Strain Name: |
129S1-Hprt1tm1(cre)Mnn/J |
|---|---|
Stock Number: |
004302 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
129S1-Hprttm1(Cre)Mnn (Changed: 15-DEC-04
) |
|
129S1-Hprttm1(cre)Mnn/J (Changed: 15-DEC-04
) | |
| Genes & Alleles | Hprt1; Hprt1tm1(cre)Mnn; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Jeff Mann, Beckman Research Institute, City of Hope Generation ?+N1F1p (30-MAY-04) Strain Description
Mice that are homozygous for the targeted mutation are viable and fertile. This is a Cre-deleter induced mutation strain on a 129S1 background. A Cre expression cassette was inserted into the X-linked Hprt gene. When male mice carrying a neomycin selection cassette flanked by loxP sites are mated to female mice heterozygous for this targeted mutation, the cassette is excised without detectable mosaicism, irrespective of cre inheritance. Heterozygous female mice have sufficient Cre recombinase in their oocytes to excise floxed sequence at the zygote or early cleavage stage.Strain Development
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase 1 promoter and a Cre expression cassette containing cre driven by the cytomegalovirus early enhancer/chicken beta-globin promoter (CAG) was used to disrupt exon 3 of the targeted gene. The construct was electroporated into 129S1 derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into B6MB6F1 blastocysts. The resulting male chimeric animals were backcrossed to 129S1 mice.
Gene & Allele Details
| Allele Symbol | Hprt1tm1(cre)Mnn | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jeffrey R Mann | ||
| Common Name(s) | Hprtcre; Hprttm1(CAG.cre)MdCoh; hprt-cre; | ||
| Mutation Made By | Jeff Mann, Beckman Research Institute, City of Hope | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | W9.5/W95 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | widespread pattern of expression; transgenic and wildtype oocytes both confer Cre activity | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Hprt1, hypoxanthine guanine phosphoribosyl transferase 1 | ||
| Chromosome | X | ||
| Gene Common Name(s) | C81579; HGPRT; HPGRT; HPRT; Hgprtase; MGC112554; expressed sequence C81579; | ||
| Molecular Note | An expression cassette in which a CMV enhancer and chicken beta-globin promoter driving Cre recombinase expression was inserted in reverse transcriptional orientation into exon 3 of the gene. A PGK-neomycin selection cassette was also inserted followingthe cre gene. Expression of Cre recombinase occurred at high levels in the female germline and fertilized eggs, and all offspring of heterozyous females display excision of reporter sequences. [MGI Ref ID J:75826] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type female from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Hprttm1(Cre)Mnn
Colony Maintenance
| Breeding & Husbandry | This strain originated and is maintained on a 129S1 background. When mating a heterozygous female to a hemizygous male, heterozygous and homozygous females, and wiltype and hemizygous males result. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Hprt1
002027 129S8/SvEv-Gpi1c Hprt1b-m2/J 002171 B6.129P2-Hprt1b-m3/J 003138 B6;129-Hprt1tm1Detl/J 000807 RBJ/DnJ View Strains carrying other alleles of Hprt1 (4 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Hprt1tm1(cre)Mnn relatedResearch Tools
Cre-lox System
Metabolism Research
Neurobiology Research
Neurodegeneration
Research Tools
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesTang SH; Silva FJ; Tsark WM; Mann JR. 2002. A cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ. Genesis 32(3):199-202. [PubMed: 11892008] [MGI Ref ID J:75826]
Price and Supply Information
| Strain Name: | 129S1-Hprt1tm1(cre)Mnn/J |
| Stock Number: | 004302 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $2470.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form