loss apparent at 30 days
- tauroursodeoxycholic acid slows onset of degeneration of retinal outer nuclear layers and photoreceptors
- retinal outer nuclear layer degeneration (MGI Ref ID J:122722)
- histological analyses show progressive retinal outer nuclear layer degeneration (ONL) beginning in the center at 16 days of age and spreading to the periphery by 20 days of age
- cardiovascular system phenotype
- abnormal retinal vasculature (MGI Ref ID J:122722)
- sclerotic retinal vessels are observed at 4 weeks of age
- nervous system phenotype
- abnormal retinal rod cell morphology (MGI Ref ID J:122722)
- retinal rod cell degeneration (MGI Ref ID J:122722)
- rod photoreceptor cells start degenerating in the central retina at 16 days of age and in the peripheral retina at 20 days of age
- by 60 days of age no photoreceptors remain
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Pde6brd10 | ||
|---|---|---|---|
| Allele Name | retinal degeneration 10 | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | Pde6brd1-J; rd10; | ||
| Strain of Origin | CXB1/ByJ | ||
| Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CSNB3; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10; | ||
| Molecular Note | An initial complementation test with Pde6b rd1 demonstrated allelism. Sequence analysis shows that the rd10 mutation is caused by a missense mutation in exon 13, which encodes the beta subunit of the protein. [MGI Ref ID J:75095] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Pde6brd10 relatedBarhoum R; Martinez-Navarrete G; Corrochano S; Germain F; Fernandez-Sanchez L; de la Rosa EJ; de la Villa P; Cuenca N. 2008. Functional and structural modifications during retinal degeneration in the rd10 mouse. Neuroscience 155(3):698-713. [PubMed: 18639614] [MGI Ref ID J:140868]
Boatright JH; Moring AG; McElroy C; Phillips MJ; Do VT; Chang B; Hawes NL; Boyd AP; Sidney SS; Stewart RE; Minear SC; Chaudhury R; Ciavatta VT; Rodrigues CM; Steer CJ; Nickerson JM; Pardue MT. 2006. Tool from ancient pharmacopoeia prevents vision loss. Mol Vis 12:1706-14. [PubMed: 17213800] [MGI Ref ID J:117305]
Chang B; Hawes NL; Hurd RE; Davisson MT; Nusinowitz S; Heckenlively JR. 2002. Retinal degeneration mutants in the mouse. Vision Res 42(4):517-25. [PubMed: 11853768] [MGI Ref ID J:75095]
Chang B; Hawes NL; Pardue MT; German AM; Hurd RE; Davisson MT; Nusinowitz S; Rengarajan K; Boyd AP; Sidney SS; Phillips MJ; Stewart RE; Chaudhury R; Nickerson JM; Heckenlively JR; Boatright JH. 2007. Two mouse retinal degenerations caused by missense mutations in the beta-subunit of rod cGMP phosphodiesterase gene. Vision Res 47(5):624-33. [PubMed: 17267005] [MGI Ref ID J:122722]
Corrochano S; Barhoum R; Boya P; Arroba AI; Rodriguez-Muela N; Gomez-Vicente V; Bosch F; de Pablo F; de la Villa P; de la Rosa EJ. 2008. Attenuation of vision loss and delay in apoptosis of photoreceptors induced by proinsulin in a mouse model of retinitis pigmentosa. Invest Ophthalmol Vis Sci 49(9):4188-94. [PubMed: 18515565] [MGI Ref ID J:141803]
Deleon E; Lederman M; Berenstein E; Meir T; Chevion M; Chowers I. 2009. Alteration in iron metabolism during retinal degeneration in rd10 mouse. Invest Ophthalmol Vis Sci 50(3):1360-5. [PubMed: 18997094] [MGI Ref ID J:146676]
Gargini C; Terzibasi E; Mazzoni F; Strettoi E. 2007. Retinal organization in the retinal degeneration 10 (rd10) mutant mouse: a morphological and ERG study. J Comp Neurol 500(2):222-38. [PubMed: 17111372] [MGI Ref ID J:132845]
Mazzoni F; Novelli E; Strettoi E. 2008. Retinal ganglion cells survive and maintain normal dendritic morphology in a mouse model of inherited photoreceptor degeneration. J Neurosci 28(52):14282-92. [PubMed: 19109509] [MGI Ref ID J:143877]
Pang JJ; Boye SL; Kumar A; Dinculescu A; Deng W; Li J; Li Q; Rani A; Foster TC; Chang B; Hawes NL; Boatright JH; Hauswirth WW. 2008. AAV-mediated gene therapy for retinal degeneration in the rd10 mouse containing a recessive PDEbeta mutation. Invest Ophthalmol Vis Sci 49(10):4278-83. [PubMed: 18586879] [MGI Ref ID J:140150]
Phillips MJ; Walker TA; Choi HY; Faulkner AE; Kim MK; Sidney SS; Boyd AP; Nickerson JM; Boatright JH; Pardue MT. 2008. Tauroursodeoxycholic acid preservation of photoreceptor structure and function in the rd10 mouse through postnatal day 30. Invest Ophthalmol Vis Sci 49(5):2148-55. [PubMed: 18436848] [MGI Ref ID J:135201]
Puthussery T; Gayet-Primo J; Pandey S; Duvoisin RM; Taylor WR. 2009. Differential loss and preservation of glutamate receptor function in bipolar cells in the rd10 mouse model of retinitis pigmentosa. Eur J Neurosci 29(8):1533-42. [PubMed: 19385989] [MGI Ref ID J:148499]
Sasahara M; Otani A; Oishi A; Kojima H; Yodoi Y; Kameda T; Nakamura H; Yoshimura N. 2008. Activation of bone marrow-derived microglia promotes photoreceptor survival in inherited retinal degeneration. Am J Pathol 172(6):1693-703. [PubMed: 18483210] [MGI Ref ID J:136339]
Singh B; Wilson JH; Vasavada HH; Guo Z; Allore HG; Zeiss CJ. 2007. Motor deficits and altered striatal gene expression in aphakia (ak) mice. Brain Res 1185:283-92. [PubMed: 17949697] [MGI Ref ID J:128707]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number A1
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 18-MAY-09
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $105.90 Female or Male Homozygous for Pde6brd10
Pairs /Price (US dollars $) Pair Genotype $211.80 Homozygous for Pde6brd10 x Homozygous for Pde6brd10
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $137.70 Female or Male Homozygous for Pde6brd10
Pairs /Price (US dollars $) Pair Genotype $275.40 Homozygous for Pde6brd10 x Homozygous for Pde6brd10
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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