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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names 129S1-Hprttm1(Cre)Mnn (Changed: 15-DEC-04 ) 129S1-Hprttm1(cre)Mnn/J (Changed: 15-DEC-04 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Jeff Mann, Beckman Research Institute, City of Hope Description
Mice that are homozygous for the targeted mutation are viable and fertile. This is a Cre-deleter induced mutation strain on a 129S1 background. A Cre expression cassette was inserted into the X-linked Hprt gene. When male mice carrying a neomycin selection cassette flanked by loxP sites are mated to female mice heterozygous for this targeted mutation, the cassette is excised without detectable mosaicism, irrespective of cre inheritance. Heterozygous female mice have sufficient Cre recombinase in their oocytes to excise floxed sequence at the zygote or early cleavage stage.Development
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase 1 promoter and a Cre expression cassette containing cre driven by the cytomegalovirus early enhancer/chicken beta-globin promoter (CAG) was used to disrupt exon 3 of the targeted gene. The construct was electroporated into 129S1 derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into B6MB6F1 blastocysts. The resulting male chimeric animals were backcrossed to 129S1 mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type female from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Hprt1
002027 129S8/SvEv-Gpi1c Hprt1b-m2/J 002171 B6.129P2-Hprt1b-m3/J 008710 B6.129P2-Hprt1tm10(Ple162-EGFP/cre)Ems/J 008876 B6.129P2-Hprt1tm11(Ple176-EGFP/cre)Ems/J 008877 B6.129P2-Hprt1tm12(Ple177-EGFP/cre)Ems/J 008706 B6.129P2-Hprt1tm4(Ple88-EGFP)Ems/J 008707 B6.129P2-Hprt1tm7(Ple185-EGFP)Ems/J 008708 B6.129P2-Hprt1tm8(Ple151-EGFP)Ems/J 008709 B6.129P2-Hprt1tm9(Ple178-EGFP/cre)Ems/J 003138 B6;129-Hprt1tm1Detl/J 000807 RBJ/DnJ View Strains carrying other alleles of Hprt1 (11 strains)
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Hprt1tm1(cre)Mnn relatedResearch Tools
Cre-lox System
Metabolism Research
Neurobiology Research
Neurodegeneration
Research Tools
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Hprt1tm1(cre)Mnn | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jeffrey R Mann | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Hprtcre; Hprttm1(CAG.cre)MdCoh; hprt-cre; | ||
| Mutation Made By | Jeff Mann, Beckman Research Institute, City of Hope | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | W9.5/W95 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | widespread pattern of expression; transgenic and wildtype oocytes both confer Cre activity | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Hprt1, hypoxanthine guanine phosphoribosyl transferase 1 | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; HPGRT; HPRT; Hgprtase; MGC112554; expressed sequence C81579; | ||
| Molecular Note | An expression cassette in which a CMV enhancer and chicken beta-globin promoter driving Cre recombinase expression was inserted in reverse transcriptional orientation into exon 3 of the gene. A PGK-neomycin selection cassette was also inserted followingthe cre gene. Expression of Cre recombinase occurred at high levels in the female germline and fertilized eggs, and all offspring of heterozyous females display excision of reporter sequences. [MGI Ref ID J:75826] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Hprttm1(Cre)Mnn, STD PCR, vers. 1
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Tang SH; Silva FJ; Tsark WM; Mann JR. 2002. A cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ. Genesis 32(3):199-202. [PubMed: 11892008] [MGI Ref ID J:75826]
Additional References
Hprt1tm1(cre)Mnn relatedGu L; Tsark WM; Brown DA; Blanchard S; Synold TW; Kane SE. 2009. A new model for studying tissue-specific mdr1a gene expression in vivo by live imaging. Proc Natl Acad Sci U S A 106(13):5394-9. [PubMed: 19282474] [MGI Ref ID J:147139]
Haldar M; Hancock JD; Coffin CM; Lessnick SL; Capecchi MR. 2007. A conditional mouse model of synovial sarcoma: insights into a myogenic origin. Cancer Cell 11(4):375-88. [PubMed: 17418413] [MGI Ref ID J:120967]
Kuhnert F; Mancuso MR; Hampton J; Stankunas K; Asano T; Chen CZ; Kuo CJ. 2008. Attribution of vascular phenotypes of the murine Egfl7 locus to the microRNA miR-126. Development 135(24):3989-93. [PubMed: 18987025] [MGI Ref ID J:143206]
Ma XM; Kiraly DD; Gaier ED; Wang Y; Kim EJ; Levine ES; Eipper BA; Mains RE. 2008. Kalirin-7 is required for synaptic structure and function. J Neurosci 28(47):12368-82. [PubMed: 19020030] [MGI Ref ID J:142364]
McMahon A; Butovich IA; Mata NL; Klein M; Ritter R rd; Richardson J; Birch DG; Edwards AO; Kedzierski W. 2007. Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4. Mol Vis 13:258-72. [PubMed: 17356513] [MGI Ref ID J:121481]
Muzumdar MD; Luo L; Zong H. 2007. Modeling sporadic loss of heterozygosity in mice by using mosaic analysis with double markers (MADM). Proc Natl Acad Sci U S A 104(11):4495-500. [PubMed: 17360552] [MGI Ref ID J:120052]
Muzumdar MD; Tasic B; Miyamichi K; Li L; Luo L. 2007. A global double-fluorescent Cre reporter mouse. Genesis 45(9):593-605. [PubMed: 17868096] [MGI Ref ID J:124702]
Stark KL; Xu B; Bagchi A; Lai WS; Liu H; Hsu R; Wan X; Pavlidis P; Mills AA; Karayiorgou M; Gogos JA. 2008. Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model. Nat Genet 40(6):751-60. [PubMed: 18469815] [MGI Ref ID J:136969]
Whitehead KJ; Chan AC; Navankasattusas S; Koh W; London NR; Ling J; Mayo AH; Drakos SG; Marchuk DA; Davis GE; Li DY. 2009. The cerebral cavernous malformation signaling pathway promotes vascular integrity via Rho GTPases. Nat Med 15(2):177-84. [PubMed: 19151728] [MGI Ref ID J:146527]
Wu S; Ying G; Wu Q; Capecchi MR. 2007. Toward simpler and faster genome-wide mutagenesis in mice. Nat Genet 39(7):922-30. [PubMed: 17572674] [MGI Ref ID J:123007]
Yamamoto M; Shook NA; Kanisicak O; Yamamoto S; Wosczyna MN; Camp JR; Goldhamer DJ. 2009. A multifunctional reporter mouse line for Cre- and FLP-dependent lineage analysis. Genesis 47(2):107-14. [PubMed: 19165827] [MGI Ref ID J:147304]
Yamamoto M; Watt CD; Schmidt RJ; Kuscuoglu U; Miesfeld RL; Goldhamer DJ. 2007. Cloning and characterization of a novel MyoD enhancer-binding factor. Mech Dev 124(9-10):715-28. [PubMed: 17693064] [MGI Ref ID J:125184]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain originated and is maintained on a 129S1 background. When mating a heterozygous female to a hemizygous male, heterozygous and homozygous females, and wiltype and hemizygous males result.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type female from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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