Strain Name:

NOD.129P2(B6)-Cd38tm1Lnd/LtJ

Stock Number:

004311

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names NOD.129P2(B6)-Cd38tm1Lnd/Lt    (Changed: 05-OCT-05 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating InvestigatorDr. Edward Leiter,   The Jackson Laboratory

Description
Congenic NOD/Lt mice homozygous for the Cd38tm1Lnd mutation (Commonly referred to as NOD.Cd38 short) are viable, fertile and exhibit accelerated Type 1 diabetes onset in females and males. This acceleration has been correlated with further impairment of an already subnormal immunoregulatory network. Regulatory T-cells from these mice are more sensitive to NAD-mediated apoptosis when compared to NOD/Lt controls. Similarly, NOD-characteristic reductions in NKT cell numbers are further exacerbated in homozygous NOD.Cd38tm1Lnd mutant mice. Although CD38 is reportedly required for normal pancreatic beta cell responses to glucose, this was not confirmed in congenic, doubly homozygous NOD.PkrdcscidCD38tm1Lnd mutant mice.

NOD.129P2(B6)-Cd38tm1Lnd/LtJ mice are valuable for understanding how systemic NAD metabolism can be used to alter immunoregulatory networks.

Development
CD38 (ADP ribosyl cyclase/cADPR hydrolase) is a Nicotinamide Adenine Dinucleotide (NAD) utilizing ectoenzyme involved in calcium mobilization. It is ubiquitously expressed to varying degrees on both hematopoietic and non-hematopoietic tissues. NAD released from target cell lysis that is not utilized by CD38 can elicit T cell apoptosis through the activity of another NAD-utilizing and T cell-specific ectoenzyme, ADP ribosyltransferase.A construct containing a neomycin expression cassette replacing exon 2 and 3 of Cd38 was transfected into E14-1 (129P2/OlaHsd-derived) embryonic stem cells (ES cells). These ES cells were injected into C57BL/6 blastocysts. As reported by Cockayne et al, 1998, chimeric founders were initially mated to C57BL/6 and intercrossed to generate homozygotes (B6.129P2-Cd38tm1Lnd). B6.129P2-Cd38tm1Lnd/J (stock# 3727)homozygote mice were subsequently mated to NOD/Lt for 11 generations prior to making homozygous. Marker assisted analysis indicates all known Idd loci are NOD/Lt in origin. In 2005, the T1DR received NOD.129P2(B6)-Cd38tm1Lnd/LtJ at generation N11F5.

Control Information

  Control
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Cd38tm1Lnd allele
003727   B6.129P2-Cd38tm1Lnd/J
005347   NOD.129(B6)-Cd38tm1Lnd Art2atm1Fkn Art2btm1Fkn/Lt
005345   NOD.Cg-Cd38tm1Lnd Prkdcscid/LtJ
View Strains carrying   Cd38tm1Lnd     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Diabetes Mellitus, Insulin-Dependent; IDDM
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Cd38tm1Lnd/Cd38tm1Lnd

        NOD.129P2(B6)-Cd38tm1Lnd/LtJ
  • immune system phenotype
  • increased T cell apoptosis
    • Cd38-deficient NOD mice display higher NAD-mediated T cell death   (MGI Ref ID J:108097)
  • increased susceptibility to autoimmune diabetes
    • homozygous NOD mutants show significant acceleration in diabetes development compared to wild-type NOD mice; the first diagnosis of diabetes is made at 10 weeks of age while in wild-type NOD mice it occurs at 12 weeks or later; the frequency in null NOD males is 100% by 28 weeks which is higher than the frequency in wild-type NOD males   (MGI Ref ID J:108097)
    • diabetes acceleration is seen only when Cd38-deficient bone marrow from NOD mice is transferred to Cd38-deficient recipients   (MGI Ref ID J:108097)
    • diabetes onset is assessed by 2 consecutive positive urine glucose tests   (MGI Ref ID J:108097)
  • insulitis
    • insulitis progression in null NOD mice is accelerated at 9 weeks or later compared to wild-type NOD mice   (MGI Ref ID J:108097)
  • endocrine/exocrine gland phenotype
  • insulitis
    • insulitis progression in null NOD mice is accelerated at 9 weeks or later compared to wild-type NOD mice   (MGI Ref ID J:108097)
  • cellular phenotype
  • increased T cell apoptosis
    • Cd38-deficient NOD mice display higher NAD-mediated T cell death   (MGI Ref ID J:108097)
  • hematopoietic system phenotype
  • increased T cell apoptosis
    • Cd38-deficient NOD mice display higher NAD-mediated T cell death   (MGI Ref ID J:108097)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cd38tm1Lnd related

Cancer Research
Genes Regulating Growth and Proliferation

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cd38tm1Lnd
Allele Name targeted mutation 1, Frances E Lund
Allele Type Targeted (Null/Knockout)
Common Name(s) CD38-; CD38null;
Mutation Made By Debra Cockayne,   Roche Bioscience
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Cd38, CD38 antigen
Chromosome 5
Gene Common Name(s) ADPRC 1; CD38 antigen, related sequence 1; Cd38-rs1; T10;
Molecular Note A neomycin selection cassette replaced a genomic fragment containing exons 2 and 3, which encode the putative active site of the encoded protein. Homozygous mice lacked transcripts derived from this allele (data not shown). Flow cytometry analysis on splenocytes derived from homozygous mice confirmed that no detectable protein was expressed on the cell surface. [MGI Ref ID J:49170]

Genotyping

Genotyping Information

Genotyping Protocols

Cd38tm1Lnd, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Chen J; Chen YG; Reifsnyder PC; Schott WH; Lee CH; Osborne M; Scheuplein F; Haag F; Koch-Nolte F; Serreze DV; Leiter EH. 2006. Targeted disruption of CD38 accelerates autoimmune diabetes in NOD/Lt mice by enhancing autoimmunity in an ADP-ribosyltransferase 2-dependent fashion. J Immunol 176(8):4590-9. [PubMed: 16585549]  [MGI Ref ID J:108097]

Chen YG; Chen J; Osborne MA; Chapman HD; Besra GS; Porcelli SA; Leiter EH; Wilson SB; Serreze DV. 2006. CD38 is required for the peripheral survival of immunotolerogenic CD4+ invariant NK T cells in nonobese diabetic mice. J Immunol 177(5):2939-47. [PubMed: 16920929]  [MGI Ref ID J:112615]

Additional References

Cd38tm1Lnd related

Adriouch S; Hubert S; Pechberty S; Koch-Nolte F; Haag F; Seman M. 2007. NAD+ released during inflammation participates in T cell homeostasis by inducing ART2-mediated death of naive T cells in vivo. J Immunol 179(1):186-94. [PubMed: 17579037]  [MGI Ref ID J:149420]

Aksoy P; White TA; Thompson M; Chini EN. 2006. Regulation of intracellular levels of NAD: A novel role for CD38. Biochem Biophys Res Commun 345(4):1386-92. [PubMed: 16730329]  [MGI Ref ID J:109655]

Barbosa MT; Soares SM; Novak CM; Sinclair D; Levine JA; Aksoy P; Chini EN. 2007. The enzyme CD38 (a NAD glycohydrolase, EC 3.2.2.5) is necessary for the development of diet-induced obesity. FASEB J 21(13):3629-39. [PubMed: 17585054]  [MGI Ref ID J:134922]

Bergthorsdottir S; Gallagher A; Jainandunsing S; Cockayne D; Sutton J; Leanderson T; Gray D. 2001. Signals that initiate somatic hypermutation of B cells in vitro. J Immunol 166(4):2228-34. [PubMed: 11160276]  [MGI Ref ID J:127146]

Ceni C; Pochon N; Villaz M; Muller-Steffner H; Schuber F; Baratier J; De Waard M; Ronjat M; Moutin MJ. 2006. The CD38-independent ADP-ribosyl cyclase from mouse brain synaptosomes: a comparative study of neonate and adult brain. Biochem J 395(2):417-26. [PubMed: 16411897]  [MGI Ref ID J:115899]

Chen YG; Scheuplein F; Driver JP; Hewes AA; Reifsnyder PC; Leiter EH; Serreze DV. 2011. Testing the Role of P2X7 Receptors in the Development of Type 1 Diabetes in Nonobese Diabetic Mice. J Immunol :. [PubMed: 21357538]  [MGI Ref ID J:169661]

Choe CU; Lardong K; Gelderblom M; Ludewig P; Leypoldt F; Koch-Nolte F; Gerloff C; Magnus T. 2011. CD38 exacerbates focal cytokine production, postischemic inflammation and brain injury after focal cerebral ischemia. PLoS One 6(5):e19046. [PubMed: 21625615]  [MGI Ref ID J:172722]

Cockayne DA; Muchamuel T; Grimaldi JC; Muller-Steffner H; Randall TD; Lund FE; Murray R; Schuber F; Howard MC. 1998. Mice deficient for the ecto-nicotinamide adenine dinucleotide Blood 92(4):1324-33. [PubMed: 9694721]  [MGI Ref ID J:49170]

Deshpande DA; White TA; Guedes AG; Milla C; Walseth TF; Lund FE; Kannan MS. 2005. Altered airway responsiveness in CD38-deficient mice. Am J Respir Cell Mol Biol 32(2):149-56. [PubMed: 15557017]  [MGI Ref ID J:107616]

Durnin L; Mutafova-Yambolieva VN. 2011. Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle. FEBS J 278(17):3095-108. [PubMed: 21740519]  [MGI Ref ID J:190791]

Gally F; Hartney JM; Janssen WJ; Perraud AL. 2009. CD38 plays a dual role in allergen-induced airway hyperresponsiveness. Am J Respir Cell Mol Biol 40(4):433-42. [PubMed: 18931329]  [MGI Ref ID J:159268]

Ge Y; Jiang W; Gan L; Wang L; Sun C; Ni P; Liu Y; Wu S; Gu L; Zheng W; Lund FE; Xin HB. 2010. Mouse embryonic fibroblasts from CD38 knockout mice are resistant to oxidative stresses through inhibition of reactive oxygen species production and Ca(2+) overload. Biochem Biophys Res Commun 399(2):167-72. [PubMed: 20638362]  [MGI Ref ID J:164600]

Guedes AG; Jude JA; Paulin J; Kita H; Lund FE; Kannan MS. 2008. Role of CD38 in TNF-alpha-induced airway hyperresponsiveness. Am J Physiol Lung Cell Mol Physiol 294(2):L290-9. [PubMed: 18055841]  [MGI Ref ID J:141542]

Guedes AG; Paulin J; Rivero-Nava L; Kita H; Lund FE; Kannan MS. 2006. CD38-deficient mice have reduced airway hyperresponsiveness following IL-13 challenge. Am J Physiol Lung Cell Mol Physiol 291(6):L1286-93. [PubMed: 16891391]  [MGI Ref ID J:144677]

Gul R; Kim SY; Park KH; Kim BJ; Kim SJ; Im MJ; Kim UH. 2008. A novel signaling pathway of ADP-ribosyl cyclase activation by angiotensin II in adult rat cardiomyocytes. Am J Physiol Heart Circ Physiol 295(1):H77-88. [PubMed: 18456728]  [MGI Ref ID J:138213]

Hubert S; Rissiek B; Klages K; Huehn J; Sparwasser T; Haag F; Koch-Nolte F; Boyer O; Seman M; Adriouch S. 2010. Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. J Exp Med 207(12):2561-8. [PubMed: 20975043]  [MGI Ref ID J:176876]

Iqbal J; Zaidi M. 2006. TNF regulates cellular NAD+ metabolism in primary macrophages. Biochem Biophys Res Commun 342(4):1312-8. [PubMed: 16516847]  [MGI Ref ID J:107073]

Johnson JD; Ford EL; Bernal-Mizrachi E; Kusser KL; Luciani DS; Han Z; Tran H; Randall TD; Lund FE; Polonsky KS. 2006. Suppressed insulin signaling and increased apoptosis in CD38-null islets. Diabetes 55(10):2737-46. [PubMed: 17003338]  [MGI Ref ID J:116576]

Kang J; Park KH; Kim JJ; Jo EK; Han MK; Kim UH. 2012. The role of CD38 in Fcgamma receptor (FcgammaR)-mediated phagocytosis in murine macrophages. J Biol Chem 287(18):14502-14. [PubMed: 22396532]  [MGI Ref ID J:184883]

Kim SY; Cho BH; Kim UH. 2010. CD38-mediated Ca2+ signaling contributes to angiotensin II-induced activation of hepatic stellate cells: attenuation of hepatic fibrosis by CD38 ablation. J Biol Chem 285(1):576-82. [PubMed: 19910464]  [MGI Ref ID J:158284]

Krebs C; Adriouch S; Braasch F; Koestner W; Leiter EH; Seman M; Lund FE; Oppenheimer N; Haag F; Koch-Nolte F. 2005. CD38 controls ADP-ribosyltransferase-2-catalyzed ADP-ribosylation of T cell surface proteins. J Immunol 174(6):3298-305. [PubMed: 15749861]  [MGI Ref ID J:97699]

Lischke T; Heesch K; Schumacher V; Schneider M; Haag F; Koch-Nolte F; Mittrucker HW. 2013. CD38 Controls the Innate Immune Response against Listeria monocytogenes. Infect Immun 81(11):4091-9. [PubMed: 23980105]  [MGI Ref ID J:201881]

Lischke T; Schumacher V; Wesolowski J; Hurwitz R; Haag F; Koch-Nolte F; Mittrucker HW. 2013. CD8-beta ADP-ribosylation affects CD8(+) T-cell function. Eur J Immunol 43(7):1828-38. [PubMed: 23575529]  [MGI Ref ID J:201025]

Liu S; Madiai F; Hackshaw KV; Allen CE; Carl J; Huschart E; Karanfilov C; Litsky A; Hickey CJ; Marcucci G; Huja S; Agarwal S; Yu J; Caligiuri MA; Wu LC. 2011. The large zinc finger protein ZAS3 is a critical modulator of osteoclastogenesis. PLoS One 6(3):e17161. [PubMed: 21390242]  [MGI Ref ID J:171725]

Manjarrez-Orduno N; Moreno-Garcia ME; Fink K; Santos-Argumedo L. 2007. CD38 cross-linking enhances TLR-induced B cell proliferation but decreases IgM plasma cell differentiation. Eur J Immunol 37(2):358-67. [PubMed: 17274001]  [MGI Ref ID J:117900]

Mayo L; Jacob-Hirsch J; Amariglio N; Rechavi G; Moutin MJ; Lund FE; Stein R. 2008. Dual role of CD38 in microglial activation and activation-induced cell death. J Immunol 181(1):92-103. [PubMed: 18566373]  [MGI Ref ID J:137179]

Moss NG; Kopple TE; Arendshorst WJ. 2014. Renal vasoconstriction by vasopressin V1a receptors is modulated by nitric oxide, prostanoids, and superoxide but not the ADP ribosyl cyclase CD38. Am J Physiol Renal Physiol 306(10):F1143-54. [PubMed: 24623148]  [MGI Ref ID J:210880]

Moss NG; Vogel PA; Kopple TE; Arendshorst WJ. 2013. Thromboxane-induced renal vasoconstriction is mediated by the ADP-ribosyl cyclase CD38 and superoxide anion. Am J Physiol Renal Physiol 305(6):F830-8. [PubMed: 23884143]  [MGI Ref ID J:200926]

Orabi AI; Muili KA; Javed TA; Jin S; Jayaraman T; Lund FE; Husain SZ. 2013. Cluster of differentiation 38 (CD38) mediates bile acid-induced acinar cell injury and pancreatitis through cyclic ADP-ribose and intracellular calcium release. J Biol Chem 288(38):27128-37. [PubMed: 23940051]  [MGI Ref ID J:203858]

Partida-Sanchez S; Cockayne DA; Monard S; Jacobson EL; Oppenheimer N; Garvy B; Kusser K; Goodrich S; Howard M; Harmsen A; Randall TD; Lund FE. 2001. Cyclic ADP-ribose production by CD38 regulates intracellular calcium release, extracellular calcium influx and chemotaxis in neutrophils and is required for bacterial clearance in vivo. Nat Med 7(11):1209-16. [PubMed: 11689885]  [MGI Ref ID J:126192]

Partida-Sanchez S; Gasser A; Fliegert R; Siebrands CC; Dammermann W; Shi G; Mousseau BJ; Sumoza-Toledo A; Bhagat H; Walseth TF; Guse AH; Lund FE. 2007. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol 179(11):7827-39. [PubMed: 18025229]  [MGI Ref ID J:155208]

Partida-Sanchez S; Goodrich S; Kusser K; Oppenheimer N; Randall TD; Lund FE. 2004. Regulation of dendritic cell trafficking by the ADP-ribosyl cyclase CD38: impact on the development of humoral immunity. Immunity 20(3):279-91. [PubMed: 15030772]  [MGI Ref ID J:89773]

Postigo J; Iglesias M; Cerezo-Wallis D; Rosal-Vela A; Garcia-Rodriguez S; Zubiaur M; Sancho J; Merino R; Merino J. 2012. Mice deficient in CD38 develop an attenuated form of collagen type II-induced arthritis. PLoS One 7(3):e33534. [PubMed: 22438945]  [MGI Ref ID J:187042]

Rodriguez-Alba JC; Moreno-Garcia ME; Sandoval-Montes C; Rosales-Garcia VH; Santos-Argumedo L. 2008. CD38 induces differentiation of immature transitional 2 B lymphocytes in the spleen. Blood 111(7):3644-52. [PubMed: 18223169]  [MGI Ref ID J:133536]

Shi G; Partida-Sanchez S; Misra RS; Tighe M; Borchers MT; Lee JJ; Simon MI; Lund FE. 2007. Identification of an alternative G{alpha}q-dependent chemokine receptor signal transduction pathway in dendritic cells and granulocytes. J Exp Med 204(11):2705-18. [PubMed: 17938235]  [MGI Ref ID J:126146]

Sun L; Iqbal J; Dolgilevich S; Yuen T; Wu XB; Moonga BS; Adebanjo OA; Bevis PJ; Lund F; Huang CL; Blair HC; Abe E; Zaidi M. 2003. Disordered osteoclast formation and function in a CD38 (ADP-ribosyl cyclase)-deficient mouse establishes an essential role for CD38 in bone resorption. FASEB J 17(3):369-75. [PubMed: 12631576]  [MGI Ref ID J:82191]

Thai TL; Arendshorst WJ. 2009. Mice lacking the ADP ribosyl cyclase CD38 exhibit attenuated renal vasoconstriction to angiotensin II, endothelin-1, and norepinephrine. Am J Physiol Renal Physiol 297(1):F169-76. [PubMed: 19403649]  [MGI Ref ID J:149837]

Xu M; Zhang Y; Xia M; Li XX; Ritter JK; Zhang F; Li PL. 2012. NAD(P)H oxidase-dependent intracellular and extracellular O(2)(*-) production in coronary arterial myocytes from CD38 knockout mice. Free Radic Biol Med 52(2):357-65. [PubMed: 22100343]  [MGI Ref ID J:179385]

Young GS; Choleris E; Lund FE; Kirkland JB. 2006. Decreased cADPR and increased NAD+ in the Cd38-/- mouse. Biochem Biophys Res Commun 346(1):188-92. [PubMed: 16750163]  [MGI Ref ID J:110441]

Zhang F; Xia M; Li PL. 2010. Lysosome-dependent Ca(2+) release response to Fas activation in coronary arterial myocytes through NAADP: evidence from CD38 gene knockouts. Am J Physiol Cell Physiol 298(5):C1209-16. [PubMed: 20200208]  [MGI Ref ID J:159245]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe accelerated diabetes development in NOD.129P2(B6)-Cd38tm1Lnd/LtJ homozygous breeding stock is not significantly retarded by either CFA or alpha galactosylceramide prophylaxis.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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