Strain Name: |
B6.129(FVB)-Gabra1tm1Geh/J |
|---|---|
Stock Number: |
004318 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
B6.129(FVB/N)-Gabra1 (Changed: 15-DEC-04
) |
| Genes & Alleles | Gabra1; Gabra1tm1Geh; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Strain Description
These mice possess loxP sites on either side of a Gabra1 exon encoding the second transmembrane domain. Mice that are homozygous for this floxed Gabra1 allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of Gabra1.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).
Strain Development
A targeting vector was constructed containing a selectable marker cassette (Neo/Tk) flanked by loxP sites. The loxP-flanked cassette was placed approximately 0.6 kb 5' of an exon encoding nucleotides 1307-1509. A third loxP- site was placed approximately 0.8 kb 3' of the same exon. The construct was electroporated into 129X1/SvJ x 129S1/Sv-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a supercoiled cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exon (F) were injected in C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and subsequently made homozygous (F/F). The homozygotes were bred to an actin-Cre general deleter on an FVB/N genetic background. Selected mice that were heterozygous for the flanked exon (F/+) and lacked the cre transgene were obtained and crossed to C57BL/6 animals.
| Allele Symbol | Gabra1tm1Geh | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Gregg E Homanics | ||
| Mutation Made By | Gregg Homanics, University of Pittsburgh | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Gabra1, gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | ECA4; EJM; GABAA alpha 1; Gabra-1; | ||
| Molecular Note | A loxP flanked neomycin and thymidine kinase cassette was inserted 5' to sequences corresponding to nucleotides 1307-1509, and a single loxP site was inserted 3' to these sequences. The loxP flanked neomycin selection cassette was removed in ES cells bytransient Cre recombinase expression prior to the production of chimeric mice, leaving a single loxP site in place of the cassette. [MGI Ref ID J:70505] | ||
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Gabra1
| Breeding & Husbandry | This strain originated on a B6;129 background, was crossed to a pure FVB/N mouse once (a Cre strain) and then crossed to C57BL/6 animals for at least 6 generations (2/02).Coat color expected from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Gabra1tm1Geh relatedNeurobiology Research
Cre-lox System (loxP-flanked Sequences)
Receptor Defects (GABA receptor)
Research Tools
Cre-lox System (loxP-flanked Sequences)
Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects
Selected Reference(s)
Additional ReferencesVicini S; Ferguson C; Prybylowski K; Kralic J; Morrow AL; Homanics GE. 2001. GABA(A) receptor alpha1 subunit deletion prevents developmental changes of inhibitory synaptic currents in cerebellar neurons. J Neurosci 21(9):3009-16. [PubMed: 11312285] [MGI Ref ID J:70505]
| Strain Name: | B6.129(FVB)-Gabra1tm1Geh/J |
| Stock Number: | 004318 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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